Restriction map for
pF1KB5448
(Circular) (Six-base) MAPSORT of: pF1KB5448.seq Check: 8873 from: 1 to: 3692
pF1KB5448 3692 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 730 730
Size: 3692
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 551 1517 1949 2302 2964 165
Size: 386 966 432 353 662 893
Fragments arranged by size:
966 893 662 432 386 353
AflIII A'CryG_T
Cuts at: 1044 2844 3125 1044
Size: 1800 281 1611
Fragments arranged by size:
1800 1611 281
AlwNI CAG_nnn'CTG
Cuts at: 997 2435 997
Size: 1438 2254
ApaLI G'TGCA_C
Cuts at: 2530 2530
Size: 3692
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 712 712
Size: 3692
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 326 359 326
Size: 33 3659
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 674 708 1122 1370 1405 674
Size: 34 414 248 35 2961
Fragments arranged by size:
2961 414 248 35 34
BanII G_rGCy'C
Cuts at: 706 1736 706
Size: 1030 2662
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BbsI GAAGACnn'nnnn_
Cuts at: 171 171
Size: 3692
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2646 3692 1590
Size: 1056 1046 1590
Fragments arranged by size:
1590 1056 1046
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 215 440 1317 1410 2534 215
Size: 225 877 93 1124 1373
Fragments arranged by size:
1373 1124 877 225 93
BmgBI CAC'GTC
Cuts at: 655 655
Size: 3692
BmrI ACTGGGnnnn_n'
Cuts at: 904 985 1320 3464 904
Size: 81 335 2144 1132
Fragments arranged by size:
2144 1132 335 81
BmtI G_CTAG'C
Cuts at: 502 502
Size: 3692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 656 2059 656
Size: 1403 2289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1747 2461 2759 868
Size: 879 714 298 1801
Fragments arranged by size:
1801 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 305 1206 3243 6
Size: 299 901 2037 455
Fragments arranged by size:
2037 901 455 299
BsaHI Gr'CG_yC
Cuts at: 1123 1371 2073 3569 1123
Size: 248 702 1496 1246
Fragments arranged by size:
1496 1246 702 248
BsaWI w'CCGG_w
Cuts at: 119 881 1402 2080 2491 2638 2912 119
Size: 762 521 678 411 147 274 899
Fragments arranged by size:
899 762 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 289 1117 1875 2540 2948 289
Size: 828 758 665 408 1033
Fragments arranged by size:
1033 828 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2510 104
Size: 1130 46 1230 1286
Fragments arranged by size:
1286 1230 1130 46
BsiHKAI G_wGCw'C
Cuts at: 485 706 1484 1674 2534 485
Size: 221 778 190 860 1643
Fragments arranged by size:
1643 860 778 221 190
Bsp1286I G_dGCh'C
Cuts at: 215 440 485 706 1317 1410 1484 1674 1736
Size: 225 45 221 611 93 74 190 62
Cuts at: 1736 2534 215
Size: 798 1373
Fragments arranged by size:
1373 798 611 225 221 190 93 74
62 45
BspEI T'CCGG_A
Cuts at: 119 119
Size: 3692
BspHI T'CATG_A
Cuts at: 2124 2124
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 742 956 1258 1639 252
Size: 490 214 302 381 2305
Fragments arranged by size:
2305 490 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 519 1690 1871 519
Size: 1171 181 2340
Fragments arranged by size:
2340 1171 181
BssSI C'ACGA_G
Cuts at: 1963 2671 1963
Size: 708 2984
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstXI CCAn_nnnn'nTGG
Cuts at: 401 447 401
Size: 46 3646
BstYI r'GATC_y
Cuts at: 1 465 717 1184 1207 1542 1788 2094 2106
Size: 464 252 467 23 335 246 306 12
Cuts at: 2106 2192 3244 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 464 449 335 306 252 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 313 313
Size: 3692
BtgI C'CryG_G
Cuts at: 107 107
Size: 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 935 935
Size: 3692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 204 239 204
Size: 35 3657
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2742 1398
Size: 1344 2348
EaeI y'GGCC_r
Cuts at: 1277 1451 1842 1869 3214 1277
Size: 174 391 27 1345 1755
Fragments arranged by size:
1755 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2642 2788 3502 1900
Size: 742 146 714 2090
Fragments arranged by size:
2090 742 714 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 551 1517 1949 2302 2964 165
Size: 386 966 432 353 662 893
Fragments arranged by size:
966 893 662 432 386 353
EcoNI CCTnn'n_nnAGG
Cuts at: 533 533
Size: 3692
EcoO109I rG'GnC_Cy
Cuts at: 317 831 317
Size: 514 3178
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
EcoRV GAT'ATC
Cuts at: 243 243
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 1711 183
Size: 1528 2164
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 566 1126 1374 2604 3375 566
Size: 560 248 1230 771 883
Fragments arranged by size:
1230 883 771 560 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1534 1564 1566 1596 1534
Size: 30 2 30 3630
Fragments arranged by size:
3630 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2451 2635 2451
Size: 184 3508
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 1681 1808 1681
Size: 127 3565
MspA1I CmG'CkG
Cuts at: 289 803 1117 1477 2261 2506 289
Size: 514 314 360 784 245 1475
Fragments arranged by size:
1475 784 514 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NcoI C'CATG_G
Cuts at: 107 107
Size: 3692
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NheI G'CTAG_C
Cuts at: 498 498
Size: 3692
NspI r_CATG'y
Cuts at: 167 745 1245 2848 167
Size: 578 500 1603 1011
Fragments arranged by size:
1603 1011 578 500
PciI A'CATG_T
Cuts at: 2844 2844
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2138 2170 2138
Size: 32 3660
PstI C_TGCA'G
Cuts at: 739 1424 739
Size: 685 3007
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 289 1117 1477 289
Size: 828 360 2504
Fragments arranged by size:
2504 828 360
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 735 1420 2388 2579 33
Size: 702 685 968 191 1146
Fragments arranged by size:
1146 968 702 685 191
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 847 1762 2476 2738 847
Size: 915 714 262 1801
Fragments arranged by size:
1801 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 107 826 1180 107
Size: 719 354 2619
Fragments arranged by size:
2619 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 662 1275 1408 1867 2076 662
Size: 613 133 459 209 2278
Fragments arranged by size:
2278 613 459 209 133
TatI w'GTAC_w
Cuts at: 3207 3207
Size: 3692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbvCI BcgI BfrBI BglI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI