Restriction map for
pF1KB5446
(Circular) (Six-base) MAPSORT of: pF1KB5446.seq Check: 6587 from: 1 to: 5078
pF1KB5446 5078 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 2116 2116
Size: 5078
Acc65I G'GTAC_C
Cuts at: 1156 2094 1156
Size: 938 4140
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3335 3688 4350 2903
Size: 432 353 662 3631
Fragments arranged by size:
3631 662 432 353
AflIII A'CryG_T
Cuts at: 2430 4230 4511 2430
Size: 1800 281 2997
Fragments arranged by size:
2997 1800 281
AleI CACnn'nnGTG
Cuts at: 1744 1744
Size: 5078
AlwNI CAG_nnn'CTG
Cuts at: 150 815 1797 1809 2383 3821 150
Size: 665 982 12 574 1438 1407
Fragments arranged by size:
1438 1407 982 665 574 12
ApaI G_GGCC'C
Cuts at: 648 992 648
Size: 344 4734
ApaLI G'TGCA_C
Cuts at: 3916 3916
Size: 5078
ApoI r'AATT_y
Cuts at: 1223 2082 4431 4687 1223
Size: 859 2349 256 1614
Fragments arranged by size:
2349 1614 859 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5078
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 157 1854 2098 157
Size: 1697 244 3137
Fragments arranged by size:
3137 1697 244
BamHI G'GATC_C
Cuts at: 1946 2103 1946
Size: 157 4921
BanI G'GyrC_C
Cuts at: 235 1056 1156 2094 2508 2756 2791 235
Size: 821 100 938 414 248 35 2522
Fragments arranged by size:
2522 938 821 414 248 100 35
BanII G_rGCy'C
Cuts at: 648 764 992 1559 1725 1997 2092 3122 648
Size: 116 228 567 166 272 95 1030 2604
Fragments arranged by size:
2604 1030 567 272 228 166 116 95
BbeI G_GCGC'C
Cuts at: 2512 2760 2512
Size: 248 4830
BbvCI CC'TCA_GC
Cuts at: 151 1633 151
Size: 1482 3596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 100 124 134 1816 1850 90
Size: 10 24 10 1682 34 3318
Fragments arranged by size:
3318 1682 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 2976 4032 5078 2976
Size: 1056 1046 2976
Fragments arranged by size:
2976 1056 1046
BclI T'GATC_A
Cuts at: 2598 2598
Size: 5078
BfrBI ATG'CAT
Cuts at: 615 1406 615
Size: 791 4287
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5078
BglII A'GATC_T
Cuts at: 1467 2593 4630 1467
Size: 1126 2037 1915
Fragments arranged by size:
2037 1915 1126
BlpI GC'TnA_GC
Cuts at: 242 2190 242
Size: 1948 3130
Bme1580I G_kGCm'C
Cuts at: 548 648 809 992 2703 2796 3920 548
Size: 100 161 183 1711 93 1124 1706
Fragments arranged by size:
1711 1706 1124 183 161 100 93
BmrI ACTGGGnnnn_n'
Cuts at: 542 576 1203 2290 2371 2706 4850 542
Size: 34 627 1087 81 335 2144 770
Fragments arranged by size:
2144 1087 770 627 335 81 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 5078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1466 1747 502
Size: 964 281 3833
Fragments arranged by size:
3833 964 281
Bpu10I CC'TnA_GC
Cuts at: 151 1178 1633 151
Size: 1027 455 3596
Fragments arranged by size:
3596 1027 455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 750 1848 2254 3133 3847 4145 499
Size: 251 1098 406 879 714 298 1432
Fragments arranged by size:
1432 1098 879 714 406 298 251
BsaI GGTCTCn'nnnn_
Cuts at: 490 4782 490
Size: 4292 786
BsaAI yAC'GTr
Cuts at: 2431 2431
Size: 5078
BsaBI GATnn'nnATC
Cuts at: 6 1009 2592 4629 6
Size: 1003 1583 2037 455
Fragments arranged by size:
2037 1583 1003 455
BsaHI Gr'CG_yC
Cuts at: 2509 2757 3459 4955 2509
Size: 248 702 1496 2632
Fragments arranged by size:
2632 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1481 2267 2788 3466 3877 4024 4298 1481
Size: 786 521 678 411 147 274 2261
Fragments arranged by size:
2261 786 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 1040 1070 418
Size: 30 592 30 4426
Fragments arranged by size:
4426 592 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 508
Size: 5078
BseYI C'CCAG_C
Cuts at: 522 555 808 1074 1650 1895 2503 3261 3926
Size: 33 253 266 576 245 608 758 665
Cuts at: 3926 4334 522
Size: 408 1266
Fragments arranged by size:
1266 758 665 608 576 408 266 253
245 33
BsiEI CG_ry'CG
Cuts at: 104 2620 2666 3896 104
Size: 2516 46 1230 1286
Fragments arranged by size:
2516 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 389 1463 1559 1578 1997 2092 2870 3060 3920
Size: 1074 96 19 419 95 778 190 860
Cuts at: 3920 389
Size: 1547
Fragments arranged by size:
1547 1074 860 778 419 190 96 95
19
BsmI GAATG_Cn'
Cuts at: 1408 1602 1408
Size: 194 4884
Bsp1286I G_dGCh'C
Cuts at: 389 548 648 764 809 992 1463 1559 1578
Size: 159 100 116 45 183 471 96 19
Cuts at: 1578 1725 1997 2092 2703 2796 2870 3060 3122
Size: 147 272 95 611 93 74 190 62
Cuts at: 3122 3920 389
Size: 798 1547
Fragments arranged by size:
1547 798 611 471 272 190 183 159
147 116 100 96 95 93 74 62
45 19
BspEI T'CCGG_A
Cuts at: 1481 1481
Size: 5078
BspHI T'CATG_A
Cuts at: 1433 3510 1433
Size: 2077 3001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1144 2128 2342 2644 3025 1144
Size: 984 214 302 381 3197
Fragments arranged by size:
3197 984 381 302 214
BsrBI CCG'CTC
Cuts at: 50 241 2037 3370 3424 4908 5073 50
Size: 191 1796 1333 54 1484 165 55
Fragments arranged by size:
1796 1484 1333 191 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1172 2990 1172
Size: 1818 3260
BsrFI r'CCGG_y
Cuts at: 300 3076 3257 300
Size: 2776 181 2121
Fragments arranged by size:
2776 2121 181
BssSI C'ACGA_G
Cuts at: 3349 4057 3349
Size: 708 4370
BstAPI GCAn_nnn'nTGC
Cuts at: 2455 2455
Size: 5078
BstBI TT'CG_AA
Cuts at: 3439 3439
Size: 5078
BstEII G'GTnAC_C
Cuts at: 2049 2049
Size: 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 555 1746 1845 2028 555
Size: 1191 99 183 3605
Fragments arranged by size:
3605 1191 183 99
BstYI r'GATC_y
Cuts at: 1 912 1467 1484 1867 1946 2103 2570 2593
Size: 911 555 17 383 79 157 467 23
Cuts at: 2593 2928 3174 3480 3492 3578 4630 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 911 555 467 449 383 335 306
246 157 86 79 23 17 12
BtgI C'CryG_G
Cuts at: 984 1292 1331 1838 1889 984
Size: 308 39 507 51 4173
Fragments arranged by size:
4173 507 308 51 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3028 3209 3372 3028
Size: 181 163 4734
Fragments arranged by size:
4734 181 163
BtsI GCAGTG_nn'
Cuts at: 1578 2321 1578
Size: 743 4335
DraI TTT'AAA
Cuts at: 2077 2077
Size: 5078
DraIII CAC_nnn'GTG
Cuts at: 1925 1925
Size: 5078
DrdI GACnn_nn'nnGTC
Cuts at: 2784 4128 2784
Size: 1344 3734
EaeI y'GGCC_r
Cuts at: 125 302 740 1961 2663 2837 3228 3255 4600
Size: 177 438 1221 702 174 391 27 1345
Cuts at: 4600 125
Size: 603
Fragments arranged by size:
1345 1221 702 603 438 391 177 174
27
EagI C'GGCC_G
Cuts at: 2663 2663
Size: 5078
EarI CTCTTCn'nnn_
Cuts at: 389 3101 3311 389
Size: 2712 210 2156
Fragments arranged by size:
2712 2156 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 179 325 3286 4028 4174 4888 179
Size: 146 2961 742 146 714 369
Fragments arranged by size:
2961 742 714 369 146 146
EcoICRI GAG'CTC
Cuts at: 1557 1995 2090 1557
Size: 438 95 4545
Fragments arranged by size:
4545 438 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1466 1747 2903 3335 3688 4350 502
Size: 964 281 1156 432 353 662 1230
Fragments arranged by size:
1230 1156 964 662 432 353 281
EcoNI CCTnn'n_nnAGG
Cuts at: 1876 1876
Size: 5078
EcoO109I rG'GnC_Cy
Cuts at: 510 527 644 867 989 1859 2217 510
Size: 17 117 223 122 870 358 3371
Fragments arranged by size:
3371 870 358 223 122 117 17
EcoRI G'AATT_C
Cuts at: 2082 2082
Size: 5078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1605 3097 1605
Size: 1492 3586
FspI TGC'GCA
Cuts at: 2859 2859
Size: 5078
HaeII r_GCGC'y
Cuts at: 187 2512 2760 3990 4761 187
Size: 2325 248 1230 771 504
Fragments arranged by size:
2325 1230 771 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 769 801 2920 2950 2952 2982 418
Size: 32 319 32 2119 30 2 30 2514
Fragments arranged by size:
2514 2119 319 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2117 2117
Size: 5078
HindIII A'AGCT_T
Cuts at: 847 847
Size: 5078
KasI G'GCGC_C
Cuts at: 2508 2756 2508
Size: 248 4830
KpnI G_GTAC'C
Cuts at: 1160 2098 1160
Size: 938 4140
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1678 3837 4021 1678
Size: 2159 184 2735
Fragments arranged by size:
2735 2159 184
MscI TGG'CCA
Cuts at: 2839 2839
Size: 5078
MslI CAynn'nnrTG
Cuts at: 1744 1843 3067 3194 1744
Size: 99 1224 127 3628
Fragments arranged by size:
3628 1224 127 99
MspA1I CmG'CkG
Cuts at: 246 1632 1658 1673 1806 2189 2503 2863 3647
Size: 1386 26 15 133 383 314 360 784
Cuts at: 3647 3892 246
Size: 245 1432
Fragments arranged by size:
1432 1386 784 383 360 314 245 133
26 15
NaeI GCC'GGC
Cuts at: 3259 3259
Size: 5078
NarI GG'CG_CC
Cuts at: 2509 2757 2509
Size: 248 4830
NcoI C'CATG_G
Cuts at: 984 1292 1838 984
Size: 308 546 4224
Fragments arranged by size:
4224 546 308
NgoMIV G'CCGG_C
Cuts at: 3257 3257
Size: 5078
NsiI A_TGCA'T
Cuts at: 617 1408 617
Size: 791 4287
NspI r_CATG'y
Cuts at: 615 2131 2631 4234 615
Size: 1516 500 1603 1459
Fragments arranged by size:
1603 1516 1459 500
PasI CC'CwG_GG
Cuts at: 639 639
Size: 5078
PciI A'CATG_T
Cuts at: 4230 4230
Size: 5078
PflMI CCAn_nnn'nTGG
Cuts at: 1416 1416
Size: 5078
PfoI T'CCnGG_A
Cuts at: 1244 1244
Size: 5078
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 5046
PspOMI G'GGCC_C
Cuts at: 644 988 644
Size: 344 4734
PstI C_TGCA'G
Cuts at: 1277 1532 1805 1941 1973 2125 2810 1277
Size: 255 273 136 32 152 685 3545
Fragments arranged by size:
3545 685 273 255 152 136 32
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5078
PvuII CAG'CTG
Cuts at: 246 1632 2503 2863 246
Size: 1386 871 360 2461
Fragments arranged by size:
2461 1386 871 360
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 1559 1997 2092 1559
Size: 438 95 4545
Fragments arranged by size:
4545 438 95
SalI G'TCGA_C
Cuts at: 2115 2115
Size: 5078
SapI GCTCTTCn'nnn_
Cuts at: 389 3101 3311 389
Size: 2712 210 2156
Fragments arranged by size:
2712 2156 210
SbfI CC_TGCA'GG
Cuts at: 2125 2125
Size: 5078
ScaI AGT'ACT
Cuts at: 4595 4595
Size: 5078
SexAI A'CCwGG_T
Cuts at: 1921 1921
Size: 5078
SfcI C'TryA_G
Cuts at: 33 1273 1528 1801 1937 1969 2121 2806 3774
Size: 1240 255 273 136 32 152 685 968
Cuts at: 3774 3965 33
Size: 191 1146
Fragments arranged by size:
1240 1146 968 685 273 255 191 152
136 32
SfoI GGC'GCC
Cuts at: 2510 2758 2510
Size: 248 4830
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 2100 2100
Size: 5078
SmlI C'TyrA_G
Cuts at: 514 765 1854 1863 2233 3148 3862 4124 514
Size: 251 1089 9 370 915 714 262 1468
Fragments arranged by size:
1468 1089 915 714 370 262 251 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5078
SphI G_CATG'C
Cuts at: 615 2131 2631 615
Size: 1516 500 3062
Fragments arranged by size:
3062 1516 500
StuI AGG'CCT
Cuts at: 2026 2026
Size: 5078
StyI C'CwwG_G
Cuts at: 305 621 984 1292 1838 2021 2027 2212 2566
Size: 316 363 308 546 183 6 185 354
Cuts at: 2566 305
Size: 2817
Fragments arranged by size:
2817 546 363 354 316 308 185 183
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 547 595 1066 2661 2794 3253 3462 547
Size: 48 471 1595 133 459 209 2163
Fragments arranged by size:
2163 1595 471 459 209 133 48
TatI w'GTAC_w
Cuts at: 4593 4593
Size: 5078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1040 1072 4595 4627 1040
Size: 32 3523 32 1491
Fragments arranged by size:
3523 1491 32 32
Tth111I GACn'n_nGTC
Cuts at: 2875 2875
Size: 5078
XbaI T'CTAG_A
Cuts at: 2109 2109
Size: 5078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1299 2028 2573 1299
Size: 729 545 3804
Fragments arranged by size:
3804 729 545
XhoI C'TCGA_G
Cuts at: 1854 1854
Size: 5078
XmaI C'CCGG_G
Cuts at: 2098 2098
Size: 5078
XmnI GAAnn'nnTTC
Cuts at: 1793 1793
Size: 5078
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbsI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI