Restriction map for
pF1KB5445
(Circular) (Six-base) MAPSORT of: pF1KB5445.seq Check: 5554 from: 1 to: 3926
pF1KB5445 3926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 267 904 267
Size: 637 3289
AatII G_ACGT'C
Cuts at: 827 827
Size: 3926
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 300 896 1231 2798 300
Size: 596 335 1567 1428
Fragments arranged by size:
1567 1428 596 335
ApaI G_GGCC'C
Cuts at: 132 205 241 774 132
Size: 73 36 533 3284
Fragments arranged by size:
3284 533 73 36
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 930 3279 3535 930
Size: 2349 256 1321
Fragments arranged by size:
2349 1321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 137 219 234 946 137
Size: 82 15 712 3117
Fragments arranged by size:
3117 712 82 15
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 490 741 942 1356 1604 1639 490
Size: 251 201 414 248 35 2777
Fragments arranged by size:
2777 414 251 248 201 35
BanII G_rGCy'C
Cuts at: 132 205 212 241 634 774 940 1970 132
Size: 73 7 29 393 140 166 1030 2088
Fragments arranged by size:
2088 1030 393 166 140 73 29 7
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbvCI CC'TCA_GC
Cuts at: 721 721
Size: 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3892
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglI GCCn_nnn'nGGC
Cuts at: 676 676
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 132 138 205 241 435 686 746 774 1551
Size: 6 67 36 194 251 60 28 777
Cuts at: 1551 1644 2700 132
Size: 93 1056 1358
Fragments arranged by size:
1358 1056 777 251 194 93 67 60
36 28 6
BmgBI CAC'GTC
Cuts at: 816 816
Size: 3926
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 873 838
Size: 35 3891
Bpu10I CC'TnA_GC
Cuts at: 721 721
Size: 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 3926
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 907 1440 3477 6
Size: 901 533 2037 455
Fragments arranged by size:
2037 901 533 455
BsaHI Gr'CG_yC
Cuts at: 285 418 824 1357 1605 2307 3803 285
Size: 133 406 533 248 702 1496 408
Fragments arranged by size:
1496 702 533 408 406 248 133
BsaWI w'CCGG_w
Cuts at: 1115 1636 2314 2588 2735 3146 1115
Size: 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 814 844 814
Size: 30 3896
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 322
Size: 3926
BseYI C'CCAG_C
Cuts at: 393 634 1351 2109 2686 3182 393
Size: 241 717 758 577 496 1137
Fragments arranged by size:
1137 758 717 577 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 3926
BsiEI CG_ry'CG
Cuts at: 104 680 807 1468 1514 2722 104
Size: 576 127 661 46 1208 1308
Fragments arranged by size:
1308 1208 661 576 127 46
BsiHKAI G_wGCw'C
Cuts at: 542 767 940 1718 1908 2700 542
Size: 225 173 778 190 792 1768
Fragments arranged by size:
1768 792 778 225 190 173
BsiWI C'GTAC_G
Cuts at: 620 620
Size: 3926
Bsp1286I G_dGCh'C
Cuts at: 132 138 205 212 241 435 542 634 686
Size: 6 67 7 29 194 107 92 52
Cuts at: 686 746 767 774 940 1551 1644 1718 1908
Size: 60 21 7 166 611 93 74 190
Cuts at: 1908 1970 2700 132
Size: 62 730 1358
Fragments arranged by size:
1358 730 611 194 190 166 107 93
92 74 67 62 60 52 29 21
7 7 6
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 542 575 3102 542
Size: 33 2527 1366
Fragments arranged by size:
2527 1366 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 267 904 976 1190 1492 1873 267
Size: 637 72 214 302 381 2320
Fragments arranged by size:
2320 637 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 437 2218 2272 3756 3921 50
Size: 387 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 387 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 526 586 1924 2105 2314 526
Size: 60 1338 181 209 2138
Fragments arranged by size:
2138 1338 209 181 60
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 393 642 393
Size: 249 3677
BstYI r'GATC_y
Cuts at: 1 951 1418 1441 1776 2022 3034 3120 3132
Size: 950 467 23 335 246 1012 86 12
Cuts at: 3132 3478 1
Size: 346 449
Fragments arranged by size:
1012 950 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 440 468 656 701 440
Size: 28 188 45 3665
Fragments arranged by size:
3665 188 45 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 330 1876 2057 2220 330
Size: 1546 181 163 2036
Fragments arranged by size:
2036 1546 181 163
BtsI GCAGTG_nn'
Cuts at: 1169 1169
Size: 3926
ClaI AT'CG_AT
Cuts at: 371 371
Size: 3926
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DraIII CAC_nnn'GTG
Cuts at: 732 732
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 365 408 524 528 584 677 804 1511 1685
Size: 43 116 4 56 93 127 707 174
Cuts at: 1685 2076 2103 3448 365
Size: 391 27 1345 843
Fragments arranged by size:
1345 843 707 391 174 127 116 93
56 43 27 4
EagI C'GGCC_G
Cuts at: 677 804 1511 677
Size: 127 707 3092
Fragments arranged by size:
3092 707 127
EarI CTCTTCn'nnn_
Cuts at: 505 1949 2159 505
Size: 1444 210 2272
Fragments arranged by size:
2272 1444 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 873 1751 2183 2930 3198 838
Size: 35 878 432 747 268 1566
Fragments arranged by size:
1566 878 747 432 268 35
EcoO109I rG'GnC_Cy
Cuts at: 201 771 1065 201
Size: 570 294 3062
Fragments arranged by size:
3062 570 294
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
EcoRV GAT'ATC
Cuts at: 375 375
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FseI GG_CCGG'CC
Cuts at: 530 530
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2630 3609 1360
Size: 248 1022 979 1677
Fragments arranged by size:
1677 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 479 511 814 846 1768 1798 1800
Size: 32 287 32 303 32 922 30 2
Cuts at: 1800 1830 160
Size: 30 2256
Fragments arranged by size:
2256 922 303 287 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2781 2597
Size: 184 3742
MscI TGG'CCA
Cuts at: 367 1687 367
Size: 1320 2606
MslI CAynn'nnrTG
Cuts at: 580 1915 2042 580
Size: 1335 127 2464
Fragments arranged by size:
2464 1335 127
MspA1I CmG'CkG
Cuts at: 300 324 1037 1351 1711 2724 2969 300
Size: 24 713 314 360 1013 245 1257
Fragments arranged by size:
1257 1013 713 360 314 245 24
NaeI GCC'GGC
Cuts at: 528 588 2107 528
Size: 60 1519 2347
Fragments arranged by size:
2347 1519 60
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NcoI C'CATG_G
Cuts at: 440 656 701 440
Size: 216 45 3665
Fragments arranged by size:
3665 216 45
NgoMIV G'CCGG_C
Cuts at: 526 586 2105 526
Size: 60 1519 2347
Fragments arranged by size:
2347 1519 60
NspI r_CATG'y
Cuts at: 321 979 1479 2386 321
Size: 658 500 907 1861
Fragments arranged by size:
1861 907 658 500
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PflMI CCAn_nnn'nTGG
Cuts at: 262 262
Size: 3926
PfoI T'CCnGG_A
Cuts at: 566 641 566
Size: 75 3851
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PshAI GACnn'nnGTC
Cuts at: 288 288
Size: 3926
PspOMI G'GGCC_C
Cuts at: 128 201 237 770 128
Size: 73 36 533 3284
Fragments arranged by size:
3284 533 73 36
PstI C_TGCA'G
Cuts at: 973 1658 973
Size: 685 3241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 300 324 1351 1711 300
Size: 24 1027 360 2515
Fragments arranged by size:
2515 1027 360 24
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 505 1949 2159 505
Size: 1444 210 2272
Fragments arranged by size:
2272 1444 210
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SexAI A'CCwGG_T
Cuts at: 728 728
Size: 3926
SfcI C'TryA_G
Cuts at: 33 969 1654 2647 2838 33
Size: 936 685 993 191 1121
Fragments arranged by size:
1121 993 936 685 191
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 139 948 139
Size: 809 3117
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 321 979 1479 321
Size: 658 500 2768
Fragments arranged by size:
2768 658 500
StuI AGG'CCT
Cuts at: 232 232
Size: 3926
StyI C'CwwG_G
Cuts at: 194 440 656 701 1060 1414 194
Size: 246 216 45 359 354 2706
Fragments arranged by size:
2706 359 354 246 216 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 1642 2101 2310 1509
Size: 133 459 209 3125
Fragments arranged by size:
3125 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 1421 501
Size: 920 3006
XmaI C'CCGG_G
Cuts at: 137 946 137
Size: 809 3117
ZraI GAC'GTC
Cuts at: 825 825
Size: 3926
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmtI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I CspCI EcoNI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI