Restriction map for
pF1KB5444
(Circular) (Six-base) MAPSORT of: pF1KB5444.seq Check: 1723 from: 1 to: 5285
pF1KB5444 5285 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1575 1575
Size: 5285
AccI GT'mk_AC
Cuts at: 368 2323 368
Size: 1955 3330
Acc65I G'GTAC_C
Cuts at: 640 2301 640
Size: 1661 3624
AclI AA'CG_TT
Cuts at: 5125 5125
Size: 5285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 3110 3542 3895 4557 442
Size: 2668 432 353 662 1170
Fragments arranged by size:
2668 1170 662 432 353
AflIII A'CryG_T
Cuts at: 2637 4437 4718 2637
Size: 1800 281 3204
Fragments arranged by size:
3204 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 2221 2590 4028 2221
Size: 369 1438 3478
Fragments arranged by size:
3478 1438 369
ApaLI G'TGCA_C
Cuts at: 121 4123 121
Size: 4002 1283
ApoI r'AATT_y
Cuts at: 1015 1090 1390 1687 2289 4638 4894 1015
Size: 75 300 297 602 2349 256 1406
Fragments arranged by size:
2349 1406 602 300 297 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 5285
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
AvaI C'yCGr_G
Cuts at: 1280 2239 2305 1280
Size: 959 66 4260
Fragments arranged by size:
4260 959 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 632 665 1207 1240 632
Size: 33 542 33 4677
Fragments arranged by size:
4677 542 33 33
BamHI G'GATC_C
Cuts at: 2121 2310 2121
Size: 189 5096
BanI G'GyrC_C
Cuts at: 640 2301 2715 2963 2998 640
Size: 1661 414 248 35 2927
Fragments arranged by size:
2927 1661 414 248 35
BanII G_rGCy'C
Cuts at: 629 1125 1352 1655 2299 3329 629
Size: 496 227 303 644 1030 2585
Fragments arranged by size:
2585 1030 644 496 303 227
BbeI G_GCGC'C
Cuts at: 2719 2967 2719
Size: 248 5037
BbsI GAAGACnn'nnnn_
Cuts at: 575 1052 1376 1442 1856 575
Size: 477 324 66 414 4004
Fragments arranged by size:
4004 477 414 324 66
BbvCI CC'TCA_GC
Cuts at: 2033 2033
Size: 5285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2236 2270 90
Size: 34 2112 34 3105
Fragments arranged by size:
3105 2112 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3183 4239 5285 3183
Size: 1056 1046 3183
Fragments arranged by size:
3183 1056 1046
BclI T'GATC_A
Cuts at: 542 939 2156 2805 542
Size: 397 1217 649 3022
Fragments arranged by size:
3022 1217 649 397
BglI GCCn_nnn'nGGC
Cuts at: 160 160
Size: 5285
BglII A'GATC_T
Cuts at: 2800 4837 2800
Size: 2037 3248
BlpI GC'TnA_GC
Cuts at: 2397 2397
Size: 5285
Bme1580I G_kGCm'C
Cuts at: 125 2910 3003 4127 125
Size: 2785 93 1124 1283
Fragments arranged by size:
2785 1283 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1050 1482 2497 2578 2913 5057 1050
Size: 432 1015 81 335 2144 1278
Fragments arranged by size:
2144 1278 1015 432 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 1306 1371 1422 3652 255
Size: 1051 65 51 2230 1888
Fragments arranged by size:
2230 1888 1051 65 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1276 1514 1750 2005 611
Size: 665 238 236 255 3891
Fragments arranged by size:
3891 665 255 238 236
Bpu10I CC'TnA_GC
Cuts at: 2033 2033
Size: 5285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 1402 1666 2137 2461 3340 4054 4352 1366
Size: 36 264 471 324 879 714 298 2299
Fragments arranged by size:
2299 879 714 471 324 298 264 36
BsaI GGTCTCn'nnnn_
Cuts at: 1713 4989 1713
Size: 3276 2009
BsaAI yAC'GTr
Cuts at: 2638 2638
Size: 5285
BsaBI GATnn'nnATC
Cuts at: 6 2799 4836 6
Size: 2793 2037 455
Fragments arranged by size:
2793 2037 455
BsaHI Gr'CG_yC
Cuts at: 2716 2964 3666 5162 2716
Size: 248 702 1496 2839
Fragments arranged by size:
2839 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2474 2995 3673 4084 4231 4505 2474
Size: 521 678 411 147 274 3254
Fragments arranged by size:
3254 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1423 1453 1423
Size: 30 5255
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 151 892 1018 1762 1765 148
Size: 3 741 126 744 3 3668
Fragments arranged by size:
3668 744 741 126 3 3
BseYI C'CCAG_C
Cuts at: 2710 3468 4133 4541 2710
Size: 758 665 408 3454
Fragments arranged by size:
3454 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1693 488
Size: 1205 4080
BsiEI CG_ry'CG
Cuts at: 104 2827 2873 4103 104
Size: 2723 46 1230 1286
Fragments arranged by size:
2723 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 125 1352 2299 3077 3267 4127 125
Size: 1227 947 778 190 860 1283
Fragments arranged by size:
1283 1227 947 860 778 190
BsmI GAATG_Cn'
Cuts at: 442 720 1402 442
Size: 278 682 4325
Fragments arranged by size:
4325 682 278
BsmBI CGTCTCn'nnnn_
Cuts at: 2008 2264 2008
Size: 256 5029
Bsp1286I G_dGCh'C
Cuts at: 125 629 1125 1352 1655 2299 2910 3003 3077
Size: 504 496 227 303 644 611 93 74
Cuts at: 3077 3267 3329 4127 125
Size: 190 62 798 1283
Fragments arranged by size:
1283 798 644 611 504 496 303 227
190 93 74 62
BspHI T'CATG_A
Cuts at: 1805 1922 3717 1805
Size: 117 1795 3373
Fragments arranged by size:
3373 1795 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1575 2335 2549 2851 3232 1575
Size: 760 214 302 381 3628
Fragments arranged by size:
3628 760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1917 3577 3631 5115 5280 50
Size: 1867 1660 54 1484 165 55
Fragments arranged by size:
1867 1660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 419 1099 3197 419
Size: 680 2098 2507
Fragments arranged by size:
2507 2098 680
BsrFI r'CCGG_y
Cuts at: 3283 3464 3283
Size: 181 5104
BssSI C'ACGA_G
Cuts at: 3556 4264 3556
Size: 708 4577
BstAPI GCAn_nnn'nTGC
Cuts at: 169 2662 169
Size: 2493 2792
BstBI TT'CG_AA
Cuts at: 295 3646 295
Size: 3351 1934
BstYI r'GATC_y
Cuts at: 1 1260 2121 2228 2310 2777 2800 3135 3381
Size: 1259 861 107 82 467 23 335 246
Cuts at: 3381 3687 3699 3785 4837 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1259 1052 861 467 449 335 306 246
107 86 82 23 12
Bsu36I CC'TnA_GG
Cuts at: 113 344 1739 113
Size: 231 1395 3659
Fragments arranged by size:
3659 1395 231
BtgI C'CryG_G
Cuts at: 126 618 1239 1955 126
Size: 492 621 716 3456
Fragments arranged by size:
3456 716 621 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2261 3235 3416 3579 2261
Size: 974 181 163 3967
Fragments arranged by size:
3967 974 181 163
BtsI GCAGTG_nn'
Cuts at: 2528 2528
Size: 5285
DraI TTT'AAA
Cuts at: 2284 2284
Size: 5285
DrdI GACnn_nn'nnGTC
Cuts at: 1512 1548 2991 4335 1512
Size: 36 1443 1344 2462
Fragments arranged by size:
2462 1443 1344 36
EaeI y'GGCC_r
Cuts at: 1589 1970 2870 3044 3435 3462 4807 1589
Size: 381 900 174 391 27 1345 2067
Fragments arranged by size:
2067 1345 900 391 381 174 27
EagI C'GGCC_G
Cuts at: 2870 2870
Size: 5285
EarI CTCTTCn'nnn_
Cuts at: 461 683 797 815 1357 1693 1763 3308 3518
Size: 222 114 18 542 336 70 1545 210
Cuts at: 3518 461
Size: 2228
Fragments arranged by size:
2228 1545 542 336 222 210 114 70
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1168 3493 4235 4381 5095 1168
Size: 2325 742 146 714 1358
Fragments arranged by size:
2325 1358 742 714 146
EcoICRI GAG'CTC
Cuts at: 1350 2297 1350
Size: 947 4338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 611 1276 1514 1750 2005 3110 3542 3895
Size: 169 665 238 236 255 1105 432 353
Cuts at: 3895 4557 442
Size: 662 1170
Fragments arranged by size:
1170 1105 665 662 432 353 255 238
236 169
EcoO109I rG'GnC_Cy
Cuts at: 2064 2424 2064
Size: 360 4925
EcoRI G'AATT_C
Cuts at: 1015 1090 2289 1015
Size: 75 1199 4011
Fragments arranged by size:
4011 1199 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 5285
FspI TGC'GCA
Cuts at: 3066 3066
Size: 5285
HaeII r_GCGC'y
Cuts at: 2719 2967 4197 4968 2719
Size: 248 1230 771 3036
Fragments arranged by size:
3036 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 642 674 1492 1494 1524 1526 2220 2242 2252
Size: 32 818 2 30 2 694 22 10
Cuts at: 2252 2274 3127 3157 3159 3189 642
Size: 22 853 30 2 30 2738
Fragments arranged by size:
2738 853 818 694 32 30 30 30
22 22 10 2 2 2
HincII GTy'rAC
Cuts at: 1251 2324 1251
Size: 1073 4212
HindIII A'AGCT_T
Cuts at: 1420 1420
Size: 5285
KasI G'GCGC_C
Cuts at: 2715 2963 2715
Size: 248 5037
KpnI G_GTAC'C
Cuts at: 644 2305 644
Size: 1661 3624
MluI A'CGCG_T
Cuts at: 4718 4718
Size: 5285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 281 1067 2162 4044 4228 233
Size: 48 786 1095 1882 184 1290
Fragments arranged by size:
1882 1290 1095 786 184 48
MscI TGG'CCA
Cuts at: 1591 1972 3046 1591
Size: 381 1074 3830
Fragments arranged by size:
3830 1074 381
MslI CAynn'nnrTG
Cuts at: 382 478 557 3274 3401 382
Size: 96 79 2717 127 2266
Fragments arranged by size:
2717 2266 127 96 79
MspA1I CmG'CkG
Cuts at: 890 1202 2396 2710 3070 3854 4099 890
Size: 312 1194 314 360 784 245 2076
Fragments arranged by size:
2076 1194 784 360 314 312 245
NaeI GCC'GGC
Cuts at: 3466 3466
Size: 5285
NarI GG'CG_CC
Cuts at: 2716 2964 2716
Size: 248 5037
NcoI C'CATG_G
Cuts at: 126 618 1955 126
Size: 492 1337 3456
Fragments arranged by size:
3456 1337 492
NgoMIV G'CCGG_C
Cuts at: 3464 3464
Size: 5285
NspI r_CATG'y
Cuts at: 2338 2838 4441 2338
Size: 500 1603 3182
Fragments arranged by size:
3182 1603 500
PciI A'CATG_T
Cuts at: 4437 4437
Size: 5285
PmeI GTTT'AAAC
Cuts at: 2284 2284
Size: 5285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 3731
Size: 32 5253
PpuMI rG'GwC_Cy
Cuts at: 2064 2064
Size: 5285
PshAI GACnn'nnGTC
Cuts at: 1860 1860
Size: 5285
PstI C_TGCA'G
Cuts at: 2217 2332 3017 2217
Size: 115 685 4485
Fragments arranged by size:
4485 685 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5285
PvuII CAG'CTG
Cuts at: 1202 2710 3070 1202
Size: 1508 360 3417
Fragments arranged by size:
3417 1508 360
RsrII CG'GwC_CG
Cuts at: 3480 3480
Size: 5285
SacI G_AGCT'C
Cuts at: 1352 2299 1352
Size: 947 4338
SalI G'TCGA_C
Cuts at: 2322 2322
Size: 5285
SapI GCTCTTCn'nnn_
Cuts at: 461 1357 1693 3308 3518 461
Size: 896 336 1615 210 2228
Fragments arranged by size:
2228 1615 896 336 210
SbfI CC_TGCA'GG
Cuts at: 2332 2332
Size: 5285
ScaI AGT'ACT
Cuts at: 1666 4802 1666
Size: 3136 2149
SexAI A'CCwGG_T
Cuts at: 2066 2066
Size: 5285
SfcI C'TryA_G
Cuts at: 33 1073 2213 2328 3013 3981 4172 33
Size: 1040 1140 115 685 968 191 1146
Fragments arranged by size:
1146 1140 1040 968 685 191 115
SfoI GGC'GCC
Cuts at: 2717 2965 2717
Size: 248 5037
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
SmaI CCC'GGG
Cuts at: 2307 2307
Size: 5285
SmlI C'TyrA_G
Cuts at: 1345 1417 1681 2116 2239 2440 3355 4069 4331
Size: 72 264 435 123 201 915 714 262
Cuts at: 4331 1345
Size: 2299
Fragments arranged by size:
2299 915 714 435 264 262 201 123
72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5285
SphI G_CATG'C
Cuts at: 2338 2838 2338
Size: 500 4785
StyI C'CwwG_G
Cuts at: 126 257 618 1718 1955 2419 2773 126
Size: 131 361 1100 237 464 354 2638
Fragments arranged by size:
2638 1100 464 361 354 237 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1012 1663 2868 3001 3460 3669 1012
Size: 651 1205 133 459 209 2628
Fragments arranged by size:
2628 1205 651 459 209 133
TatI w'GTAC_w
Cuts at: 1664 4800 1664
Size: 3136 2149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1423 1455 4802 4834 1423
Size: 32 3347 32 1874
Fragments arranged by size:
3347 1874 32 32
Tth111I GACn'n_nGTC
Cuts at: 3082 3082
Size: 5285
XbaI T'CTAG_A
Cuts at: 2316 2316
Size: 5285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1006 1725 1980 2780 1006
Size: 719 255 800 3511
Fragments arranged by size:
3511 800 719 255
XhoI C'TCGA_G
Cuts at: 2239 2239
Size: 5285
XmaI C'CCGG_G
Cuts at: 2305 2305
Size: 5285
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BfrBI BmgBI BmtI BsiWI BspEI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI