Restriction map for
pF1KB5443
(Circular) (Six-base) MAPSORT of: pF1KB5443.seq Check: 2623 from: 1 to: 3692
pF1KB5443 3692 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 470 730 470
Size: 260 3432
Acc65I G'GTAC_C
Cuts at: 708 708
Size: 3692
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1517 1949 2696 2964 1517
Size: 432 747 268 2245
Fragments arranged by size:
2245 747 432 268
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 997 2564 997
Size: 1567 2125
ApaI G_GGCC'C
Cuts at: 160 160
Size: 3692
ApaLI G'TGCA_C
Cuts at: 2462 2462
Size: 3692
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 554 712 554
Size: 158 3534
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 708 1122 1370 1405 708
Size: 414 248 35 2995
Fragments arranged by size:
2995 414 248 35
BanII G_rGCy'C
Cuts at: 160 188 706 1736 160
Size: 28 518 1030 2116
Fragments arranged by size:
2116 1030 518 28
BbeI G_GCGC'C
Cuts at: 1126 1374 1126
Size: 248 3444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 402 436 90
Size: 34 278 34 3346
Fragments arranged by size:
3346 278 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 353 1212 353
Size: 859 2833
BglI GCCn_nnn'nGGC
Cuts at: 292 292
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 160 253 1317 1410 2466 160
Size: 93 1064 93 1056 1386
Fragments arranged by size:
1386 1064 1056 93 93
BmgBI CAC'GTC
Cuts at: 236 549 236
Size: 313 3379
BmrI ACTGGGnnnn_n'
Cuts at: 904 985 1320 3464 904
Size: 81 335 2144 1132
Fragments arranged by size:
2144 1132 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 628 2059 628
Size: 1431 2261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 217 640 142
Size: 75 423 3194
Fragments arranged by size:
3194 423 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 868 1747 2239 2537 598
Size: 270 879 492 298 1753
Fragments arranged by size:
1753 879 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 459 1045 459
Size: 586 3106
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 116 1123 1371 2073 3569 116
Size: 1007 248 702 1496 239
Fragments arranged by size:
1496 1007 702 248 239
BsaWI w'CCGG_w
Cuts at: 664 881 1402 2080 2354 2501 2912 664
Size: 217 521 678 274 147 411 1444
Fragments arranged by size:
1444 678 521 411 274 217 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 190
Size: 30 3662
BseYI C'CCAG_C
Cuts at: 111 135 1117 1875 2452 2948 111
Size: 24 982 758 577 496 855
Fragments arranged by size:
982 855 758 577 496 24
BsiEI CG_ry'CG
Cuts at: 104 603 1234 1280 2488 104
Size: 499 631 46 1208 1308
Fragments arranged by size:
1308 1208 631 499 46
BsiHKAI G_wGCw'C
Cuts at: 316 706 1484 1674 2466 316
Size: 390 778 190 792 1542
Fragments arranged by size:
1542 792 778 390 190
BsmBI CGTCTCn'nnnn_
Cuts at: 172 172
Size: 3692
Bsp1286I G_dGCh'C
Cuts at: 160 188 253 316 706 1317 1410 1484 1674
Size: 28 65 63 390 611 93 74 190
Cuts at: 1674 1736 2466 160
Size: 62 730 1386
Fragments arranged by size:
1386 730 611 390 190 93 74 65
63 62 28
BspEI T'CCGG_A
Cuts at: 664 2912 664
Size: 2248 1444
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 742 956 1258 1639 742
Size: 214 302 381 2795
Fragments arranged by size:
2795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 624 1984 2038 3522 3687 50
Size: 574 1360 54 1484 165 55
Fragments arranged by size:
1484 1360 574 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 516 1690 1871 2080 516
Size: 1174 181 209 2128
Fragments arranged by size:
2128 1174 209 181
BssSI C'ACGA_G
Cuts at: 322 1963 2321 322
Size: 1641 358 1693
Fragments arranged by size:
1693 1641 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstEII G'GTnAC_C
Cuts at: 538 538
Size: 3692
BstXI CCAn_nnnn'nTGG
Cuts at: 195 195
Size: 3692
BstYI r'GATC_y
Cuts at: 1 437 717 1184 1207 1542 1788 2800 2886
Size: 436 280 467 23 335 246 1012 86
Cuts at: 2886 2898 3244 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 436 346 335 280 246
86 23 12
BtgI C'CryG_G
Cuts at: 165 245 165
Size: 80 3612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 405 1642 1823 1986 405
Size: 1237 181 163 2111
Fragments arranged by size:
2111 1237 181 163
BtsI GCAGTG_nn'
Cuts at: 251 935 251
Size: 684 3008
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 1398 2256 1398
Size: 858 2834
EaeI y'GGCC_r
Cuts at: 514 1277 1451 1842 1869 3214 514
Size: 763 174 391 27 1345 992
Fragments arranged by size:
1345 992 763 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 217 640 1517 1949 2696 2964 142
Size: 75 423 877 432 747 268 870
Fragments arranged by size:
877 870 747 432 423 268 75
EcoO109I rG'GnC_Cy
Cuts at: 831 831
Size: 3692
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 287 1126 1374 2396 3375 287
Size: 839 248 1022 979 604
Fragments arranged by size:
1022 979 839 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 566 598 1534 1564 1566 1596 327
Size: 32 207 32 936 30 2 30 2423
Fragments arranged by size:
2423 936 207 32 32 30 30 2
HincII GTy'rAC
Cuts at: 731 731
Size: 3692
KasI G'GCGC_C
Cuts at: 1122 1370 1122
Size: 248 3444
KpnI G_GTAC'C
Cuts at: 712 712
Size: 3692
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 251 263 2363 2547 251
Size: 12 2100 184 1396
Fragments arranged by size:
2100 1396 184 12
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 268 490 1681 1808 268
Size: 222 1191 127 2152
Fragments arranged by size:
2152 1191 222 127
MspA1I CmG'CkG
Cuts at: 167 803 1117 1477 2490 2735 167
Size: 636 314 360 1013 245 1124
Fragments arranged by size:
1124 1013 636 360 314 245
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 1123 1371 1123
Size: 248 3444
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 745 1245 2152 745
Size: 500 907 2285
Fragments arranged by size:
2285 907 500
PasI CC'CwG_GG
Cuts at: 258 258
Size: 3692
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PfoI T'CCnGG_A
Cuts at: 119 119
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PmlI CAC'GTG
Cuts at: 459 459
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PspOMI G'GGCC_C
Cuts at: 156 156
Size: 3692
PstI C_TGCA'G
Cuts at: 739 1424 739
Size: 685 3007
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 1117 1477 1117
Size: 360 3332
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SacII CC_GC'GG
Cuts at: 168 168
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 739 739
Size: 3692
ScaI AGT'ACT
Cuts at: 652 3209 652
Size: 2557 1135
SexAI A'CCwGG_T
Cuts at: 368 368
Size: 3692
SfcI C'TryA_G
Cuts at: 33 441 735 1420 2413 2604 33
Size: 408 294 685 993 191 1121
Fragments arranged by size:
1121 993 685 408 294 191
SfoI GGC'GCC
Cuts at: 1124 1372 1124
Size: 248 3444
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 556 714 556
Size: 158 3534
SmlI C'TyrA_G
Cuts at: 613 847 1762 2254 2516 613
Size: 234 915 492 262 1789
Fragments arranged by size:
1789 915 492 262 234
SpeI A'CTAG_T
Cuts at: 67 375 67
Size: 308 3384
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StyI C'CwwG_G
Cuts at: 287 826 1180 287
Size: 539 354 2799
Fragments arranged by size:
2799 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 1275 1408 1867 2076 617
Size: 658 133 459 209 2233
Fragments arranged by size:
2233 658 459 209 133
TatI w'GTAC_w
Cuts at: 650 3207 650
Size: 2557 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 190 222 313 345 659 691 3209 3241 190
Size: 32 91 32 314 32 2518 32 641
Fragments arranged by size:
2518 641 314 91 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 554 712 554
Size: 158 3534
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BseRI
BsgI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PpuMI PshAI PsiI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI