Restriction map for
pF1KB5441
(Circular) (Six-base) MAPSORT of: pF1KB5441.seq Check: 2512 from: 1 to: 4130
pF1KB5441 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 286 1146 286
Size: 860 3270
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 596 1955 2387 2740 3402 361
Size: 235 1359 432 353 662 1089
Fragments arranged by size:
1359 1089 662 432 353 235
AflIII A'CryG_T
Cuts at: 912 1482 3282 3563 912
Size: 570 1800 281 1479
Fragments arranged by size:
1800 1479 570 281
AlwNI CAG_nnn'CTG
Cuts at: 1435 2873 1435
Size: 1438 2692
ApaI G_GGCC'C
Cuts at: 305 305
Size: 4130
ApaLI G'TGCA_C
Cuts at: 2968 2968
Size: 4130
ApoI r'AATT_y
Cuts at: 1091 1134 3483 3739 1091
Size: 43 2349 256 1482
Fragments arranged by size:
2349 1482 256 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 1150 1150
Size: 4130
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 286 1146 1560 1808 1843 286
Size: 860 414 248 35 2573
Fragments arranged by size:
2573 860 414 248 35
BanII G_rGCy'C
Cuts at: 305 418 1144 2174 305
Size: 113 726 1030 2261
Fragments arranged by size:
2261 1030 726 113
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BciVI GTATCCnnnnn_n'
Cuts at: 778 2028 3084 4130 778
Size: 1250 1056 1046 778
Fragments arranged by size:
1250 1056 1046 778
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BglI GCCn_nnn'nGGC
Cuts at: 442 442
Size: 4130
BglII A'GATC_T
Cuts at: 734 833 1645 3682 734
Size: 99 812 2037 1182
Fragments arranged by size:
2037 1182 812 99
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 242 305 1755 1848 2972 242
Size: 63 1450 93 1124 1400
Fragments arranged by size:
1450 1400 1124 93 63
BmgBI CAC'GTC
Cuts at: 492 678 915 492
Size: 186 237 3707
Fragments arranged by size:
3707 237 186
BmrI ACTGGGnnnn_n'
Cuts at: 1026 1122 1342 1423 1758 3902 1026
Size: 96 220 81 335 2144 1254
Fragments arranged by size:
2144 1254 335 220 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2185 2899 3197 1306
Size: 879 714 298 2239
Fragments arranged by size:
2239 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 401 3834 401
Size: 3433 697
BsaAI yAC'GTr
Cuts at: 227 1483 227
Size: 1256 2874
BsaBI GATnn'nnATC
Cuts at: 6 472 1644 3681 6
Size: 466 1172 2037 455
Fragments arranged by size:
2037 1172 466 455
BsaHI Gr'CG_yC
Cuts at: 1561 1809 2511 4007 1561
Size: 248 702 1496 1684
Fragments arranged by size:
1684 1496 702 248
BsaWI w'CCGG_w
Cuts at: 464 919 1319 1840 2518 2929 3076 3350 464
Size: 455 400 521 678 411 147 274 1244
Fragments arranged by size:
1244 678 521 455 411 400 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 400 430 787 817 400
Size: 30 357 30 3713
Fragments arranged by size:
3713 357 30 30
BseYI C'CCAG_C
Cuts at: 431 959 1555 2313 2978 3386 431
Size: 528 596 758 665 408 1175
Fragments arranged by size:
1175 758 665 596 528 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 663
Size: 4130
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2948 104
Size: 1568 46 1230 1286
Fragments arranged by size:
1568 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 674 1144 1922 2112 2972 674
Size: 470 778 190 860 1832
Fragments arranged by size:
1832 860 778 470 190
BsmBI CGTCTCn'nnnn_
Cuts at: 807 807
Size: 4130
Bsp1286I G_dGCh'C
Cuts at: 242 305 418 674 1144 1755 1848 1922 2112
Size: 63 113 256 470 611 93 74 190
Cuts at: 2112 2174 2972 242
Size: 62 798 1400
Fragments arranged by size:
1400 798 611 470 256 190 113 93
74 63 62
BspHI T'CATG_A
Cuts at: 2562 2562
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 527 1180 1394 1696 2077 527
Size: 653 214 302 381 2580
Fragments arranged by size:
2580 653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2422 2476 3960 4125 50
Size: 2372 54 1484 165 55
Fragments arranged by size:
2372 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2042 2042
Size: 4130
BsrFI r'CCGG_y
Cuts at: 2128 2309 2128
Size: 181 3949
BssSI C'ACGA_G
Cuts at: 2401 3109 2401
Size: 708 3422
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 467 734 833 1155 1622 1645 1980 2226
Size: 466 267 99 322 467 23 335 246
Cuts at: 2226 2532 2544 2630 3682 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 467 466 449 335 322 306 267
246 99 86 23 12
BtgI C'CryG_G
Cuts at: 851 851
Size: 4130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 1373 1373
Size: 4130
DraI TTT'AAA
Cuts at: 1038 1129 1038
Size: 91 4039
DrdI GACnn_nn'nnGTC
Cuts at: 1836 3180 1836
Size: 1344 2786
EaeI y'GGCC_r
Cuts at: 149 376 827 936 1715 1889 2280 2307 3652
Size: 227 451 109 779 174 391 27 1345
Cuts at: 3652 149
Size: 627
Fragments arranged by size:
1345 779 627 451 391 227 174 109
27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 268 2153 2363 268
Size: 1885 210 2035
Fragments arranged by size:
2035 1885 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 526 2338 3080 3226 3940 526
Size: 1812 742 146 714 716
Fragments arranged by size:
1812 742 716 714 146
EcoICRI GAG'CTC
Cuts at: 1142 1142
Size: 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 596 1955 2387 2740 3402 361
Size: 235 1359 432 353 662 1089
Fragments arranged by size:
1359 1089 662 432 353 235
EcoO109I rG'GnC_Cy
Cuts at: 167 336 516 1269 167
Size: 169 180 753 3028
Fragments arranged by size:
3028 753 180 169
EcoRI G'AATT_C
Cuts at: 1091 1134 1091
Size: 43 4087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 4130
FspI TGC'GCA
Cuts at: 488 1911 488
Size: 1423 2707
FspAI rTGC'GCAy
Cuts at: 488 488
Size: 4130
HaeII r_GCGC'y
Cuts at: 1564 1812 3042 3813 1564
Size: 248 1230 771 1881
Fragments arranged by size:
1881 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 1972 2002 2004 2034 400
Size: 32 1540 30 2 30 2496
Fragments arranged by size:
2496 1540 32 30 30 2
HincII GTy'rAC
Cuts at: 201 210 681 1169 201
Size: 9 471 488 3162
Fragments arranged by size:
3162 488 471 9
HindIII A'AGCT_T
Cuts at: 354 578 354
Size: 224 3906
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 290 1150 290
Size: 860 3270
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 2889 3073 167
Size: 2722 184 1224
Fragments arranged by size:
2722 1224 184
MscI TGG'CCA
Cuts at: 151 378 938 1891 151
Size: 227 560 953 2390
Fragments arranged by size:
2390 953 560 227
MslI CAynn'nnrTG
Cuts at: 482 2119 2246 482
Size: 1637 127 2366
Fragments arranged by size:
2366 1637 127
MspA1I CmG'CkG
Cuts at: 1241 1555 1915 2699 2944 1241
Size: 314 360 784 245 2427
Fragments arranged by size:
2427 784 360 314 245
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NspI r_CATG'y
Cuts at: 530 543 1183 1683 3286 530
Size: 13 640 500 1603 1374
Fragments arranged by size:
1603 1374 640 500 13
PciI A'CATG_T
Cuts at: 3282 3282
Size: 4130
PflMI CCAn_nnn'nTGG
Cuts at: 1004 1004
Size: 4130
PfoI T'CCnGG_A
Cuts at: 1003 1003
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PmlI CAC'GTG
Cuts at: 227 227
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 4098
PpuMI rG'GwC_Cy
Cuts at: 336 516 336
Size: 180 3950
PspOMI G'GGCC_C
Cuts at: 301 301
Size: 4130
PstI C_TGCA'G
Cuts at: 1177 1862 1177
Size: 685 3445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 1555 1915 1555
Size: 360 3770
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 1144 1144
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 1111 3647 1111
Size: 2536 1594
SfcI C'TryA_G
Cuts at: 33 750 795 1173 1858 2826 3017 33
Size: 717 45 378 685 968 191 1146
Fragments arranged by size:
1146 968 717 685 378 191 45
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 1285 2200 2914 3176 1285
Size: 915 714 262 2239
Fragments arranged by size:
2239 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
StuI AGG'CCT
Cuts at: 605 605
Size: 4130
StyI C'CwwG_G
Cuts at: 1264 1618 1264
Size: 354 3776
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 286 922 1713 1846 2305 2514 286
Size: 636 791 133 459 209 1902
Fragments arranged by size:
1902 791 636 459 209 133
TatI w'GTAC_w
Cuts at: 1109 3645 1109
Size: 2536 1594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 418 951 1625 418
Size: 533 674 2923
Fragments arranged by size:
2923 674 533
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI