Restriction map for
pF1KB5430
(Circular) (Six-base) MAPSORT of: pF1KB5430.seq Check: 3477 from: 1 to: 3566
pF1KB5430 3566 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 604 604
Size: 3566
Acc65I G'GTAC_C
Cuts at: 582 582
Size: 3566
AclI AA'CG_TT
Cuts at: 3406 3406
Size: 3566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 1391 1823 2570 2838 194
Size: 1197 432 747 268 922
Fragments arranged by size:
1197 922 747 432 268
AflIII A'CryG_T
Cuts at: 918 2022 2999 918
Size: 1104 977 1485
Fragments arranged by size:
1485 1104 977
AgeI A'CCGG_T
Cuts at: 1954 1954
Size: 3566
AlwNI CAG_nnn'CTG
Cuts at: 871 2438 871
Size: 1567 1999
ApaI G_GGCC'C
Cuts at: 444 444
Size: 3566
ApaLI G'TGCA_C
Cuts at: 2336 2336
Size: 3566
ApoI r'AATT_y
Cuts at: 570 2919 3175 570
Size: 2349 256 961
Fragments arranged by size:
2349 961 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
AvaI C'yCGr_G
Cuts at: 471 586 471
Size: 115 3451
BamHI G'GATC_C
Cuts at: 591 591
Size: 3566
BanI G'GyrC_C
Cuts at: 111 582 996 1244 1279 111
Size: 471 414 248 35 2398
Fragments arranged by size:
2398 471 414 248 35
BanII G_rGCy'C
Cuts at: 444 462 580 1610 444
Size: 18 118 1030 2400
Fragments arranged by size:
2400 1030 118 18
BbeI G_GCGC'C
Cuts at: 1000 1248 1000
Size: 248 3318
BciVI GTATCCnnnnn_n'
Cuts at: 1464 2225 3566 1464
Size: 761 1341 1464
Fragments arranged by size:
1464 1341 761
BclI T'GATC_A
Cuts at: 1086 1086
Size: 3566
BglI GCCn_nnn'nGGC
Cuts at: 448 448
Size: 3566
BglII A'GATC_T
Cuts at: 1081 3118 1081
Size: 2037 1529
BlpI GC'TnA_GC
Cuts at: 678 678
Size: 3566
Bme1580I G_kGCm'C
Cuts at: 444 1191 1284 2340 444
Size: 747 93 1056 1670
Fragments arranged by size:
1670 1056 747 93
BmrI ACTGGGnnnn_n'
Cuts at: 438 778 859 1194 3338 438
Size: 340 81 335 2144 666
Fragments arranged by size:
2144 666 340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1621 2113 2411 742
Size: 879 492 298 1897
Fragments arranged by size:
1897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3270 3270
Size: 3566
BsaAI yAC'GTr
Cuts at: 919 919
Size: 3566
BsaBI GATnn'nnATC
Cuts at: 6 1080 3117 6
Size: 1074 2037 455
Fragments arranged by size:
2037 1074 455
BsaHI Gr'CG_yC
Cuts at: 997 1245 1947 3443 997
Size: 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 248
BsaWI w'CCGG_w
Cuts at: 755 1276 1954 2228 2375 2786 755
Size: 521 678 274 147 411 1535
Fragments arranged by size:
1535 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 170 200 170
Size: 30 3536
BseYI C'CCAG_C
Cuts at: 369 991 1749 2326 2822 369
Size: 622 758 577 496 1113
Fragments arranged by size:
1113 758 622 577 496
BsiEI CG_ry'CG
Cuts at: 104 1108 1154 2362 104
Size: 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 46
BsiHKAI G_wGCw'C
Cuts at: 580 1358 1548 2340 580
Size: 778 190 792 1806
Fragments arranged by size:
1806 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 444 462 580 1191 1284 1358 1548 1610 2340
Size: 18 118 611 93 74 190 62 730
Cuts at: 2340 444
Size: 1670
Fragments arranged by size:
1670 730 611 190 118 93 74 62
18
BspEI T'CCGG_A
Cuts at: 2786 2786
Size: 3566
BspHI T'CATG_A
Cuts at: 2742 2742
Size: 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 830 1132 1513 616
Size: 214 302 381 2669
Fragments arranged by size:
2669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 470 1858 1912 3396 3561 50
Size: 420 1388 54 1484 165 55
Fragments arranged by size:
1484 1388 420 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1478 1478
Size: 3566
BsrFI r'CCGG_y
Cuts at: 1564 1745 1954 1564
Size: 181 209 3176
Fragments arranged by size:
3176 209 181
BssSI C'ACGA_G
Cuts at: 1837 2195 1837
Size: 358 3208
BstAPI GCAn_nnn'nTGC
Cuts at: 943 943
Size: 3566
BstBI TT'CG_AA
Cuts at: 1927 1927
Size: 3566
BstYI r'GATC_y
Cuts at: 1 591 1058 1081 1416 1662 2674 2760 2772
Size: 590 467 23 335 246 1012 86 12
Cuts at: 2772 3118 1
Size: 346 449
Fragments arranged by size:
1012 590 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 164 164
Size: 3566
BtgI C'CryG_G
Cuts at: 107 546 107
Size: 439 3127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1516 1697 1860 1516
Size: 181 163 3222
Fragments arranged by size:
3222 181 163
BtsI GCAGTG_nn'
Cuts at: 809 809
Size: 3566
DraI TTT'AAA
Cuts at: 565 565
Size: 3566
DrdI GACnn_nn'nnGTC
Cuts at: 229 1272 2130 229
Size: 1043 858 1665
Fragments arranged by size:
1665 1043 858
EaeI y'GGCC_r
Cuts at: 1151 1325 1716 1743 3088 1151
Size: 174 391 27 1345 1629
Fragments arranged by size:
1629 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1151 1151
Size: 3566
EarI CTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 504 1774 2084 2230 3376 504
Size: 1270 310 146 1146 694
Fragments arranged by size:
1270 1146 694 310 146
EcoICRI GAG'CTC
Cuts at: 578 578
Size: 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 1391 1823 2570 2838 194
Size: 1197 432 747 268 922
Fragments arranged by size:
1197 922 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 440 705 440
Size: 265 3301
EcoRI G'AATT_C
Cuts at: 570 570
Size: 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1585 1585
Size: 3566
FspI TGC'GCA
Cuts at: 1347 1347
Size: 3566
HaeII r_GCGC'y
Cuts at: 1000 1248 2270 3249 1000
Size: 248 1022 979 1317
Fragments arranged by size:
1317 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1408 1438 1440 1470 298
Size: 32 1078 30 2 30 2394
Fragments arranged by size:
2394 1078 32 30 30 2
HincII GTy'rAC
Cuts at: 605 605
Size: 3566
KasI G'GCGC_C
Cuts at: 996 1244 996
Size: 248 3318
KpnI G_GTAC'C
Cuts at: 586 586
Size: 3566
MluI A'CGCG_T
Cuts at: 2999 2999
Size: 3566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2421 2237
Size: 184 3382
MscI TGG'CCA
Cuts at: 1327 1327
Size: 3566
MslI CAynn'nnrTG
Cuts at: 1555 1682 1555
Size: 127 3439
MspA1I CmG'CkG
Cuts at: 677 991 1351 2364 2609 677
Size: 314 360 1013 245 1634
Fragments arranged by size:
1634 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1747 1747
Size: 3566
NarI GG'CG_CC
Cuts at: 997 1245 997
Size: 248 3318
NcoI C'CATG_G
Cuts at: 107 107
Size: 3566
NdeI CA'TA_TG
Cuts at: 321 321
Size: 3566
NgoMIV G'CCGG_C
Cuts at: 1745 1745
Size: 3566
NspI r_CATG'y
Cuts at: 619 1119 2026 619
Size: 500 907 2159
Fragments arranged by size:
2159 907 500
PciI A'CATG_T
Cuts at: 2022 2022
Size: 3566
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 3566
PmeI GTTT'AAAC
Cuts at: 565 565
Size: 3566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 3534
PspOMI G'GGCC_C
Cuts at: 440 440
Size: 3566
PstI C_TGCA'G
Cuts at: 613 1298 613
Size: 685 2881
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3566
PvuII CAG'CTG
Cuts at: 991 1351 991
Size: 360 3206
RsrII CG'GwC_CG
Cuts at: 1761 1761
Size: 3566
SacI G_AGCT'C
Cuts at: 580 580
Size: 3566
SalI G'TCGA_C
Cuts at: 603 603
Size: 3566
SapI GCTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
SbfI CC_TGCA'GG
Cuts at: 613 613
Size: 3566
ScaI AGT'ACT
Cuts at: 3083 3083
Size: 3566
SfcI C'TryA_G
Cuts at: 33 609 1294 2287 2478 33
Size: 576 685 993 191 1121
Fragments arranged by size:
1121 993 685 576 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 448 448
Size: 3566
SfoI GGC'GCC
Cuts at: 998 1246 998
Size: 248 3318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
SmaI CCC'GGG
Cuts at: 588 588
Size: 3566
SmlI C'TyrA_G
Cuts at: 721 1636 2128 2390 721
Size: 915 492 262 1897
Fragments arranged by size:
1897 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3566
SphI G_CATG'C
Cuts at: 619 1119 619
Size: 500 3066
StyI C'CwwG_G
Cuts at: 107 700 1054 107
Size: 593 354 2619
Fragments arranged by size:
2619 593 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 317 1149 1282 1741 1950 317
Size: 832 133 459 209 1933
Fragments arranged by size:
1933 832 459 209 133
TatI w'GTAC_w
Cuts at: 3081 3081
Size: 3566
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 152 184 3083 3115 152
Size: 32 2899 32 603
Fragments arranged by size:
2899 603 32 32
Tth111I GACn'n_nGTC
Cuts at: 1363 1363
Size: 3566
XbaI T'CTAG_A
Cuts at: 597 597
Size: 3566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 1061
Size: 3566
XmaI C'CCGG_G
Cuts at: 586 586
Size: 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI