Restriction map for
pF1KB5425
(Circular) (Six-base) MAPSORT of: pF1KB5425.seq Check: 4702 from: 1 to: 3503
pF1KB5425 3503 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 541 541
Size: 3503
Acc65I G'GTAC_C
Cuts at: 519 519
Size: 3503
AclI AA'CG_TT
Cuts at: 3343 3343
Size: 3503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1328 1760 2507 2775 388
Size: 940 432 747 268 1116
Fragments arranged by size:
1116 940 747 432 268
AflIII A'CryG_T
Cuts at: 855 1959 2936 855
Size: 1104 977 1422
Fragments arranged by size:
1422 1104 977
AgeI A'CCGG_T
Cuts at: 1891 1891
Size: 3503
AleI CACnn'nnGTG
Cuts at: 340 340
Size: 3503
AlwNI CAG_nnn'CTG
Cuts at: 239 808 2375 239
Size: 569 1567 1367
Fragments arranged by size:
1567 1367 569
ApaLI G'TGCA_C
Cuts at: 2273 2273
Size: 3503
ApoI r'AATT_y
Cuts at: 507 2856 3112 507
Size: 2349 256 898
Fragments arranged by size:
2349 898 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
AvaI C'yCGr_G
Cuts at: 523 523
Size: 3503
BamHI G'GATC_C
Cuts at: 528 528
Size: 3503
BanI G'GyrC_C
Cuts at: 519 933 1181 1216 519
Size: 414 248 35 2806
Fragments arranged by size:
2806 414 248 35
BanII G_rGCy'C
Cuts at: 517 1547 517
Size: 1030 2473
BbeI G_GCGC'C
Cuts at: 937 1185 937
Size: 248 3255
BbsI GAAGACnn'nnnn_
Cuts at: 200 200
Size: 3503
BciVI GTATCCnnnnn_n'
Cuts at: 421 1401 2162 3503 421
Size: 980 761 1341 421
Fragments arranged by size:
1341 980 761 421
BclI T'GATC_A
Cuts at: 1023 1023
Size: 3503
BglII A'GATC_T
Cuts at: 162 311 371 1018 3055 162
Size: 149 60 647 2037 610
Fragments arranged by size:
2037 647 610 149 60
BlpI GC'TnA_GC
Cuts at: 615 615
Size: 3503
Bme1580I G_kGCm'C
Cuts at: 1128 1221 2277 1128
Size: 93 1056 2354
Fragments arranged by size:
2354 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 410 715 796 1131 3275 410
Size: 305 81 335 2144 638
Fragments arranged by size:
2144 638 335 305 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 3503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 679 1558 2050 2348 160
Size: 519 879 492 298 1315
Fragments arranged by size:
1315 879 519 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3207 3207
Size: 3503
BsaAI yAC'GTr
Cuts at: 856 856
Size: 3503
BsaBI GATnn'nnATC
Cuts at: 6 1017 3054 6
Size: 1011 2037 455
Fragments arranged by size:
2037 1011 455
BsaHI Gr'CG_yC
Cuts at: 934 1182 1884 3380 934
Size: 248 702 1496 1057
Fragments arranged by size:
1496 1057 702 248
BsaWI w'CCGG_w
Cuts at: 692 1213 1891 2165 2312 2723 692
Size: 521 678 274 147 411 1472
Fragments arranged by size:
1472 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 271 301 271
Size: 30 3473
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 394 232
Size: 162 3341
BseYI C'CCAG_C
Cuts at: 202 928 1686 2263 2759 202
Size: 726 758 577 496 946
Fragments arranged by size:
946 758 726 577 496
BsiEI CG_ry'CG
Cuts at: 104 1045 1091 2299 104
Size: 941 46 1208 1308
Fragments arranged by size:
1308 1208 941 46
BsiHKAI G_wGCw'C
Cuts at: 302 517 1295 1485 2277 302
Size: 215 778 190 792 1528
Fragments arranged by size:
1528 792 778 215 190
Bsp1286I G_dGCh'C
Cuts at: 302 517 1128 1221 1295 1485 1547 2277 302
Size: 215 611 93 74 190 62 730 1528
Fragments arranged by size:
1528 730 611 215 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2723 2723
Size: 3503
BspHI T'CATG_A
Cuts at: 2679 2679
Size: 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 553 767 1069 1450 553
Size: 214 302 381 2606
Fragments arranged by size:
2606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1795 1849 3333 3498 50
Size: 1745 54 1484 165 55
Fragments arranged by size:
1745 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 1415
Size: 3503
BsrFI r'CCGG_y
Cuts at: 1501 1682 1891 1501
Size: 181 209 3113
Fragments arranged by size:
3113 209 181
BssSI C'ACGA_G
Cuts at: 1774 2132 1774
Size: 358 3145
BstAPI GCAn_nnn'nTGC
Cuts at: 880 880
Size: 3503
BstBI TT'CG_AA
Cuts at: 1864 1864
Size: 3503
BstYI r'GATC_y
Cuts at: 1 162 311 371 528 995 1018 1353 1599
Size: 161 149 60 157 467 23 335 246
Cuts at: 1599 2611 2697 2709 3055 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 161 157
149 86 60 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1453 1634 1797 1453
Size: 181 163 3159
Fragments arranged by size:
3159 181 163
BtsI GCAGTG_nn'
Cuts at: 240 746 240
Size: 506 2997
DraI TTT'AAA
Cuts at: 502 502
Size: 3503
DrdI GACnn_nn'nnGTC
Cuts at: 1209 2067 1209
Size: 858 2645
EaeI y'GGCC_r
Cuts at: 1088 1262 1653 1680 3025 1088
Size: 174 391 27 1345 1566
Fragments arranged by size:
1566 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1088 1088
Size: 3503
EarI CTCTTCn'nnn_
Cuts at: 455 1526 1736 455
Size: 1071 210 2222
Fragments arranged by size:
2222 1071 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1711 2021 2167 3313 1711
Size: 310 146 1146 1901
Fragments arranged by size:
1901 1146 310 146
EcoICRI GAG'CTC
Cuts at: 515 515
Size: 3503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 388 1328 1760 2507 2775 274
Size: 114 940 432 747 268 1002
Fragments arranged by size:
1002 940 747 432 268 114
EcoO109I rG'GnC_Cy
Cuts at: 122 642 122
Size: 520 2983
EcoRI G'AATT_C
Cuts at: 507 507
Size: 3503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1522 1522
Size: 3503
FspI TGC'GCA
Cuts at: 1284 1284
Size: 3503
HaeII r_GCGC'y
Cuts at: 937 1185 2207 3186 937
Size: 248 1022 979 1254
Fragments arranged by size:
1254 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 1345 1375 1377 1407 271
Size: 32 1042 30 2 30 2367
Fragments arranged by size:
2367 1042 32 30 30 2
HincII GTy'rAC
Cuts at: 348 542 348
Size: 194 3309
KasI G'GCGC_C
Cuts at: 933 1181 933
Size: 248 3255
KpnI G_GTAC'C
Cuts at: 523 523
Size: 3503
MluI A'CGCG_T
Cuts at: 2936 2936
Size: 3503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2358 2174
Size: 184 3319
MscI TGG'CCA
Cuts at: 1264 1264
Size: 3503
MslI CAynn'nnrTG
Cuts at: 340 1492 1619 340
Size: 1152 127 2224
Fragments arranged by size:
2224 1152 127
MspA1I CmG'CkG
Cuts at: 614 928 1288 2301 2546 614
Size: 314 360 1013 245 1571
Fragments arranged by size:
1571 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1684 1684
Size: 3503
NarI GG'CG_CC
Cuts at: 934 1182 934
Size: 248 3255
NcoI C'CATG_G
Cuts at: 107 107
Size: 3503
NgoMIV G'CCGG_C
Cuts at: 1682 1682
Size: 3503
NspI r_CATG'y
Cuts at: 556 1056 1963 556
Size: 500 907 2096
Fragments arranged by size:
2096 907 500
PciI A'CATG_T
Cuts at: 1959 1959
Size: 3503
PfoI T'CCnGG_A
Cuts at: 251 251
Size: 3503
PmeI GTTT'AAAC
Cuts at: 502 502
Size: 3503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 3471
PpuMI rG'GwC_Cy
Cuts at: 122 122
Size: 3503
PstI C_TGCA'G
Cuts at: 550 1235 550
Size: 685 2818
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3503
PvuII CAG'CTG
Cuts at: 928 1288 928
Size: 360 3143
RsrII CG'GwC_CG
Cuts at: 1698 1698
Size: 3503
SacI G_AGCT'C
Cuts at: 517 517
Size: 3503
SalI G'TCGA_C
Cuts at: 540 540
Size: 3503
SanDI GG'GwC_CC
Cuts at: 122 122
Size: 3503
SapI GCTCTTCn'nnn_
Cuts at: 1526 1736 1526
Size: 210 3293
SbfI CC_TGCA'GG
Cuts at: 550 550
Size: 3503
ScaI AGT'ACT
Cuts at: 3020 3020
Size: 3503
SfcI C'TryA_G
Cuts at: 33 351 546 1231 2224 2415 33
Size: 318 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 318 195 191
SfoI GGC'GCC
Cuts at: 935 1183 935
Size: 248 3255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
SmaI CCC'GGG
Cuts at: 525 525
Size: 3503
SmlI C'TyrA_G
Cuts at: 175 658 1573 2065 2327 175
Size: 483 915 492 262 1351
Fragments arranged by size:
1351 915 492 483 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3503
SphI G_CATG'C
Cuts at: 556 1056 556
Size: 500 3003
StyI C'CwwG_G
Cuts at: 107 637 991 107
Size: 530 354 2619
Fragments arranged by size:
2619 530 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1086 1219 1678 1887 1086
Size: 133 459 209 2702
Fragments arranged by size:
2702 459 209 133
TatI w'GTAC_w
Cuts at: 428 3018 428
Size: 2590 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3471
Tth111I GACn'n_nGTC
Cuts at: 1300 1300
Size: 3503
XbaI T'CTAG_A
Cuts at: 165 534 165
Size: 369 3134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 998 998
Size: 3503
XmaI C'CCGG_G
Cuts at: 523 523
Size: 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI