Restriction map for
pF1KB5422
(Circular) (Six-base) MAPSORT of: pF1KB5422.seq Check: 7582 from: 1 to: 3785
pF1KB5422 3785 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1610 2042 2789 3057 389
Size: 1221 432 747 268 1117
Fragments arranged by size:
1221 1117 747 432 268
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AleI CACnn'nnGTG
Cuts at: 166 166
Size: 3785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 277 277
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 186 215 523 1090 2657 186
Size: 29 308 567 1567 1314
Fragments arranged by size:
1567 1314 567 308 29
ApaI G_GGCC'C
Cuts at: 738 738
Size: 3785
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 454 789 3138 3394 454
Size: 335 2349 256 845
Fragments arranged by size:
2349 845 335 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 136 437 703 805 136
Size: 301 266 102 3116
Fragments arranged by size:
3116 301 266 102
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 801 1215 1463 1498 801
Size: 414 248 35 3088
Fragments arranged by size:
3088 414 248 35
BanII G_rGCy'C
Cuts at: 738 764 799 1829 738
Size: 26 35 1030 2694
Fragments arranged by size:
2694 1030 35 26
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 712 746 712
Size: 34 3751
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglII A'GATC_T
Cuts at: 314 1300 3337 314
Size: 986 2037 762
Fragments arranged by size:
2037 986 762
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 173 738 1410 1503 2559 173
Size: 565 672 93 1056 1399
Fragments arranged by size:
1399 1056 672 565 93
BmrI ACTGGGnnnn_n'
Cuts at: 297 694 997 1078 1413 3557 297
Size: 397 303 81 335 2144 525
Fragments arranged by size:
2144 525 397 335 303 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 450 2152 450
Size: 1702 2083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 408 172
Size: 236 3549
Bpu10I CC'TnA_GC
Cuts at: 758 758
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1840 2332 2630 961
Size: 879 492 298 2116
Fragments arranged by size:
2116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 434 613 3489 434
Size: 179 2876 730
Fragments arranged by size:
2876 730 179
BsaAI yAC'GTr
Cuts at: 168 1138 168
Size: 970 2815
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 639 1216 1464 2166 3662 639
Size: 577 248 702 1496 762
Fragments arranged by size:
1496 762 702 577 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 605 635 605
Size: 30 3755
BseYI C'CCAG_C
Cuts at: 1210 1968 2545 3041 1210
Size: 758 577 496 1954
Fragments arranged by size:
1954 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 337 322
Size: 15 3770
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 799 1577 1767 2559 799
Size: 778 190 792 2025
Fragments arranged by size:
2025 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 173 738 764 799 1410 1503 1577 1767 1829
Size: 565 26 35 611 93 74 190 62
Cuts at: 1829 2559 173
Size: 730 1399
Fragments arranged by size:
1399 730 611 565 190 93 74 62
35 26
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 689 1783 1964 2173 689
Size: 1094 181 209 2301
Fragments arranged by size:
2301 1094 209 181
BssSI C'ACGA_G
Cuts at: 235 2056 2414 235
Size: 1821 358 1606
Fragments arranged by size:
1821 1606 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstEII G'GTnAC_C
Cuts at: 709 709
Size: 3785
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3785
BstYI r'GATC_y
Cuts at: 1 314 429 810 1277 1300 1635 1881 2893
Size: 313 115 381 467 23 335 246 1012
Cuts at: 2893 2979 2991 3337 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 381 346 335 313 246
115 86 23 12
BtgI C'CryG_G
Cuts at: 107 299 107
Size: 192 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 425 603 1735 1916 2079 425
Size: 178 1132 181 163 2131
Fragments arranged by size:
2131 1132 181 178 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 302 1370 1544 1935 1962 3307 302
Size: 1068 174 391 27 1345 780
Fragments arranged by size:
1345 1068 780 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 365 578 1808 2018 365
Size: 213 1230 210 2132
Fragments arranged by size:
2132 1230 213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 389 408 1610 2042 2789 3057 172
Size: 217 19 1202 432 747 268 900
Fragments arranged by size:
1202 900 747 432 268 217 19
EcoNI CCTnn'n_nnAGG
Cuts at: 265 265
Size: 3785
EcoO109I rG'GnC_Cy
Cuts at: 222 549 735 924 222
Size: 327 186 189 3083
Fragments arranged by size:
3083 327 189 186
EcoRI G'AATT_C
Cuts at: 454 789 454
Size: 335 3450
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 1219 1467 2489 3468 1219
Size: 248 1022 979 1536
Fragments arranged by size:
1536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 751 783 1627 1657 1659 1689 479
Size: 32 240 32 844 30 2 30 2575
Fragments arranged by size:
2575 844 240 32 32 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 648 2456 2640 648
Size: 1808 184 1793
Fragments arranged by size:
1808 1793 184
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 112 166 1774 1901 112
Size: 54 1608 127 1996
Fragments arranged by size:
1996 1608 127 54
MspA1I CmG'CkG
Cuts at: 183 508 742 896 1210 1570 2583 2828 183
Size: 325 234 154 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 360 325 314 245 234 154
NaeI GCC'GGC
Cuts at: 691 1966 691
Size: 1275 2510
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NcoI C'CATG_G
Cuts at: 107 107
Size: 3785
NgoMIV G'CCGG_C
Cuts at: 689 1964 689
Size: 1275 2510
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PflMI CCAn_nnn'nTGG
Cuts at: 300 300
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PmlI CAC'GTG
Cuts at: 168 168
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 222 549 222
Size: 327 3458
PspOMI G'GGCC_C
Cuts at: 734 734
Size: 3785
PstI C_TGCA'G
Cuts at: 211 832 1517 211
Size: 621 685 2479
Fragments arranged by size:
2479 685 621
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 183 508 1210 1570 183
Size: 325 702 360 2398
Fragments arranged by size:
2398 702 360 325
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 578 1808 2018 578
Size: 1230 210 2345
Fragments arranged by size:
2345 1230 210
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SexAI A'CCwGG_T
Cuts at: 467 467
Size: 3785
SfcI C'TryA_G
Cuts at: 33 207 828 1513 2506 2697 33
Size: 174 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 191 174
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 437 940 1855 2347 2609 437
Size: 503 915 492 262 1613
Fragments arranged by size:
1613 915 503 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StyI C'CwwG_G
Cuts at: 107 919 1273 107
Size: 812 354 2619
Fragments arranged by size:
2619 812 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 3300 3300
Size: 3785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 3302 3334 605
Size: 32 2665 32 1056
Fragments arranged by size:
2665 1056 32 32
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XhoI C'TCGA_G
Cuts at: 437 437
Size: 3785
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
XmnI GAAnn'nnTTC
Cuts at: 316 454 316
Size: 138 3647
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI