Restriction map for
pF1KB5420
(Circular) (Six-base) MAPSORT of: pF1KB5420.seq Check: 7366 from: 1 to: 4415
pF1KB5420 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1453 1453
Size: 4415
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 551 624 703 808 846 1153 1256 1374
Size: 368 73 79 105 38 307 103 118
Cuts at: 1374 2240 2672 3025 3687 183
Size: 866 432 353 662 911
Fragments arranged by size:
911 866 662 432 368 353 307 118
105 103 79 73 38
AfeI AGC'GCT
Cuts at: 1315 1315
Size: 4415
AflIII A'CryG_T
Cuts at: 1767 3567 3848 1767
Size: 1800 281 2334
Fragments arranged by size:
2334 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 891 891
Size: 4415
AleI CACnn'nnGTG
Cuts at: 724 724
Size: 4415
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1096 1128 1096
Size: 32 4383
AlwNI CAG_nnn'CTG
Cuts at: 1720 3158 1720
Size: 1438 2977
ApaLI G'TGCA_C
Cuts at: 1272 3253 1272
Size: 1981 2434
ApoI r'AATT_y
Cuts at: 1419 3768 4024 1419
Size: 2349 256 1810
Fragments arranged by size:
2349 1810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 1435 1435
Size: 4415
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 472 1294 1431 1845 2093 2128 472
Size: 822 137 414 248 35 2759
Fragments arranged by size:
2759 822 414 248 137 35
BanII G_rGCy'C
Cuts at: 254 1429 2459 254
Size: 1175 1030 2210
Fragments arranged by size:
2210 1175 1030
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbsI GAAGACnn'nnnn_
Cuts at: 796 796
Size: 4415
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3369 4415 2313
Size: 1056 1046 2313
Fragments arranged by size:
2313 1056 1046
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglII A'GATC_T
Cuts at: 137 1930 3967 137
Size: 1793 2037 585
Fragments arranged by size:
2037 1793 585
BlpI GC'TnA_GC
Cuts at: 1527 1527
Size: 4415
Bme1580I G_kGCm'C
Cuts at: 215 1276 1299 2040 2133 3257 215
Size: 1061 23 741 93 1124 1373
Fragments arranged by size:
1373 1124 1061 741 93 23
BmrI ACTGGGnnnn_n'
Cuts at: 209 616 964 1017 1286 1627 1708 2043 4187
Size: 407 348 53 269 341 81 335 2144
Cuts at: 4187 209
Size: 437
Fragments arranged by size:
2144 437 407 348 341 335 269 81
53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 799 781
Size: 18 4397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1591 2470 3184 3482 103
Size: 1488 879 714 298 1036
Fragments arranged by size:
1488 1036 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 199 4119 199
Size: 3920 495
BsaAI yAC'GTr
Cuts at: 1768 1768
Size: 4415
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 1205 1846 2094 2796 4292 1205
Size: 641 248 702 1496 1328
Fragments arranged by size:
1496 1328 702 641 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3214 3361 3635 1604
Size: 521 678 411 147 274 2384
Fragments arranged by size:
2384 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 653 683 653
Size: 30 4385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1327 1366 1327
Size: 39 4376
BseYI C'CCAG_C
Cuts at: 907 1356 1840 2598 3263 3671 907
Size: 449 484 758 665 408 1651
Fragments arranged by size:
1651 758 665 484 449 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1058 650
Size: 408 4007
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3233 104
Size: 1853 46 1230 1286
Fragments arranged by size:
1853 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 254 311 1276 1429 2207 2397 3257 254
Size: 57 965 153 778 190 860 1412
Fragments arranged by size:
1412 965 860 778 190 153 57
Bsp1286I G_dGCh'C
Cuts at: 215 254 311 1276 1299 1429 2040 2133 2207
Size: 39 57 965 23 130 611 93 74
Cuts at: 2207 2397 2459 3257 215
Size: 190 62 798 1373
Fragments arranged by size:
1373 965 798 611 190 130 93 74
62 57 39 23
BspHI T'CATG_A
Cuts at: 611 2847 611
Size: 2236 2179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 902 1465 1679 1981 2362 902
Size: 563 214 302 381 2955
Fragments arranged by size:
2955 563 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 1378 2413 2594 1378
Size: 1035 181 3199
Fragments arranged by size:
3199 1035 181
BsrGI T'GTAC_A
Cuts at: 944 944
Size: 4415
BssSI C'ACGA_G
Cuts at: 2686 3394 2686
Size: 708 3707
BstAPI GCAn_nnn'nTGC
Cuts at: 1792 1792
Size: 4415
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 726 759 1195 726
Size: 33 436 3946
Fragments arranged by size:
3946 436 33
BstYI r'GATC_y
Cuts at: 1 122 137 987 1440 1907 1930 2265 2511
Size: 121 15 850 453 467 23 335 246
Cuts at: 2511 2817 2829 2915 3967 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 850 467 453 449 335 306 246
121 86 23 15 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 492 1233 2365 2546 2709 492
Size: 741 1132 181 163 2198
Fragments arranged by size:
2198 1132 741 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 1658
Size: 4415
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 2121 3465 2121
Size: 1344 3071
EaeI y'GGCC_r
Cuts at: 596 1319 1403 2000 2174 2565 2592 3937 596
Size: 723 84 597 174 391 27 1345 1074
Fragments arranged by size:
1345 1074 723 597 391 174 84 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 184 202 1172 1343 2438 2648 184
Size: 18 970 171 1095 210 1951
Fragments arranged by size:
1951 1095 970 210 171 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 3365 3511 4225 2623
Size: 742 146 714 2813
Fragments arranged by size:
2813 742 714 146
EcoICRI GAG'CTC
Cuts at: 252 1427 252
Size: 1175 3240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 551 624 703 781 799 808 846 1153
Size: 368 73 79 78 18 9 38 307
Cuts at: 1153 1256 1374 2240 2672 3025 3687 183
Size: 103 118 866 432 353 662 911
Fragments arranged by size:
911 866 662 432 368 353 307 118
103 79 78 73 38 18 9
EcoO109I rG'GnC_Cy
Cuts at: 243 489 1554 243
Size: 246 1065 3104
Fragments arranged by size:
3104 1065 246
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 655 2196 655
Size: 1541 2874
FspAI rTGC'GCAy
Cuts at: 655 655
Size: 4415
HaeII r_GCGC'y
Cuts at: 1317 1849 2097 3327 4098 1317
Size: 532 248 1230 771 1634
Fragments arranged by size:
1634 1230 771 532 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 877 909 1149 1181 2257 2287 2289 2319 877
Size: 32 240 32 1076 30 2 30 2973
Fragments arranged by size:
2973 1076 240 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1140 1454 1140
Size: 314 4101
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3174 3358 3174
Size: 184 4231
MscI TGG'CCA
Cuts at: 598 1321 1405 2176 598
Size: 723 84 771 2837
Fragments arranged by size:
2837 771 723 84
MslI CAynn'nnrTG
Cuts at: 724 835 2404 2531 724
Size: 111 1569 127 2608
Fragments arranged by size:
2608 1569 127 111
MspA1I CmG'CkG
Cuts at: 933 1526 1840 2200 2984 3229 933
Size: 593 314 360 784 245 2119
Fragments arranged by size:
2119 784 593 360 314 245
NaeI GCC'GGC
Cuts at: 1380 2596 1380
Size: 1216 3199
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NgoMIV G'CCGG_C
Cuts at: 1378 2594 1378
Size: 1216 3199
NspI r_CATG'y
Cuts at: 1468 1968 3571 1468
Size: 500 1603 2312
Fragments arranged by size:
2312 1603 500
PciI A'CATG_T
Cuts at: 3567 3567
Size: 4415
PflMI CCAn_nnn'nTGG
Cuts at: 1053 1199 1053
Size: 146 4269
PfoI T'CCnGG_A
Cuts at: 254 758 254
Size: 504 3911
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 489 489
Size: 4415
PstI C_TGCA'G
Cuts at: 1462 2147 1462
Size: 685 3730
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 933 1840 2200 933
Size: 907 360 3148
Fragments arranged by size:
3148 907 360
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 254 1429 254
Size: 1175 3240
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SanDI GG'GwC_CC
Cuts at: 489 489
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SexAI A'CCwGG_T
Cuts at: 1256 1256
Size: 4415
SfcI C'TryA_G
Cuts at: 33 1458 2143 3111 3302 33
Size: 1425 685 968 191 1146
Fragments arranged by size:
1425 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 118 1570 2485 3199 3461 118
Size: 1452 915 714 262 1072
Fragments arranged by size:
1452 1072 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
StuI AGG'CCT
Cuts at: 776 776
Size: 4415
StyI C'CwwG_G
Cuts at: 1094 1400 1406 1549 1903 1094
Size: 306 6 143 354 3606
Fragments arranged by size:
3606 354 306 143 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1174 1998 2131 2590 2799 1174
Size: 824 133 459 209 2790
Fragments arranged by size:
2790 824 459 209 133
TatI w'GTAC_w
Cuts at: 944 1325 3930 944
Size: 381 2605 1429
Fragments arranged by size:
2605 1429 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4383
Tth111I GACn'n_nGTC
Cuts at: 1161 2212 1161
Size: 1051 3364
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1910 1910
Size: 4415
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI