Restriction map for
pF1KB5413
(Circular) (Six-base) MAPSORT of: pF1KB5413.seq Check: 2290 from: 1 to: 3752
pF1KB5413 3752 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1577 2009 2756 3024 682
Size: 895 432 747 268 1410
Fragments arranged by size:
1410 895 747 432 268
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 422 1057 2624 422
Size: 635 1567 1550
Fragments arranged by size:
1567 1550 635
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 516 772 516
Size: 256 3496
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 768 1182 1430 1465 768
Size: 414 248 35 3055
Fragments arranged by size:
3055 414 248 35
BanII G_rGCy'C
Cuts at: 557 766 1796 557
Size: 209 1030 2513
Fragments arranged by size:
2513 1030 209
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BbsI GAAGACnn'nnnn_
Cuts at: 159 159
Size: 3752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3718
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 689 1272 689
Size: 583 3169
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 232 864 232
Size: 632 3120
Bme1580I G_kGCm'C
Cuts at: 140 1377 1470 2526 140
Size: 1237 93 1056 1366
Fragments arranged by size:
1366 1237 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 542 2119 542
Size: 1577 2175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 392
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1807 2299 2597 928
Size: 879 492 298 2083
Fragments arranged by size:
2083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 492 623 941 1462 2140 2414 2561 2972 492
Size: 131 318 521 678 274 147 411 1272
Fragments arranged by size:
1272 678 521 411 318 274 147 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 313 343 313
Size: 30 3722
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 559 207
Size: 352 3400
BseYI C'CCAG_C
Cuts at: 544 1177 1935 2512 3008 544
Size: 633 758 577 496 1288
Fragments arranged by size:
1288 758 633 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 698 1294 1340 2548 104
Size: 594 596 46 1208 1308
Fragments arranged by size:
1308 1208 596 594 46
BsiHKAI G_wGCw'C
Cuts at: 557 766 1544 1734 2526 557
Size: 209 778 190 792 1783
Fragments arranged by size:
1783 792 778 209 190
Bsp1286I G_dGCh'C
Cuts at: 140 557 766 1377 1470 1544 1734 1796 2526
Size: 417 209 611 93 74 190 62 730
Cuts at: 2526 140
Size: 1366
Fragments arranged by size:
1366 730 611 417 209 190 93 74
62
BspEI T'CCGG_A
Cuts at: 623 2972 623
Size: 2349 1403
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 802 1016 1318 1699 236
Size: 566 214 302 381 2289
Fragments arranged by size:
2289 566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 503 777 1244 1267 1602 1848 2860 2946
Size: 502 274 467 23 335 246 1012 86
Cuts at: 2946 2958 3304 1
Size: 12 346 449
Fragments arranged by size:
1012 502 467 449 346 335 274 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 636 1702 1883 2046 636
Size: 1066 181 163 2342
Fragments arranged by size:
2342 1066 181 163
BtsI GCAGTG_nn'
Cuts at: 462 641 995 462
Size: 179 354 3219
Fragments arranged by size:
3219 354 179
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DraIII CAC_nnn'GTG
Cuts at: 338 539 338
Size: 201 3551
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 413 695 1337 1511 1902 1929 3274 413
Size: 282 642 174 391 27 1345 891
Fragments arranged by size:
1345 891 642 391 282 174 27
EagI C'GGCC_G
Cuts at: 695 1337 695
Size: 642 3110
EarI CTCTTCn'nnn_
Cuts at: 562 1775 1985 562
Size: 1213 210 2329
Fragments arranged by size:
2329 1213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 555 764 555
Size: 209 3543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 682 1577 2009 2756 3024 392
Size: 290 895 432 747 268 1120
Fragments arranged by size:
1120 895 747 432 290 268
EcoO109I rG'GnC_Cy
Cuts at: 595 891 595
Size: 296 3456
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 1594 1624 1626 1656 313
Size: 32 1249 30 2 30 2409
Fragments arranged by size:
2409 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 351 791 351
Size: 440 3312
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 415 1513 415
Size: 1098 2654
MslI CAynn'nnrTG
Cuts at: 1741 1868 1741
Size: 127 3625
MspA1I CmG'CkG
Cuts at: 236 326 863 1177 1537 2550 2795 236
Size: 90 537 314 360 1013 245 1193
Fragments arranged by size:
1193 1013 537 360 314 245 90
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 315 805 1305 2212 315
Size: 490 500 907 1855
Fragments arranged by size:
1855 907 500 490
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PflMI CCAn_nnn'nTGG
Cuts at: 422 422
Size: 3752
PfoI T'CCnGG_A
Cuts at: 484 484
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PpuMI rG'GwC_Cy
Cuts at: 595 595
Size: 3752
PspXI vC'TCGA_Gb
Cuts at: 516 516
Size: 3752
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 321 1947 321
Size: 1626 2126
SacI G_AGCT'C
Cuts at: 557 766 557
Size: 209 3543
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 562 1775 1985 562
Size: 1213 210 2329
Fragments arranged by size:
2329 1213 210
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 516 907 1822 2314 2576 516
Size: 391 915 492 262 1692
Fragments arranged by size:
1692 915 492 391 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StuI AGG'CCT
Cuts at: 522 522
Size: 3752
StyI C'CwwG_G
Cuts at: 886 1240 886
Size: 354 3398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 1247 342
Size: 905 2847
XhoI C'TCGA_G
Cuts at: 516 516
Size: 3752
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI