Restriction map for
pF1KB5412
(Circular) (Six-base) MAPSORT of: pF1KB5412.seq Check: 7317 from: 1 to: 4169
pF1KB5412 4169 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1994 2426 3173 3441 1994
Size: 432 747 268 2722
Fragments arranged by size:
2722 747 432 268
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 682
Size: 32 4137
AlwNI CAG_nnn'CTG
Cuts at: 337 1105 1474 3041 337
Size: 768 369 1567 1465
Fragments arranged by size:
1567 1465 768 369
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 436 524 552 902 1173 3522 3778 436
Size: 88 28 350 271 2349 256 827
Fragments arranged by size:
2349 827 350 271 256 88 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 187 1189 187
Size: 1002 3167
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 1185 1599 1847 1882 1185
Size: 414 248 35 3472
Fragments arranged by size:
3472 414 248 35
BanII G_rGCy'C
Cuts at: 257 1183 2213 257
Size: 926 1030 2213
Fragments arranged by size:
2213 1030 926
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 600 1689 600
Size: 1089 3080
BglII A'GATC_T
Cuts at: 972 1684 3721 972
Size: 712 2037 1420
Fragments arranged by size:
2037 1420 712
BlpI GC'TnA_GC
Cuts at: 649 1281 649
Size: 632 3537
Bme1580I G_kGCm'C
Cuts at: 482 1794 1887 2943 482
Size: 1312 93 1056 1708
Fragments arranged by size:
1708 1312 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1134 1381 1462 1797 3941 1134
Size: 247 81 335 2144 1362
Fragments arranged by size:
2144 1362 335 247 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 723 616
Size: 107 4062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 869 1345 2224 2716 3014 141
Size: 728 476 879 492 298 1296
Fragments arranged by size:
1296 879 728 492 476 298
BsaI GGTCTCn'nnnn_
Cuts at: 239 3873 239
Size: 3634 535
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 1600 1848 2550 4046 1600
Size: 248 702 1496 1723
Fragments arranged by size:
1723 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 709 739 709
Size: 30 4139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 241 247 805 1033 238
Size: 3 6 558 228 3374
Fragments arranged by size:
3374 558 228 6 3
BseYI C'CCAG_C
Cuts at: 746 1594 2352 2929 3425 746
Size: 848 758 577 496 1490
Fragments arranged by size:
1490 848 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 1183 1961 2151 2943 257
Size: 926 778 190 792 1483
Fragments arranged by size:
1483 926 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 196 729 196
Size: 533 3636
Bsp1286I G_dGCh'C
Cuts at: 257 482 1183 1794 1887 1961 2151 2213 2943
Size: 225 701 611 93 74 190 62 730
Cuts at: 2943 257
Size: 1483
Fragments arranged by size:
1483 730 701 611 225 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 807 2081 807
Size: 1274 2895
BsrFI r'CCGG_y
Cuts at: 147 2167 2348 2557 147
Size: 2020 181 209 1759
Fragments arranged by size:
2020 1759 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 307 819 999 1546 307
Size: 512 180 547 2930
Fragments arranged by size:
2930 547 512 180
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 972 1194 1661 1684 2019 2265 3277 3363
Size: 971 222 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 971 467 449 346 335 246 222
86 23 12
BtgI C'CryG_G
Cuts at: 484 614 484
Size: 130 4039
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 1754 1928 2319 2346 3691 1754
Size: 174 391 27 1345 2232
Fragments arranged by size:
2232 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 440 790 1031 2192 2402 440
Size: 350 241 1161 210 2207
Fragments arranged by size:
2207 1161 350 241 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 255 1181 255
Size: 926 3243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 723 1994 2426 3173 3441 616
Size: 107 1271 432 747 268 1344
Fragments arranged by size:
1344 1271 747 432 268 107
EcoNI CCTnn'n_nnAGG
Cuts at: 262 964 262
Size: 702 3467
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 1603 1851 2873 3852 1603
Size: 248 1022 979 1920
Fragments arranged by size:
1920 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 653 685 2011 2041 2043 2073 181
Size: 32 440 32 1326 30 2 30 2277
Fragments arranged by size:
2277 1326 440 32 32 30 30 2
HincII GTy'rAC
Cuts at: 492 1208 492
Size: 716 3453
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 1930 1930
Size: 4169
MslI CAynn'nnrTG
Cuts at: 2158 2285 2158
Size: 127 4042
MspA1I CmG'CkG
Cuts at: 475 541 825 1280 1594 1954 2967 3212 475
Size: 66 284 455 314 360 1013 245 1432
Fragments arranged by size:
1432 1013 455 360 314 284 245 66
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 484 484
Size: 4169
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 1222 1722 2629 1222
Size: 500 907 2762
Fragments arranged by size:
2762 907 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PfoI T'CCnGG_A
Cuts at: 632 632
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 700 732 700
Size: 32 4137
PstI C_TGCA'G
Cuts at: 236 1050 1070 1216 1901 236
Size: 814 20 146 685 2504
Fragments arranged by size:
2504 814 685 146 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 475 541 1594 1954 475
Size: 66 1053 360 2690
Fragments arranged by size:
2690 1053 360 66
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 257 1183 257
Size: 926 3243
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 440 790 2192 2402 440
Size: 350 1402 210 2207
Fragments arranged by size:
2207 1402 350 210
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 232 1046 1066 1212 1897 2890 3081 33
Size: 199 814 20 146 685 993 191 1121
Fragments arranged by size:
1121 993 814 685 199 191 146 20
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 120 884 1324 2239 2731 2993 120
Size: 764 440 915 492 262 1296
Fragments arranged by size:
1296 915 764 492 440 262
SpeI A'CTAG_T
Cuts at: 67 996 67
Size: 929 3240
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StyI C'CwwG_G
Cuts at: 484 1303 1657 484
Size: 819 354 2996
Fragments arranged by size:
2996 819 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1752 1885 2344 2553 1752
Size: 133 459 209 3368
Fragments arranged by size:
3368 459 209 133
TatI w'GTAC_w
Cuts at: 3684 3684
Size: 4169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
XmnI GAAnn'nnTTC
Cuts at: 512 512
Size: 4169
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI