Restriction map for
pF1KB5411
(Circular) (Six-base) MAPSORT of: pF1KB5411.seq Check: 2299 from: 1 to: 4898
pF1KB5411 4898 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1936 1936
Size: 4898
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 443 751 1441 1489 2723 3155 3902 4170
Size: 320 308 690 48 1234 432 747 268
Cuts at: 4170 123
Size: 851
Fragments arranged by size:
1234 851 747 690 432 320 308 268
48
AflIII A'CryG_T
Cuts at: 2250 3354 4331 2250
Size: 1104 977 2817
Fragments arranged by size:
2817 1104 977
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AhdI GACnn_n'nnGTC
Cuts at: 1620 1620
Size: 4898
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 1726 817
Size: 909 3989
AlwNI CAG_nnn'CTG
Cuts at: 747 1321 1479 2203 3770 747
Size: 574 158 724 1567 1875
Fragments arranged by size:
1875 1567 724 574 158
ApaLI G'TGCA_C
Cuts at: 3668 3668
Size: 4898
ApoI r'AATT_y
Cuts at: 403 1902 4251 4507 403
Size: 1499 2349 256 794
Fragments arranged by size:
2349 1499 794 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4898
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 1127 1868 1918 1127
Size: 741 50 4107
Fragments arranged by size:
4107 741 50
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 1914 2328 2576 2611 1914
Size: 414 248 35 4201
Fragments arranged by size:
4201 414 248 35
BanII G_rGCy'C
Cuts at: 275 1892 1912 2942 275
Size: 1617 20 1030 2231
Fragments arranged by size:
2231 1617 1030 20
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4650
BbsI GAAGACnn'nnnn_
Cuts at: 945 945
Size: 4898
BbvCI CC'TCA_GC
Cuts at: 1430 1430
Size: 4898
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3557 4898 2796
Size: 761 1341 2796
Fragments arranged by size:
2796 1341 761
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4898
BglI GCCn_nnn'nGGC
Cuts at: 547 1309 1318 1456 547
Size: 762 9 138 3989
Fragments arranged by size:
3989 762 138 9
BglII A'GATC_T
Cuts at: 497 866 2413 4450 497
Size: 369 1547 2037 945
Fragments arranged by size:
2037 1547 945 369
BlpI GC'TnA_GC
Cuts at: 1322 2010 1322
Size: 688 4210
Bme1580I G_kGCm'C
Cuts at: 796 2523 2616 3672 796
Size: 1727 93 1056 2022
Fragments arranged by size:
2022 1727 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 566 2110 2191 2526 4670 566
Size: 1544 81 335 2144 794
Fragments arranged by size:
2144 1544 794 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 982 1546 769
Size: 213 564 4121
Fragments arranged by size:
4121 564 213
Bpu10I CC'TnA_GC
Cuts at: 740 1430 740
Size: 690 4208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1717 2074 2953 3445 3743 133
Size: 1584 357 879 492 298 1288
Fragments arranged by size:
1584 1288 879 492 357 298
BsaI GGTCTCn'nnnn_
Cuts at: 4602 4602
Size: 4898
BsaAI yAC'GTr
Cuts at: 1547 2251 1547
Size: 704 4194
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 197 2329 2577 3279 4775 197
Size: 2132 248 702 1496 320
Fragments arranged by size:
2132 1496 702 320 248
BsaWI w'CCGG_w
Cuts at: 648 779 2087 2608 3286 3560 3707 4118 648
Size: 131 1308 521 678 274 147 411 1428
Fragments arranged by size:
1428 1308 678 521 411 274 147 131
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 676 957 1047 1102 1162 1194 1255 1297
Size: 234 281 90 55 60 32 61 42
Cuts at: 1297 1300 1381 1384 1447 1458 442
Size: 3 81 3 63 11 3882
Fragments arranged by size:
3882 281 234 90 81 63 61 60
55 42 32 11 3 3
BseYI C'CCAG_C
Cuts at: 2323 3081 3658 4154 2323
Size: 758 577 496 3067
Fragments arranged by size:
3067 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 1774
Size: 4898
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 527 692 1892 1912 2690 2880 3672 527
Size: 165 1200 20 778 190 792 1753
Fragments arranged by size:
1753 1200 792 778 190 165 20
BsmI GAATG_Cn'
Cuts at: 423 423
Size: 4898
Bsp1286I G_dGCh'C
Cuts at: 275 527 692 796 1892 1912 2523 2616 2690
Size: 252 165 104 1096 20 611 93 74
Cuts at: 2690 2880 2942 3672 275
Size: 190 62 730 1501
Fragments arranged by size:
1501 1096 730 611 252 190 165 104
93 74 62 20
BspEI T'CCGG_A
Cuts at: 648 779 4118 648
Size: 131 3339 1428
Fragments arranged by size:
3339 1428 131
BspHI T'CATG_A
Cuts at: 357 4074 357
Size: 3717 1181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1487 1948 2162 2464 2845 1487
Size: 461 214 302 381 3540
Fragments arranged by size:
3540 461 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1113 3190 3244 4728 4893 50
Size: 1063 2077 54 1484 165 55
Fragments arranged by size:
2077 1484 1063 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2810 2810
Size: 4898
BsrFI r'CCGG_y
Cuts at: 2896 3077 3286 2896
Size: 181 209 4508
Fragments arranged by size:
4508 209 181
BssSI C'ACGA_G
Cuts at: 1161 3169 3527 1161
Size: 2008 358 2532
Fragments arranged by size:
2532 2008 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1332 1395 1479 2275 1332
Size: 63 84 796 3955
Fragments arranged by size:
3955 796 84 63
BstBI TT'CG_AA
Cuts at: 3259 3259
Size: 4898
BstYI r'GATC_y
Cuts at: 1 497 866 1923 2390 2413 2748 2994 4006
Size: 496 369 1057 467 23 335 246 1012
Cuts at: 4006 4092 4104 4450 1
Size: 86 12 346 449
Fragments arranged by size:
1057 1012 496 467 449 369 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 512 512
Size: 4898
BtgI C'CryG_G
Cuts at: 107 191 877 1661 107
Size: 84 686 784 3344
Fragments arranged by size:
3344 784 686 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 105 2848 3029 3192 105
Size: 2743 181 163 1811
Fragments arranged by size:
2743 1811 181 163
BtsI GCAGTG_nn'
Cuts at: 2141 2141
Size: 4898
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 2604 3462 2604
Size: 858 4040
EaeI y'GGCC_r
Cuts at: 110 398 1214 2483 2657 3048 3075 4420 110
Size: 288 816 1269 174 391 27 1345 588
Fragments arranged by size:
1345 1269 816 588 391 288 174 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 419 1500 1880 2921 3131 419
Size: 1081 380 1041 210 2186
Fragments arranged by size:
2186 1081 1041 380 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1513 3106 3416 3562 4708 1513
Size: 1593 310 146 1146 1703
Fragments arranged by size:
1703 1593 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1890 1910 1890
Size: 20 4878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 443 751 769 982 1441 1489 1546 2723
Size: 320 308 18 213 459 48 57 1177
Cuts at: 2723 3155 3902 4170 123
Size: 432 747 268 851
Fragments arranged by size:
1177 851 747 459 432 320 308 268
213 57 48 18
EcoO109I rG'GnC_Cy
Cuts at: 240 744 1476 2037 240
Size: 504 732 561 3101
Fragments arranged by size:
3101 732 561 504
EcoRI G'AATT_C
Cuts at: 1902 1902
Size: 4898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 261 888 1866 2917 261
Size: 627 978 1051 2242
Fragments arranged by size:
2242 1051 978 627
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 1122 1380 1862 2332 2580 3602 4581 1122
Size: 258 482 470 248 1022 979 1439
Fragments arranged by size:
1439 1022 979 482 470 258 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1638 1692 1724 2740 2770 2772 2802 1606
Size: 32 54 32 1016 30 2 30 3702
Fragments arranged by size:
3702 1016 54 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1937 1937
Size: 4898
HindIII A'AGCT_T
Cuts at: 973 1596 973
Size: 623 4275
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4650
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MfeI C'AATT_G
Cuts at: 213 213
Size: 4898
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1829 3569 3753 1154
Size: 675 1740 184 2299
Fragments arranged by size:
2299 1740 675 184
MscI TGG'CCA
Cuts at: 400 1216 2659 400
Size: 816 1443 2639
Fragments arranged by size:
2639 1443 816
MslI CAynn'nnrTG
Cuts at: 836 2887 3014 836
Size: 2051 127 2720
Fragments arranged by size:
2720 2051 127
MspA1I CmG'CkG
Cuts at: 1249 1467 2009 2323 2683 3696 3941 1249
Size: 218 542 314 360 1013 245 2206
Fragments arranged by size:
2206 1013 542 360 314 245 218
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4898
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4650
NcoI C'CATG_G
Cuts at: 107 191 1661 107
Size: 84 1470 3344
Fragments arranged by size:
3344 1470 84
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4898
NspI r_CATG'y
Cuts at: 1951 2451 3358 1951
Size: 500 907 3491
Fragments arranged by size:
3491 907 500
PasI CC'CwG_GG
Cuts at: 822 822
Size: 4898
PciI A'CATG_T
Cuts at: 3354 3354
Size: 4898
PfoI T'CCnGG_A
Cuts at: 1523 1523
Size: 4898
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 193 225 4037 4069 193
Size: 32 3812 32 1022
Fragments arranged by size:
3812 1022 32 32
PpuMI rG'GwC_Cy
Cuts at: 240 744 1476 240
Size: 504 732 3662
Fragments arranged by size:
3662 732 504
PstI C_TGCA'G
Cuts at: 302 1193 1484 1715 1945 2630 302
Size: 891 291 231 230 685 2570
Fragments arranged by size:
2570 891 685 291 231 230
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 1249 1467 2323 2683 1249
Size: 218 856 360 3464
Fragments arranged by size:
3464 856 360 218
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1892 1912 1892
Size: 20 4878
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 1500 1880 2921 3131 1500
Size: 380 1041 210 3267
Fragments arranged by size:
3267 1041 380 210
SbfI CC_TGCA'GG
Cuts at: 1484 1945 1484
Size: 461 4437
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SexAI A'CCwGG_T
Cuts at: 283 283
Size: 4898
SfcI C'TryA_G
Cuts at: 33 298 1189 1480 1711 1941 2626 3619 3810
Size: 265 891 291 231 230 685 993 191
Cuts at: 3810 33
Size: 1121
Fragments arranged by size:
1121 993 891 685 291 265 231 230
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1309 1309
Size: 4898
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4650
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 148 1127 1732 2053 2968 3460 3722 148
Size: 979 605 321 915 492 262 1324
Fragments arranged by size:
1324 979 915 605 492 321 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
StuI AGG'CCT
Cuts at: 1750 1750
Size: 4898
StyI C'CwwG_G
Cuts at: 107 191 1661 2032 2386 107
Size: 84 1470 371 354 2619
Fragments arranged by size:
2619 1470 371 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2481 2614 3073 3282 2481
Size: 133 459 209 4097
Fragments arranged by size:
4097 459 209 133
TatI w'GTAC_w
Cuts at: 302 4413 302
Size: 4111 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4415 4447 4415
Size: 32 4866
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 553 1869 2393 553
Size: 1316 524 3058
Fragments arranged by size:
3058 1316 524
XhoI C'TCGA_G
Cuts at: 1127 1127
Size: 4898
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
XmnI GAAnn'nnTTC
Cuts at: 611 611
Size: 4898
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI
SspI SwaI ZraI