Restriction map for
pF1KB5408
(Circular) (Six-base) MAPSORT of: pF1KB5408.seq Check: 1976 from: 1 to: 4364
pF1KB5408 4364 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 170 1402 170
Size: 1232 3132
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 2189 2621 3368 3636 148
Size: 2041 432 747 268 876
Fragments arranged by size:
2041 876 747 432 268
AflII C'TTAA_G
Cuts at: 757 757
Size: 4364
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 384 1069 1669 3236 384
Size: 685 600 1567 1512
Fragments arranged by size:
1567 1512 685 600
ApaLI G'TGCA_C
Cuts at: 241 3134 241
Size: 2893 1471
ApoI r'AATT_y
Cuts at: 534 911 1368 3717 3973 534
Size: 377 457 2349 256 925
Fragments arranged by size:
2349 925 457 377 256
AseI AT'TA_AT
Cuts at: 20 266 20
Size: 246 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
AvrII C'CTAG_G
Cuts at: 1050 1050
Size: 4364
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 116 123 211 654 1042 1380 1794 2042 2077
Size: 7 88 443 388 338 414 248 35
Cuts at: 2077 116
Size: 2403
Fragments arranged by size:
2403 443 414 388 338 248 88 35
7
BanII G_rGCy'C
Cuts at: 144 1190 1378 2408 144
Size: 1046 188 1030 2100
Fragments arranged by size:
2100 1046 1030 188
BbeI G_GCGC'C
Cuts at: 120 127 215 1798 2046 120
Size: 7 88 1583 248 2438
Fragments arranged by size:
2438 1583 248 88 7
BbsI GAAGACnn'nnnn_
Cuts at: 713 713
Size: 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 433 467 433
Size: 34 4330
BciVI GTATCCnnnnn_n'
Cuts at: 2262 3023 4364 2262
Size: 761 1341 2262
Fragments arranged by size:
2262 1341 761
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglI GCCn_nnn'nGGC
Cuts at: 1263 1263
Size: 4364
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 245 659 1063 1989 2082 3138 245
Size: 414 404 926 93 1056 1471
Fragments arranged by size:
1471 1056 926 414 404 93
BmrI ACTGGGnnnn_n'
Cuts at: 191 1576 1657 1992 4136 191
Size: 1385 81 335 2144 419
Fragments arranged by size:
2144 1385 419 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 685 697 1087 1318 641
Size: 44 12 390 231 3687
Fragments arranged by size:
3687 390 231 44 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 1540 2419 2911 3209 297
Size: 1243 879 492 298 1452
Fragments arranged by size:
1452 1243 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4068 4068
Size: 4364
BsaAI yAC'GTr
Cuts at: 1119 1717 1119
Size: 598 3766
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 117 124 212 1795 2043 2745 4241 117
Size: 7 88 1583 248 702 1496 240
Fragments arranged by size:
1583 1496 702 248 240 88 7
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 547
Size: 30 4334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 221 221
Size: 4364
BseYI C'CCAG_C
Cuts at: 175 474 503 658 1789 2547 3124 3620 175
Size: 299 29 155 1131 758 577 496 919
Fragments arranged by size:
1131 919 758 577 496 299 155 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 568 784 277
Size: 291 216 3857
Fragments arranged by size:
3857 291 216
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 1190 1378 2156 2346 3138 245
Size: 945 188 778 190 792 1471
Fragments arranged by size:
1471 945 792 778 190 188
BsmBI CGTCTCn'nnnn_
Cuts at: 1020 1020
Size: 4364
Bsp1286I G_dGCh'C
Cuts at: 144 245 659 1063 1190 1378 1989 2082 2156
Size: 101 414 404 127 188 611 93 74
Cuts at: 2156 2346 2408 3138 144
Size: 190 62 730 1370
Fragments arranged by size:
1370 730 611 414 404 190 188 127
101 93 74 62
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 848 858 1414 1628 1930 2311 848
Size: 10 556 214 302 381 2901
Fragments arranged by size:
2901 556 381 302 214 10
BsrBI CCG'CTC
Cuts at: 50 496 2656 2710 4194 4359 50
Size: 446 2160 54 1484 165 55
Fragments arranged by size:
2160 1484 446 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 113 430 2362 2543 2752 113
Size: 317 1932 181 209 1725
Fragments arranged by size:
1932 1725 317 209 181
BssSI C'ACGA_G
Cuts at: 508 2635 2993 508
Size: 2127 358 1879
Fragments arranged by size:
2127 1879 358
BstAPI GCAn_nnn'nTGC
Cuts at: 249 546 1254 1741 249
Size: 297 708 487 2872
Fragments arranged by size:
2872 708 487 297
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstEII G'GTnAC_C
Cuts at: 738 738
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 330 886 330
Size: 556 3808
BstYI r'GATC_y
Cuts at: 1 1389 1856 1879 2214 2460 3472 3558 3570
Size: 1388 467 23 335 246 1012 86 12
Cuts at: 3570 3916 1
Size: 346 449
Fragments arranged by size:
1388 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 743 1131 107
Size: 636 388 3340
Fragments arranged by size:
3340 636 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 177 2314 2495 2658 177
Size: 2137 181 163 1883
Fragments arranged by size:
2137 1883 181 163
BtsI GCAGTG_nn'
Cuts at: 571 1607 571
Size: 1036 3328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 981 1016 981
Size: 35 4329
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 1002 1949 2123 2514 2541 3886 1002
Size: 947 174 391 27 1345 1480
Fragments arranged by size:
1480 1345 947 391 174 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 904 2387 2597 904
Size: 1483 210 2671
Fragments arranged by size:
2671 1483 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 218 1281 2572 2882 3028 4174 218
Size: 1063 1291 310 146 1146 408
Fragments arranged by size:
1291 1146 1063 408 310 146
EcoICRI GAG'CTC
Cuts at: 1188 1376 1188
Size: 188 4176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 641 685 697 1087 1318 2189 2621 3368
Size: 493 44 12 390 231 871 432 747
Cuts at: 3368 3636 148
Size: 268 876
Fragments arranged by size:
876 871 747 493 432 390 268 231
44 12
EcoNI CCTnn'n_nnAGG
Cuts at: 667 667
Size: 4364
EcoO109I rG'GnC_Cy
Cuts at: 448 721 753 1196 1503 448
Size: 273 32 443 307 3309
Fragments arranged by size:
3309 443 307 273 32
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 120 127 215 276 927 1798 2046 3068 4047
Size: 7 88 61 651 871 248 1022 979
Cuts at: 4047 120
Size: 437
Fragments arranged by size:
1022 979 871 651 437 248 88 61
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 2206 2236 2238 2268 547
Size: 32 1627 30 2 30 2643
Fragments arranged by size:
2643 1627 32 30 30 2
HincII GTy'rAC
Cuts at: 171 1403 171
Size: 1232 3132
KasI G'GCGC_C
Cuts at: 116 123 211 1794 2042 116
Size: 7 88 1583 248 2438
Fragments arranged by size:
2438 1583 248 88 7
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 995 3035 3219 995
Size: 2040 184 2140
Fragments arranged by size:
2140 2040 184
MscI TGG'CCA
Cuts at: 1004 2125 1004
Size: 1121 3243
MslI CAynn'nnrTG
Cuts at: 299 1141 2353 2480 299
Size: 842 1212 127 2183
Fragments arranged by size:
2183 1212 842 127
MspA1I CmG'CkG
Cuts at: 786 798 1245 1475 1789 2149 3162 3407 786
Size: 12 447 230 314 360 1013 245 1743
Fragments arranged by size:
1743 1013 447 360 314 245 230 12
NaeI GCC'GGC
Cuts at: 115 2545 115
Size: 2430 1934
NarI GG'CG_CC
Cuts at: 117 124 212 1795 2043 117
Size: 7 88 1583 248 2438
Fragments arranged by size:
2438 1583 248 88 7
NcoI C'CATG_G
Cuts at: 107 1131 107
Size: 1024 3340
NgoMIV G'CCGG_C
Cuts at: 113 2543 113
Size: 2430 1934
NspI r_CATG'y
Cuts at: 1417 1917 2824 1417
Size: 500 907 2957
Fragments arranged by size:
2957 907 500
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 753 1196 753
Size: 443 3921
PstI C_TGCA'G
Cuts at: 254 360 578 859 1411 2096 254
Size: 106 218 281 552 685 2522
Fragments arranged by size:
2522 685 552 281 218 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 786 798 1245 1789 2149 786
Size: 12 447 544 360 3001
Fragments arranged by size:
3001 544 447 360 12
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1190 1378 1190
Size: 188 4176
SalI G'TCGA_C
Cuts at: 169 1401 169
Size: 1232 3132
SanDI GG'GwC_CC
Cuts at: 1196 1196
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 859 1411 859
Size: 552 3812
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SfcI C'TryA_G
Cuts at: 33 250 356 574 855 1407 2092 3085 3276
Size: 217 106 218 281 552 685 993 191
Cuts at: 3276 33
Size: 1121
Fragments arranged by size:
1121 993 685 552 281 218 217 191
106
SfoI GGC'GCC
Cuts at: 118 125 213 1796 2044 118
Size: 7 88 1583 248 2438
Fragments arranged by size:
2438 1583 248 88 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 276 757 1519 2434 2926 3188 276
Size: 481 762 915 492 262 1452
Fragments arranged by size:
1452 915 762 492 481 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StuI AGG'CCT
Cuts at: 763 853 1055 763
Size: 90 202 4072
Fragments arranged by size:
4072 202 90
StyI C'CwwG_G
Cuts at: 107 437 1050 1131 1191 1498 1852 107
Size: 330 613 81 60 307 354 2619
Fragments arranged by size:
2619 613 354 330 307 81 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1947 2080 2539 2748 1947
Size: 133 459 209 3563
Fragments arranged by size:
3563 459 209 133
TatI w'GTAC_w
Cuts at: 3879 3879
Size: 4364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 993 2161 993
Size: 1168 3196
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 1859 327
Size: 1532 2832
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI