Restriction map for
pF1KB5406
(Circular) (Six-base) MAPSORT of: pF1KB5406.seq Check: 9342 from: 1 to: 4106
pF1KB5406 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 264 264
Size: 4106
AccI GT'mk_AC
Cuts at: 193 1144 193
Size: 951 3155
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 646 1931 2363 3110 3378 110
Size: 536 1285 432 747 268 838
Fragments arranged by size:
1285 838 747 536 432 268
AflIII A'CryG_T
Cuts at: 1458 2562 3539 1458
Size: 1104 977 2025
Fragments arranged by size:
2025 1104 977
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 1071 1411 2978 1071
Size: 340 1567 2199
Fragments arranged by size:
2199 1567 340
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AseI AT'TA_AT
Cuts at: 20 541 20
Size: 521 3585
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 703 1122 1536 1784 1819 703
Size: 419 414 248 35 2990
Fragments arranged by size:
2990 419 414 248 35
BanII G_rGCy'C
Cuts at: 1120 2150 1120
Size: 1030 3076
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 422 1621 3658 422
Size: 1199 2037 870
Fragments arranged by size:
2037 1199 870
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 1731 1824 2880 1731
Size: 93 1056 2957
Fragments arranged by size:
2957 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 982 457
Size: 525 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 922 1282 2161 2653 2951 146
Size: 776 360 879 492 298 1301
Fragments arranged by size:
1301 879 776 492 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 160 958 3810 160
Size: 798 2852 456
Fragments arranged by size:
2852 798 456
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 814 844 814
Size: 30 4076
BseYI C'CCAG_C
Cuts at: 138 1531 2289 2866 3362 138
Size: 1393 758 577 496 882
Fragments arranged by size:
1393 882 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 4106
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1079 1120 1898 2088 2880 1079
Size: 41 778 190 792 2305
Fragments arranged by size:
2305 792 778 190 41
BsmI GAATG_Cn'
Cuts at: 620 620
Size: 4106
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 1079 1120 1731 1824 1898 2088 2150 2880 1079
Size: 41 611 93 74 190 62 730 2305
Fragments arranged by size:
2305 730 611 190 93 74 62 41
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 203 3282 203
Size: 3079 1027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 956 1156 1370 1672 2053 264
Size: 692 200 214 302 381 2317
Fragments arranged by size:
2317 692 381 302 214 200
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BsrGI T'GTAC_A
Cuts at: 599 768 599
Size: 169 3937
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 684 684
Size: 4106
BstYI r'GATC_y
Cuts at: 1 422 554 1131 1598 1621 1956 2202 3214
Size: 421 132 577 467 23 335 246 1012
Cuts at: 3214 3300 3312 3658 1
Size: 86 12 346 449
Fragments arranged by size:
1012 577 467 449 421 346 335 246
132 86 23 12
BstZ17I GTA'TAC
Cuts at: 194 194
Size: 4106
BtgI C'CryG_G
Cuts at: 107 107
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 199 2056 2237 2400 199
Size: 1857 181 163 1905
Fragments arranged by size:
1905 1857 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
ClaI AT'CG_AT
Cuts at: 912 912
Size: 4106
DraI TTT'AAA
Cuts at: 486 1105 486
Size: 619 3487
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 1691 1865 2256 2283 3628 1691
Size: 174 391 27 1345 2169
Fragments arranged by size:
2169 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 540 683 2129 2339 540
Size: 143 1446 210 2307
Fragments arranged by size:
2307 1446 210 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 457 646 982 1931 2363 3110 3378 110
Size: 347 189 336 949 432 747 268 838
Fragments arranged by size:
949 838 747 432 347 336 268 189
EcoO109I rG'GnC_Cy
Cuts at: 1068 1245 1068
Size: 177 3929
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 517 549 568 600 658 690 814 846 1948
Size: 32 19 32 58 32 124 32 1102
Cuts at: 1948 1978 1980 2010 517
Size: 30 2 30 2613
Fragments arranged by size:
2613 1102 124 58 32 32 32 32
30 30 19 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
HindIII A'AGCT_T
Cuts at: 284 284
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 203 2777 2961 203
Size: 2574 184 1348
Fragments arranged by size:
2574 1348 184
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 142 1217 1531 1891 2904 3149 142
Size: 1075 314 360 1013 245 1099
Fragments arranged by size:
1099 1075 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 363 1159 1659 2566 363
Size: 796 500 907 1903
Fragments arranged by size:
1903 907 796 500
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PfoI T'CCnGG_A
Cuts at: 473 473
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PstI C_TGCA'G
Cuts at: 275 1067 1153 1838 275
Size: 792 86 685 2543
Fragments arranged by size:
2543 792 685 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 142 1531 1891 142
Size: 1389 360 2357
Fragments arranged by size:
2357 1389 360
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 683 2129 2339 683
Size: 1446 210 2450
Fragments arranged by size:
2450 1446 210
SbfI CC_TGCA'GG
Cuts at: 275 1067 1153 275
Size: 792 86 3228
Fragments arranged by size:
3228 792 86
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 271 694 1063 1149 1834 2827 3018 33
Size: 238 423 369 86 685 993 191 1121
Fragments arranged by size:
1121 993 685 423 369 238 191 86
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 161 937 1261 2176 2668 2930 161
Size: 776 324 915 492 262 1337
Fragments arranged by size:
1337 915 776 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 363 1159 1659 363
Size: 796 500 2810
Fragments arranged by size:
2810 796 500
SspI AAT'ATT
Cuts at: 717 717
Size: 4106
StyI C'CwwG_G
Cuts at: 107 413 1240 1594 107
Size: 306 827 354 2619
Fragments arranged by size:
2619 827 354 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 625 1689 1822 2281 2490 625
Size: 1064 133 459 209 2241
Fragments arranged by size:
2241 1064 459 209 133
TatI w'GTAC_w
Cuts at: 599 768 3621 599
Size: 169 2853 1084
Fragments arranged by size:
2853 1084 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 720 1601 720
Size: 881 3225
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BssHII BstEII Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI