Restriction map for
pF1KB5403
(Circular) (Six-base) MAPSORT of: pF1KB5403.seq Check: 6952 from: 1 to: 5852
pF1KB5403 5852 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2138 2138
Size: 5852
AccI GT'mk_AC
Cuts at: 2162 2890 2162
Size: 728 5124
Acc65I G'GTAC_C
Cuts at: 1853 2868 1853
Size: 1015 4837
AclI AA'CG_TT
Cuts at: 5692 5692
Size: 5852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 3677 4109 4462 5124 635
Size: 3042 432 353 662 1363
Fragments arranged by size:
3042 1363 662 432 353
AfeI AGC'GCT
Cuts at: 1570 1570
Size: 5852
AflIII A'CryG_T
Cuts at: 1804 2313 2534 3204 5004 5285 1804
Size: 509 221 670 1800 281 2371
Fragments arranged by size:
2371 1800 670 509 281 221
AgeI A'CCGG_T
Cuts at: 127 127
Size: 5852
AhdI GACnn_n'nnGTC
Cuts at: 141 2016 141
Size: 1875 3977
AleI CACnn'nnGTG
Cuts at: 1945 1945
Size: 5852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1633 1963 1633
Size: 330 5522
AlwNI CAG_nnn'CTG
Cuts at: 1419 2055 2283 2670 3157 4595 1419
Size: 636 228 387 487 1438 2676
Fragments arranged by size:
2676 1438 636 487 387 228
ApaLI G'TGCA_C
Cuts at: 2560 4690 2560
Size: 2130 3722
ApoI r'AATT_y
Cuts at: 1468 2856 5205 5461 1468
Size: 1388 2349 256 1859
Fragments arranged by size:
2349 1859 1388 256
AseI AT'TA_AT
Cuts at: 20 2588 20
Size: 2568 3284
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
AvaI C'yCGr_G
Cuts at: 595 2416 2872 595
Size: 1821 456 3575
Fragments arranged by size:
3575 1821 456
BamHI G'GATC_C
Cuts at: 1796 2877 1796
Size: 1081 4771
BanI G'GyrC_C
Cuts at: 885 1853 2108 2743 2868 3282 3530 3565 885
Size: 968 255 635 125 414 248 35 3172
Fragments arranged by size:
3172 968 635 414 255 248 125 35
BanII G_rGCy'C
Cuts at: 173 362 1693 2654 2866 3896 173
Size: 189 1331 961 212 1030 2129
Fragments arranged by size:
2129 1331 1030 961 212 189
BbeI G_GCGC'C
Cuts at: 2112 3286 3534 2112
Size: 1174 248 4430
Fragments arranged by size:
4430 1174 248
BbsI GAAGACnn'nnnn_
Cuts at: 800 2362 800
Size: 1562 4290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 620 586
Size: 34 5818
BciVI GTATCCnnnnn_n'
Cuts at: 3750 4806 5852 3750
Size: 1056 1046 3750
Fragments arranged by size:
3750 1056 1046
BclI T'GATC_A
Cuts at: 2234 2582 3372 2234
Size: 348 790 4714
Fragments arranged by size:
4714 790 348
BglI GCCn_nnn'nGGC
Cuts at: 2332 2813 2332
Size: 481 5371
BglII A'GATC_T
Cuts at: 3367 5404 3367
Size: 2037 3815
BlpI GC'TnA_GC
Cuts at: 220 2964 220
Size: 2744 3108
Bme1580I G_kGCm'C
Cuts at: 1591 2564 2746 3477 3570 4694 1591
Size: 973 182 731 93 1124 2749
Fragments arranged by size:
2749 1124 973 731 182 93
BmrI ACTGGGnnnn_n'
Cuts at: 807 3064 3145 3480 5624 807
Size: 2257 81 335 2144 1035
Fragments arranged by size:
2257 2144 1035 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1710 4219 1710
Size: 2509 3343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2164 2173 2206 1597
Size: 567 9 33 5243
Fragments arranged by size:
5243 567 33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 3028 3907 4621 4919 852
Size: 2176 879 714 298 1785
Fragments arranged by size:
2176 1785 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2759 5556 2759
Size: 2797 3055
BsaAI yAC'GTr
Cuts at: 1515 3205 1515
Size: 1690 4162
BsaBI GATnn'nnATC
Cuts at: 6 1336 3366 5403 6
Size: 1330 2030 2037 455
Fragments arranged by size:
2037 2030 1330 455
BsaHI Gr'CG_yC
Cuts at: 1214 2109 3283 3531 4233 5729 1214
Size: 895 1174 248 702 1496 1337
Fragments arranged by size:
1496 1337 1174 895 702 248
BsaWI w'CCGG_w
Cuts at: 127 299 1793 3041 3562 4240 4651 4798 5072
Size: 172 1494 1248 521 678 411 147 274
Cuts at: 5072 127
Size: 907
Fragments arranged by size:
1494 1248 907 678 521 411 274 172
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1199 1229 1486 1516 1744 1774 2512 2542 1199
Size: 30 257 30 228 30 738 30 4509
Fragments arranged by size:
4509 738 257 228 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 295 646 850 994 997 1000 1594 1612
Size: 114 351 204 144 3 3 594 18
Cuts at: 1612 1683 2608 181
Size: 71 925 3425
Fragments arranged by size:
3425 925 594 351 204 144 114 71
18 3 3
BseYI C'CCAG_C
Cuts at: 1953 2286 2660 2694 3277 4035 4700 5108 1953
Size: 333 374 34 583 758 665 408 2697
Fragments arranged by size:
2697 758 665 583 408 374 333 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 1585 1615 1825 1942 1969 2116 1384
Size: 201 30 210 117 27 147 5120
Fragments arranged by size:
5120 210 201 147 117 30 27
BsiEI CG_ry'CG
Cuts at: 104 3394 3440 4670 104
Size: 3290 46 1230 1286
Fragments arranged by size:
3290 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 173 362 526 1523 2564 2866 3644 3834 4694
Size: 189 164 997 1041 302 778 190 860
Cuts at: 4694 173
Size: 1331
Fragments arranged by size:
1331 1041 997 860 778 302 190 189
164
BsmBI CGTCTCn'nnnn_
Cuts at: 2515 2515
Size: 5852
Bsp1286I G_dGCh'C
Cuts at: 173 362 526 1523 1591 1693 2564 2654 2746
Size: 189 164 997 68 102 871 90 92
Cuts at: 2746 2866 3477 3570 3644 3834 3896 4694 173
Size: 120 611 93 74 190 62 798 1331
Fragments arranged by size:
1331 997 871 798 611 190 189 164
120 102 93 92 90 74 68 62
BspEI T'CCGG_A
Cuts at: 299 299
Size: 5852
BspHI T'CATG_A
Cuts at: 1877 4284 1877
Size: 2407 3445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1406 1625 2138 2902 3116 3418 3799 1406
Size: 219 513 764 214 302 381 3459
Fragments arranged by size:
3459 764 513 381 302 219 214
BsrBI CCG'CTC
Cuts at: 50 2106 4144 4198 5682 5847 50
Size: 2056 2038 54 1484 165 55
Fragments arranged by size:
2056 2038 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3764 3764
Size: 5852
BsrFI r'CCGG_y
Cuts at: 127 3850 4031 127
Size: 3723 181 1948
Fragments arranged by size:
3723 1948 181
BssSI C'ACGA_G
Cuts at: 1124 4123 4831 1124
Size: 2999 708 2145
Fragments arranged by size:
2999 2145 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3229 3229
Size: 5852
BstBI TT'CG_AA
Cuts at: 4213 4213
Size: 5852
BstXI CCAn_nnnn'nTGG
Cuts at: 345 972 1298 2023 2184 2343 2517 345
Size: 627 326 725 161 159 174 3680
Fragments arranged by size:
3680 725 627 326 174 161 159
BstYI r'GATC_y
Cuts at: 1 539 1112 1796 1925 2189 2453 2547 2877
Size: 538 573 684 129 264 264 94 330
Cuts at: 2877 3344 3367 3702 3948 4254 4266 4352 5404
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5404 1
Size: 449
Fragments arranged by size:
1052 684 573 538 467 449 335 330
306 264 264 246 129 94 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1685 1685
Size: 5852
BtgI C'CryG_G
Cuts at: 1428 1943 2808 1428
Size: 515 865 4472
Fragments arranged by size:
4472 865 515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1227 2231 3802 3983 4146 1227
Size: 1004 1571 181 163 2933
Fragments arranged by size:
2933 1571 1004 181 163
BtsI GCAGTG_nn'
Cuts at: 264 1417 2502 2654 3095 264
Size: 1153 1085 152 441 3021
Fragments arranged by size:
3021 1153 1085 441 152
DraI TTT'AAA
Cuts at: 2851 2851
Size: 5852
DraIII CAC_nnn'GTG
Cuts at: 341 1423 341
Size: 1082 4770
DrdI GACnn_nn'nnGTC
Cuts at: 3558 4902 3558
Size: 1344 4508
EaeI y'GGCC_r
Cuts at: 2025 2201 2684 3437 3611 4002 4029 5374 2025
Size: 176 483 753 174 391 27 1345 2503
Fragments arranged by size:
2503 1345 753 483 391 176 174 27
EagI C'GGCC_G
Cuts at: 3437 3437
Size: 5852
EarI CTCTTCn'nnn_
Cuts at: 344 654 1379 2456 2488 3875 4085 344
Size: 310 725 1077 32 1387 210 2111
Fragments arranged by size:
2111 1387 1077 725 310 210 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1040 1960 4060 4802 4948 5662 1040
Size: 920 2100 742 146 714 1230
Fragments arranged by size:
2100 1230 920 742 714 146
EcoICRI GAG'CTC
Cuts at: 171 360 2864 171
Size: 189 2504 3159
Fragments arranged by size:
3159 2504 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 1597 2164 2173 2206 3677 4109 4462 5124
Size: 962 567 9 33 1471 432 353 662
Cuts at: 5124 635
Size: 1363
Fragments arranged by size:
1471 1363 962 662 567 432 353 33
9
EcoNI CCTnn'n_nnAGG
Cuts at: 188 976 1207 1984 188
Size: 788 231 777 4056
Fragments arranged by size:
4056 788 777 231
EcoO109I rG'GnC_Cy
Cuts at: 2052 2118 2290 2406 2474 2991 2052
Size: 66 172 116 68 517 4913
Fragments arranged by size:
4913 517 172 116 68 66
EcoRI G'AATT_C
Cuts at: 2856 2856
Size: 5852
EcoRV GAT'ATC
Cuts at: 1338 1338
Size: 5852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3871 3871
Size: 5852
FspI TGC'GCA
Cuts at: 1624 2065 3633 1624
Size: 441 1568 3843
Fragments arranged by size:
3843 1568 441
HaeII r_GCGC'y
Cuts at: 972 1572 2112 3286 3534 4764 5535 972
Size: 600 540 1174 248 1230 771 1289
Fragments arranged by size:
1289 1230 1174 771 600 540 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 133 159 165 550 582 754 786 814
Size: 6 26 6 385 32 172 32 28
Cuts at: 814 846 946 978 1199 1231 1486 1518 2002
Size: 32 100 32 221 32 255 32 484
Cuts at: 2002 2034 2219 2251 2512 2544 3694 3724 3726
Size: 32 185 32 261 32 1150 30 2
Cuts at: 3726 3756 127
Size: 30 2223
Fragments arranged by size:
2223 1150 484 385 261 255 221 185
172 100 32 32 32 32 32 32
32 32 32 30 30 28 26 6
6 2
HincII GTy'rAC
Cuts at: 2891 2891
Size: 5852
KasI G'GCGC_C
Cuts at: 2108 3282 3530 2108
Size: 1174 248 4430
Fragments arranged by size:
4430 1174 248
KpnI G_GTAC'C
Cuts at: 1857 2872 1857
Size: 1015 4837
MluI A'CGCG_T
Cuts at: 5285 5285
Size: 5852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 168 851 1347 4611 4795 168
Size: 683 496 3264 184 1225
Fragments arranged by size:
3264 1225 683 496 184
MscI TGG'CCA
Cuts at: 3613 3613
Size: 5852
MslI CAynn'nnrTG
Cuts at: 1876 1945 2341 2515 3841 3968 1876
Size: 69 396 174 1326 127 3760
Fragments arranged by size:
3760 1326 396 174 127 69
MspA1I CmG'CkG
Cuts at: 1192 1674 1953 2259 2682 2810 2963 3277 3637
Size: 482 279 306 423 128 153 314 360
Cuts at: 3637 4421 4666 1192
Size: 784 245 2378
Fragments arranged by size:
2378 784 482 423 360 314 306 279
245 153 128
NaeI GCC'GGC
Cuts at: 4033 4033
Size: 5852
NarI GG'CG_CC
Cuts at: 2109 3283 3531 2109
Size: 1174 248 4430
Fragments arranged by size:
4430 1174 248
NcoI C'CATG_G
Cuts at: 1943 1943
Size: 5852
NdeI CA'TA_TG
Cuts at: 375 375
Size: 5852
NgoMIV G'CCGG_C
Cuts at: 4031 4031
Size: 5852
NspI r_CATG'y
Cuts at: 1808 1909 2317 2538 2805 2905 3405 5008 1808
Size: 101 408 221 267 100 500 1603 2652
Fragments arranged by size:
2652 1603 500 408 267 221 101 100
PciI A'CATG_T
Cuts at: 1804 2313 2534 5004 1804
Size: 509 221 2470 2652
Fragments arranged by size:
2652 2470 509 221
PfoI T'CCnGG_A
Cuts at: 2183 2456 2183
Size: 273 5579
PmeI GTTT'AAAC
Cuts at: 2851 2851
Size: 5852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 4298
Size: 32 5820
PpuMI rG'GwC_Cy
Cuts at: 2118 2290 2406 2474 2118
Size: 172 116 68 5496
Fragments arranged by size:
5496 172 116 68
PstI C_TGCA'G
Cuts at: 2075 2681 2899 3584 2075
Size: 606 218 685 4343
Fragments arranged by size:
4343 685 606 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5852
PvuII CAG'CTG
Cuts at: 1674 1953 2259 2682 3277 3637 1674
Size: 279 306 423 595 360 3889
Fragments arranged by size:
3889 595 423 360 306 279
RsrII CG'GwC_CG
Cuts at: 4047 4047
Size: 5852
SacI G_AGCT'C
Cuts at: 173 362 2866 173
Size: 189 2504 3159
Fragments arranged by size:
3159 2504 189
SacII CC_GC'GG
Cuts at: 2811 2811
Size: 5852
SalI G'TCGA_C
Cuts at: 2889 2889
Size: 5852
SanDI GG'GwC_CC
Cuts at: 2290 2290
Size: 5852
SapI GCTCTTCn'nnn_
Cuts at: 654 3875 4085 654
Size: 3221 210 2421
Fragments arranged by size:
3221 2421 210
SbfI CC_TGCA'GG
Cuts at: 2075 2899 2075
Size: 824 5028
ScaI AGT'ACT
Cuts at: 5369 5369
Size: 5852
SexAI A'CCwGG_T
Cuts at: 2165 2165
Size: 5852
SfcI C'TryA_G
Cuts at: 33 1117 2071 2490 2677 2895 3580 4548 4739
Size: 1084 954 419 187 218 685 968 191
Cuts at: 4739 33
Size: 1146
Fragments arranged by size:
1146 1084 968 954 685 419 218 191
187
SfoI GGC'GCC
Cuts at: 2110 3284 3532 2110
Size: 1174 248 4430
Fragments arranged by size:
4430 1174 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5852
SmaI CCC'GGG
Cuts at: 2874 2874
Size: 5852
SmlI C'TyrA_G
Cuts at: 867 3007 3922 4636 4898 867
Size: 2140 915 714 262 1821
Fragments arranged by size:
2140 1821 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5852
SphI G_CATG'C
Cuts at: 1909 2905 3405 1909
Size: 996 500 4356
Fragments arranged by size:
4356 996 500
SspI AAT'ATT
Cuts at: 1377 1377
Size: 5852
StuI AGG'CCT
Cuts at: 1979 1979
Size: 5852
StyI C'CwwG_G
Cuts at: 263 512 1064 1778 1943 1974 1985 2033 2330
Size: 249 552 714 165 31 11 48 297
Cuts at: 2330 2986 3340 263
Size: 656 354 2775
Fragments arranged by size:
2775 714 656 552 354 297 249 165
48 31 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 1096 2335 3435 3568 4027 4236 903
Size: 193 1239 1100 133 459 209 2519
Fragments arranged by size:
2519 1239 1100 459 209 193 133
TatI w'GTAC_w
Cuts at: 1766 5367 1766
Size: 3601 2251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1409 1441 1744 1776 5369 5401 1409
Size: 32 303 32 3593 32 1860
Fragments arranged by size:
3593 1860 303 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 676 2428 2526 3649 676
Size: 1752 98 1123 2879
Fragments arranged by size:
2879 1752 1123 98
XbaI T'CTAG_A
Cuts at: 2883 2883
Size: 5852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 1950 2040 3347 393
Size: 1557 90 1307 2898
Fragments arranged by size:
2898 1557 1307 90
XmaI C'CCGG_G
Cuts at: 2872 2872
Size: 5852
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI