Restriction map for
pF1KB5401
(Circular) (Six-base) MAPSORT of: pF1KB5401.seq Check: 7470 from: 1 to: 3902
pF1KB5401 3902 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 483 483
Size: 3902
AccI GT'mk_AC
Cuts at: 940 940
Size: 3902
Acc65I G'GTAC_C
Cuts at: 918 918
Size: 3902
AclI AA'CG_TT
Cuts at: 3742 3742
Size: 3902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1727 2159 2906 3174 488
Size: 1239 432 747 268 1216
Fragments arranged by size:
1239 1216 747 432 268
AflIII A'CryG_T
Cuts at: 1254 2358 3335 1254
Size: 1104 977 1821
Fragments arranged by size:
1821 1104 977
AgeI A'CCGG_T
Cuts at: 2290 2290
Size: 3902
AlwNI CAG_nnn'CTG
Cuts at: 314 432 1207 2774 314
Size: 118 775 1567 1442
Fragments arranged by size:
1567 1442 775 118
ApaI G_GGCC'C
Cuts at: 347 347
Size: 3902
ApaLI G'TGCA_C
Cuts at: 2672 2672
Size: 3902
ApoI r'AATT_y
Cuts at: 906 3255 3511 906
Size: 2349 256 1297
Fragments arranged by size:
2349 1297 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3902
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
AvaI C'yCGr_G
Cuts at: 922 922
Size: 3902
BamHI G'GATC_C
Cuts at: 927 927
Size: 3902
BanI G'GyrC_C
Cuts at: 918 1332 1580 1615 918
Size: 414 248 35 3205
Fragments arranged by size:
3205 414 248 35
BanII G_rGCy'C
Cuts at: 347 916 1946 347
Size: 569 1030 2303
Fragments arranged by size:
2303 1030 569
BbeI G_GCGC'C
Cuts at: 1336 1584 1336
Size: 248 3654
BbsI GAAGACnn'nnnn_
Cuts at: 814 814
Size: 3902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3868
BciVI GTATCCnnnnn_n'
Cuts at: 1800 2561 3902 1800
Size: 761 1341 1800
Fragments arranged by size:
1800 1341 761
BclI T'GATC_A
Cuts at: 644 720 1422 644
Size: 76 702 3124
Fragments arranged by size:
3124 702 76
BglII A'GATC_T
Cuts at: 1417 3454 1417
Size: 2037 1865
BlpI GC'TnA_GC
Cuts at: 1014 1014
Size: 3902
Bme1580I G_kGCm'C
Cuts at: 347 1527 1620 2676 347
Size: 1180 93 1056 1573
Fragments arranged by size:
1573 1180 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1114 1195 1530 3674 1114
Size: 81 335 2144 1342
Fragments arranged by size:
2144 1342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 438 2269 438
Size: 1831 2071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 821 194
Size: 627 3275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1957 2449 2747 1078
Size: 879 492 298 2233
Fragments arranged by size:
2233 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 409 3606 409
Size: 3197 705
BsaAI yAC'GTr
Cuts at: 621 1255 621
Size: 634 3268
BsaBI GATnn'nnATC
Cuts at: 6 559 643 1416 3453 6
Size: 553 84 773 2037 455
Fragments arranged by size:
2037 773 553 455 84
BsaHI Gr'CG_yC
Cuts at: 374 1333 1581 2283 3779 374
Size: 959 248 702 1496 497
Fragments arranged by size:
1496 959 702 497 248
BsaWI w'CCGG_w
Cuts at: 1091 1612 2290 2564 2711 3122 1091
Size: 521 678 274 147 411 1871
Fragments arranged by size:
1871 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 205
Size: 30 3872
BseYI C'CCAG_C
Cuts at: 624 1327 2085 2662 3158 624
Size: 703 758 577 496 1368
Fragments arranged by size:
1368 758 703 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 511 565 500
Size: 11 54 3837
Fragments arranged by size:
3837 54 11
BsiEI CG_ry'CG
Cuts at: 104 1444 1490 2698 104
Size: 1340 46 1208 1308
Fragments arranged by size:
1340 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 916 1694 1884 2676 916
Size: 778 190 792 2142
Fragments arranged by size:
2142 792 778 190
BsmI GAATG_Cn'
Cuts at: 130 130
Size: 3902
Bsp1286I G_dGCh'C
Cuts at: 347 916 1527 1620 1694 1884 1946 2676 347
Size: 569 611 93 74 190 62 730 1573
Fragments arranged by size:
1573 730 611 569 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3122 3122
Size: 3902
BspHI T'CATG_A
Cuts at: 3078 3078
Size: 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 483 952 1166 1468 1849 410
Size: 73 469 214 302 381 2463
Fragments arranged by size:
2463 469 381 302 214 73
BsrBI CCG'CTC
Cuts at: 50 269 523 2194 2248 3732 3897 50
Size: 219 254 1671 54 1484 165 55
Fragments arranged by size:
1671 1484 254 219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 650 1814 650
Size: 1164 2738
BsrFI r'CCGG_y
Cuts at: 298 370 1900 2081 2290 298
Size: 72 1530 181 209 1910
Fragments arranged by size:
1910 1530 209 181 72
BssSI C'ACGA_G
Cuts at: 2173 2531 2173
Size: 358 3544
BstAPI GCAn_nnn'nTGC
Cuts at: 1279 1279
Size: 3902
BstBI TT'CG_AA
Cuts at: 2263 2263
Size: 3902
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3902
BstYI r'GATC_y
Cuts at: 1 169 927 1394 1417 1752 1998 3010 3096
Size: 168 758 467 23 335 246 1012 86
Cuts at: 3096 3108 3454 1
Size: 12 346 449
Fragments arranged by size:
1012 758 467 449 346 335 246 168
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 848 848
Size: 3902
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1852 2033 2196 1852
Size: 181 163 3558
Fragments arranged by size:
3558 181 163
BtsI GCAGTG_nn'
Cuts at: 1145 1145
Size: 3902
DraI TTT'AAA
Cuts at: 901 901
Size: 3902
DrdI GACnn_nn'nnGTC
Cuts at: 1608 2466 1608
Size: 858 3044
EaeI y'GGCC_r
Cuts at: 195 1487 1661 2052 2079 3424 195
Size: 1292 174 391 27 1345 673
Fragments arranged by size:
1345 1292 673 391 174 27
EagI C'GGCC_G
Cuts at: 1487 1487
Size: 3902
EarI CTCTTCn'nnn_
Cuts at: 1925 2135 1925
Size: 210 3692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2110 2420 2566 3712 2110
Size: 310 146 1146 2300
Fragments arranged by size:
2300 1146 310 146
EcoICRI GAG'CTC
Cuts at: 914 914
Size: 3902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 488 821 1727 2159 2906 3174 194
Size: 294 333 906 432 747 268 922
Fragments arranged by size:
922 906 747 432 333 294 268
EcoNI CCTnn'n_nnAGG
Cuts at: 690 690
Size: 3902
EcoO109I rG'GnC_Cy
Cuts at: 333 344 399 429 600 1041 333
Size: 11 55 30 171 441 3194
Fragments arranged by size:
3194 441 171 55 30 11
EcoRI G'AATT_C
Cuts at: 906 906
Size: 3902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3902
FspI TGC'GCA
Cuts at: 1683 1683
Size: 3902
HaeII r_GCGC'y
Cuts at: 1336 1584 2606 3585 1336
Size: 248 1022 979 1653
Fragments arranged by size:
1653 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 653 685 1744 1774 1776 1806 205
Size: 32 416 32 1059 30 2 30 2301
Fragments arranged by size:
2301 1059 416 32 32 30 30 2
HincII GTy'rAC
Cuts at: 843 941 843
Size: 98 3804
KasI G'GCGC_C
Cuts at: 1332 1580 1332
Size: 248 3654
KpnI G_GTAC'C
Cuts at: 922 922
Size: 3902
MluI A'CGCG_T
Cuts at: 3335 3335
Size: 3902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2757 2573
Size: 184 3718
MscI TGG'CCA
Cuts at: 1663 1663
Size: 3902
MslI CAynn'nnrTG
Cuts at: 634 640 1891 2018 634
Size: 6 1251 127 2518
Fragments arranged by size:
2518 1251 127 6
MspA1I CmG'CkG
Cuts at: 242 541 570 1013 1327 1687 2700 2945 242
Size: 299 29 443 314 360 1013 245 1199
Fragments arranged by size:
1199 1013 443 360 314 299 245 29
NaeI GCC'GGC
Cuts at: 300 372 2083 300
Size: 72 1711 2119
Fragments arranged by size:
2119 1711 72
NarI GG'CG_CC
Cuts at: 1333 1581 1333
Size: 248 3654
NgoMIV G'CCGG_C
Cuts at: 298 370 2081 298
Size: 72 1711 2119
Fragments arranged by size:
2119 1711 72
NspI r_CATG'y
Cuts at: 150 955 1455 2362 150
Size: 805 500 907 1690
Fragments arranged by size:
1690 907 805 500
PciI A'CATG_T
Cuts at: 2358 2358
Size: 3902
PflMI CCAn_nnn'nTGG
Cuts at: 556 556
Size: 3902
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 3902
PmeI GTTT'AAAC
Cuts at: 901 901
Size: 3902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3870
PpuMI rG'GwC_Cy
Cuts at: 333 399 600 333
Size: 66 201 3635
Fragments arranged by size:
3635 201 66
PspOMI G'GGCC_C
Cuts at: 343 343
Size: 3902
PstI C_TGCA'G
Cuts at: 949 1634 949
Size: 685 3217
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3902
PvuII CAG'CTG
Cuts at: 541 1327 1687 541
Size: 786 360 2756
Fragments arranged by size:
2756 786 360
RsrII CG'GwC_CG
Cuts at: 2097 2097
Size: 3902
SacI G_AGCT'C
Cuts at: 916 916
Size: 3902
SalI G'TCGA_C
Cuts at: 939 939
Size: 3902
SapI GCTCTTCn'nnn_
Cuts at: 1925 2135 1925
Size: 210 3692
SbfI CC_TGCA'GG
Cuts at: 949 949
Size: 3902
ScaI AGT'ACT
Cuts at: 3419 3419
Size: 3902
SfcI C'TryA_G
Cuts at: 33 945 1630 2623 2814 33
Size: 912 685 993 191 1121
Fragments arranged by size:
1121 993 912 685 191
SfoI GGC'GCC
Cuts at: 1334 1582 1334
Size: 248 3654
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
SmaI CCC'GGG
Cuts at: 924 924
Size: 3902
SmlI C'TyrA_G
Cuts at: 1057 1972 2464 2726 1057
Size: 915 492 262 2233
Fragments arranged by size:
2233 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3902
SphI G_CATG'C
Cuts at: 955 1455 955
Size: 500 3402
StyI C'CwwG_G
Cuts at: 587 1036 1390 587
Size: 449 354 3099
Fragments arranged by size:
3099 449 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1485 1618 2077 2286 1485
Size: 133 459 209 3101
Fragments arranged by size:
3101 459 209 133
TatI w'GTAC_w
Cuts at: 3417 3417
Size: 3902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 3870
Tth111I GACn'n_nGTC
Cuts at: 1699 1699
Size: 3902
XbaI T'CTAG_A
Cuts at: 933 933
Size: 3902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1397 1397
Size: 3902
XmaI C'CCGG_G
Cuts at: 922 922
Size: 3902
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI