Restriction map for
pF1KB5398
(Circular) (Six-base) MAPSORT of: pF1KB5398.seq Check: 9116 from: 1 to: 3656
pF1KB5398 3656 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 589 589
Size: 3656
AccI GT'mk_AC
Cuts at: 694 694
Size: 3656
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2266 2928 1481
Size: 432 353 662 2209
Fragments arranged by size:
2209 662 432 353
AflII C'TTAA_G
Cuts at: 217 217
Size: 3656
AflIII A'CryG_T
Cuts at: 1008 2808 3089 1008
Size: 1800 281 1575
Fragments arranged by size:
1800 1575 281
AhdI GACnn_n'nnGTC
Cuts at: 624 624
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 563 961 2399 563
Size: 398 1438 1820
Fragments arranged by size:
1820 1438 398
ApaLI G'TGCA_C
Cuts at: 2494 2494
Size: 3656
ApoI r'AATT_y
Cuts at: 660 3009 3265 660
Size: 2349 256 1051
Fragments arranged by size:
2349 1051 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 676 676
Size: 3656
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 341 672 1086 1334 1369 341
Size: 331 414 248 35 2628
Fragments arranged by size:
2628 414 331 248 35
BanII G_rGCy'C
Cuts at: 428 455 605 670 1700 428
Size: 27 150 65 1030 2384
Fragments arranged by size:
2384 1030 150 65 27
BbeI G_GCGC'C
Cuts at: 345 1090 1338 345
Size: 745 248 2663
Fragments arranged by size:
2663 745 248
BbsI GAAGACnn'nnnn_
Cuts at: 202 202
Size: 3656
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2610 3656 1554
Size: 1056 1046 1554
Fragments arranged by size:
1554 1056 1046
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglI GCCn_nnn'nGGC
Cuts at: 339 339
Size: 3656
BglII A'GATC_T
Cuts at: 1171 3208 1171
Size: 2037 1619
BlpI GC'TnA_GC
Cuts at: 768 768
Size: 3656
Bme1580I G_kGCm'C
Cuts at: 1281 1374 2498 1281
Size: 93 1124 2439
Fragments arranged by size:
2439 1124 93
BmgBI CAC'GTC
Cuts at: 309 309
Size: 3656
BmrI ACTGGGnnnn_n'
Cuts at: 868 949 1284 3428 868
Size: 81 335 2144 1096
Fragments arranged by size:
2144 1096 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1711 2425 2723 832
Size: 879 714 298 1765
Fragments arranged by size:
1765 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3360 3360
Size: 3656
BsaAI yAC'GTr
Cuts at: 1009 1009
Size: 3656
BsaBI GATnn'nnATC
Cuts at: 6 1170 3207 6
Size: 1164 2037 455
Fragments arranged by size:
2037 1164 455
BsaHI Gr'CG_yC
Cuts at: 342 1087 1335 2037 3533 342
Size: 745 248 702 1496 465
Fragments arranged by size:
1496 745 702 465 248
BsaWI w'CCGG_w
Cuts at: 638 845 1366 2044 2455 2602 2876 638
Size: 207 521 678 411 147 274 1418
Fragments arranged by size:
1418 678 521 411 274 207 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 436
Size: 3656
BseYI C'CCAG_C
Cuts at: 222 534 1081 1839 2504 2912 222
Size: 312 547 758 665 408 966
Fragments arranged by size:
966 758 665 547 408 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 526
Size: 3656
BsiEI CG_ry'CG
Cuts at: 104 518 1198 1244 2474 104
Size: 414 680 46 1230 1286
Fragments arranged by size:
1286 1230 680 414 46
BsiHKAI G_wGCw'C
Cuts at: 428 455 670 1448 1638 2498 428
Size: 27 215 778 190 860 1586
Fragments arranged by size:
1586 860 778 215 190 27
BsmI GAATG_Cn'
Cuts at: 516 516
Size: 3656
Bsp1286I G_dGCh'C
Cuts at: 428 455 605 670 1281 1374 1448 1638 1700
Size: 27 150 65 611 93 74 190 62
Cuts at: 1700 2498 428
Size: 798 1586
Fragments arranged by size:
1586 798 611 190 150 93 74 65
62 27
BspEI T'CCGG_A
Cuts at: 638 638
Size: 3656
BspHI T'CATG_A
Cuts at: 428 2088 428
Size: 1660 1996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 589 706 920 1222 1603 589
Size: 117 214 302 381 2642
Fragments arranged by size:
2642 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 1948 2002 3486 3651 50
Size: 1898 54 1484 165 55
Fragments arranged by size:
1898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 1568 396
Size: 1172 2484
BsrFI r'CCGG_y
Cuts at: 1654 1835 1654
Size: 181 3475
BssSI C'ACGA_G
Cuts at: 1927 2635 1927
Size: 708 2948
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstYI r'GATC_y
Cuts at: 1 326 681 1148 1171 1506 1752 2058 2070
Size: 325 355 467 23 335 246 306 12
Cuts at: 2070 2156 3208 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 449 355 335 325 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 272 107
Size: 165 3491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1606 1787 1950 1606
Size: 181 163 3312
Fragments arranged by size:
3312 181 163
BtsI GCAGTG_nn'
Cuts at: 899 899
Size: 3656
DraI TTT'AAA
Cuts at: 655 655
Size: 3656
DraIII CAC_nnn'GTG
Cuts at: 302 302
Size: 3656
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2706 1362
Size: 1344 2312
EaeI y'GGCC_r
Cuts at: 315 440 515 1241 1415 1806 1833 3178 315
Size: 125 75 726 174 391 27 1345 793
Fragments arranged by size:
1345 793 726 391 174 125 75 27
EagI C'GGCC_G
Cuts at: 515 1241 515
Size: 726 2930
EarI CTCTTCn'nnn_
Cuts at: 136 1679 1889 136
Size: 1543 210 1903
Fragments arranged by size:
1903 1543 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1864 2606 2752 3466 1864
Size: 742 146 714 2054
Fragments arranged by size:
2054 742 714 146
EcoICRI GAG'CTC
Cuts at: 426 453 668 426
Size: 27 215 3414
Fragments arranged by size:
3414 215 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2266 2928 1481
Size: 432 353 662 2209
Fragments arranged by size:
2209 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 133 133
Size: 3656
EcoO109I rG'GnC_Cy
Cuts at: 138 492 795 138
Size: 354 303 2999
Fragments arranged by size:
2999 354 303
EcoRI G'AATT_C
Cuts at: 660 660
Size: 3656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 1675
Size: 3656
FspI TGC'GCA
Cuts at: 163 1437 163
Size: 1274 2382
FspAI rTGC'GCAy
Cuts at: 163 163
Size: 3656
HaeII r_GCGC'y
Cuts at: 345 1090 1338 2568 3339 345
Size: 745 248 1230 771 662
Fragments arranged by size:
1230 771 745 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 1498 1528 1530 1560 610
Size: 32 856 30 2 30 2706
Fragments arranged by size:
2706 856 32 30 30 2
HincII GTy'rAC
Cuts at: 695 695
Size: 3656
HindIII A'AGCT_T
Cuts at: 214 214
Size: 3656
KasI G'GCGC_C
Cuts at: 341 1086 1334 341
Size: 745 248 2663
Fragments arranged by size:
2663 745 248
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2415 2599 2415
Size: 184 3472
MscI TGG'CCA
Cuts at: 317 1417 317
Size: 1100 2556
MslI CAynn'nnrTG
Cuts at: 169 433 1645 1772 169
Size: 264 1212 127 2053
Fragments arranged by size:
2053 1212 264 127
MspA1I CmG'CkG
Cuts at: 593 767 1081 1441 2225 2470 593
Size: 174 314 360 784 245 1779
Fragments arranged by size:
1779 784 360 314 245 174
NaeI GCC'GGC
Cuts at: 1837 1837
Size: 3656
NarI GG'CG_CC
Cuts at: 342 1087 1335 342
Size: 745 248 2663
Fragments arranged by size:
2663 745 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3656
NgoMIV G'CCGG_C
Cuts at: 1835 1835
Size: 3656
NspI r_CATG'y
Cuts at: 709 1209 2812 709
Size: 500 1603 1553
Fragments arranged by size:
1603 1553 500
PciI A'CATG_T
Cuts at: 2808 2808
Size: 3656
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 3656
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2102 2134 2102
Size: 32 3624
PpuMI rG'GwC_Cy
Cuts at: 492 492
Size: 3656
PstI C_TGCA'G
Cuts at: 192 703 1388 192
Size: 511 685 2460
Fragments arranged by size:
2460 685 511
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 1081 1441 1081
Size: 360 3296
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 428 455 670 428
Size: 27 215 3414
Fragments arranged by size:
3414 215 27
SalI G'TCGA_C
Cuts at: 693 693
Size: 3656
SapI GCTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 3173 3173
Size: 3656
SfcI C'TryA_G
Cuts at: 33 188 699 1384 2352 2543 33
Size: 155 511 685 968 191 1146
Fragments arranged by size:
1146 968 685 511 191 155
SfoI GGC'GCC
Cuts at: 343 1088 1336 343
Size: 745 248 2663
Fragments arranged by size:
2663 745 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 678 678
Size: 3656
SmlI C'TyrA_G
Cuts at: 217 811 1726 2440 2702 217
Size: 594 915 714 262 1171
Fragments arranged by size:
1171 915 714 594 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
StuI AGG'CCT
Cuts at: 368 368
Size: 3656
StyI C'CwwG_G
Cuts at: 107 790 1144 107
Size: 683 354 2619
Fragments arranged by size:
2619 683 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 301 1239 1372 1831 2040 214
Size: 87 938 133 459 209 1830
Fragments arranged by size:
1830 938 459 209 133 87
TatI w'GTAC_w
Cuts at: 278 3171 278
Size: 2893 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 3624
Tth111I GACn'n_nGTC
Cuts at: 1453 1453
Size: 3656
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1151 1151
Size: 3656
XmaI C'CCGG_G
Cuts at: 676 676
Size: 3656
XmnI GAAnn'nnTTC
Cuts at: 118 118
Size: 3656
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI