Restriction map for
pF1KB5397
(Circular) (Six-base) MAPSORT of: pF1KB5397.seq Check: 1050 from: 1 to: 4040
pF1KB5397 4040 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 922 1865 2297 3044 3312 493
Size: 429 943 432 747 268 1221
Fragments arranged by size:
1221 943 747 432 429 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 165 1060 165
Size: 895 3145
BamHI G'GATC_C
Cuts at: 1000 1065 1000
Size: 65 3975
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 423 1054 2084 423
Size: 631 1030 2379
Fragments arranged by size:
2379 1030 631
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbvCI CC'TCA_GC
Cuts at: 121 121
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 556 1938 2699 4040 556
Size: 1382 761 1341 556
Fragments arranged by size:
1382 1341 761 556
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglI GCCn_nnn'nGGC
Cuts at: 427 427
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 320 487 1665 1758 2814 320
Size: 167 1178 93 1056 1546
Fragments arranged by size:
1546 1178 1056 167 93
BmgBI CAC'GTC
Cuts at: 546 554 546
Size: 8 4032
BmrI ACTGGGnnnn_n'
Cuts at: 606 1252 1333 1668 3812 606
Size: 646 81 335 2144 834
Fragments arranged by size:
2144 834 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 804 2407 804
Size: 1603 2437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 748 661
Size: 87 3953
Bpu10I CC'TnA_GC
Cuts at: 121 121
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 486 516 640 670 486
Size: 30 124 30 3856
Fragments arranged by size:
3856 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 512 127
Size: 385 3655
BseYI C'CCAG_C
Cuts at: 132 422 738 810 1465 2223 2800 3296 132
Size: 290 316 72 655 758 577 496 876
Fragments arranged by size:
876 758 655 577 496 316 290 72
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 377 1054 1832 2022 2814 377
Size: 677 778 190 792 1603
Fragments arranged by size:
1603 792 778 677 190
BsmI GAATG_Cn'
Cuts at: 623 623
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 320 377 423 487 1054 1665 1758 1832 2022
Size: 57 46 64 567 611 93 74 190
Cuts at: 2022 2084 2814 320
Size: 62 730 1546
Fragments arranged by size:
1546 730 611 567 190 93 74 64
62 57 46
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 1016 3216 1016
Size: 2200 1840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 795 2038 2219 2428 795
Size: 1243 181 209 2407
Fragments arranged by size:
2407 1243 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 704 1000 1065 1532 1555 1890 2136 3148
Size: 703 296 65 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 703 467 449 346 335 296 246
86 65 23 12
BtgI C'CryG_G
Cuts at: 107 155 750 897 107
Size: 48 595 147 3250
Fragments arranged by size:
3250 595 147 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 428 825 1625 1799 2190 2217 3562 428
Size: 397 800 174 391 27 1345 906
Fragments arranged by size:
1345 906 800 397 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 259 879 2248 2558 2704 3850 259
Size: 620 1369 310 146 1146 449
Fragments arranged by size:
1369 1146 620 449 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 661 748 922 1865 2297 3044 3312 493
Size: 168 87 174 943 432 747 268 1221
Fragments arranged by size:
1221 943 747 432 268 174 168 87
EcoNI CCTnn'n_nnAGG
Cuts at: 475 571 475
Size: 96 3944
EcoO109I rG'GnC_Cy
Cuts at: 672 756 915 1179 672
Size: 84 159 264 3533
Fragments arranged by size:
3533 264 159 84
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 936 1474 1722 2744 3723 936
Size: 538 248 1022 979 1253
Fragments arranged by size:
1253 1022 979 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 1882 1912 1914 1944 640
Size: 32 1210 30 2 30 2736
Fragments arranged by size:
2736 1210 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
HindIII A'AGCT_T
Cuts at: 412 454 412
Size: 42 3998
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 382 2029 2156 382
Size: 1647 127 2266
Fragments arranged by size:
2266 1647 127
MspA1I CmG'CkG
Cuts at: 426 1151 1465 1825 2838 3083 426
Size: 725 314 360 1013 245 1383
Fragments arranged by size:
1383 1013 725 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 861 1093 1593 2500 861
Size: 232 500 907 2401
Fragments arranged by size:
2401 907 500 232
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 756 756
Size: 4040
PstI C_TGCA'G
Cuts at: 142 698 1087 1772 142
Size: 556 389 685 2410
Fragments arranged by size:
2410 685 556 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 426 1465 1825 426
Size: 1039 360 2641
Fragments arranged by size:
2641 1039 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 138 694 1083 1768 2761 2952 33
Size: 105 556 389 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 389 191 105
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 107 239 911 1174 1528 107
Size: 132 672 263 354 2619
Fragments arranged by size:
2619 672 354 263 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 486 518 530 562 3557 3589 486
Size: 32 12 32 2995 32 937
Fragments arranged by size:
2995 937 32 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 598 1837 598
Size: 1239 2801
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 1535 333
Size: 1202 2838
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmtI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI