Restriction map for
pF1KB5389
(Circular) (Six-base) MAPSORT of: pF1KB5389.seq Check: 5084 from: 1 to: 4550
pF1KB5389 4550 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 986 1544 4390 986
Size: 558 2846 1146
Fragments arranged by size:
2846 1146 558
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1018 2375 2807 3160 3822 703
Size: 315 1357 432 353 662 1431
Fragments arranged by size:
1431 1357 662 432 353 315
AfeI AGC'GCT
Cuts at: 1240 1240
Size: 4550
AflII C'TTAA_G
Cuts at: 181 181
Size: 4550
AflIII A'CryG_T
Cuts at: 1280 1902 3702 3983 1280
Size: 622 1800 281 1847
Fragments arranged by size:
1847 1800 622 281
AleI CACnn'nnGTG
Cuts at: 1279 1279
Size: 4550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 167 167
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 908 918 1855 3293 908
Size: 10 937 1438 2165
Fragments arranged by size:
2165 1438 937 10
ApaI G_GGCC'C
Cuts at: 129 129
Size: 4550
ApaLI G'TGCA_C
Cuts at: 3388 3388
Size: 4550
ApoI r'AATT_y
Cuts at: 667 1554 3903 4159 667
Size: 887 2349 256 1058
Fragments arranged by size:
2349 1058 887 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 1570 1570
Size: 4550
BamHI G'GATC_C
Cuts at: 354 1575 354
Size: 1221 3329
BanI G'GyrC_C
Cuts at: 754 1566 1980 2228 2263 754
Size: 812 414 248 35 3041
Fragments arranged by size:
3041 812 414 248 35
BanII G_rGCy'C
Cuts at: 129 497 629 1166 1564 2594 129
Size: 368 132 537 398 1030 2085
Fragments arranged by size:
2085 1030 537 398 368 132
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BbsI GAAGACnn'nnnn_
Cuts at: 253 947 253
Size: 694 3856
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3504 4550 2448
Size: 1056 1046 2448
Fragments arranged by size:
2448 1056 1046
BclI T'GATC_A
Cuts at: 302 2070 302
Size: 1768 2782
BglI GCCn_nnn'nGGC
Cuts at: 1406 1406
Size: 4550
BglII A'GATC_T
Cuts at: 2065 4102 2065
Size: 2037 2513
BlpI GC'TnA_GC
Cuts at: 491 565 1662 491
Size: 74 1097 3379
Fragments arranged by size:
3379 1097 74
Bme1580I G_kGCm'C
Cuts at: 129 1290 2175 2268 3392 129
Size: 1161 885 93 1124 1287
Fragments arranged by size:
1287 1161 1124 885 93
BmrI ACTGGGnnnn_n'
Cuts at: 276 591 1762 1843 2178 4322 276
Size: 315 1171 81 335 2144 504
Fragments arranged by size:
2144 1171 504 335 315 81
BmtI G_CTAG'C
Cuts at: 691 691
Size: 4550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1094 1726 2605 3319 3617 775
Size: 319 632 879 714 298 1708
Fragments arranged by size:
1708 879 714 632 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 1006 1363 4254 1006
Size: 357 2891 1302
Fragments arranged by size:
2891 1302 357
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 2064 4101 6
Size: 2058 2037 455
Fragments arranged by size:
2058 2037 455
BsaHI Gr'CG_yC
Cuts at: 1981 2229 2931 4427 1981
Size: 248 702 1496 2104
Fragments arranged by size:
2104 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1382 1739 2260 2938 3349 3496 3770 1382
Size: 357 521 678 411 147 274 2162
Fragments arranged by size:
2162 678 521 411 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 401 431 766 796 401
Size: 30 335 30 4155
Fragments arranged by size:
4155 335 30 30
BseYI C'CCAG_C
Cuts at: 1975 2733 3398 3806 1975
Size: 758 665 408 2719
Fragments arranged by size:
2719 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1068 1363 1068
Size: 295 4255
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3368 104
Size: 1988 46 1230 1286
Fragments arranged by size:
1988 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 629 784 1166 1564 2342 2532 3392 629
Size: 155 382 398 778 190 860 1787
Fragments arranged by size:
1787 860 778 398 382 190 155
BsmBI CGTCTCn'nnnn_
Cuts at: 1052 1052
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 129 497 629 784 1166 1290 1564 2175 2268
Size: 368 132 155 382 124 274 611 93
Cuts at: 2268 2342 2532 2594 3392 129
Size: 74 190 62 798 1287
Fragments arranged by size:
1287 798 611 382 368 274 190 155
132 124 93 74 62
BspHI T'CATG_A
Cuts at: 845 2982 845
Size: 2137 2413
BspMI ACCTGCnnnn'nnnn_
Cuts at: 588 1385 1600 1814 2116 2497 588
Size: 797 215 214 302 381 2641
Fragments arranged by size:
2641 797 381 302 215 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 472 1103 2462 472
Size: 631 1359 2560
Fragments arranged by size:
2560 1359 631
BsrFI r'CCGG_y
Cuts at: 2548 2729 2548
Size: 181 4369
BssSI C'ACGA_G
Cuts at: 2821 3529 2821
Size: 708 3842
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstEII G'GTnAC_C
Cuts at: 1269 1269
Size: 4550
BstYI r'GATC_y
Cuts at: 1 354 1575 2042 2065 2400 2646 2952 2964
Size: 353 1221 467 23 335 246 306 12
Cuts at: 2964 3050 4102 1
Size: 86 1052 449
Fragments arranged by size:
1221 1052 467 449 353 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 434 1290 1340 107
Size: 327 856 50 3317
Fragments arranged by size:
3317 856 327 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 1235 1430 1793 1235
Size: 195 363 3992
Fragments arranged by size:
3992 363 195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 317 352 317
Size: 35 4515
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DraIII CAC_nnn'GTG
Cuts at: 1185 1185
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3600 2256
Size: 1344 3206
EaeI y'GGCC_r
Cuts at: 110 557 2135 2309 2700 2727 4072 110
Size: 447 1578 174 391 27 1345 588
Fragments arranged by size:
1578 1345 588 447 391 174 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 182 254 386 1004 2573 2783 182
Size: 72 132 618 1569 210 1949
Fragments arranged by size:
1949 1569 618 210 132 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 918 2758 3500 3646 4360 918
Size: 1840 742 146 714 1108
Fragments arranged by size:
1840 1108 742 714 146
EcoICRI GAG'CTC
Cuts at: 627 1164 1562 627
Size: 537 398 3615
Fragments arranged by size:
3615 537 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1018 2375 2807 3160 3822 703
Size: 315 1357 432 353 662 1431
Fragments arranged by size:
1431 1357 662 432 353 315
EcoO109I rG'GnC_Cy
Cuts at: 126 1416 1689 126
Size: 1290 273 2987
Fragments arranged by size:
2987 1290 273
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 1242 1984 2232 3462 4233 1242
Size: 742 248 1230 771 1559
Fragments arranged by size:
1559 1230 771 742 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 766 798 840 872 2392 2422 2424
Size: 32 388 32 42 32 1520 30 2
Cuts at: 2424 2454 346
Size: 30 2442
Fragments arranged by size:
2442 1520 388 42 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1589 1589
Size: 4550
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3309 3493 3309
Size: 184 4366
MscI TGG'CCA
Cuts at: 112 559 2311 112
Size: 447 1752 2351
Fragments arranged by size:
2351 1752 447
MslI CAynn'nnrTG
Cuts at: 1279 2539 2666 1279
Size: 1260 127 3163
Fragments arranged by size:
3163 1260 127
MspA1I CmG'CkG
Cuts at: 351 544 1292 1661 1975 2335 3119 3364 351
Size: 193 748 369 314 360 784 245 1537
Fragments arranged by size:
1537 784 748 369 360 314 245 193
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 4550
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NcoI C'CATG_G
Cuts at: 107 434 107
Size: 327 4223
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 4550
NheI G'CTAG_C
Cuts at: 687 687
Size: 4550
NspI r_CATG'y
Cuts at: 1603 2103 3706 1603
Size: 500 1603 2447
Fragments arranged by size:
2447 1603 500
PasI CC'CwG_GG
Cuts at: 760 760
Size: 4550
PciI A'CATG_T
Cuts at: 3702 3702
Size: 4550
PflMI CCAn_nnn'nTGG
Cuts at: 969 1185 969
Size: 216 4334
PfoI T'CCnGG_A
Cuts at: 1472 1472
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 4518
PspOMI G'GGCC_C
Cuts at: 125 125
Size: 4550
PstI C_TGCA'G
Cuts at: 350 1230 1597 2282 350
Size: 880 367 685 2618
Fragments arranged by size:
2618 880 685 367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 544 1975 2335 544
Size: 1431 360 2759
Fragments arranged by size:
2759 1431 360
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 629 1166 1564 629
Size: 537 398 3615
Fragments arranged by size:
3615 537 398
SacII CC_GC'GG
Cuts at: 1293 1293
Size: 4550
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 386 2573 2783 386
Size: 2187 210 2153
Fragments arranged by size:
2187 2153 210
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 1036 4067 1036
Size: 3031 1519
SexAI A'CCwGG_T
Cuts at: 1181 1181
Size: 4550
SfcI C'TryA_G
Cuts at: 33 346 1226 1593 2278 3246 3437 33
Size: 313 880 367 685 968 191 1146
Fragments arranged by size:
1146 968 880 685 367 313 191
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4550
SmlI C'TyrA_G
Cuts at: 181 790 1109 1705 2620 3334 3596 181
Size: 609 319 596 915 714 262 1135
Fragments arranged by size:
1135 915 714 609 596 319 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4050
StuI AGG'CCT
Cuts at: 1075 1075
Size: 4550
StyI C'CwwG_G
Cuts at: 107 420 434 1684 2038 107
Size: 313 14 1250 354 2619
Fragments arranged by size:
2619 1250 354 313 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2133 2266 2725 2934 2133
Size: 133 459 209 3749
Fragments arranged by size:
3749 459 209 133
TatI w'GTAC_w
Cuts at: 371 1034 4065 371
Size: 663 3031 856
Fragments arranged by size:
3031 856 663
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 401 433 4067 4099 401
Size: 32 3634 32 852
Fragments arranged by size:
3634 852 32 32
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 523 2045 523
Size: 1522 3028
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4550
XmnI GAAnn'nnTTC
Cuts at: 395 395
Size: 4550
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BpmI Bpu10I BseRI BsiWI
BsmI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI