Restriction map for
pF1KB5383
(Circular) (Six-base) MAPSORT of: pF1KB5383.seq Check: 6686 from: 1 to: 4730
pF1KB5383 4730 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1768 1768
Size: 4730
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 463 2555 2987 3734 4002 195
Size: 268 2092 432 747 268 923
Fragments arranged by size:
2092 923 747 432 268 268
AfeI AGC'GCT
Cuts at: 1315 1315
Size: 4730
AflIII A'CryG_T
Cuts at: 557 2082 3186 4163 557
Size: 1525 1104 977 1124
Fragments arranged by size:
1525 1124 1104 977
AgeI A'CCGG_T
Cuts at: 3118 3118
Size: 4730
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1616 1648 1616
Size: 32 4698
AlwNI CAG_nnn'CTG
Cuts at: 1641 2035 3602 1641
Size: 394 1567 2769
Fragments arranged by size:
2769 1567 394
ApaLI G'TGCA_C
Cuts at: 3500 3500
Size: 4730
ApoI r'AATT_y
Cuts at: 810 960 1108 1734 4083 4339 810
Size: 150 148 626 2349 256 1201
Fragments arranged by size:
2349 1201 626 256 150 148
AseI AT'TA_AT
Cuts at: 20 1586 20
Size: 1566 3164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 112 1721 1750 112
Size: 1609 29 3092
Fragments arranged by size:
3092 1609 29
BamHI G'GATC_C
Cuts at: 1755 1755
Size: 4730
BanI G'GyrC_C
Cuts at: 123 172 421 1746 2160 2408 2443 123
Size: 49 249 1325 414 248 35 2410
Fragments arranged by size:
2410 1325 414 249 248 49 35
BanII G_rGCy'C
Cuts at: 1368 1744 2774 1368
Size: 376 1030 3324
Fragments arranged by size:
3324 1030 376
BbeI G_GCGC'C
Cuts at: 176 2164 2412 176
Size: 1988 248 2494
Fragments arranged by size:
2494 1988 248
BbsI GAAGACnn'nnnn_
Cuts at: 1106 1106
Size: 4730
BbvCI CC'TCA_GC
Cuts at: 1193 1193
Size: 4730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4696
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3389 4730 2628
Size: 761 1341 2628
Fragments arranged by size:
2628 1341 761
BclI T'GATC_A
Cuts at: 2250 2250
Size: 4730
BglII A'GATC_T
Cuts at: 717 2245 4282 717
Size: 1528 2037 1165
Fragments arranged by size:
2037 1528 1165
BlpI GC'TnA_GC
Cuts at: 1310 1842 1310
Size: 532 4198
Bme1580I G_kGCm'C
Cuts at: 2355 2448 3504 2355
Size: 93 1056 3581
Fragments arranged by size:
3581 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1942 2023 2358 4502 1942
Size: 81 335 2144 2170
Fragments arranged by size:
2170 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3097 3097
Size: 4730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1373 1373
Size: 4730
Bpu10I CC'TnA_GC
Cuts at: 1193 1193
Size: 4730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1213 1906 2785 3277 3575 383
Size: 830 693 879 492 298 1538
Fragments arranged by size:
1538 879 830 693 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4434 4434
Size: 4730
BsaAI yAC'GTr
Cuts at: 2083 2083
Size: 4730
BsaBI GATnn'nnATC
Cuts at: 6 2244 4281 6
Size: 2238 2037 455
Fragments arranged by size:
2238 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 2161 2409 3111 4607 173
Size: 1988 248 702 1496 296
Fragments arranged by size:
1988 1496 702 296 248
BsaWI w'CCGG_w
Cuts at: 1919 2440 3118 3392 3539 3950 1919
Size: 521 678 274 147 411 2699
Fragments arranged by size:
2699 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1210 1210
Size: 4730
BseYI C'CCAG_C
Cuts at: 696 1173 2155 2913 3490 3986 696
Size: 477 982 758 577 496 1440
Fragments arranged by size:
1440 982 758 577 496 477
BsiEI CG_ry'CG
Cuts at: 104 162 2272 2318 3526 104
Size: 58 2110 46 1208 1308
Fragments arranged by size:
2110 1308 1208 58 46
BsiHKAI G_wGCw'C
Cuts at: 1389 1662 1744 2522 2712 3504 1389
Size: 273 82 778 190 792 2615
Fragments arranged by size:
2615 792 778 273 190 82
BsmI GAATG_Cn'
Cuts at: 380 1691 380
Size: 1311 3419
Bsp1286I G_dGCh'C
Cuts at: 1368 1389 1662 1744 2355 2448 2522 2712 2774
Size: 21 273 82 611 93 74 190 62
Cuts at: 2774 3504 1368
Size: 730 2594
Fragments arranged by size:
2594 730 611 273 190 93 82 74
62 21
BspEI T'CCGG_A
Cuts at: 3950 3950
Size: 4730
BspHI T'CATG_A
Cuts at: 1323 3906 1323
Size: 2583 2147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1780 1994 2296 2677 1780
Size: 214 302 381 3833
Fragments arranged by size:
3833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 134 3022 3076 4560 4725 50
Size: 84 2888 54 1484 165 55
Fragments arranged by size:
2888 1484 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 440 2642 440
Size: 2202 2528
BsrFI r'CCGG_y
Cuts at: 154 1513 1553 2728 2909 3118 154
Size: 1359 40 1175 181 209 1766
Fragments arranged by size:
1766 1359 1175 209 181 40
BssSI C'ACGA_G
Cuts at: 3001 3359 3001
Size: 358 4372
BstAPI GCAn_nnn'nTGC
Cuts at: 2107 2107
Size: 4730
BstBI TT'CG_AA
Cuts at: 3091 3091
Size: 4730
BstXI CCAn_nnnn'nTGG
Cuts at: 378 1617 378
Size: 1239 3491
BstYI r'GATC_y
Cuts at: 1 203 717 1755 2222 2245 2580 2826 3838
Size: 202 514 1038 467 23 335 246 1012
Cuts at: 3838 3924 3936 4282 1
Size: 86 12 346 449
Fragments arranged by size:
1038 1012 514 467 449 346 335 246
202 86 23 12
BtgI C'CryG_G
Cuts at: 162 162
Size: 4730
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2680 2861 3024 2680
Size: 181 163 4386
Fragments arranged by size:
4386 181 163
BtsI GCAGTG_nn'
Cuts at: 683 1973 683
Size: 1290 3440
DraI TTT'AAA
Cuts at: 959 1208 1729 959
Size: 249 521 3960
Fragments arranged by size:
3960 521 249
DraIII CAC_nnn'GTG
Cuts at: 1438 1438
Size: 4730
DrdI GACnn_nn'nnGTC
Cuts at: 558 2436 3294 558
Size: 1878 858 1994
Fragments arranged by size:
1994 1878 858
EaeI y'GGCC_r
Cuts at: 159 2315 2489 2880 2907 4252 159
Size: 2156 174 391 27 1345 637
Fragments arranged by size:
2156 1345 637 391 174 27
EagI C'GGCC_G
Cuts at: 159 2315 159
Size: 2156 2574
EarI CTCTTCn'nnn_
Cuts at: 935 1354 2753 2963 935
Size: 419 1399 210 2702
Fragments arranged by size:
2702 1399 419 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 213 2938 3248 3394 4540 213
Size: 2725 310 146 1146 403
Fragments arranged by size:
2725 1146 403 310 146
EcoICRI GAG'CTC
Cuts at: 1742 1742
Size: 4730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 463 1373 2555 2987 3734 4002 195
Size: 268 910 1182 432 747 268 923
Fragments arranged by size:
1182 923 910 747 432 268 268
EcoNI CCTnn'n_nnAGG
Cuts at: 532 532
Size: 4730
EcoO109I rG'GnC_Cy
Cuts at: 525 1869 525
Size: 1344 3386
EcoRI G'AATT_C
Cuts at: 1108 1734 1108
Size: 626 4104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4730
FspI TGC'GCA
Cuts at: 2511 2511
Size: 4730
HaeII r_GCGC'y
Cuts at: 176 1317 2164 2412 3434 4413 176
Size: 1141 847 248 1022 979 493
Fragments arranged by size:
1141 1022 979 847 493 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 980 1012 2572 2602 2604 2634 980
Size: 32 1560 30 2 30 3076
Fragments arranged by size:
3076 1560 32 30 30 2
HincII GTy'rAC
Cuts at: 1769 1769
Size: 4730
HindIII A'AGCT_T
Cuts at: 472 745 472
Size: 273 4457
KasI G'GCGC_C
Cuts at: 172 2160 2408 172
Size: 1988 248 2494
Fragments arranged by size:
2494 1988 248
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MfeI C'AATT_G
Cuts at: 758 1124 1268 758
Size: 366 144 4220
Fragments arranged by size:
4220 366 144
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 520 1634 3401 3585 520
Size: 1114 1767 184 1665
Fragments arranged by size:
1767 1665 1114 184
MscI TGG'CCA
Cuts at: 2491 2491
Size: 4730
MslI CAynn'nnrTG
Cuts at: 2719 2846 2719
Size: 127 4603
MspA1I CmG'CkG
Cuts at: 164 1841 2155 2515 3528 3773 164
Size: 1677 314 360 1013 245 1121
Fragments arranged by size:
1677 1121 1013 360 314 245
NaeI GCC'GGC
Cuts at: 156 1515 2911 156
Size: 1359 1396 1975
Fragments arranged by size:
1975 1396 1359
NarI GG'CG_CC
Cuts at: 173 2161 2409 173
Size: 1988 248 2494
Fragments arranged by size:
2494 1988 248
NgoMIV G'CCGG_C
Cuts at: 154 1513 2909 154
Size: 1359 1396 1975
Fragments arranged by size:
1975 1396 1359
NotI GC'GGCC_GC
Cuts at: 159 159
Size: 4730
NspI r_CATG'y
Cuts at: 561 1173 1561 1783 2283 3190 561
Size: 612 388 222 500 907 2101
Fragments arranged by size:
2101 907 612 500 388 222
PasI CC'CwG_GG
Cuts at: 1563 1563
Size: 4730
PciI A'CATG_T
Cuts at: 557 3186 557
Size: 2629 2101
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4698
PspXI vC'TCGA_Gb
Cuts at: 1721 1721
Size: 4730
PstI C_TGCA'G
Cuts at: 1581 1777 2462 1581
Size: 196 685 3849
Fragments arranged by size:
3849 685 196
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 2155 2515 2155
Size: 360 4370
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 1744 1744
Size: 4730
SacII CC_GC'GG
Cuts at: 165 165
Size: 4730
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 2753 2963 2753
Size: 210 4520
SbfI CC_TGCA'GG
Cuts at: 1777 1777
Size: 4730
ScaI AGT'ACT
Cuts at: 4247 4247
Size: 4730
SfcI C'TryA_G
Cuts at: 33 1532 1577 1773 2458 3451 3642 33
Size: 1499 45 196 685 993 191 1121
Fragments arranged by size:
1499 1121 993 685 196 191 45
SfoI GGC'GCC
Cuts at: 174 2162 2410 174
Size: 1988 248 2494
Fragments arranged by size:
2494 1988 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SmaI CCC'GGG
Cuts at: 1752 1752
Size: 4730
SmlI C'TyrA_G
Cuts at: 398 1228 1721 1885 2800 3292 3554 398
Size: 830 493 164 915 492 262 1574
Fragments arranged by size:
1574 915 830 493 492 262 164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 1561 1783 2283 1561
Size: 222 500 4008
Fragments arranged by size:
4008 500 222
SspI AAT'ATT
Cuts at: 1423 1423
Size: 4730
StyI C'CwwG_G
Cuts at: 261 1864 2218 261
Size: 1603 354 2773
Fragments arranged by size:
2773 1603 354
SwaI ATTT'AAAT
Cuts at: 1208 1208
Size: 4730
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 811 941 2313 2446 2905 3114 811
Size: 130 1372 133 459 209 2427
Fragments arranged by size:
2427 1372 459 209 133 130
TatI w'GTAC_w
Cuts at: 4245 4245
Size: 4730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 4698
Tth111I GACn'n_nGTC
Cuts at: 2527 2527
Size: 4730
XbaI T'CTAG_A
Cuts at: 1761 1761
Size: 4730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 882 2225 378
Size: 504 1343 2883
Fragments arranged by size:
2883 1343 504
XhoI C'TCGA_G
Cuts at: 1721 1721
Size: 4730
XmaI C'CCGG_G
Cuts at: 1750 1750
Size: 4730
XmnI GAAnn'nnTTC
Cuts at: 1682 1682
Size: 4730
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI
PciI PmeI PpiI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI NcoI NdeI NheI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI ZraI