Restriction map for
pF1KB5377
(Circular) (Six-base) MAPSORT of: pF1KB5377.seq Check: 553 from: 1 to: 4133
pF1KB5377 4133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1171 1171
Size: 4133
Acc65I G'GTAC_C
Cuts at: 367 1149 367
Size: 782 3351
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 1485 2589 3566 1485
Size: 1104 977 2052
Fragments arranged by size:
2052 1104 977
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AleI CACnn'nnGTG
Cuts at: 535 535
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 192 641 1438 3005 192
Size: 449 797 1567 1320
Fragments arranged by size:
1567 1320 797 449
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 1030 1153 1030
Size: 123 4010
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 226 358 367 1149 1563 1811 1846 226
Size: 132 9 782 414 248 35 2513
Fragments arranged by size:
2513 782 414 248 132 35 9
BanII G_rGCy'C
Cuts at: 224 572 1147 2177 224
Size: 348 575 1030 2180
Fragments arranged by size:
2180 1030 575 348
BbeI G_GCGC'C
Cuts at: 230 1567 1815 230
Size: 1337 248 2548
Fragments arranged by size:
2548 1337 248
BbsI GAAGACnn'nnnn_
Cuts at: 713 713
Size: 4133
BbvCI CC'TCA_GC
Cuts at: 254 254
Size: 4133
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2792 4133 2031
Size: 761 1341 2031
Fragments arranged by size:
2031 1341 761
BclI T'GATC_A
Cuts at: 1653 1653
Size: 4133
BglI GCCn_nnn'nGGC
Cuts at: 341 341
Size: 4133
BglII A'GATC_T
Cuts at: 293 629 1648 3685 293
Size: 336 1019 2037 741
Fragments arranged by size:
2037 1019 741 336
BlpI GC'TnA_GC
Cuts at: 271 1245 271
Size: 974 3159
Bme1580I G_kGCm'C
Cuts at: 1758 1851 2907 1758
Size: 93 1056 2984
Fragments arranged by size:
2984 1056 93
BmgBI CAC'GTC
Cuts at: 267 267
Size: 4133
BmrI ACTGGGnnnn_n'
Cuts at: 332 589 1345 1426 1761 3905 332
Size: 257 756 81 335 2144 560
Fragments arranged by size:
2144 756 560 335 257 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1027 2500 1027
Size: 1473 2660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1022
Size: 4133
Bpu10I CC'TnA_GC
Cuts at: 254 254
Size: 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1309 2188 2680 2978 1129
Size: 180 879 492 298 2284
Fragments arranged by size:
2284 879 492 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 389 773 3837 389
Size: 384 3064 685
Fragments arranged by size:
3064 685 384
BsaAI yAC'GTr
Cuts at: 1486 1486
Size: 4133
BsaBI GATnn'nnATC
Cuts at: 6 658 1647 3684 6
Size: 652 989 2037 455
Fragments arranged by size:
2037 989 652 455
BsaHI Gr'CG_yC
Cuts at: 227 1564 1812 2514 4010 227
Size: 1337 248 702 1496 350
Fragments arranged by size:
1496 1337 702 350 248
BsaWI w'CCGG_w
Cuts at: 1322 1843 2521 2795 2942 3353 1322
Size: 521 678 274 147 411 2102
Fragments arranged by size:
2102 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 772 802 889 919 772
Size: 30 87 30 3986
Fragments arranged by size:
3986 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 247 175
Size: 72 4061
BseYI C'CCAG_C
Cuts at: 1558 2316 2893 3389 1558
Size: 758 577 496 2302
Fragments arranged by size:
2302 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 897 897
Size: 4133
BsiEI CG_ry'CG
Cuts at: 104 303 1675 1721 2929 104
Size: 199 1372 46 1208 1308
Fragments arranged by size:
1372 1308 1208 199 46
BsiHKAI G_wGCw'C
Cuts at: 272 1147 1925 2115 2907 272
Size: 875 778 190 792 1498
Fragments arranged by size:
1498 875 792 778 190
BsmI GAATG_Cn'
Cuts at: 695 695
Size: 4133
Bsp1286I G_dGCh'C
Cuts at: 224 272 572 1147 1758 1851 1925 2115 2177
Size: 48 300 575 611 93 74 190 62
Cuts at: 2177 2907 224
Size: 730 1450
Fragments arranged by size:
1450 730 611 575 300 190 93 74
62 48
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1104 1183 1397 1699 2080 1104
Size: 79 214 302 381 3157
Fragments arranged by size:
3157 381 302 214 79
BsrBI CCG'CTC
Cuts at: 50 551 557 2425 2479 3963 4128 50
Size: 501 6 1868 54 1484 165 55
Fragments arranged by size:
1868 1484 501 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 2045 2045
Size: 4133
BsrFI r'CCGG_y
Cuts at: 2131 2312 2521 2131
Size: 181 209 3743
Fragments arranged by size:
3743 209 181
BssSI C'ACGA_G
Cuts at: 2404 2762 2404
Size: 358 3775
BstAPI GCAn_nnn'nTGC
Cuts at: 1510 1510
Size: 4133
BstBI TT'CG_AA
Cuts at: 699 2494 699
Size: 1795 2338
BstYI r'GATC_y
Cuts at: 1 293 588 629 1158 1625 1648 1983 2229
Size: 292 295 41 529 467 23 335 246
Cuts at: 2229 3241 3327 3339 3685 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 529 467 449 346 335 295 292
246 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 144 390 107
Size: 37 246 3850
Fragments arranged by size:
3850 246 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 1376 1376
Size: 4133
DraI TTT'AAA
Cuts at: 824 1132 824
Size: 308 3825
DrdI GACnn_nn'nnGTC
Cuts at: 613 1839 2697 613
Size: 1226 858 2049
Fragments arranged by size:
2049 1226 858
EaeI y'GGCC_r
Cuts at: 546 1718 1892 2283 2310 3655 546
Size: 1172 174 391 27 1345 1024
Fragments arranged by size:
1345 1172 1024 391 174 27
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 620 2156 2366 620
Size: 1536 210 2387
Fragments arranged by size:
2387 1536 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1145 1145
Size: 4133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1958 2390 3137 3405 1022
Size: 936 432 747 268 1750
Fragments arranged by size:
1750 936 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 895 1004 895
Size: 109 4024
EcoO109I rG'GnC_Cy
Cuts at: 1044 1272 1044
Size: 228 3905
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
EcoRV GAT'ATC
Cuts at: 810 810
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 4133
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 193 230 1567 1815 2837 3816 193
Size: 37 1337 248 1022 979 510
Fragments arranged by size:
1337 1022 979 510 248 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 772 804 1975 2005 2007 2037 484
Size: 32 256 32 1171 30 2 30 2580
Fragments arranged by size:
2580 1171 256 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
KasI G'GCGC_C
Cuts at: 226 1563 1811 226
Size: 1337 248 2548
Fragments arranged by size:
2548 1337 248
KpnI G_GTAC'C
Cuts at: 371 1153 371
Size: 782 3351
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 815 2804 2988 134
Size: 681 1989 184 1279
Fragments arranged by size:
1989 1279 681 184
MscI TGG'CCA
Cuts at: 1894 1894
Size: 4133
MslI CAynn'nnrTG
Cuts at: 535 727 2122 2249 535
Size: 192 1395 127 2419
Fragments arranged by size:
2419 1395 192 127
MspA1I CmG'CkG
Cuts at: 146 462 638 805 1244 1558 1918 2931 3176
Size: 316 176 167 439 314 360 1013 245
Cuts at: 3176 146
Size: 1103
Fragments arranged by size:
1103 1013 439 360 316 314 245 176
167
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 227 1564 1812 227
Size: 1337 248 2548
Fragments arranged by size:
2548 1337 248
NcoI C'CATG_G
Cuts at: 107 390 107
Size: 283 3850
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 987 1186 1686 2593 987
Size: 199 500 907 2527
Fragments arranged by size:
2527 907 500 199
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 132 502 132
Size: 370 3763
PfoI T'CCnGG_A
Cuts at: 893 999 893
Size: 106 4027
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 223 255 3272 3304 223
Size: 32 3017 32 1052
Fragments arranged by size:
3017 1052 32 32
PpuMI rG'GwC_Cy
Cuts at: 1044 1044
Size: 4133
PstI C_TGCA'G
Cuts at: 1180 1865 1180
Size: 685 3448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 805 1558 1918 805
Size: 753 360 3020
Fragments arranged by size:
3020 753 360
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 1147 1147
Size: 4133
SacII CC_GC'GG
Cuts at: 147 147
Size: 4133
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 1180 1180
Size: 4133
ScaI AGT'ACT
Cuts at: 3650 3650
Size: 4133
SfcI C'TryA_G
Cuts at: 33 633 1176 1861 2854 3045 33
Size: 600 543 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 543 191
SfoI GGC'GCC
Cuts at: 228 1565 1813 228
Size: 1337 248 2548
Fragments arranged by size:
2548 1337 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 1108 1288 2203 2695 2957 1108
Size: 180 915 492 262 2284
Fragments arranged by size:
2284 915 492 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 987 1186 1686 987
Size: 199 500 3434
Fragments arranged by size:
3434 500 199
SspI AAT'ATT
Cuts at: 716 716
Size: 4133
StuI AGG'CCT
Cuts at: 259 320 978 1010 259
Size: 61 658 32 3382
Fragments arranged by size:
3382 658 61 32
StyI C'CwwG_G
Cuts at: 107 390 770 1267 1621 107
Size: 283 380 497 354 2619
Fragments arranged by size:
2619 497 380 354 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 511 616 1716 1849 2308 2517 511
Size: 105 1100 133 459 209 2127
Fragments arranged by size:
2127 1100 459 209 133 105
TatI w'GTAC_w
Cuts at: 3648 3648
Size: 4133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 889 921 3650 3682 889
Size: 32 2729 32 1340
Fragments arranged by size:
2729 1340 32 32
Tth111I GACn'n_nGTC
Cuts at: 1930 1930
Size: 4133
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1628 1628
Size: 4133
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
XmnI GAAnn'nnTTC
Cuts at: 1082 1082
Size: 4133
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI