Restriction map for
pF1KB5370
(Circular) (Six-base) MAPSORT of: pF1KB5370.seq Check: 7398 from: 1 to: 4028
pF1KB5370 4028 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 911 911
Size: 4028
AccI GT'mk_AC
Cuts at: 455 1066 455
Size: 611 3417
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 541 734 1853 2285 3032 3300 481
Size: 60 193 1119 432 747 268 1209
Fragments arranged by size:
1209 1119 747 432 268 193 60
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 510 1333 2900 510
Size: 823 1567 1638
Fragments arranged by size:
1638 1567 823
ApaLI G'TGCA_C
Cuts at: 469 2798 469
Size: 2329 1699
ApoI r'AATT_y
Cuts at: 478 1032 3381 3637 478
Size: 554 2349 256 869
Fragments arranged by size:
2349 869 554 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 626 1048 626
Size: 422 3606
BamHI G'GATC_C
Cuts at: 113 1053 113
Size: 940 3088
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 127 187 1042 2072 127
Size: 60 855 1030 2083
Fragments arranged by size:
2083 1030 855 60
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 473 1653 1746 2802 473
Size: 1180 93 1056 1699
Fragments arranged by size:
1699 1180 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 652 720 1000 1240 1321 1656 3800 652
Size: 68 280 240 81 335 2144 880
Fragments arranged by size:
2144 880 335 280 240 81 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 896
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 2575 2873 1204
Size: 879 492 298 2359
Fragments arranged by size:
2359 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 422 1542 3579 6
Size: 416 1120 2037 455
Fragments arranged by size:
2037 1120 455 416
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1453 2211 2788 3284 1453
Size: 758 577 496 2197
Fragments arranged by size:
2197 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 979
Size: 4028
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 473 1042 1820 2010 2802 473
Size: 569 778 190 792 1699
Fragments arranged by size:
1699 792 778 569 190
Bsp1286I G_dGCh'C
Cuts at: 127 187 473 1042 1653 1746 1820 2010 2072
Size: 60 286 569 611 93 74 190 62
Cuts at: 2072 2802 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 569 286 190 93 74
62 60
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 911 1078 1292 1594 1975 257
Size: 654 167 214 302 381 2310
Fragments arranged by size:
2310 654 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 910 2320 2374 3858 4023 50
Size: 860 1410 54 1484 165 55
Fragments arranged by size:
1484 1410 860 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 209 655 2026 2207 2416 209
Size: 446 1371 181 209 1821
Fragments arranged by size:
1821 1371 446 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 854 854
Size: 4028
BstYI r'GATC_y
Cuts at: 1 113 438 717 1053 1520 1543 1878 2124
Size: 112 325 279 336 467 23 335 246
Cuts at: 2124 3136 3222 3234 3580 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 336 335 325 279
246 112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 215 485 215
Size: 270 3758
BtgI C'CryG_G
Cuts at: 107 107
Size: 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 354 389 354
Size: 35 3993
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 1613 1787 2178 2205 3550 1613
Size: 174 391 27 1345 2091
Fragments arranged by size:
2091 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 541 734 896 1853 2285 3032 3300 481
Size: 60 193 162 957 432 747 268 1209
Fragments arranged by size:
1209 957 747 432 268 193 162 60
EcoNI CCTnn'n_nnAGG
Cuts at: 488 488
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 203 1167 203
Size: 964 3064
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
EcoRV GAT'ATC
Cuts at: 765 765
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1870 1900 1902 1932 1870
Size: 30 2 30 3966
Fragments arranged by size:
3966 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 745 2699 2883 745
Size: 1954 184 1890
Fragments arranged by size:
1954 1890 184
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 676 2017 2144 676
Size: 1341 127 2560
Fragments arranged by size:
2560 1341 127
MspA1I CmG'CkG
Cuts at: 687 963 1139 1453 1813 2826 3071 687
Size: 276 176 314 360 1013 245 1644
Fragments arranged by size:
1644 1013 360 314 276 245 176
NaeI GCC'GGC
Cuts at: 211 657 2209 211
Size: 446 1552 2030
Fragments arranged by size:
2030 1552 446
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 107
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 209 655 2207 209
Size: 446 1552 2030
Fragments arranged by size:
2030 1552 446
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PasI CC'CwG_GG
Cuts at: 144 144
Size: 4028
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 330 330
Size: 4028
PfoI T'CCnGG_A
Cuts at: 227 893 227
Size: 666 3362
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PpuMI rG'GwC_Cy
Cuts at: 203 203
Size: 4028
PstI C_TGCA'G
Cuts at: 797 1075 1760 797
Size: 278 685 3065
Fragments arranged by size:
3065 685 278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 687 963 1453 1813 687
Size: 276 490 360 2902
Fragments arranged by size:
2902 490 360 276
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 682 793 861 1071 1756 2749 2940 33
Size: 649 111 68 210 685 993 191 1121
Fragments arranged by size:
1121 993 685 649 210 191 111 68
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 1183 2098 2590 2852 1183
Size: 915 492 262 2359
Fragments arranged by size:
2359 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 107 1162 1516 107
Size: 1055 354 2619
Fragments arranged by size:
2619 1055 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 1744 2203 2412 1611
Size: 133 459 209 3227
Fragments arranged by size:
3227 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI