Restriction map for
pF1KB5369
(Circular) (Six-base) MAPSORT of: pF1KB5369.seq Check: 9138 from: 1 to: 4685
pF1KB5369 4685 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 151 1523 151
Size: 1372 3313
AccI GT'mk_AC
Cuts at: 1723 1723
Size: 4685
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 2510 2942 3295 3957 220
Size: 2290 432 353 662 948
Fragments arranged by size:
2290 948 662 432 353
AflIII A'CryG_T
Cuts at: 782 2037 3837 4118 782
Size: 1255 1800 281 1349
Fragments arranged by size:
1800 1349 1255 281
AleI CACnn'nnGTG
Cuts at: 253 610 253
Size: 357 4328
AlwNI CAG_nnn'CTG
Cuts at: 508 1065 1152 1990 3428 508
Size: 557 87 838 1438 1765
Fragments arranged by size:
1765 1438 838 557 87
ApaI G_GGCC'C
Cuts at: 1504 1504
Size: 4685
ApaLI G'TGCA_C
Cuts at: 3523 3523
Size: 4685
ApoI r'AATT_y
Cuts at: 1689 4038 4294 1689
Size: 2349 256 2080
Fragments arranged by size:
2349 2080 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 1705 1705
Size: 4685
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 139 442 484 1124 1701 2115 2363 2398 139
Size: 303 42 640 577 414 248 35 2426
Fragments arranged by size:
2426 640 577 414 303 248 42 35
BanII G_rGCy'C
Cuts at: 1504 1699 2729 1504
Size: 195 1030 3460
Fragments arranged by size:
3460 1030 195
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbsI GAAGACnn'nnnn_
Cuts at: 1220 1245 1482 1220
Size: 25 237 4423
Fragments arranged by size:
4423 237 25
BciVI GTATCCnnnnn_n'
Cuts at: 1261 2583 3639 4685 1261
Size: 1322 1056 1046 1261
Fragments arranged by size:
1322 1261 1056 1046
BclI T'GATC_A
Cuts at: 758 2205 758
Size: 1447 3238
BfrBI ATG'CAT
Cuts at: 735 735
Size: 4685
BglI GCCn_nnn'nGGC
Cuts at: 649 1147 1612 649
Size: 498 465 3722
Fragments arranged by size:
3722 498 465
BglII A'GATC_T
Cuts at: 2200 4237 2200
Size: 2037 2648
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 487 841 1127 1451 1504 2310 2403 3527 487
Size: 354 286 324 53 806 93 1124 1645
Fragments arranged by size:
1645 1124 806 354 324 286 93 53
BmgBI CAC'GTC
Cuts at: 756 1251 1482 756
Size: 495 231 3959
Fragments arranged by size:
3959 495 231
BmrI ACTGGGnnnn_n'
Cuts at: 573 1897 1978 2313 4457 573
Size: 1324 81 335 2144 801
Fragments arranged by size:
2144 1324 801 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 567 1416 3052 567
Size: 849 1636 2200
Fragments arranged by size:
2200 1636 849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 388 250
Size: 138 4547
Bpu10I CC'TnA_GC
Cuts at: 1666 1666
Size: 4685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 717 1861 2740 3454 3752 310
Size: 407 1144 879 714 298 1243
Fragments arranged by size:
1243 1144 879 714 407 298
BsaI GGTCTCn'nnnn_
Cuts at: 191 352 976 1456 4389 191
Size: 161 624 480 2933 487
Fragments arranged by size:
2933 624 487 480 161
BsaAI yAC'GTr
Cuts at: 1554 2038 1554
Size: 484 4201
BsaBI GATnn'nnATC
Cuts at: 6 2199 4236 6
Size: 2193 2037 455
Fragments arranged by size:
2193 2037 455
BsaHI Gr'CG_yC
Cuts at: 1526 2116 2364 3066 4562 1526
Size: 590 248 702 1496 1649
Fragments arranged by size:
1649 1496 702 590 248
BsaWI w'CCGG_w
Cuts at: 1874 2395 3073 3484 3631 3905 1874
Size: 521 678 411 147 274 2654
Fragments arranged by size:
2654 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 886 916 886
Size: 30 4655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 1197 370
Size: 827 3858
BseYI C'CCAG_C
Cuts at: 577 628 2110 2868 3533 3941 577
Size: 51 1482 758 665 408 1321
Fragments arranged by size:
1482 1321 758 665 408 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1504 544
Size: 960 3725
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3503 104
Size: 2123 46 1230 1286
Fragments arranged by size:
2123 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1602 1699 2477 2667 3527 1602
Size: 97 778 190 860 2760
Fragments arranged by size:
2760 860 778 190 97
Bsp1286I G_dGCh'C
Cuts at: 487 841 1127 1451 1504 1602 1699 2310 2403
Size: 354 286 324 53 98 97 611 93
Cuts at: 2403 2477 2667 2729 3527 487
Size: 74 190 62 798 1645
Fragments arranged by size:
1645 798 611 354 324 286 190 98
97 93 74 62 53
BspHI T'CATG_A
Cuts at: 3117 3117
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 151 1523 1735 1949 2251 2632 151
Size: 1372 212 214 302 381 2204
Fragments arranged by size:
2204 1372 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 2977 3031 4515 4680 50
Size: 2927 54 1484 165 55
Fragments arranged by size:
2927 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1362 2597 1362
Size: 1235 3450
BsrFI r'CCGG_y
Cuts at: 439 2683 2864 439
Size: 2244 181 2260
Fragments arranged by size:
2260 2244 181
BssSI C'ACGA_G
Cuts at: 606 840 1459 1531 2956 3664 606
Size: 234 619 72 1425 708 1627
Fragments arranged by size:
1627 1425 708 619 234 72
BstAPI GCAn_nnn'nTGC
Cuts at: 1152 1518 2062 1152
Size: 366 544 3775
Fragments arranged by size:
3775 544 366
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstEII G'GTnAC_C
Cuts at: 546 546
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 349 349
Size: 4685
BstYI r'GATC_y
Cuts at: 1 1061 1710 2177 2200 2535 2781 3087 3099
Size: 1060 649 467 23 335 246 306 12
Cuts at: 3099 3185 4237 1
Size: 86 1052 449
Fragments arranged by size:
1060 1052 649 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 689 689
Size: 4685
BtgI C'CryG_G
Cuts at: 488 1119 1226 1278 1403 1467 1505 488
Size: 631 107 52 125 64 38 3668
Fragments arranged by size:
3668 631 125 107 64 52 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 702 2635 2816 2979 702
Size: 1933 181 163 2408
Fragments arranged by size:
2408 1933 181 163
BtsI GCAGTG_nn'
Cuts at: 1232 1337 1928 1232
Size: 105 591 3989
Fragments arranged by size:
3989 591 105
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DraIII CAC_nnn'GTG
Cuts at: 954 954
Size: 4685
DrdI GACnn_nn'nnGTC
Cuts at: 1026 1486 2391 3735 1026
Size: 460 905 1344 1976
Fragments arranged by size:
1976 1344 905 460
EaeI y'GGCC_r
Cuts at: 131 771 1052 1484 1590 2270 2444 2835 2862
Size: 640 281 432 106 680 174 391 27
Cuts at: 2862 4207 131
Size: 1345 609
Fragments arranged by size:
1345 680 640 609 432 391 281 174
106 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 871 979 2708 2918 871
Size: 108 1729 210 2638
Fragments arranged by size:
2638 1729 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3635 3781 4495 2893
Size: 742 146 714 3083
Fragments arranged by size:
3083 742 714 146
EcoICRI GAG'CTC
Cuts at: 1697 1697
Size: 4685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 250 388 2510 2942 3295 3957 220
Size: 30 138 2122 432 353 662 948
Fragments arranged by size:
2122 948 662 432 353 138 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1156 1156
Size: 4685
EcoO109I rG'GnC_Cy
Cuts at: 426 1149 1500 1501 1633 1824 426
Size: 723 351 1 132 191 3287
Fragments arranged by size:
3287 723 351 191 132 1
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 402 1350 2704 402
Size: 948 1354 2383
Fragments arranged by size:
2383 1354 948
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 440 635 2119 2367 3597 4368 440
Size: 195 1484 248 1230 771 757
Fragments arranged by size:
1484 1230 771 757 248 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 652 684 1382 1414 2527 2557 2559 2589 652
Size: 32 698 32 1113 30 2 30 2748
Fragments arranged by size:
2748 1113 698 32 32 30 30 2
HincII GTy'rAC
Cuts at: 867 1724 867
Size: 857 3828
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3444 3628 3444
Size: 184 4501
MscI TGG'CCA
Cuts at: 133 1054 2446 133
Size: 921 1392 2372
Fragments arranged by size:
2372 1392 921
MslI CAynn'nnrTG
Cuts at: 253 610 754 2674 2801 253
Size: 357 144 1920 127 2137
Fragments arranged by size:
2137 1920 357 144 127
MspA1I CmG'CkG
Cuts at: 1796 2110 2470 3254 3499 1796
Size: 314 360 784 245 2982
Fragments arranged by size:
2982 784 360 314 245
NaeI GCC'GGC
Cuts at: 441 2866 441
Size: 2425 2260
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NcoI C'CATG_G
Cuts at: 1403 1505 1403
Size: 102 4583
NgoMIV G'CCGG_C
Cuts at: 439 2864 439
Size: 2425 2260
NsiI A_TGCA'T
Cuts at: 737 737
Size: 4685
NspI r_CATG'y
Cuts at: 786 993 1738 2238 3841 786
Size: 207 745 500 1603 1630
Fragments arranged by size:
1630 1603 745 500 207
PasI CC'CwG_GG
Cuts at: 349 534 349
Size: 185 4500
PciI A'CATG_T
Cuts at: 782 3837 782
Size: 3055 1630
PflMI CCAn_nnn'nTGG
Cuts at: 191 954 1557 191
Size: 763 603 3319
Fragments arranged by size:
3319 763 603
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PmlI CAC'GTG
Cuts at: 1554 1554
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 4653
PpuMI rG'GwC_Cy
Cuts at: 426 426
Size: 4685
PshAI GACnn'nnGTC
Cuts at: 1228 1228
Size: 4685
PspOMI G'GGCC_C
Cuts at: 1500 1500
Size: 4685
PstI C_TGCA'G
Cuts at: 1632 1732 2417 1632
Size: 100 685 3900
Fragments arranged by size:
3900 685 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 2110 2470 2110
Size: 360 4325
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 1699 1699
Size: 4685
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 871 2708 2918 871
Size: 1837 210 2638
Fragments arranged by size:
2638 1837 210
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 128 190 4202 128
Size: 62 4012 611
Fragments arranged by size:
4012 611 62
SfcI C'TryA_G
Cuts at: 33 1628 1728 2413 3381 3572 33
Size: 1595 100 685 968 191 1146
Fragments arranged by size:
1595 1146 968 685 191 100
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4685
SmlI C'TyrA_G
Cuts at: 325 696 1840 2755 3469 3731 325
Size: 371 1144 915 714 262 1279
Fragments arranged by size:
1279 1144 915 714 371 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
StuI AGG'CCT
Cuts at: 694 694
Size: 4685
StyI C'CwwG_G
Cuts at: 569 1403 1496 1505 1819 2173 569
Size: 834 93 9 314 354 3081
Fragments arranged by size:
3081 834 354 314 93 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 547 2268 2401 2860 3069 283
Size: 264 1721 133 459 209 1899
Fragments arranged by size:
1899 1721 459 264 209 133
TatI w'GTAC_w
Cuts at: 126 188 4200 126
Size: 62 4012 611
Fragments arranged by size:
4012 611 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4202 4234 4202
Size: 32 4653
Tth111I GACn'n_nGTC
Cuts at: 2482 2482
Size: 4685
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 346 369 1503 2180 346
Size: 23 1134 677 2851
Fragments arranged by size:
2851 1134 677 23
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4685
XmnI GAAnn'nnTTC
Cuts at: 880 1333 880
Size: 453 4232
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BmtI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PfoI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI