Restriction map for
pF1KB5366
(Circular) (Six-base) MAPSORT of: pF1KB5366.seq Check: 7667 from: 1 to: 3746
pF1KB5366 3746 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 302 762 302
Size: 460 3286
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 478 551 1571 2003 2750 3018 318
Size: 160 73 1020 432 747 268 1046
Fragments arranged by size:
1046 1020 747 432 268 160 73
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 564 2134 564
Size: 1570 2176
AleI CACnn'nnGTG
Cuts at: 313 313
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 456 1051 2618 456
Size: 595 1567 1584
Fragments arranged by size:
1584 1567 595
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 630 750 3099 3355 630
Size: 120 2349 256 1021
Fragments arranged by size:
2349 1021 256 120
AscI GG'CGCG_CC
Cuts at: 235 235
Size: 3746
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 605 766 605
Size: 161 3585
BamHI G'GATC_C
Cuts at: 402 771 402
Size: 369 3377
BanI G'GyrC_C
Cuts at: 111 302 762 1176 1424 1459 111
Size: 191 460 414 248 35 2398
Fragments arranged by size:
2398 460 414 248 191 35
BanII G_rGCy'C
Cuts at: 350 760 1790 350
Size: 410 1030 2306
Fragments arranged by size:
2306 1030 410
BbeI G_GCGC'C
Cuts at: 115 1180 1428 115
Size: 1065 248 2433
Fragments arranged by size:
2433 1065 248
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglI GCCn_nnn'nGGC
Cuts at: 125 125
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 1371 1464 2520 1371
Size: 93 1056 2597
Fragments arranged by size:
2597 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 2113 486
Size: 1627 2119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 3746
Bpu10I CC'TnA_GC
Cuts at: 349 349
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 179 1099 179
Size: 920 2826
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 112 1177 1425 2127 3623 112
Size: 1065 248 702 1496 235
Fragments arranged by size:
1496 1065 702 248 235
BsaWI w'CCGG_w
Cuts at: 559 564 578 935 1456 2134 2408 2555 2966
Size: 5 14 357 521 678 274 147 411
Cuts at: 2966 559
Size: 1339
Fragments arranged by size:
1339 678 521 411 357 274 147 14
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 423 358
Size: 65 3681
BseYI C'CCAG_C
Cuts at: 115 551 1171 1929 2506 3002 115
Size: 436 620 758 577 496 859
Fragments arranged by size:
859 758 620 577 496 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 469
Size: 3746
BsiEI CG_ry'CG
Cuts at: 104 327 1288 1334 2542 104
Size: 223 961 46 1208 1308
Fragments arranged by size:
1308 1208 961 223 46
BsiHKAI G_wGCw'C
Cuts at: 350 760 1538 1728 2520 350
Size: 410 778 190 792 1576
Fragments arranged by size:
1576 792 778 410 190
BsmI GAATG_Cn'
Cuts at: 362 362
Size: 3746
Bsp1286I G_dGCh'C
Cuts at: 350 760 1371 1464 1538 1728 1790 2520 350
Size: 410 611 93 74 190 62 730 1576
Fragments arranged by size:
1576 730 611 410 190 93 74 62
BspEI T'CCGG_A
Cuts at: 578 2966 578
Size: 2388 1358
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 470 2038 2092 3576 3741 50
Size: 420 1568 54 1484 165 55
Fragments arranged by size:
1568 1484 420 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 192 322 358 564 1744 1925 2134 192
Size: 130 36 206 1180 181 209 1804
Fragments arranged by size:
1804 1180 209 206 181 130 36
BssHII G'CGCG_C
Cuts at: 235 318 235
Size: 83 3663
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 203 402 771 1238 1261 1596 1842 2854
Size: 202 199 369 467 23 335 246 1012
Cuts at: 2854 2940 2952 3298 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 369 346 335 246 202
199 86 23 12
BtgI C'CryG_G
Cuts at: 107 293 327 425 107
Size: 186 34 98 3428
Fragments arranged by size:
3428 186 98 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 1696 1877 2040 228
Size: 1468 181 163 1934
Fragments arranged by size:
1934 1468 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 173 324 356 545 1331 1505 1896 1923 3268
Size: 151 32 189 786 174 391 27 1345
Cuts at: 3268 173
Size: 651
Fragments arranged by size:
1345 786 651 391 189 174 151 32
27
EagI C'GGCC_G
Cuts at: 324 1331 324
Size: 1007 2739
EarI CTCTTCn'nnn_
Cuts at: 490 617 685 1769 1979 490
Size: 127 68 1084 210 2257
Fragments arranged by size:
2257 1084 210 127 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 429 1954 2264 2410 3556 429
Size: 1525 310 146 1146 619
Fragments arranged by size:
1525 1146 619 310 146
EcoICRI GAG'CTC
Cuts at: 348 758 348
Size: 410 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 361 478 551 1571 2003 2750 3018 318
Size: 43 117 73 1020 432 747 268 1046
Fragments arranged by size:
1046 1020 747 432 268 117 73 43
EcoO109I rG'GnC_Cy
Cuts at: 265 885 265
Size: 620 3126
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 162 1765 162
Size: 1603 2143
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 115 1180 1428 2450 3429 115
Size: 1065 248 1022 979 432
Fragments arranged by size:
1065 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 111 1176 1424 111
Size: 1065 248 2433
Fragments arranged by size:
2433 1065 248
KpnI G_GTAC'C
Cuts at: 306 766 306
Size: 460 3286
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 175 547 1507 175
Size: 372 960 2414
Fragments arranged by size:
2414 960 372
MslI CAynn'nnrTG
Cuts at: 313 397 1735 1862 313
Size: 84 1338 127 2197
Fragments arranged by size:
2197 1338 127 84
MspA1I CmG'CkG
Cuts at: 165 329 453 555 857 1171 1531 2544 2789
Size: 164 124 102 302 314 360 1013 245
Cuts at: 2789 165
Size: 1122
Fragments arranged by size:
1122 1013 360 314 302 245 164 124
102
NaeI GCC'GGC
Cuts at: 194 324 360 1927 194
Size: 130 36 1567 2013
Fragments arranged by size:
2013 1567 130 36
NarI GG'CG_CC
Cuts at: 112 1177 1425 112
Size: 1065 248 2433
Fragments arranged by size:
2433 1065 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 192 322 358 1925 192
Size: 130 36 1567 2013
Fragments arranged by size:
2013 1567 130 36
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PmlI CAC'GTG
Cuts at: 179 179
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PpuMI rG'GwC_Cy
Cuts at: 265 265
Size: 3746
PspXI vC'TCGA_Gb
Cuts at: 605 605
Size: 3746
PstI C_TGCA'G
Cuts at: 440 793 1478 440
Size: 353 685 2708
Fragments arranged by size:
2708 685 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 555 1171 1531 555
Size: 616 360 2770
Fragments arranged by size:
2770 616 360
RsrII CG'GwC_CG
Cuts at: 562 1941 562
Size: 1379 2367
SacI G_AGCT'C
Cuts at: 350 760 350
Size: 410 3336
SacII CC_GC'GG
Cuts at: 330 330
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SanDI GG'GwC_CC
Cuts at: 265 265
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 685 1769 1979 685
Size: 1084 210 2452
Fragments arranged by size:
2452 1084 210
SbfI CC_TGCA'GG
Cuts at: 440 793 440
Size: 353 3393
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 436 789 1474 2467 2658 33
Size: 403 353 685 993 191 1121
Fragments arranged by size:
1121 993 685 403 353 191
SfoI GGC'GCC
Cuts at: 113 1178 1426 113
Size: 1065 248 2433
Fragments arranged by size:
2433 1065 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 605 901 1816 2308 2570 605
Size: 296 915 492 262 1781
Fragments arranged by size:
1781 915 492 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 230 704 880 1234 107
Size: 123 474 176 354 2619
Fragments arranged by size:
2619 474 354 176 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 1329 1462 1921 2130 565
Size: 764 133 459 209 2181
Fragments arranged by size:
2181 764 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1241
Size: 3746
XhoI C'TCGA_G
Cuts at: 605 605
Size: 3746
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 631 631
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PshAI PsiI PspOMI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI