Restriction map for
pF1KB5361
(Circular) (Six-base) MAPSORT of: pF1KB5361.seq Check: 5677 from: 1 to: 4400
pF1KB5361 4400 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1438 1438
Size: 4400
Acc65I G'GTAC_C
Cuts at: 140 1416 140
Size: 1276 3124
AclI AA'CG_TT
Cuts at: 4240 4240
Size: 4400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2657 3404 3672 2225
Size: 432 747 268 2953
Fragments arranged by size:
2953 747 432 268
AflIII A'CryG_T
Cuts at: 1752 2856 3833 1752
Size: 1104 977 2319
Fragments arranged by size:
2319 1104 977
AgeI A'CCGG_T
Cuts at: 2788 2788
Size: 4400
AhdI GACnn_n'nnGTC
Cuts at: 1376 1376
Size: 4400
AlwNI CAG_nnn'CTG
Cuts at: 655 1251 1705 3272 655
Size: 596 454 1567 1783
Fragments arranged by size:
1783 1567 596 454
ApaLI G'TGCA_C
Cuts at: 286 3170 286
Size: 2884 1516
ApoI r'AATT_y
Cuts at: 1404 3753 4009 1404
Size: 2349 256 1795
Fragments arranged by size:
2349 1795 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4400
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
AvaI C'yCGr_G
Cuts at: 1009 1420 1009
Size: 411 3989
BamHI G'GATC_C
Cuts at: 925 1425 925
Size: 500 3900
BanI G'GyrC_C
Cuts at: 140 1416 1830 2078 2113 140
Size: 1276 414 248 35 2427
Fragments arranged by size:
2427 1276 414 248 35
BanII G_rGCy'C
Cuts at: 1414 2444 1414
Size: 1030 3370
BbeI G_GCGC'C
Cuts at: 1834 2082 1834
Size: 248 4152
BbsI GAAGACnn'nnnn_
Cuts at: 617 617
Size: 4400
BciVI GTATCCnnnnn_n'
Cuts at: 2298 3059 4400 2298
Size: 761 1341 2298
Fragments arranged by size:
2298 1341 761
BclI T'GATC_A
Cuts at: 1920 1920
Size: 4400
BglI GCCn_nnn'nGGC
Cuts at: 1105 1105
Size: 4400
BglII A'GATC_T
Cuts at: 741 1915 3952 741
Size: 1174 2037 1189
Fragments arranged by size:
2037 1189 1174
BlpI GC'TnA_GC
Cuts at: 1073 1244 1512 1073
Size: 171 268 3961
Fragments arranged by size:
3961 268 171
Bme1580I G_kGCm'C
Cuts at: 290 2025 2118 3174 290
Size: 1735 93 1056 1516
Fragments arranged by size:
1735 1516 1056 93
BmgBI CAC'GTC
Cuts at: 132 132
Size: 4400
BmrI ACTGGGnnnn_n'
Cuts at: 1612 1693 2028 4172 1612
Size: 81 335 2144 1840
Fragments arranged by size:
2144 1840 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 935 1576 2455 2947 3245 314
Size: 621 641 879 492 298 1469
Fragments arranged by size:
1469 879 641 621 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 4104 397
Size: 3707 693
BsaAI yAC'GTr
Cuts at: 1753 1753
Size: 4400
BsaBI GATnn'nnATC
Cuts at: 6 740 1914 3951 6
Size: 734 1174 2037 455
Fragments arranged by size:
2037 1174 734 455
BsaHI Gr'CG_yC
Cuts at: 934 1831 2079 2781 4277 934
Size: 897 248 702 1496 1057
Fragments arranged by size:
1496 1057 897 702 248
BsaWI w'CCGG_w
Cuts at: 852 1589 2110 2788 3062 3209 3620 852
Size: 737 521 678 274 147 411 1632
Fragments arranged by size:
1632 737 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 187
Size: 4400
BseYI C'CCAG_C
Cuts at: 429 1000 1178 1277 1288 1825 2583 3160 3656
Size: 571 178 99 11 537 758 577 496
Cuts at: 3656 429
Size: 1173
Fragments arranged by size:
1173 758 577 571 537 496 178 99
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 212
Size: 4400
BsiEI CG_ry'CG
Cuts at: 104 1942 1988 3196 104
Size: 1838 46 1208 1308
Fragments arranged by size:
1838 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 290 1414 2192 2382 3174 290
Size: 1124 778 190 792 1516
Fragments arranged by size:
1516 1124 792 778 190
BsmI GAATG_Cn'
Cuts at: 251 251
Size: 4400
Bsp1286I G_dGCh'C
Cuts at: 290 1414 2025 2118 2192 2382 2444 3174 290
Size: 1124 611 93 74 190 62 730 1516
Fragments arranged by size:
1516 1124 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 852 3620 852
Size: 2768 1632
BspHI T'CATG_A
Cuts at: 3576 3576
Size: 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 1450 1664 1966 2347 170
Size: 1280 214 302 381 2223
Fragments arranged by size:
2223 1280 381 302 214
BsrBI CCG'CTC
Cuts at: 50 973 2692 2746 4230 4395 50
Size: 923 1719 54 1484 165 55
Fragments arranged by size:
1719 1484 923 165 55 54
BsrDI GCAATG_nn'
Cuts at: 698 962 1261 2312 698
Size: 264 299 1051 2786
Fragments arranged by size:
2786 1051 299 264
BsrFI r'CCGG_y
Cuts at: 820 2398 2579 2788 820
Size: 1578 181 209 2432
Fragments arranged by size:
2432 1578 209 181
BssSI C'ACGA_G
Cuts at: 2671 3029 2671
Size: 358 4042
BstAPI GCAn_nnn'nTGC
Cuts at: 817 1777 817
Size: 960 3440
BstBI TT'CG_AA
Cuts at: 2761 2761
Size: 4400
BstXI CCAn_nnnn'nTGG
Cuts at: 1186 1186
Size: 4400
BstYI r'GATC_y
Cuts at: 1 741 925 1425 1892 1915 2250 2496 3508
Size: 740 184 500 467 23 335 246 1012
Cuts at: 3508 3594 3606 3952 1
Size: 86 12 346 449
Fragments arranged by size:
1012 740 500 467 449 346 335 246
184 86 23 12
BtgI C'CryG_G
Cuts at: 1103 1103
Size: 4400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 231 2350 2531 2694 231
Size: 2119 181 163 1937
Fragments arranged by size:
2119 1937 181 163
BtsI GCAGTG_nn'
Cuts at: 1643 1643
Size: 4400
DraI TTT'AAA
Cuts at: 1399 1399
Size: 4400
DrdI GACnn_nn'nnGTC
Cuts at: 117 2106 2964 117
Size: 1989 858 1553
Fragments arranged by size:
1989 1553 858
EaeI y'GGCC_r
Cuts at: 155 1106 1985 2159 2550 2577 3922 155
Size: 951 879 174 391 27 1345 633
Fragments arranged by size:
1345 951 879 633 391 174 27
EagI C'GGCC_G
Cuts at: 1985 1985
Size: 4400
EarI CTCTTCn'nnn_
Cuts at: 2423 2633 2423
Size: 210 4190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1361 2608 2918 3064 4210 1361
Size: 1247 310 146 1146 1551
Fragments arranged by size:
1551 1247 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1412 1412
Size: 4400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 2225 2657 3404 3672 190
Size: 2035 432 747 268 918
Fragments arranged by size:
2035 918 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 730 730
Size: 4400
EcoO109I rG'GnC_Cy
Cuts at: 1539 1539
Size: 4400
EcoRI G'AATT_C
Cuts at: 1404 1404
Size: 4400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 412 2419 412
Size: 2007 2393
FspI TGC'GCA
Cuts at: 2181 2181
Size: 4400
HaeII r_GCGC'y
Cuts at: 655 1834 2082 3104 4083 655
Size: 1179 248 1022 979 972
Fragments arranged by size:
1179 1022 979 972 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 969 1001 1362 1394 2242 2272 2274
Size: 32 750 32 361 32 848 30 2
Cuts at: 2274 2304 187
Size: 30 2283
Fragments arranged by size:
2283 848 750 361 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 312 1439 312
Size: 1127 3273
HpaI GTT'AAC
Cuts at: 312 312
Size: 4400
KasI G'GCGC_C
Cuts at: 1830 2078 1830
Size: 248 4152
KpnI G_GTAC'C
Cuts at: 144 1420 144
Size: 1276 3124
MluI A'CGCG_T
Cuts at: 3833 3833
Size: 4400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3071 3255 3071
Size: 184 4216
MscI TGG'CCA
Cuts at: 157 1108 2161 157
Size: 951 1053 2396
Fragments arranged by size:
2396 1053 951
MslI CAynn'nnrTG
Cuts at: 238 1108 2389 2516 238
Size: 870 1281 127 2122
Fragments arranged by size:
2122 1281 870 127
MspA1I CmG'CkG
Cuts at: 433 1251 1511 1825 2185 3198 3443 433
Size: 818 260 314 360 1013 245 1390
Fragments arranged by size:
1390 1013 818 360 314 260 245
NaeI GCC'GGC
Cuts at: 822 2581 822
Size: 1759 2641
NarI GG'CG_CC
Cuts at: 1831 2079 1831
Size: 248 4152
NcoI C'CATG_G
Cuts at: 1103 1103
Size: 4400
NgoMIV G'CCGG_C
Cuts at: 820 2579 820
Size: 1759 2641
NspI r_CATG'y
Cuts at: 351 1137 1453 1953 2860 351
Size: 786 316 500 907 1891
Fragments arranged by size:
1891 907 786 500 316
PciI A'CATG_T
Cuts at: 2856 2856
Size: 4400
PflMI CCAn_nnn'nTGG
Cuts at: 1284 1284
Size: 4400
PmeI GTTT'AAAC
Cuts at: 1399 1399
Size: 4400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 4368
PshAI GACnn'nnGTC
Cuts at: 485 485
Size: 4400
PstI C_TGCA'G
Cuts at: 813 1119 1447 2132 813
Size: 306 328 685 3081
Fragments arranged by size:
3081 685 328 306
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4400
PvuII CAG'CTG
Cuts at: 433 1251 1825 2185 433
Size: 818 574 360 2648
Fragments arranged by size:
2648 818 574 360
RsrII CG'GwC_CG
Cuts at: 2595 2595
Size: 4400
SacI G_AGCT'C
Cuts at: 1414 1414
Size: 4400
SalI G'TCGA_C
Cuts at: 1437 1437
Size: 4400
SapI GCTCTTCn'nnn_
Cuts at: 2423 2633 2423
Size: 210 4190
SbfI CC_TGCA'GG
Cuts at: 1119 1447 1119
Size: 328 4072
ScaI AGT'ACT
Cuts at: 3917 3917
Size: 4400
SfcI C'TryA_G
Cuts at: 33 809 1115 1443 2128 3121 3312 33
Size: 776 306 328 685 993 191 1121
Fragments arranged by size:
1121 993 776 685 328 306 191
SfoI GGC'GCC
Cuts at: 1832 2080 1832
Size: 248 4152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
SmaI CCC'GGG
Cuts at: 1011 1422 1011
Size: 411 3989
SmlI C'TyrA_G
Cuts at: 329 950 1555 2470 2962 3224 329
Size: 621 605 915 492 262 1505
Fragments arranged by size:
1505 915 621 605 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4400
SphI G_CATG'C
Cuts at: 351 1137 1453 1953 351
Size: 786 316 500 2798
Fragments arranged by size:
2798 786 500 316
StyI C'CwwG_G
Cuts at: 1103 1534 1888 1103
Size: 431 354 3615
Fragments arranged by size:
3615 431 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 497 1117 1141 1192 1983 2116 2575 2784 497
Size: 620 24 51 791 133 459 209 2113
Fragments arranged by size:
2113 791 620 459 209 133 51 24
TatI w'GTAC_w
Cuts at: 3915 3915
Size: 4400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 222 254 3917 3949 222
Size: 32 3663 32 673
Fragments arranged by size:
3663 673 32 32
Tth111I GACn'n_nGTC
Cuts at: 2197 2197
Size: 4400
XbaI T'CTAG_A
Cuts at: 1431 1431
Size: 4400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 165 1225 1285 1895 165
Size: 1060 60 610 2670
Fragments arranged by size:
2670 1060 610 60
XmaI C'CCGG_G
Cuts at: 1009 1420 1009
Size: 411 3989
XmnI GAAnn'nnTTC
Cuts at: 338 548 338
Size: 210 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI