Restriction map for
pF1KB5356
(Circular) (Six-base) MAPSORT of: pF1KB5356.seq Check: 3852 from: 1 to: 4457
pF1KB5356 4457 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 163 163
Size: 4457
AccI GT'mk_AC
Cuts at: 1495 1495
Size: 4457
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 4297 4297
Size: 4457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2714 3067 3729 2282
Size: 432 353 662 3010
Fragments arranged by size:
3010 662 432 353
AflIII A'CryG_T
Cuts at: 1809 3609 3890 1809
Size: 1800 281 2376
Fragments arranged by size:
2376 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1762 3200 1762
Size: 1438 3019
ApaI G_GGCC'C
Cuts at: 220 778 220
Size: 558 3899
ApaLI G'TGCA_C
Cuts at: 3295 3295
Size: 4457
ApoI r'AATT_y
Cuts at: 1461 3810 4066 1461
Size: 2349 256 1852
Fragments arranged by size:
2349 1852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 313 1198 1477 313
Size: 885 279 3293
Fragments arranged by size:
3293 885 279
BamHI G'GATC_C
Cuts at: 1482 1482
Size: 4457
BanI G'GyrC_C
Cuts at: 877 1045 1473 1887 2135 2170 877
Size: 168 428 414 248 35 3164
Fragments arranged by size:
3164 428 414 248 168 35
BanII G_rGCy'C
Cuts at: 184 220 778 968 1379 1471 2501 184
Size: 36 558 190 411 92 1030 2140
Fragments arranged by size:
2140 1030 558 411 190 92 36
BbeI G_GCGC'C
Cuts at: 881 1891 2139 881
Size: 1010 248 3199
Fragments arranged by size:
3199 1010 248
BbsI GAAGACnn'nnnn_
Cuts at: 623 1088 623
Size: 465 3992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4423
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3411 4457 2355
Size: 1056 1046 2355
Fragments arranged by size:
2355 1056 1046
BclI T'GATC_A
Cuts at: 371 542 623 809 1977 371
Size: 171 81 186 1168 2851
Fragments arranged by size:
2851 1168 186 171 81
BglI GCCn_nnn'nGGC
Cuts at: 206 206
Size: 4457
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 1569 1569
Size: 4457
Bme1580I G_kGCm'C
Cuts at: 220 778 1050 2082 2175 3299 220
Size: 558 272 1032 93 1124 1378
Fragments arranged by size:
1378 1124 1032 558 272 93
BmrI ACTGGGnnnn_n'
Cuts at: 237 1669 1750 2085 4229 237
Size: 1432 81 335 2144 465
Fragments arranged by size:
2144 1432 465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1036 1183 352
Size: 684 147 3626
Fragments arranged by size:
3626 684 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1633 2512 3226 3524 667
Size: 966 879 714 298 1600
Fragments arranged by size:
1600 966 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4161 4161
Size: 4457
BsaAI yAC'GTr
Cuts at: 1810 1810
Size: 4457
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 160 878 1888 2136 2838 4334 160
Size: 718 1010 248 702 1496 283
Fragments arranged by size:
1496 1010 718 702 283 248
BsaWI w'CCGG_w
Cuts at: 1646 2167 2845 3256 3403 3677 1646
Size: 521 678 411 147 274 2426
Fragments arranged by size:
2426 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 292 502 1026 1171 1186 280
Size: 12 210 524 145 15 3551
Fragments arranged by size:
3551 524 210 145 15 12
BseYI C'CCAG_C
Cuts at: 1882 2640 3305 3713 1882
Size: 758 665 408 2626
Fragments arranged by size:
2626 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 176 322 1414 1999 2045 3275 104
Size: 72 146 1092 585 46 1230 1286
Fragments arranged by size:
1286 1230 1092 585 146 72 46
BsiHKAI G_wGCw'C
Cuts at: 584 683 1471 2249 2439 3299 584
Size: 99 788 778 190 860 1742
Fragments arranged by size:
1742 860 788 778 190 99
BsmI GAATG_Cn'
Cuts at: 938 938
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 184 220 584 683 778 968 1050 1379 1471
Size: 36 364 99 95 190 82 329 92
Cuts at: 1471 2082 2175 2249 2439 2501 3299 184
Size: 611 93 74 190 62 798 1342
Fragments arranged by size:
1342 798 611 364 329 190 190 99
95 93 92 82 74 62 36
BspHI T'CATG_A
Cuts at: 539 806 2889 539
Size: 267 2083 2107
Fragments arranged by size:
2107 2083 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1507 1721 2023 2404 1507
Size: 214 302 381 3560
Fragments arranged by size:
3560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 156 2749 2803 4287 4452 50
Size: 106 2593 54 1484 165 55
Fragments arranged by size:
2593 1484 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 2369 2369
Size: 4457
BsrFI r'CCGG_y
Cuts at: 2455 2636 2455
Size: 181 4276
BssSI C'ACGA_G
Cuts at: 898 2728 3436 898
Size: 1830 708 1919
Fragments arranged by size:
1919 1830 708
BstAPI GCAn_nnn'nTGC
Cuts at: 758 1834 758
Size: 1076 3381
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 573 573
Size: 4457
BstYI r'GATC_y
Cuts at: 1 1482 1949 1972 2307 2553 2859 2871 2957
Size: 1481 467 23 335 246 306 12 86
Cuts at: 2957 4009 1
Size: 1052 449
Fragments arranged by size:
1481 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 612 701 998 1244 1394 612
Size: 89 297 246 150 3675
Fragments arranged by size:
3675 297 246 150 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 752 1700 752
Size: 948 3509
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DraIII CAC_nnn'GTG
Cuts at: 398 398
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 295 2163 3507 295
Size: 1868 1344 1245
Fragments arranged by size:
1868 1344 1245
EaeI y'GGCC_r
Cuts at: 173 319 698 1286 1411 2042 2216 2607 2634
Size: 146 379 588 125 631 174 391 27
Cuts at: 2634 3979 173
Size: 1345 651
Fragments arranged by size:
1345 651 631 588 391 379 174 146
125 27
EagI C'GGCC_G
Cuts at: 173 319 1411 2042 173
Size: 146 1092 631 2588
Fragments arranged by size:
2588 1092 631 146
EarI CTCTTCn'nnn_
Cuts at: 332 658 1163 2480 2690 332
Size: 326 505 1317 210 2099
Fragments arranged by size:
2099 1317 505 326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2665 3407 3553 4267 2665
Size: 742 146 714 2855
Fragments arranged by size:
2855 742 714 146
EcoICRI GAG'CTC
Cuts at: 1469 1469
Size: 4457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1036 1183 2282 2714 3067 3729 352
Size: 684 147 1099 432 353 662 1080
Fragments arranged by size:
1099 1080 684 662 432 353 147
EcoO109I rG'GnC_Cy
Cuts at: 216 282 774 775 922 1596 216
Size: 66 492 1 147 674 3077
Fragments arranged by size:
3077 674 492 147 66 1
EcoRI G'AATT_C
Cuts at: 1461 1461
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4457
FspI TGC'GCA
Cuts at: 2238 2238
Size: 4457
HaeII r_GCGC'y
Cuts at: 510 881 1345 1891 2139 3369 4140 510
Size: 371 464 546 248 1230 771 827
Fragments arranged by size:
1230 827 771 546 464 371 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 449 478 481 510 1021 1053 2299
Size: 32 272 29 3 29 511 32 1246
Cuts at: 2299 2329 2331 2361 145
Size: 30 2 30 2241
Fragments arranged by size:
2241 1246 511 272 32 32 30 30
29 29 3 2
HincII GTy'rAC
Cuts at: 1496 1496
Size: 4457
HindIII A'AGCT_T
Cuts at: 1189 1189
Size: 4457
KasI G'GCGC_C
Cuts at: 877 1887 2135 877
Size: 1010 248 3199
Fragments arranged by size:
3199 1010 248
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3216 3400 3216
Size: 184 4273
MscI TGG'CCA
Cuts at: 700 1288 2218 700
Size: 588 930 2939
Fragments arranged by size:
2939 930 588
MslI CAynn'nnrTG
Cuts at: 118 406 886 2446 2573 118
Size: 288 480 1560 127 2002
Fragments arranged by size:
2002 1560 480 288 127
MspA1I CmG'CkG
Cuts at: 596 1253 1568 1882 2242 3026 3271 596
Size: 657 315 314 360 784 245 1782
Fragments arranged by size:
1782 784 657 360 315 314 245
NaeI GCC'GGC
Cuts at: 2638 2638
Size: 4457
NarI GG'CG_CC
Cuts at: 878 1888 2136 878
Size: 1010 248 3199
Fragments arranged by size:
3199 1010 248
NcoI C'CATG_G
Cuts at: 612 701 998 612
Size: 89 297 4071
Fragments arranged by size:
4071 297 89
NgoMIV G'CCGG_C
Cuts at: 2636 2636
Size: 4457
NotI GC'GGCC_GC
Cuts at: 319 319
Size: 4457
NspI r_CATG'y
Cuts at: 370 1510 2010 3613 370
Size: 1140 500 1603 1214
Fragments arranged by size:
1603 1214 1140 500
PasI CC'CwG_GG
Cuts at: 1150 1150
Size: 4457
PciI A'CATG_T
Cuts at: 3609 3609
Size: 4457
PflMI CCAn_nnn'nTGG
Cuts at: 226 1004 226
Size: 778 3679
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 4425
PpuMI rG'GwC_Cy
Cuts at: 282 282
Size: 4457
PspOMI G'GGCC_C
Cuts at: 216 774 216
Size: 558 3899
PstI C_TGCA'G
Cuts at: 791 1504 2189 791
Size: 713 685 3059
Fragments arranged by size:
3059 713 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 1882 2242 1882
Size: 360 4097
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 1471 1471
Size: 4457
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 332 2480 2690 332
Size: 2148 210 2099
Fragments arranged by size:
2148 2099 210
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 3974 3974
Size: 4457
SexAI A'CCwGG_T
Cuts at: 1403 1403
Size: 4457
SfcI C'TryA_G
Cuts at: 33 300 787 1215 1500 2185 3153 3344 33
Size: 267 487 428 285 685 968 191 1146
Fragments arranged by size:
1146 968 685 487 428 285 267 191
SfoI GGC'GCC
Cuts at: 879 1889 2137 879
Size: 1010 248 3199
Fragments arranged by size:
3199 1010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 315 1479 315
Size: 1164 3293
SmlI C'TyrA_G
Cuts at: 682 1612 2527 3241 3503 682
Size: 930 915 714 262 1636
Fragments arranged by size:
1636 930 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 1510 2010 1510
Size: 500 3957
StuI AGG'CCT
Cuts at: 329 796 329
Size: 467 3990
StyI C'CwwG_G
Cuts at: 612 669 701 998 1591 1945 612
Size: 57 32 297 593 354 3124
Fragments arranged by size:
3124 593 354 297 57 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2040 2173 2632 2841 2040
Size: 133 459 209 3656
Fragments arranged by size:
3656 459 209 133
TatI w'GTAC_w
Cuts at: 3972 3972
Size: 4457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 162 3974 4006 130
Size: 32 3812 32 581
Fragments arranged by size:
3812 581 32 32
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 573 1401 1952 573
Size: 828 551 3078
Fragments arranged by size:
3078 828 551
XmaI C'CCGG_G
Cuts at: 313 1477 313
Size: 1164 3293
ZraI GAC'GTC
Cuts at: 161 161
Size: 4457
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI