Restriction map for
pF1KB5353
(Circular) (Six-base) MAPSORT of: pF1KB5353.seq Check: 4826 from: 1 to: 4286
pF1KB5353 4286 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1040 1076 2111 2543 3290 3558 706
Size: 334 36 1035 432 747 268 1434
Fragments arranged by size:
1434 1035 747 432 334 268 36
AflIII A'CryG_T
Cuts at: 1638 2742 3719 1638
Size: 1104 977 2205
Fragments arranged by size:
2205 1104 977
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 724 1591 3158 724
Size: 867 1567 1852
Fragments arranged by size:
1852 1567 867
ApaLI G'TGCA_C
Cuts at: 371 3056 371
Size: 2685 1601
ApoI r'AATT_y
Cuts at: 388 605 613 751 1290 3639 3895 388
Size: 217 8 138 539 2349 256 779
Fragments arranged by size:
2349 779 539 256 217 138 8
AseI AT'TA_AT
Cuts at: 20 470 20
Size: 450 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 1306 1306
Size: 4286
AvrII C'CTAG_G
Cuts at: 319 319
Size: 4286
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 878 1302 1716 1964 1999 878
Size: 424 414 248 35 3165
Fragments arranged by size:
3165 424 414 248 35
BanII G_rGCy'C
Cuts at: 1300 2330 1300
Size: 1030 3256
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 599 599
Size: 4286
BciVI GTATCCnnnnn_n'
Cuts at: 295 2184 2945 4286 295
Size: 1889 761 1341 295
Fragments arranged by size:
1889 1341 761 295
BclI T'GATC_A
Cuts at: 144 1806 144
Size: 1662 2624
BglII A'GATC_T
Cuts at: 1038 1801 3838 1038
Size: 763 2037 1486
Fragments arranged by size:
2037 1486 763
BlpI GC'TnA_GC
Cuts at: 1398 1398
Size: 4286
Bme1580I G_kGCm'C
Cuts at: 375 1911 2004 3060 375
Size: 1536 93 1056 1601
Fragments arranged by size:
1601 1536 1056 93
BmgBI CAC'GTC
Cuts at: 214 952 214
Size: 738 3548
BmrI ACTGGGnnnn_n'
Cuts at: 1498 1579 1914 4058 1498
Size: 81 335 2144 1726
Fragments arranged by size:
2144 1726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1157
Size: 4286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2341 2833 3131 1462
Size: 879 492 298 2617
Fragments arranged by size:
2617 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3990 3990
Size: 4286
BsaAI yAC'GTr
Cuts at: 1099 1639 1099
Size: 540 3746
BsaBI GATnn'nnATC
Cuts at: 6 833 1800 3837 6
Size: 827 967 2037 455
Fragments arranged by size:
2037 967 827 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1475 1996 2674 2948 3095 3506 1475
Size: 521 678 274 147 411 2255
Fragments arranged by size:
2255 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1260 1711 2469 3046 3542 1260
Size: 451 758 577 496 2004
Fragments arranged by size:
2004 758 577 496 451
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3082 104
Size: 1724 46 1208 1308
Fragments arranged by size:
1724 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 375 1300 2078 2268 3060 375
Size: 925 778 190 792 1601
Fragments arranged by size:
1601 925 792 778 190
BsmI GAATG_Cn'
Cuts at: 348 348
Size: 4286
Bsp1286I G_dGCh'C
Cuts at: 375 1300 1911 2004 2078 2268 2330 3060 375
Size: 925 611 93 74 190 62 730 1601
Fragments arranged by size:
1601 925 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3506 3506
Size: 4286
BspHI T'CATG_A
Cuts at: 405 3462 405
Size: 3057 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 365 1269 1336 1550 1852 2233 365
Size: 904 67 214 302 381 2418
Fragments arranged by size:
2418 904 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2578 2632 4116 4281 50
Size: 2528 54 1484 165 55
Fragments arranged by size:
2528 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 2284 2465 2674 2284
Size: 181 209 3896
Fragments arranged by size:
3896 209 181
BsrGI T'GTAC_A
Cuts at: 871 871
Size: 4286
BssSI C'ACGA_G
Cuts at: 2557 2915 2557
Size: 358 3928
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1663
Size: 4286
BstBI TT'CG_AA
Cuts at: 1079 2647 1079
Size: 1568 2718
BstYI r'GATC_y
Cuts at: 1 1038 1311 1778 1801 2136 2382 3394 3480
Size: 1037 273 467 23 335 246 1012 86
Cuts at: 3480 3492 3838 1
Size: 12 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 273 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 1529 1529
Size: 4286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 933 968 933
Size: 35 4251
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 278 1992 2850 278
Size: 1714 858 1714
Fragments arranged by size:
1714 1714 858
EaeI y'GGCC_r
Cuts at: 726 1871 2045 2436 2463 3808 726
Size: 1145 174 391 27 1345 1204
Fragments arranged by size:
1345 1204 1145 391 174 27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 173 2309 2519 173
Size: 2136 210 1940
Fragments arranged by size:
2136 1940 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2494 2804 2950 4096 2494
Size: 310 146 1146 2684
Fragments arranged by size:
2684 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1040 1076 1157 2111 2543 3290 3558 706
Size: 334 36 81 954 432 747 268 1434
Fragments arranged by size:
1434 954 747 432 334 268 81 36
EcoO109I rG'GnC_Cy
Cuts at: 1425 1425
Size: 4286
EcoRI G'AATT_C
Cuts at: 605 1290 605
Size: 685 3601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 289 1081 2305 289
Size: 792 1224 2270
Fragments arranged by size:
2270 1224 792
FspI TGC'GCA
Cuts at: 2067 2067
Size: 4286
HaeII r_GCGC'y
Cuts at: 1720 1968 2990 3969 1720
Size: 248 1022 979 2037
Fragments arranged by size:
2037 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 1023 1055 2128 2158 2160 2190 225
Size: 32 766 32 1073 30 2 30 2321
Fragments arranged by size:
2321 1073 766 32 32 30 30 2
HincII GTy'rAC
Cuts at: 623 642 1325 623
Size: 19 683 3584
Fragments arranged by size:
3584 683 19
HpaI GTT'AAC
Cuts at: 623 623
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3141 2957
Size: 184 4102
MscI TGG'CCA
Cuts at: 728 2047 728
Size: 1319 2967
MslI CAynn'nnrTG
Cuts at: 2275 2402 2275
Size: 127 4159
MspA1I CmG'CkG
Cuts at: 1397 1711 2071 3084 3329 1397
Size: 314 360 1013 245 2354
Fragments arranged by size:
2354 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 107 107
Size: 4286
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 1339 1839 2746 1339
Size: 500 907 2879
Fragments arranged by size:
2879 907 500
PciI A'CATG_T
Cuts at: 2742 2742
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4254
PstI C_TGCA'G
Cuts at: 1074 1333 2018 1074
Size: 259 685 3342
Fragments arranged by size:
3342 685 259
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 1711 2071 1711
Size: 360 3926
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 1070 1329 2014 3007 3198 33
Size: 1037 259 685 993 191 1121
Fragments arranged by size:
1121 1037 993 685 259 191
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 1441 2356 2848 3110 1441
Size: 915 492 262 2617
Fragments arranged by size:
2617 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
SspI AAT'ATT
Cuts at: 775 775
Size: 4286
StyI C'CwwG_G
Cuts at: 107 319 1420 1774 107
Size: 212 1101 354 2619
Fragments arranged by size:
2619 1101 354 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1869 2002 2461 2670 1869
Size: 133 459 209 3485
Fragments arranged by size:
3485 459 209 133
TatI w'GTAC_w
Cuts at: 871 3801 871
Size: 2930 1356
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4254
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1781 1781
Size: 4286
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
XmnI GAAnn'nnTTC
Cuts at: 680 680
Size: 4286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI