Restriction map for
pF1KB5352
(Circular) (Six-base) MAPSORT of: pF1KB5352.seq Check: 4963 from: 1 to: 4448
pF1KB5352 4448 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 260 278 1486 260
Size: 18 1208 3222
Fragments arranged by size:
3222 1208 18
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 706 715 2273 2705 3058 3720 343
Size: 363 9 1558 432 353 662 1071
Fragments arranged by size:
1558 1071 662 432 363 353 9
AflIII A'CryG_T
Cuts at: 1169 1800 3600 3881 1169
Size: 631 1800 281 1736
Fragments arranged by size:
1800 1736 631 281
AhdI GACnn_n'nnGTC
Cuts at: 854 1139 854
Size: 285 4163
AlwNI CAG_nnn'CTG
Cuts at: 1753 3191 1753
Size: 1438 3010
ApaLI G'TGCA_C
Cuts at: 3286 3286
Size: 4448
ApoI r'AATT_y
Cuts at: 178 514 988 1224 1452 3801 4057 178
Size: 336 474 236 228 2349 256 569
Fragments arranged by size:
2349 569 474 336 256 236 228
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 1468 1468
Size: 4448
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1140 1173 1140
Size: 33 4415
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 1464 1878 2126 2161 1464
Size: 414 248 35 3751
Fragments arranged by size:
3751 414 248 35
BanII G_rGCy'C
Cuts at: 1032 1462 2492 1032
Size: 430 1030 2988
Fragments arranged by size:
2988 1030 430
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbsI GAAGACnn'nnnn_
Cuts at: 674 674
Size: 4448
BbvCI CC'TCA_GC
Cuts at: 1042 1042
Size: 4448
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3402 4448 2346
Size: 1056 1046 2346
Fragments arranged by size:
2346 1056 1046
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglII A'GATC_T
Cuts at: 933 1963 4000 933
Size: 1030 2037 1381
Fragments arranged by size:
2037 1381 1030
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 2073 2166 3290 2073
Size: 93 1124 3231
Fragments arranged by size:
3231 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1425 1660 1741 2076 4220 1425
Size: 235 81 335 2144 1653
Fragments arranged by size:
2144 1653 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 1181
Size: 4448
Bpu10I CC'TnA_GC
Cuts at: 1042 1042
Size: 4448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1409 1624 2503 3217 3515 1114
Size: 295 215 879 714 298 2047
Fragments arranged by size:
2047 879 714 298 295 215
BsaI GGTCTCn'nnnn_
Cuts at: 682 764 4152 682
Size: 82 3388 978
Fragments arranged by size:
3388 978 82
BsaAI yAC'GTr
Cuts at: 1801 1801
Size: 4448
BsaBI GATnn'nnATC
Cuts at: 6 155 1962 3999 6
Size: 149 1807 2037 455
Fragments arranged by size:
2037 1807 455 149
BsaHI Gr'CG_yC
Cuts at: 1879 2127 2829 4325 1879
Size: 248 702 1496 2002
Fragments arranged by size:
2002 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1637 2158 2836 3247 3394 3668 1637
Size: 521 678 411 147 274 2417
Fragments arranged by size:
2417 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1873 2631 3296 3704 1873
Size: 758 665 408 2617
Fragments arranged by size:
2617 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1990 2036 3266 104
Size: 1886 46 1230 1286
Fragments arranged by size:
1886 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1462 2240 2430 3290 1462
Size: 778 190 860 2620
Fragments arranged by size:
2620 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1123 1123
Size: 4448
Bsp1286I G_dGCh'C
Cuts at: 1032 1462 2073 2166 2240 2430 2492 3290 1032
Size: 430 611 93 74 190 62 798 2190
Fragments arranged by size:
2190 798 611 430 190 93 74 62
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 912 1388 1498 1712 2014 2395 912
Size: 476 110 214 302 381 2965
Fragments arranged by size:
2965 476 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 2446 2627 2446
Size: 181 4267
BssSI C'ACGA_G
Cuts at: 2719 3427 2719
Size: 708 3740
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 909 2809 909
Size: 1900 2548
BstYI r'GATC_y
Cuts at: 1 396 933 1473 1940 1963 2298 2544 2850
Size: 395 537 540 467 23 335 246 306
Cuts at: 2850 2862 2948 4000 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 540 537 467 449 395 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 279 279
Size: 4448
BtgI C'CryG_G
Cuts at: 107 662 107
Size: 555 3893
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 120 2398 2579 2742 120
Size: 2278 181 163 1826
Fragments arranged by size:
2278 1826 181 163
BtsI GCAGTG_nn'
Cuts at: 1691 1691
Size: 4448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 321 356 321
Size: 35 4413
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3498 2154
Size: 1344 3104
EaeI y'GGCC_r
Cuts at: 1361 2033 2207 2598 2625 3970 1361
Size: 672 174 391 27 1345 1839
Fragments arranged by size:
1839 1345 672 391 174 27
EagI C'GGCC_G
Cuts at: 2033 2033
Size: 4448
EarI CTCTTCn'nnn_
Cuts at: 734 817 1126 2471 2681 734
Size: 83 309 1345 210 2501
Fragments arranged by size:
2501 1345 309 210 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 475 2656 3398 3544 4258 127
Size: 348 2181 742 146 714 317
Fragments arranged by size:
2181 742 714 348 317 146
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 706 715 1181 2273 2705 3058 3720 343
Size: 363 9 466 1092 432 353 662 1071
Fragments arranged by size:
1092 1071 662 466 432 363 353 9
EcoO109I rG'GnC_Cy
Cuts at: 1587 1587
Size: 4448
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 1882 2130 3360 4131 1882
Size: 248 1230 771 2199
Fragments arranged by size:
2199 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 840 872 1125 1157 2290 2320 2322 2352 840
Size: 32 253 32 1133 30 2 30 2936
Fragments arranged by size:
2936 1133 253 32 32 30 30 2
HincII GTy'rAC
Cuts at: 425 1011 1487 425
Size: 586 476 3386
Fragments arranged by size:
3386 586 476
HpaI GTT'AAC
Cuts at: 1011 1011
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MfeI C'AATT_G
Cuts at: 377 377
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3207 3391 3207
Size: 184 4264
MscI TGG'CCA
Cuts at: 1363 2209 1363
Size: 846 3602
MslI CAynn'nnrTG
Cuts at: 1168 2437 2564 1168
Size: 1269 127 3052
Fragments arranged by size:
3052 1269 127
MspA1I CmG'CkG
Cuts at: 1559 1873 2233 3017 3262 1559
Size: 314 360 784 245 2745
Fragments arranged by size:
2745 784 360 314 245
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NcoI C'CATG_G
Cuts at: 107 662 107
Size: 555 3893
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NruI TCG'CGA
Cuts at: 794 794
Size: 4448
NspI r_CATG'y
Cuts at: 1173 1501 2001 3604 1173
Size: 328 500 1603 2017
Fragments arranged by size:
2017 1603 500 328
PciI A'CATG_T
Cuts at: 1169 3600 1169
Size: 2431 2017
PflMI CCAn_nnn'nTGG
Cuts at: 1359 1359
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 563 595 2894 2926 563
Size: 32 2299 32 2085
Fragments arranged by size:
2299 2085 32 32
PstI C_TGCA'G
Cuts at: 1495 2180 1495
Size: 685 3763
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1873 2233 1873
Size: 360 4088
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 501 881 1491 2176 3144 3335 33
Size: 468 380 610 685 968 191 1146
Fragments arranged by size:
1146 968 685 610 468 380 191
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 1093 1424 1603 2518 3232 3494 1093
Size: 331 179 915 714 262 2047
Fragments arranged by size:
2047 915 714 331 262 179
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
SspI AAT'ATT
Cuts at: 487 801 1371 487
Size: 314 570 3564
Fragments arranged by size:
3564 570 314
StyI C'CwwG_G
Cuts at: 107 662 1582 1936 107
Size: 555 920 354 2619
Fragments arranged by size:
2619 920 555 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2031 2164 2623 2832 2031
Size: 133 459 209 3647
Fragments arranged by size:
3647 459 209 133
TatI w'GTAC_w
Cuts at: 3963 3963
Size: 4448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 526 1943 526
Size: 1417 3031
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
XmnI GAAnn'nnTTC
Cuts at: 1072 1072
Size: 4448
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstXI Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII NdeI NheI NotI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI