Restriction map for
pF1KB5346
(Circular) (Six-base) MAPSORT of: pF1KB5346.seq Check: 6185 from: 1 to: 4967
pF1KB5346 4967 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1536 1536
Size: 4967
AccI GT'mk_AC
Cuts at: 2005 2005
Size: 4967
Acc65I G'GTAC_C
Cuts at: 1983 1983
Size: 4967
AclI AA'CG_TT
Cuts at: 1561 1961 4807 1561
Size: 400 2846 1721
Fragments arranged by size:
2846 1721 400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 830 1418 1867 2792 3224 3577 4239 286
Size: 544 588 449 925 432 353 662 1014
Fragments arranged by size:
1014 925 662 588 544 449 432 353
AflIII A'CryG_T
Cuts at: 119 2319 4119 4400 119
Size: 2200 1800 281 686
Fragments arranged by size:
2200 1800 686 281
AhdI GACnn_n'nnGTC
Cuts at: 120 120
Size: 4967
AleI CACnn'nnGTG
Cuts at: 130 267 695 130
Size: 137 428 4402
Fragments arranged by size:
4402 428 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 713 713
Size: 4967
AlwNI CAG_nnn'CTG
Cuts at: 210 423 498 825 2272 3710 210
Size: 213 75 327 1447 1438 1467
Fragments arranged by size:
1467 1447 1438 327 213 75
ApaLI G'TGCA_C
Cuts at: 1337 3805 1337
Size: 2468 2499
ApoI r'AATT_y
Cuts at: 1543 1971 4320 4576 1543
Size: 428 2349 256 1934
Fragments arranged by size:
2349 1934 428 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4967
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
AvaI C'yCGr_G
Cuts at: 1204 1885 1987 1204
Size: 681 102 4184
Fragments arranged by size:
4184 681 102
BamHI G'GATC_C
Cuts at: 1992 1992
Size: 4967
BanI G'GyrC_C
Cuts at: 1983 2397 2645 2680 1983
Size: 414 248 35 4270
Fragments arranged by size:
4270 414 248 35
BanII G_rGCy'C
Cuts at: 350 1077 1203 1981 3011 350
Size: 727 126 778 1030 2306
Fragments arranged by size:
2306 1030 778 727 126
BbeI G_GCGC'C
Cuts at: 2401 2649 2401
Size: 248 4719
BbsI GAAGACnn'nnnn_
Cuts at: 152 665 847 1952 152
Size: 513 182 1105 3167
Fragments arranged by size:
3167 1105 513 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 421 455 421
Size: 34 4933
BciVI GTATCCnnnnn_n'
Cuts at: 2865 3921 4967 2865
Size: 1056 1046 2865
Fragments arranged by size:
2865 1056 1046
BclI T'GATC_A
Cuts at: 2487 2487
Size: 4967
BfrBI ATG'CAT
Cuts at: 1392 1392
Size: 4967
BglII A'GATC_T
Cuts at: 1208 2482 4519 1208
Size: 1274 2037 1656
Fragments arranged by size:
2037 1656 1274
BlpI GC'TnA_GC
Cuts at: 2079 2079
Size: 4967
Bme1580I G_kGCm'C
Cuts at: 809 1341 1524 2592 2685 3809 809
Size: 532 183 1068 93 1124 1967
Fragments arranged by size:
1967 1124 1068 532 183 93
BmgBI CAC'GTC
Cuts at: 867 1692 867
Size: 825 4142
BmrI ACTGGGnnnn_n'
Cuts at: 1234 2179 2260 2595 4739 1234
Size: 945 81 335 2144 1462
Fragments arranged by size:
2144 1462 945 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 4967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 355 550 571 847 856 277
Size: 78 195 21 276 9 4388
Fragments arranged by size:
4388 276 195 78 21 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2143 3022 3736 4034 2143
Size: 879 714 298 3076
Fragments arranged by size:
3076 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1589 4671 1589
Size: 3082 1885
BsaAI yAC'GTr
Cuts at: 2320 2320
Size: 4967
BsaBI GATnn'nnATC
Cuts at: 6 2481 4518 6
Size: 2475 2037 455
Fragments arranged by size:
2475 2037 455
BsaHI Gr'CG_yC
Cuts at: 1533 1891 1949 2398 2646 3348 4844 1533
Size: 358 58 449 248 702 1496 1656
Fragments arranged by size:
1656 1496 702 449 358 248 58
BsaWI w'CCGG_w
Cuts at: 323 2156 2677 3355 3766 3913 4187 323
Size: 1833 521 678 411 147 274 1103
Fragments arranged by size:
1833 1103 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 445
Size: 30 4937
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 547 550 319
Size: 228 3 4736
Fragments arranged by size:
4736 228 3
BseYI C'CCAG_C
Cuts at: 713 2392 3150 3815 4223 713
Size: 1679 758 665 408 1457
Fragments arranged by size:
1679 1457 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 719
Size: 4967
BsiEI CG_ry'CG
Cuts at: 104 2509 2555 3785 104
Size: 2405 46 1230 1286
Fragments arranged by size:
2405 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 233 350 1077 1341 1637 1981 2759 2949 3809
Size: 117 727 264 296 344 778 190 860
Cuts at: 3809 233
Size: 1391
Fragments arranged by size:
1391 860 778 727 344 296 264 190
117
BsmI GAATG_Cn'
Cuts at: 402 1417 402
Size: 1015 3952
BsmBI CGTCTCn'nnnn_
Cuts at: 1898 1898
Size: 4967
Bsp1286I G_dGCh'C
Cuts at: 233 350 809 1077 1203 1341 1524 1637 1981
Size: 117 459 268 126 138 183 113 344
Cuts at: 1981 2592 2685 2759 2949 3011 3809 233
Size: 611 93 74 190 62 798 1391
Fragments arranged by size:
1391 798 611 459 344 268 190 183
138 126 117 113 93 74 62
BspEI T'CCGG_A
Cuts at: 323 323
Size: 4967
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 2017 2231 2533 2914 423
Size: 1594 214 302 381 2476
Fragments arranged by size:
2476 1594 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3259 3313 4797 4962 50
Size: 3209 54 1484 165 55
Fragments arranged by size:
3209 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2879 2879
Size: 4967
BsrFI r'CCGG_y
Cuts at: 2965 3146 2965
Size: 181 4786
BsrGI T'GTAC_A
Cuts at: 134 899 1316 134
Size: 765 417 3785
Fragments arranged by size:
3785 765 417
BssSI C'ACGA_G
Cuts at: 1334 1862 3238 3946 1334
Size: 528 1376 708 2355
Fragments arranged by size:
2355 1376 708 528
BstAPI GCAn_nnn'nTGC
Cuts at: 423 498 2344 423
Size: 75 1846 3046
Fragments arranged by size:
3046 1846 75
BstBI TT'CG_AA
Cuts at: 3328 3328
Size: 4967
BstYI r'GATC_y
Cuts at: 1 731 1208 1768 1992 2459 2482 2817 3063
Size: 730 477 560 224 467 23 335 246
Cuts at: 3063 3369 3381 3467 4519 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 730 560 477 467 449 335 306
246 224 86 23 12
BtgI C'CryG_G
Cuts at: 704 704
Size: 4967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2917 3098 3261 2917
Size: 181 163 4623
Fragments arranged by size:
4623 181 163
BtsI GCAGTG_nn'
Cuts at: 702 1788 1830 1941 2210 702
Size: 1086 42 111 269 3459
Fragments arranged by size:
3459 1086 269 111 42
DraI TTT'AAA
Cuts at: 1775 1966 1775
Size: 191 4776
DrdI GACnn_nn'nnGTC
Cuts at: 2673 4017 2673
Size: 1344 3623
EaeI y'GGCC_r
Cuts at: 479 869 1898 2552 2726 3117 3144 4489 479
Size: 390 1029 654 174 391 27 1345 957
Fragments arranged by size:
1345 1029 957 654 391 390 174 27
EagI C'GGCC_G
Cuts at: 2552 2552
Size: 4967
EarI CTCTTCn'nnn_
Cuts at: 499 2990 3200 499
Size: 2491 210 2266
Fragments arranged by size:
2491 2266 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3175 3917 4063 4777 3175
Size: 742 146 714 3365
Fragments arranged by size:
3365 742 714 146
EcoICRI GAG'CTC
Cuts at: 348 1075 1979 348
Size: 727 904 3336
Fragments arranged by size:
3336 904 727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 286 355 550 571 830 847 856 1418
Size: 9 69 195 21 259 17 9 562
Cuts at: 1418 1867 2792 3224 3577 4239 277
Size: 449 925 432 353 662 1005
Fragments arranged by size:
1005 925 662 562 449 432 353 259
195 69 21 17 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 358 1718 358
Size: 1360 3607
EcoO109I rG'GnC_Cy
Cuts at: 822 831 1228 2106 822
Size: 9 397 878 3683
Fragments arranged by size:
3683 878 397 9
EcoRI G'AATT_C
Cuts at: 1971 1971
Size: 4967
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 4967
FspI TGC'GCA
Cuts at: 431 2748 431
Size: 2317 2650
HaeII r_GCGC'y
Cuts at: 792 2401 2649 3879 4650 792
Size: 1609 248 1230 771 1109
Fragments arranged by size:
1609 1230 1109 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 295 327 1317 1349 1423 1455 2809
Size: 32 157 32 990 32 74 32 1354
Cuts at: 2809 2839 2841 2871 106
Size: 30 2 30 2202
Fragments arranged by size:
2202 1354 990 157 74 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2006 2006
Size: 4967
HindIII A'AGCT_T
Cuts at: 160 215 160
Size: 55 4912
KasI G'GCGC_C
Cuts at: 2397 2645 2397
Size: 248 4719
KpnI G_GTAC'C
Cuts at: 1987 1987
Size: 4967
MluI A'CGCG_T
Cuts at: 4400 4400
Size: 4967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 323 1513 3726 3910 134
Size: 189 1190 2213 184 1191
Fragments arranged by size:
2213 1191 1190 189 184
MscI TGG'CCA
Cuts at: 481 871 2728 481
Size: 390 1857 2720
Fragments arranged by size:
2720 1857 390
MslI CAynn'nnrTG
Cuts at: 130 267 395 695 1115 1387 2956 3083 130
Size: 137 128 300 420 272 1569 127 2014
Fragments arranged by size:
2014 1569 420 300 272 137 128 127
MspA1I CmG'CkG
Cuts at: 706 2078 2392 2752 3536 3781 706
Size: 1372 314 360 784 245 1892
Fragments arranged by size:
1892 1372 784 360 314 245
NaeI GCC'GGC
Cuts at: 3148 3148
Size: 4967
NarI GG'CG_CC
Cuts at: 2398 2646 2398
Size: 248 4719
NgoMIV G'CCGG_C
Cuts at: 3146 3146
Size: 4967
NsiI A_TGCA'T
Cuts at: 1394 1394
Size: 4967
NspI r_CATG'y
Cuts at: 123 1396 2020 2520 4123 123
Size: 1273 624 500 1603 967
Fragments arranged by size:
1603 1273 967 624 500
PasI CC'CwG_GG
Cuts at: 667 667
Size: 4967
PciI A'CATG_T
Cuts at: 119 4119 119
Size: 4000 967
PflMI CCAn_nnn'nTGG
Cuts at: 762 762
Size: 4967
PmeI GTTT'AAAC
Cuts at: 1966 1966
Size: 4967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4935
PpuMI rG'GwC_Cy
Cuts at: 831 1228 831
Size: 397 4570
PstI C_TGCA'G
Cuts at: 503 1557 2014 2699 503
Size: 1054 457 685 2771
Fragments arranged by size:
2771 1054 685 457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4967
PvuII CAG'CTG
Cuts at: 2392 2752 2392
Size: 360 4607
RsrII CG'GwC_CG
Cuts at: 3162 3162
Size: 4967
SacI G_AGCT'C
Cuts at: 350 1077 1981 350
Size: 727 904 3336
Fragments arranged by size:
3336 904 727
SacII CC_GC'GG
Cuts at: 707 707
Size: 4967
SalI G'TCGA_C
Cuts at: 2004 2004
Size: 4967
SapI GCTCTTCn'nnn_
Cuts at: 2990 3200 2990
Size: 210 4757
SbfI CC_TGCA'GG
Cuts at: 2014 2014
Size: 4967
ScaI AGT'ACT
Cuts at: 4484 4484
Size: 4967
SexAI A'CCwGG_T
Cuts at: 761 761
Size: 4967
SfcI C'TryA_G
Cuts at: 33 499 1361 1553 2010 2695 3663 3854 33
Size: 466 862 192 457 685 968 191 1146
Fragments arranged by size:
1146 968 862 685 466 457 192 191
SfoI GGC'GCC
Cuts at: 2399 2647 2399
Size: 248 4719
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4967
SmaI CCC'GGG
Cuts at: 1887 1989 1887
Size: 102 4865
SmlI C'TyrA_G
Cuts at: 1204 2122 3037 3751 4013 1204
Size: 918 915 714 262 2158
Fragments arranged by size:
2158 918 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4967
SphI G_CATG'C
Cuts at: 1396 2020 2520 1396
Size: 624 500 3843
Fragments arranged by size:
3843 624 500
SspI AAT'ATT
Cuts at: 1186 1186
Size: 4967
StyI C'CwwG_G
Cuts at: 893 950 1515 2101 2455 893
Size: 57 565 586 354 3405
Fragments arranged by size:
3405 586 565 354 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2550 2683 3142 3351 2550
Size: 133 459 209 4166
Fragments arranged by size:
4166 459 209 133
TatI w'GTAC_w
Cuts at: 134 899 1043 1316 4482 134
Size: 765 144 273 3166 619
Fragments arranged by size:
3166 765 619 273 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 4484 4516 445
Size: 32 4007 32 896
Fragments arranged by size:
4007 896 32 32
Tth111I GACn'n_nGTC
Cuts at: 1693 2764 1693
Size: 1071 3896
XbaI T'CTAG_A
Cuts at: 1998 1998
Size: 4967
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 2462 630
Size: 1832 3135
XhoI C'TCGA_G
Cuts at: 1204 1204
Size: 4967
XmaI C'CCGG_G
Cuts at: 1885 1987 1885
Size: 102 4865
ZraI GAC'GTC
Cuts at: 1534 1534
Size: 4967
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmtI Bpu10I BsiWI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI