Restriction map for
pF1KB5344
(Circular) (Six-base) MAPSORT of: pF1KB5344.seq Check: 4918 from: 1 to: 3809
pF1KB5344 3809 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 847 847
Size: 3809
Acc65I G'GTAC_C
Cuts at: 825 825
Size: 3809
AclI AA'CG_TT
Cuts at: 3649 3649
Size: 3809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1634 2066 2813 3081 1634
Size: 432 747 268 2362
Fragments arranged by size:
2362 747 432 268
AflIII A'CryG_T
Cuts at: 1161 2265 3242 1161
Size: 1104 977 1728
Fragments arranged by size:
1728 1104 977
AgeI A'CCGG_T
Cuts at: 2197 2197
Size: 3809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 581 602 581
Size: 21 3788
AlwNI CAG_nnn'CTG
Cuts at: 1114 2681 1114
Size: 1567 2242
ApaI G_GGCC'C
Cuts at: 126 126
Size: 3809
ApaLI G'TGCA_C
Cuts at: 2579 2579
Size: 3809
ApoI r'AATT_y
Cuts at: 813 3162 3418 813
Size: 2349 256 1204
Fragments arranged by size:
2349 1204 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
AvaI C'yCGr_G
Cuts at: 829 829
Size: 3809
BamHI G'GATC_C
Cuts at: 834 834
Size: 3809
BanI G'GyrC_C
Cuts at: 540 825 1239 1487 1522 540
Size: 285 414 248 35 2827
Fragments arranged by size:
2827 414 285 248 35
BanII G_rGCy'C
Cuts at: 126 703 823 1853 126
Size: 577 120 1030 2082
Fragments arranged by size:
2082 1030 577 120
BbeI G_GCGC'C
Cuts at: 544 1243 1491 544
Size: 699 248 2862
Fragments arranged by size:
2862 699 248
BbsI GAAGACnn'nnnn_
Cuts at: 190 190
Size: 3809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 346 380 346
Size: 34 3775
BciVI GTATCCnnnnn_n'
Cuts at: 300 1707 2468 3809 300
Size: 1407 761 1341 300
Fragments arranged by size:
1407 1341 761 300
BclI T'GATC_A
Cuts at: 1329 1329
Size: 3809
BglII A'GATC_T
Cuts at: 1324 3361 1324
Size: 2037 1772
BlpI GC'TnA_GC
Cuts at: 609 921 609
Size: 312 3497
Bme1580I G_kGCm'C
Cuts at: 126 239 1434 1527 2583 126
Size: 113 1195 93 1056 1352
Fragments arranged by size:
1352 1195 1056 113 93
BmgBI CAC'GTC
Cuts at: 171 171
Size: 3809
BmrI ACTGGGnnnn_n'
Cuts at: 734 1021 1102 1437 3581 734
Size: 287 81 335 2144 962
Fragments arranged by size:
2144 962 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 3809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 985 1864 2356 2654 403
Size: 582 879 492 298 1558
Fragments arranged by size:
1558 879 582 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 220 3513 220
Size: 3293 516
BsaAI yAC'GTr
Cuts at: 1162 1162
Size: 3809
BsaBI GATnn'nnATC
Cuts at: 6 218 1323 3360 6
Size: 212 1105 2037 455
Fragments arranged by size:
2037 1105 455 212
BsaHI Gr'CG_yC
Cuts at: 541 1240 1488 2190 3686 541
Size: 699 248 702 1496 664
Fragments arranged by size:
1496 702 699 664 248
BsaWI w'CCGG_w
Cuts at: 998 1519 2197 2471 2618 3029 998
Size: 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 713 743 713
Size: 30 3779
BseYI C'CCAG_C
Cuts at: 128 454 582 1234 1992 2569 3065 128
Size: 326 128 652 758 577 496 872
Fragments arranged by size:
872 758 652 577 496 326 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 608 193
Size: 415 3394
BsiEI CG_ry'CG
Cuts at: 104 1351 1397 2605 104
Size: 1247 46 1208 1308
Fragments arranged by size:
1308 1247 1208 46
BsiHKAI G_wGCw'C
Cuts at: 823 1601 1791 2583 823
Size: 778 190 792 2049
Fragments arranged by size:
2049 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 111 111
Size: 3809
Bsp1286I G_dGCh'C
Cuts at: 126 239 703 823 1434 1527 1601 1791 1853
Size: 113 464 120 611 93 74 190 62
Cuts at: 1853 2583 126
Size: 730 1352
Fragments arranged by size:
1352 730 611 464 190 120 113 93
74 62
BspEI T'CCGG_A
Cuts at: 3029 3029
Size: 3809
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 859 1073 1375 1756 500
Size: 359 214 302 381 2553
Fragments arranged by size:
2553 381 359 302 214
BsrBI CCG'CTC
Cuts at: 50 2101 2155 3639 3804 50
Size: 2051 54 1484 165 55
Fragments arranged by size:
2051 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1721 1721
Size: 3809
BsrFI r'CCGG_y
Cuts at: 1807 1988 2197 1807
Size: 181 209 3419
Fragments arranged by size:
3419 209 181
BssSI C'ACGA_G
Cuts at: 2080 2438 2080
Size: 358 3451
BstAPI GCAn_nnn'nTGC
Cuts at: 1186 1186
Size: 3809
BstBI TT'CG_AA
Cuts at: 248 2170 248
Size: 1922 1887
BstYI r'GATC_y
Cuts at: 1 316 834 1301 1324 1659 1905 2917 3003
Size: 315 518 467 23 335 246 1012 86
Cuts at: 3003 3015 3361 1
Size: 12 346 449
Fragments arranged by size:
1012 518 467 449 346 335 315 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 312 312
Size: 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 205 1759 1940 2103 205
Size: 1554 181 163 1911
Fragments arranged by size:
1911 1554 181 163
BtsI GCAGTG_nn'
Cuts at: 181 685 1052 181
Size: 504 367 2938
Fragments arranged by size:
2938 504 367
DraI TTT'AAA
Cuts at: 808 808
Size: 3809
DrdI GACnn_nn'nnGTC
Cuts at: 1515 2373 1515
Size: 858 2951
EaeI y'GGCC_r
Cuts at: 140 1394 1568 1959 1986 3331 140
Size: 1254 174 391 27 1345 618
Fragments arranged by size:
1345 1254 618 391 174 27
EagI C'GGCC_G
Cuts at: 1394 1394
Size: 3809
EarI CTCTTCn'nnn_
Cuts at: 691 765 1832 2042 691
Size: 74 1067 210 2458
Fragments arranged by size:
2458 1067 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2017 2327 2473 3619 2017
Size: 310 146 1146 2207
Fragments arranged by size:
2207 1146 310 146
EcoICRI GAG'CTC
Cuts at: 821 821
Size: 3809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1634 2066 2813 3081 1634
Size: 432 747 268 2362
Fragments arranged by size:
2362 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 123 948 123
Size: 825 2984
EcoRI G'AATT_C
Cuts at: 813 813
Size: 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3809
FspI TGC'GCA
Cuts at: 1590 1590
Size: 3809
HaeII r_GCGC'y
Cuts at: 544 1243 1491 2513 3492 544
Size: 699 248 1022 979 861
Fragments arranged by size:
1022 979 861 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 1651 1681 1683 1713 137
Size: 32 1482 30 2 30 2233
Fragments arranged by size:
2233 1482 32 30 30 2
HincII GTy'rAC
Cuts at: 848 848
Size: 3809
KasI G'GCGC_C
Cuts at: 540 1239 1487 540
Size: 699 248 2862
Fragments arranged by size:
2862 699 248
KpnI G_GTAC'C
Cuts at: 829 829
Size: 3809
MluI A'CGCG_T
Cuts at: 3242 3242
Size: 3809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2664 2480
Size: 184 3625
MscI TGG'CCA
Cuts at: 142 1570 142
Size: 1428 2381
MslI CAynn'nnrTG
Cuts at: 1798 1925 1798
Size: 127 3682
MspA1I CmG'CkG
Cuts at: 582 920 1234 1594 2607 2852 582
Size: 338 314 360 1013 245 1539
Fragments arranged by size:
1539 1013 360 338 314 245
NaeI GCC'GGC
Cuts at: 1990 1990
Size: 3809
NarI GG'CG_CC
Cuts at: 541 1240 1488 541
Size: 699 248 2862
Fragments arranged by size:
2862 699 248
NgoMIV G'CCGG_C
Cuts at: 1988 1988
Size: 3809
NspI r_CATG'y
Cuts at: 862 1362 2269 862
Size: 500 907 2402
Fragments arranged by size:
2402 907 500
PciI A'CATG_T
Cuts at: 2265 2265
Size: 3809
PmeI GTTT'AAAC
Cuts at: 808 808
Size: 3809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3777
PspOMI G'GGCC_C
Cuts at: 122 122
Size: 3809
PstI C_TGCA'G
Cuts at: 856 1541 856
Size: 685 3124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3809
PvuII CAG'CTG
Cuts at: 582 1234 1594 582
Size: 652 360 2797
Fragments arranged by size:
2797 652 360
RsrII CG'GwC_CG
Cuts at: 2004 2004
Size: 3809
SacI G_AGCT'C
Cuts at: 823 823
Size: 3809
SalI G'TCGA_C
Cuts at: 846 846
Size: 3809
SapI GCTCTTCn'nnn_
Cuts at: 691 1832 2042 691
Size: 1141 210 2458
Fragments arranged by size:
2458 1141 210
SbfI CC_TGCA'GG
Cuts at: 856 856
Size: 3809
ScaI AGT'ACT
Cuts at: 510 3326 510
Size: 2816 993
SfcI C'TryA_G
Cuts at: 33 351 852 1537 2530 2721 33
Size: 318 501 685 993 191 1121
Fragments arranged by size:
1121 993 685 501 318 191
SfoI GGC'GCC
Cuts at: 542 1241 1489 542
Size: 699 248 2862
Fragments arranged by size:
2862 699 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
SmaI CCC'GGG
Cuts at: 831 831
Size: 3809
SmlI C'TyrA_G
Cuts at: 418 964 1879 2371 2633 418
Size: 546 915 492 262 1594
Fragments arranged by size:
1594 915 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3809
SphI G_CATG'C
Cuts at: 862 1362 862
Size: 500 3309
StyI C'CwwG_G
Cuts at: 943 1297 943
Size: 354 3455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 574 1392 1525 1984 2193 574
Size: 818 133 459 209 2190
Fragments arranged by size:
2190 818 459 209 133
TatI w'GTAC_w
Cuts at: 508 3324 508
Size: 2816 993
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 3326 3358 160
Size: 32 3134 32 611
Fragments arranged by size:
3134 611 32 32
Tth111I GACn'n_nGTC
Cuts at: 1606 1606
Size: 3809
XbaI T'CTAG_A
Cuts at: 840 840
Size: 3809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1304
Size: 3809
XmaI C'CCGG_G
Cuts at: 829 829
Size: 3809
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I
BseRI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI