Restriction map for
pF1KB5335
(Circular) (Six-base) MAPSORT of: pF1KB5335.seq Check: 7711 from: 1 to: 5072
pF1KB5335 5072 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2110 2110
Size: 5072
Acc65I G'GTAC_C
Cuts at: 2088 2088
Size: 5072
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 840 1008 1092 1176 1260 1344 1512 1596
Size: 84 168 84 84 84 84 168 84
Cuts at: 1596 1932 2897 3329 3682 4344 756
Size: 336 965 432 353 662 1484
Fragments arranged by size:
1484 965 662 432 353 336 168 168
84 84 84 84 84 84
AflIII A'CryG_T
Cuts at: 830 998 1082 1754 1933 2424 4224 4505 830
Size: 168 84 672 179 491 1800 281 1397
Fragments arranged by size:
1800 1397 672 491 281 179 168 84
AleI CACnn'nnGTG
Cuts at: 983 983
Size: 5072
AlwNI CAG_nnn'CTG
Cuts at: 1178 2377 3815 1178
Size: 1199 1438 2435
Fragments arranged by size:
2435 1438 1199
ApaI G_GGCC'C
Cuts at: 475 475
Size: 5072
ApaLI G'TGCA_C
Cuts at: 3910 3910
Size: 5072
ApoI r'AATT_y
Cuts at: 230 382 1368 2076 4425 4681 230
Size: 152 986 708 2349 256 621
Fragments arranged by size:
2349 986 708 621 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 5072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 897 1737 2092 897
Size: 840 355 3877
Fragments arranged by size:
3877 840 355
BamHI G'GATC_C
Cuts at: 465 2097 465
Size: 1632 3440
BanI G'GyrC_C
Cuts at: 553 2088 2502 2750 2785 553
Size: 1535 414 248 35 2840
Fragments arranged by size:
2840 1535 414 248 35
BanII G_rGCy'C
Cuts at: 166 341 475 1818 2086 3116 166
Size: 175 134 1343 268 1030 2122
Fragments arranged by size:
2122 1343 1030 268 175 134
BbeI G_GCGC'C
Cuts at: 2506 2754 2506
Size: 248 4824
BbsI GAAGACnn'nnnn_
Cuts at: 1165 1987 1165
Size: 822 4250
BbvCI CC'TCA_GC
Cuts at: 1966 1966
Size: 5072
BciVI GTATCCnnnnn_n'
Cuts at: 800 2970 4026 5072 800
Size: 2170 1056 1046 800
Fragments arranged by size:
2170 1056 1046 800
BclI T'GATC_A
Cuts at: 525 2592 525
Size: 2067 3005
BglII A'GATC_T
Cuts at: 2587 4624 2587
Size: 2037 3035
BlpI GC'TnA_GC
Cuts at: 160 2184 160
Size: 2024 3048
Bme1580I G_kGCm'C
Cuts at: 475 1355 2697 2790 3914 475
Size: 880 1342 93 1124 1633
Fragments arranged by size:
1633 1342 1124 880 93
BmgBI CAC'GTC
Cuts at: 1934 1988 1934
Size: 54 5018
BmrI ACTGGGnnnn_n'
Cuts at: 235 1412 1783 2284 2365 2700 4844 235
Size: 1177 371 501 81 335 2144 463
Fragments arranged by size:
2144 1177 501 463 371 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 185 3439 185
Size: 3254 1818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 286 306 357 540 588 220
Size: 66 20 51 183 48 4704
Fragments arranged by size:
4704 183 66 51 48 20
Bpu10I CC'TnA_GC
Cuts at: 1966 2024 1966
Size: 58 5014
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 1939 2248 3127 3841 4139 1435
Size: 504 309 879 714 298 2368
Fragments arranged by size:
2368 879 714 504 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 225 274 507 1717 4776 225
Size: 49 233 1210 3059 521
Fragments arranged by size:
3059 1210 521 233 49
BsaAI yAC'GTr
Cuts at: 831 1083 2425 831
Size: 252 1342 3478
Fragments arranged by size:
3478 1342 252
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 2503 2751 3453 4949 2503
Size: 248 702 1496 2626
Fragments arranged by size:
2626 1496 702 248
BsaWI w'CCGG_w
Cuts at: 944 982 2261 2782 3460 3871 4018 4292 944
Size: 38 1279 521 678 411 147 274 1724
Fragments arranged by size:
1724 1279 678 521 411 274 147 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1198 1228 1399 1429 1651 1681 1198
Size: 30 171 30 222 30 4589
Fragments arranged by size:
4589 222 171 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1168 1168
Size: 5072
BseYI C'CCAG_C
Cuts at: 260 672 2497 3255 3920 4328 260
Size: 412 1825 758 665 408 1004
Fragments arranged by size:
1825 1004 758 665 412 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 939 1023 1191 1779 1863 2032 2054 461
Size: 478 84 168 588 84 169 22 3479
Fragments arranged by size:
3479 588 478 169 168 84 84 22
BsiEI CG_ry'CG
Cuts at: 104 2614 2660 3890 104
Size: 2510 46 1230 1286
Fragments arranged by size:
2510 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 341 2086 2864 3054 3914 341
Size: 1745 778 190 860 1499
Fragments arranged by size:
1745 1499 860 778 190
BsmI GAATG_Cn'
Cuts at: 112 112
Size: 5072
Bsp1286I G_dGCh'C
Cuts at: 166 341 475 1355 1818 2086 2697 2790 2864
Size: 175 134 880 463 268 611 93 74
Cuts at: 2864 3054 3116 3914 166
Size: 190 62 798 1324
Fragments arranged by size:
1324 880 798 611 463 268 190 175
134 93 74 62
BspHI T'CATG_A
Cuts at: 3504 3504
Size: 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 797 2122 2336 2638 3019 797
Size: 1325 214 302 381 2850
Fragments arranged by size:
2850 1325 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3364 3418 4902 5067 50
Size: 3314 54 1484 165 55
Fragments arranged by size:
3314 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2984 2984
Size: 5072
BsrFI r'CCGG_y
Cuts at: 309 1708 3070 3251 309
Size: 1399 1362 181 2130
Fragments arranged by size:
2130 1399 1362 181
BsrGI T'GTAC_A
Cuts at: 182 182
Size: 5072
BssSI C'ACGA_G
Cuts at: 3343 4051 3343
Size: 708 4364
BstAPI GCAn_nnn'nTGC
Cuts at: 399 2449 399
Size: 2050 3022
BstBI TT'CG_AA
Cuts at: 3433 3433
Size: 5072
BstYI r'GATC_y
Cuts at: 1 324 422 465 2097 2564 2587 2922 3168
Size: 323 98 43 1632 467 23 335 246
Cuts at: 3168 3474 3486 3572 4624 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1632 1052 467 449 335 323 306 246
98 86 43 23 12
Bsu36I CC'TnA_GG
Cuts at: 542 692 542
Size: 150 4922
BtgI C'CryG_G
Cuts at: 107 1583 107
Size: 1476 3596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1284 3022 3203 3366 1284
Size: 1738 181 163 2990
Fragments arranged by size:
2990 1738 181 163
BtsI GCAGTG_nn'
Cuts at: 1011 1179 1771 2315 1011
Size: 168 592 544 3768
Fragments arranged by size:
3768 592 544 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1245 1280 1497 1532 1917 1952 1245
Size: 35 217 35 385 35 4365
Fragments arranged by size:
4365 385 217 35 35 35
DraI TTT'AAA
Cuts at: 2071 2071
Size: 5072
DrdI GACnn_nn'nnGTC
Cuts at: 2778 4122 2778
Size: 1344 3728
EaeI y'GGCC_r
Cuts at: 1518 1854 2657 2831 3222 3249 4594 1518
Size: 336 803 174 391 27 1345 1996
Fragments arranged by size:
1996 1345 803 391 336 174 27
EagI C'GGCC_G
Cuts at: 2657 2657
Size: 5072
EarI CTCTTCn'nnn_
Cuts at: 709 862 1820 3095 3305 709
Size: 153 958 1275 210 2476
Fragments arranged by size:
2476 1275 958 210 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1407 3280 4022 4168 4882 1407
Size: 1873 742 146 714 1597
Fragments arranged by size:
1873 1597 742 714 146
EcoICRI GAG'CTC
Cuts at: 339 2084 339
Size: 1745 3327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 286 306 357 540 588 756 840 1008
Size: 66 20 51 183 48 168 84 168
Cuts at: 1008 1092 1176 1260 1344 1512 1596 1932 2897
Size: 84 84 84 84 168 84 336 965
Cuts at: 2897 3329 3682 4344 220
Size: 432 353 662 948
Fragments arranged by size:
965 948 662 432 353 336 183 168
168 168 84 84 84 84 84 84
66 51 48 20
EcoO109I rG'GnC_Cy
Cuts at: 538 2211 538
Size: 1673 3399
EcoRI G'AATT_C
Cuts at: 1368 2076 1368
Size: 708 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 5072
FspI TGC'GCA
Cuts at: 2853 2853
Size: 5072
HaeII r_GCGC'y
Cuts at: 2506 2754 3984 4755 2506
Size: 248 1230 771 2823
Fragments arranged by size:
2823 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2914 2944 2946 2976 2914
Size: 30 2 30 5010
Fragments arranged by size:
5010 30 30 2
HincII GTy'rAC
Cuts at: 747 953 1299 2111 747
Size: 206 346 812 3708
Fragments arranged by size:
3708 812 346 206
KasI G'GCGC_C
Cuts at: 2502 2750 2502
Size: 248 4824
KpnI G_GTAC'C
Cuts at: 2092 2092
Size: 5072
MfeI C'AATT_G
Cuts at: 911 911
Size: 5072
MluI A'CGCG_T
Cuts at: 4505 4505
Size: 5072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1565 3831 4015 1565
Size: 2266 184 2622
Fragments arranged by size:
2622 2266 184
MscI TGG'CCA
Cuts at: 1856 2833 1856
Size: 977 4095
MslI CAynn'nnrTG
Cuts at: 435 983 1923 3061 3188 435
Size: 548 940 1138 127 2319
Fragments arranged by size:
2319 1138 940 548 127
MspA1I CmG'CkG
Cuts at: 264 804 1123 2183 2497 2857 3641 3886 264
Size: 540 319 1060 314 360 784 245 1450
Fragments arranged by size:
1450 1060 784 540 360 319 314 245
NaeI GCC'GGC
Cuts at: 3253 3253
Size: 5072
NarI GG'CG_CC
Cuts at: 2503 2751 2503
Size: 248 4824
NcoI C'CATG_G
Cuts at: 107 1583 107
Size: 1476 3596
NdeI CA'TA_TG
Cuts at: 1920 1920
Size: 5072
NgoMIV G'CCGG_C
Cuts at: 3251 3251
Size: 5072
NspI r_CATG'y
Cuts at: 400 434 1002 1758 2125 2625 4228 400
Size: 34 568 756 367 500 1603 1244
Fragments arranged by size:
1603 1244 756 568 500 367 34
PasI CC'CwG_GG
Cuts at: 475 475
Size: 5072
PciI A'CATG_T
Cuts at: 998 1754 4224 998
Size: 756 2470 1846
Fragments arranged by size:
2470 1846 756
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1468 1500 3518 3550 1468
Size: 32 2018 32 2990
Fragments arranged by size:
2990 2018 32 32
PpuMI rG'GwC_Cy
Cuts at: 538 538
Size: 5072
PspOMI G'GGCC_C
Cuts at: 471 471
Size: 5072
PspXI vC'TCGA_Gb
Cuts at: 1737 1737
Size: 5072
PstI C_TGCA'G
Cuts at: 2119 2804 2119
Size: 685 4387
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5072
PvuII CAG'CTG
Cuts at: 264 1123 2497 2857 264
Size: 859 1374 360 2479
Fragments arranged by size:
2479 1374 859 360
RsrII CG'GwC_CG
Cuts at: 3267 3267
Size: 5072
SacI G_AGCT'C
Cuts at: 341 2086 341
Size: 1745 3327
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SapI GCTCTTCn'nnn_
Cuts at: 862 3095 3305 862
Size: 2233 210 2629
Fragments arranged by size:
2629 2233 210
SbfI CC_TGCA'GG
Cuts at: 2119 2119
Size: 5072
ScaI AGT'ACT
Cuts at: 4589 4589
Size: 5072
SexAI A'CCwGG_T
Cuts at: 215 215
Size: 5072
SfcI C'TryA_G
Cuts at: 33 207 2115 2800 3768 3959 33
Size: 174 1908 685 968 191 1146
Fragments arranged by size:
1908 1146 968 685 191 174
SfoI GGC'GCC
Cuts at: 2504 2752 2504
Size: 248 4824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SmaI CCC'GGG
Cuts at: 2094 2094
Size: 5072
SmlI C'TyrA_G
Cuts at: 1450 1737 1954 2227 3142 3856 4118 1450
Size: 287 217 273 915 714 262 2404
Fragments arranged by size:
2404 915 714 287 273 262 217
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5072
SphI G_CATG'C
Cuts at: 2125 2625 2125
Size: 500 4572
StyI C'CwwG_G
Cuts at: 107 1583 1857 2206 2560 107
Size: 1476 274 349 354 2619
Fragments arranged by size:
2619 1476 354 349 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 274 2655 2788 3247 3456 154
Size: 120 2381 133 459 209 1770
Fragments arranged by size:
2381 1770 459 209 133 120
TatI w'GTAC_w
Cuts at: 182 4587 182
Size: 4405 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4589 4621 4589
Size: 32 5040
Tth111I GACn'n_nGTC
Cuts at: 1986 2869 1986
Size: 883 4189
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2567 2567
Size: 5072
XhoI C'TCGA_G
Cuts at: 1737 1737
Size: 5072
XmaI C'CCGG_G
Cuts at: 2092 2092
Size: 5072
XmnI GAAnn'nnTTC
Cuts at: 853 853
Size: 5072
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmBI BspEI BssHII BstEII BstXI BstZ17I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI