Restriction map for
pF1KB5334
(Circular) (Six-base) MAPSORT of: pF1KB5334.seq Check: 1808 from: 1 to: 4679
pF1KB5334 4679 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 994 2504 2936 3289 3951 151
Size: 843 1510 432 353 662 879
Fragments arranged by size:
1510 879 843 662 432 353
AflIII A'CryG_T
Cuts at: 2031 3831 4112 2031
Size: 1800 281 2598
Fragments arranged by size:
2598 1800 281
AleI CACnn'nnGTG
Cuts at: 862 1218 862
Size: 356 4323
AlwNI CAG_nnn'CTG
Cuts at: 207 1984 3422 207
Size: 1777 1438 1464
Fragments arranged by size:
1777 1464 1438
ApaLI G'TGCA_C
Cuts at: 213 3517 213
Size: 3304 1375
ApoI r'AATT_y
Cuts at: 540 1683 4032 4288 540
Size: 1143 2349 256 931
Fragments arranged by size:
2349 1143 931 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 157 1699 157
Size: 1542 3137
AvrII C'CTAG_G
Cuts at: 1399 1399
Size: 4679
BamHI G'GATC_C
Cuts at: 267 1704 267
Size: 1437 3242
BanI G'GyrC_C
Cuts at: 153 754 1695 2109 2357 2392 153
Size: 601 941 414 248 35 2440
Fragments arranged by size:
2440 941 601 414 248 35
BanII G_rGCy'C
Cuts at: 1693 2723 1693
Size: 1030 3649
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BciVI GTATCCnnnnn_n'
Cuts at: 1058 2577 3633 4679 1058
Size: 1519 1056 1046 1058
Fragments arranged by size:
1519 1058 1056 1046
BclI T'GATC_A
Cuts at: 2199 2199
Size: 4679
BfrBI ATG'CAT
Cuts at: 825 825
Size: 4679
BglII A'GATC_T
Cuts at: 1005 2194 4231 1005
Size: 1189 2037 1453
Fragments arranged by size:
2037 1453 1189
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 158 217 589 757 2304 2397 3521 158
Size: 59 372 168 1547 93 1124 1316
Fragments arranged by size:
1547 1316 1124 372 168 93 59
BmrI ACTGGGnnnn_n'
Cuts at: 489 1891 1972 2307 4451 489
Size: 1402 81 335 2144 717
Fragments arranged by size:
2144 1402 717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 435 861 3046 435
Size: 426 2185 2068
Fragments arranged by size:
2185 2068 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 299 385 1412 169
Size: 130 86 1027 3436
Fragments arranged by size:
3436 1027 130 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1855 2734 3448 3746 1855
Size: 879 714 298 2788
Fragments arranged by size:
2788 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 652 1475 1536 4383 283
Size: 369 823 61 2847 579
Fragments arranged by size:
2847 823 579 369 61
BsaAI yAC'GTr
Cuts at: 2032 2032
Size: 4679
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 1519 2110 2358 3060 4556 1519
Size: 591 248 702 1496 1642
Fragments arranged by size:
1642 1496 702 591 248
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3478 3625 3899 1868
Size: 521 678 411 147 274 2648
Fragments arranged by size:
2648 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 174 1522 1633 2104 2862 3527 3935 174
Size: 1348 111 471 758 665 408 918
Fragments arranged by size:
1348 918 758 665 471 408 111
BsiEI CG_ry'CG
Cuts at: 104 2221 2267 3497 104
Size: 2117 46 1230 1286
Fragments arranged by size:
2117 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 217 1693 2471 2661 3521 217
Size: 1476 778 190 860 1375
Fragments arranged by size:
1476 1375 860 778 190
BsmI GAATG_Cn'
Cuts at: 390 648 390
Size: 258 4421
BsmBI CGTCTCn'nnnn_
Cuts at: 154 1510 154
Size: 1356 3323
Bsp1286I G_dGCh'C
Cuts at: 158 217 589 757 1693 2304 2397 2471 2661
Size: 59 372 168 936 611 93 74 190
Cuts at: 2661 2723 3521 158
Size: 62 798 1316
Fragments arranged by size:
1316 936 798 611 372 190 168 93
74 62 59
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1729 1943 2245 2626 1729
Size: 214 302 381 3782
Fragments arranged by size:
3782 381 302 214
BsrBI CCG'CTC
Cuts at: 50 800 2971 3025 4509 4674 50
Size: 750 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 750 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2591 2591
Size: 4679
BsrFI r'CCGG_y
Cuts at: 1658 2677 2858 1658
Size: 1019 181 3479
Fragments arranged by size:
3479 1019 181
BsrGI T'GTAC_A
Cuts at: 1292 1292
Size: 4679
BssSI C'ACGA_G
Cuts at: 2950 3658 2950
Size: 708 3971
BstAPI GCAn_nnn'nTGC
Cuts at: 207 822 2056 207
Size: 615 1234 2830
Fragments arranged by size:
2830 1234 615
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstXI CCAn_nnnn'nTGG
Cuts at: 1220 1220
Size: 4679
BstYI r'GATC_y
Cuts at: 1 267 1005 1133 1704 2171 2194 2529 2775
Size: 266 738 128 571 467 23 335 246
Cuts at: 2775 3081 3093 3179 4231 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 738 571 467 449 335 306 266
246 128 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 196 749 196
Size: 553 4126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1922 1922
Size: 4679
DraI TTT'AAA
Cuts at: 1032 1678 1032
Size: 646 4033
DrdI GACnn_nn'nnGTC
Cuts at: 1258 2385 3729 1258
Size: 1127 1344 2208
Fragments arranged by size:
2208 1344 1127
EaeI y'GGCC_r
Cuts at: 142 425 1645 1656 2264 2438 2829 2856 4201
Size: 283 1220 11 608 174 391 27 1345
Cuts at: 4201 142
Size: 620
Fragments arranged by size:
1345 1220 620 608 391 283 174 27
11
EagI C'GGCC_G
Cuts at: 2264 2264
Size: 4679
EarI CTCTTCn'nnn_
Cuts at: 281 1294 2702 2912 281
Size: 1013 1408 210 2048
Fragments arranged by size:
2048 1408 1013 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3629 3775 4489 2887
Size: 742 146 714 3077
Fragments arranged by size:
3077 742 714 146
EcoICRI GAG'CTC
Cuts at: 1691 1691
Size: 4679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 169 299 385 994 1412 2504 2936 3289
Size: 18 130 86 609 418 1092 432 353
Cuts at: 3289 3951 151
Size: 662 879
Fragments arranged by size:
1092 879 662 609 432 418 353 130
86 18
EcoO109I rG'GnC_Cy
Cuts at: 529 1576 1818 529
Size: 1047 242 3390
Fragments arranged by size:
3390 1047 242
EcoRI G'AATT_C
Cuts at: 1683 1683
Size: 4679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4679
FspI TGC'GCA
Cuts at: 572 2460 572
Size: 1888 2791
FspAI rTGC'GCAy
Cuts at: 572 572
Size: 4679
HaeII r_GCGC'y
Cuts at: 361 2113 2361 3591 4362 361
Size: 1752 248 1230 771 678
Fragments arranged by size:
1752 1230 771 678 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 651 683 1472 1504 2521 2551 2553 2583 651
Size: 32 789 32 1017 30 2 30 2747
Fragments arranged by size:
2747 1017 789 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1361 1718 1361
Size: 357 4322
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 1164 1458 3438 3622 710
Size: 454 294 1980 184 1767
Fragments arranged by size:
1980 1767 454 294 184
MscI TGG'CCA
Cuts at: 427 2440 427
Size: 2013 2666
MslI CAynn'nnrTG
Cuts at: 862 1218 2668 2795 862
Size: 356 1450 127 2746
Fragments arranged by size:
2746 1450 356 127
MspA1I CmG'CkG
Cuts at: 147 676 942 1041 1790 2104 2464 3248 3493
Size: 529 266 99 749 314 360 784 245
Cuts at: 3493 147
Size: 1333
Fragments arranged by size:
1333 784 749 529 360 314 266 245
99
NaeI GCC'GGC
Cuts at: 1660 2860 1660
Size: 1200 3479
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NdeI CA'TA_TG
Cuts at: 303 575 303
Size: 272 4407
NgoMIV G'CCGG_C
Cuts at: 1658 2858 1658
Size: 1200 3479
NsiI A_TGCA'T
Cuts at: 827 827
Size: 4679
NspI r_CATG'y
Cuts at: 825 1732 2232 3835 825
Size: 907 500 1603 1669
Fragments arranged by size:
1669 1603 907 500
PciI A'CATG_T
Cuts at: 3831 3831
Size: 4679
PflMI CCAn_nnn'nTGG
Cuts at: 1654 1654
Size: 4679
PfoI T'CCnGG_A
Cuts at: 1428 1572 1428
Size: 144 4535
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 599 631 3125 3157 599
Size: 32 2494 32 2121
Fragments arranged by size:
2494 2121 32 32
PpuMI rG'GwC_Cy
Cuts at: 1576 1576
Size: 4679
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1112 1144 1112
Size: 32 4647
PstI C_TGCA'G
Cuts at: 818 1726 2411 818
Size: 908 685 3086
Fragments arranged by size:
3086 908 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 676 942 1041 2104 2464 676
Size: 266 99 1063 360 2891
Fragments arranged by size:
2891 1063 360 266 99
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1693 1693
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 2702 2912 2702
Size: 210 4469
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SfcI C'TryA_G
Cuts at: 33 618 814 1722 2407 3375 3566 33
Size: 585 196 908 685 968 191 1146
Fragments arranged by size:
1146 968 908 685 585 196 191
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 1834 2749 3463 3725 1834
Size: 915 714 262 2788
Fragments arranged by size:
2788 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StyI C'CwwG_G
Cuts at: 293 480 1399 1813 2167 293
Size: 187 919 414 354 2805
Fragments arranged by size:
2805 919 414 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2262 2395 2854 3063 2262
Size: 133 459 209 3878
Fragments arranged by size:
3878 459 209 133
TatI w'GTAC_w
Cuts at: 818 1292 4194 818
Size: 474 2902 1303
Fragments arranged by size:
2902 1303 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 4647
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BspEI BssHII
BstEII BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI