Restriction map for
pF1KB5332
(Circular) (Six-base) MAPSORT of: pF1KB5332.seq Check: 3430 from: 1 to: 3938
pF1KB5332 3938 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 976 976
Size: 3938
Acc65I G'GTAC_C
Cuts at: 954 954
Size: 3938
AclI AA'CG_TT
Cuts at: 3778 3778
Size: 3938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1763 2195 2942 3210 1763
Size: 432 747 268 2491
Fragments arranged by size:
2491 747 432 268
AflIII A'CryG_T
Cuts at: 373 447 1290 2394 3371 373
Size: 74 843 1104 977 940
Fragments arranged by size:
1104 977 940 843 74
AgeI A'CCGG_T
Cuts at: 2326 2326
Size: 3938
AlwNI CAG_nnn'CTG
Cuts at: 582 1243 2810 582
Size: 661 1567 1710
Fragments arranged by size:
1710 1567 661
ApaLI G'TGCA_C
Cuts at: 2708 2708
Size: 3938
ApoI r'AATT_y
Cuts at: 292 942 3291 3547 292
Size: 650 2349 256 683
Fragments arranged by size:
2349 683 650 256
AseI AT'TA_AT
Cuts at: 20 316 20
Size: 296 3642
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
AvaI C'yCGr_G
Cuts at: 362 391 458 482 706 958 362
Size: 29 67 24 224 252 3342
Fragments arranged by size:
3342 252 224 67 29 24
BamHI G'GATC_C
Cuts at: 963 963
Size: 3938
BanI G'GyrC_C
Cuts at: 954 1368 1616 1651 954
Size: 414 248 35 3241
Fragments arranged by size:
3241 414 248 35
BanII G_rGCy'C
Cuts at: 149 952 1982 149
Size: 803 1030 2105
Fragments arranged by size:
2105 1030 803
BbeI G_GCGC'C
Cuts at: 1372 1620 1372
Size: 248 3690
BbsI GAAGACnn'nnnn_
Cuts at: 210 440 210
Size: 230 3708
BbvCI CC'TCA_GC
Cuts at: 227 227
Size: 3938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 343 377 90
Size: 34 219 34 3651
Fragments arranged by size:
3651 219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1836 2597 3938 1836
Size: 761 1341 1836
Fragments arranged by size:
1836 1341 761
BclI T'GATC_A
Cuts at: 1458 1458
Size: 3938
BglII A'GATC_T
Cuts at: 1453 3490 1453
Size: 2037 1901
BlpI GC'TnA_GC
Cuts at: 1050 1050
Size: 3938
Bme1580I G_kGCm'C
Cuts at: 1563 1656 2712 1563
Size: 93 1056 2789
Fragments arranged by size:
2789 1056 93
BmgBI CAC'GTC
Cuts at: 455 455
Size: 3938
BmrI ACTGGGnnnn_n'
Cuts at: 261 1150 1231 1566 3710 261
Size: 889 81 335 2144 489
Fragments arranged by size:
2144 889 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 3938
Bpu10I CC'TnA_GC
Cuts at: 227 227
Size: 3938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1993 2485 2783 1114
Size: 879 492 298 2269
Fragments arranged by size:
2269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 455 3642 455
Size: 3187 751
BsaAI yAC'GTr
Cuts at: 534 1291 534
Size: 757 3181
BsaBI GATnn'nnATC
Cuts at: 6 1452 3489 6
Size: 1446 2037 455
Fragments arranged by size:
2037 1446 455
BsaHI Gr'CG_yC
Cuts at: 1369 1617 2319 3815 1369
Size: 248 702 1496 1492
Fragments arranged by size:
1496 1492 702 248
BsaWI w'CCGG_w
Cuts at: 492 1127 1648 2326 2600 2747 3158 492
Size: 635 521 678 274 147 411 1272
Fragments arranged by size:
1272 678 635 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 3908
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 394
Size: 3938
BseYI C'CCAG_C
Cuts at: 1363 2121 2698 3194 1363
Size: 758 577 496 2107
Fragments arranged by size:
2107 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 354
Size: 3938
BsiEI CG_ry'CG
Cuts at: 104 1480 1526 2734 104
Size: 1376 46 1208 1308
Fragments arranged by size:
1376 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 400 952 1730 1920 2712 149
Size: 251 552 778 190 792 1375
Fragments arranged by size:
1375 792 778 552 251 190
BsiWI C'GTAC_G
Cuts at: 113 113
Size: 3938
BsmBI CGTCTCn'nnnn_
Cuts at: 461 461
Size: 3938
Bsp1286I G_dGCh'C
Cuts at: 149 400 952 1563 1656 1730 1920 1982 2712
Size: 251 552 611 93 74 190 62 730
Cuts at: 2712 149
Size: 1375
Fragments arranged by size:
1375 730 611 552 251 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3158 3158
Size: 3938
BspHI T'CATG_A
Cuts at: 285 3114 285
Size: 2829 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 988 1202 1504 1885 988
Size: 214 302 381 3041
Fragments arranged by size:
3041 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2230 2284 3768 3933 50
Size: 2180 54 1484 165 55
Fragments arranged by size:
2180 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 296 435 1850 296
Size: 139 1415 2384
Fragments arranged by size:
2384 1415 139
BsrFI r'CCGG_y
Cuts at: 1936 2117 2326 1936
Size: 181 209 3548
Fragments arranged by size:
3548 209 181
BssSI C'ACGA_G
Cuts at: 901 2209 2567 901
Size: 1308 358 2272
Fragments arranged by size:
2272 1308 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1315 1315
Size: 3938
BstBI TT'CG_AA
Cuts at: 2299 2299
Size: 3938
BstYI r'GATC_y
Cuts at: 1 905 963 1430 1453 1788 2034 3046 3132
Size: 904 58 467 23 335 246 1012 86
Cuts at: 3132 3144 3490 1
Size: 12 346 449
Fragments arranged by size:
1012 904 467 449 346 335 246 86
58 23 12
Bsu36I CC'TnA_GG
Cuts at: 682 682
Size: 3938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 207 1888 2069 2232 207
Size: 1681 181 163 1913
Fragments arranged by size:
1913 1681 181 163
BtsI GCAGTG_nn'
Cuts at: 1181 1181
Size: 3938
DraI TTT'AAA
Cuts at: 937 937
Size: 3938
DraIII CAC_nnn'GTG
Cuts at: 693 693
Size: 3938
DrdI GACnn_nn'nnGTC
Cuts at: 160 1644 2502 160
Size: 1484 858 1596
Fragments arranged by size:
1596 1484 858
EaeI y'GGCC_r
Cuts at: 197 715 1523 1697 2088 2115 3460 197
Size: 518 808 174 391 27 1345 675
Fragments arranged by size:
1345 808 675 518 391 174 27
EagI C'GGCC_G
Cuts at: 1523 1523
Size: 3938
EarI CTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2146 2456 2602 3748 2146
Size: 310 146 1146 2336
Fragments arranged by size:
2336 1146 310 146
EcoICRI GAG'CTC
Cuts at: 147 950 147
Size: 803 3135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1763 2195 2942 3210 1763
Size: 432 747 268 2491
Fragments arranged by size:
2491 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 644 644
Size: 3938
EcoO109I rG'GnC_Cy
Cuts at: 1077 1077
Size: 3938
EcoRI G'AATT_C
Cuts at: 292 942 292
Size: 650 3288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3938
FspI TGC'GCA
Cuts at: 1719 1719
Size: 3938
HaeII r_GCGC'y
Cuts at: 1372 1620 2642 3621 1372
Size: 248 1022 979 1689
Fragments arranged by size:
1689 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 452 454 484 486 736 768 1780 1810 1812
Size: 2 30 2 250 32 1012 30 2
Cuts at: 1812 1842 452
Size: 30 2548
Fragments arranged by size:
2548 1012 250 32 30 30 30 2
2 2
HincII GTy'rAC
Cuts at: 977 977
Size: 3938
KasI G'GCGC_C
Cuts at: 1368 1616 1368
Size: 248 3690
KpnI G_GTAC'C
Cuts at: 958 958
Size: 3938
MluI A'CGCG_T
Cuts at: 3371 3371
Size: 3938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 2609 2793 497
Size: 2112 184 1642
Fragments arranged by size:
2112 1642 184
MscI TGG'CCA
Cuts at: 199 717 1699 199
Size: 518 982 2438
Fragments arranged by size:
2438 982 518
MslI CAynn'nnrTG
Cuts at: 493 1927 2054 493
Size: 1434 127 2377
Fragments arranged by size:
2377 1434 127
MspA1I CmG'CkG
Cuts at: 1049 1363 1723 2736 2981 1049
Size: 314 360 1013 245 2006
Fragments arranged by size:
2006 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 3938
NarI GG'CG_CC
Cuts at: 1369 1617 1369
Size: 248 3690
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 3938
NspI r_CATG'y
Cuts at: 377 451 628 991 1491 2398 377
Size: 74 177 363 500 907 1917
Fragments arranged by size:
1917 907 500 363 177 74
PciI A'CATG_T
Cuts at: 373 447 2394 373
Size: 74 1947 1917
Fragments arranged by size:
1947 1917 74
PflMI CCAn_nnn'nTGG
Cuts at: 251 251
Size: 3938
PfoI T'CCnGG_A
Cuts at: 502 502
Size: 3938
PmeI GTTT'AAAC
Cuts at: 937 937
Size: 3938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3906
PstI C_TGCA'G
Cuts at: 356 648 985 1670 356
Size: 292 337 685 2624
Fragments arranged by size:
2624 685 337 292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3938
PvuII CAG'CTG
Cuts at: 1363 1723 1363
Size: 360 3578
RsrII CG'GwC_CG
Cuts at: 2133 2133
Size: 3938
SacI G_AGCT'C
Cuts at: 149 952 149
Size: 803 3135
SalI G'TCGA_C
Cuts at: 975 975
Size: 3938
SapI GCTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
SbfI CC_TGCA'GG
Cuts at: 985 985
Size: 3938
ScaI AGT'ACT
Cuts at: 358 730 3455 358
Size: 372 2725 841
Fragments arranged by size:
2725 841 372
SfcI C'TryA_G
Cuts at: 33 352 644 981 1666 2659 2850 33
Size: 319 292 337 685 993 191 1121
Fragments arranged by size:
1121 993 685 337 319 292 191
SfoI GGC'GCC
Cuts at: 1370 1618 1370
Size: 248 3690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
SmaI CCC'GGG
Cuts at: 484 960 484
Size: 476 3462
SmlI C'TyrA_G
Cuts at: 458 1093 2008 2500 2762 458
Size: 635 915 492 262 1634
Fragments arranged by size:
1634 915 635 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3938
SphI G_CATG'C
Cuts at: 628 991 1491 628
Size: 363 500 3075
Fragments arranged by size:
3075 500 363
StyI C'CwwG_G
Cuts at: 1072 1426 1072
Size: 354 3584
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1521 1654 2113 2322 1521
Size: 133 459 209 3137
Fragments arranged by size:
3137 459 209 133
TatI w'GTAC_w
Cuts at: 356 728 3453 356
Size: 372 2725 841
Fragments arranged by size:
2725 841 372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 3906
Tth111I GACn'n_nGTC
Cuts at: 1735 1735
Size: 3938
XbaI T'CTAG_A
Cuts at: 969 969
Size: 3938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 531 1433 531
Size: 902 3036
XhoI C'TCGA_G
Cuts at: 458 458
Size: 3938
XmaI C'CCGG_G
Cuts at: 482 958 482
Size: 476 3462
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BglI BmtI BpmI
BsmI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI