Restriction map for
pF1KB5331
(Circular) (Six-base) MAPSORT of: pF1KB5331.seq Check: 9880 from: 1 to: 4358
pF1KB5331 4358 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1178 1178
Size: 4358
AccI GT'mk_AC
Cuts at: 1193 1396 1193
Size: 203 4155
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4358
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 2183 2615 3362 3630 922
Size: 1261 432 747 268 1650
Fragments arranged by size:
1650 1261 747 432 268
AflIII A'CryG_T
Cuts at: 674 815 1710 2814 3791 674
Size: 141 895 1104 977 1241
Fragments arranged by size:
1241 1104 977 895 141
AgeI A'CCGG_T
Cuts at: 319 2746 319
Size: 2427 1931
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 206 1066 206
Size: 860 3498
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1084 1116 1084
Size: 32 4326
AlwNI CAG_nnn'CTG
Cuts at: 201 1663 3230 201
Size: 1462 1567 1329
Fragments arranged by size:
1567 1462 1329
ApaI G_GGCC'C
Cuts at: 231 1322 231
Size: 1091 3267
ApaLI G'TGCA_C
Cuts at: 3128 3128
Size: 4358
ApoI r'AATT_y
Cuts at: 1362 3711 3967 1362
Size: 2349 256 1753
Fragments arranged by size:
2349 1753 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 1378 1378
Size: 4358
AvrII C'CTAG_G
Cuts at: 945 945
Size: 4358
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 1374 1788 2036 2071 1374
Size: 414 248 35 3661
Fragments arranged by size:
3661 414 248 35
BanII G_rGCy'C
Cuts at: 231 704 956 1322 1372 2402 231
Size: 473 252 366 50 1030 2187
Fragments arranged by size:
2187 1030 473 366 252 50
BbeI G_GCGC'C
Cuts at: 1792 2040 1792
Size: 248 4110
BbsI GAAGACnn'nnnn_
Cuts at: 1170 1277 1170
Size: 107 4251
BciVI GTATCCnnnnn_n'
Cuts at: 1293 2256 3017 4358 1293
Size: 963 761 1341 1293
Fragments arranged by size:
1341 1293 963 761
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 587 1470 587
Size: 883 3475
Bme1580I G_kGCm'C
Cuts at: 231 1322 1983 2076 3132 231
Size: 1091 661 93 1056 1457
Fragments arranged by size:
1457 1091 1056 661 93
BmgBI CAC'GTC
Cuts at: 675 873 1086 675
Size: 198 213 3947
Fragments arranged by size:
3947 213 198
BmrI ACTGGGnnnn_n'
Cuts at: 225 1570 1651 1986 4130 225
Size: 1345 81 335 2144 453
Fragments arranged by size:
2144 1345 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1075 595
Size: 480 3878
Bpu10I CC'TnA_GC
Cuts at: 523 523
Size: 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 2905 3203 1534
Size: 879 492 298 2689
Fragments arranged by size:
2689 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4062 4062
Size: 4358
BsaAI yAC'GTr
Cuts at: 818 1711 818
Size: 893 3465
BsaBI GATnn'nnATC
Cuts at: 6 208 1872 3909 6
Size: 202 1664 2037 455
Fragments arranged by size:
2037 1664 455 202
BsaHI Gr'CG_yC
Cuts at: 1175 1789 2037 2739 4235 1175
Size: 614 248 702 1496 1298
Fragments arranged by size:
1496 1298 702 614 248
BsaWI w'CCGG_w
Cuts at: 319 1547 2068 2746 3020 3167 3578 319
Size: 1228 521 678 274 147 411 1099
Fragments arranged by size:
1228 1099 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 664 694 664
Size: 30 4328
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 236 583 1249 1267 1270 1273 1276 1279 236
Size: 347 666 18 3 3 3 3 3315
Fragments arranged by size:
3315 666 347 18 3 3 3 3
BseYI C'CCAG_C
Cuts at: 276 704 1282 1315 1783 2541 3118 3614 276
Size: 428 578 33 468 758 577 496 1020
Fragments arranged by size:
1020 758 578 577 496 468 428 33
BsiEI CG_ry'CG
Cuts at: 104 1900 1946 3154 104
Size: 1796 46 1208 1308
Fragments arranged by size:
1796 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 973 1372 2150 2340 3132 973
Size: 399 778 190 792 2199
Fragments arranged by size:
2199 792 778 399 190
BsmI GAATG_Cn'
Cuts at: 770 770
Size: 4358
Bsp1286I G_dGCh'C
Cuts at: 231 704 956 973 1322 1372 1983 2076 2150
Size: 473 252 17 349 50 611 93 74
Cuts at: 2150 2340 2402 3132 231
Size: 190 62 730 1457
Fragments arranged by size:
1457 730 611 473 349 252 190 93
74 62 50 17
BspEI T'CCGG_A
Cuts at: 3578 3578
Size: 4358
BspHI T'CATG_A
Cuts at: 3534 3534
Size: 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 323 1408 1622 1924 2305 323
Size: 1085 214 302 381 2376
Fragments arranged by size:
2376 1085 381 302 214
BsrBI CCG'CTC
Cuts at: 50 579 2650 2704 4188 4353 50
Size: 529 2071 54 1484 165 55
Fragments arranged by size:
2071 1484 529 165 55 54
BsrDI GCAATG_nn'
Cuts at: 549 938 2270 549
Size: 389 1332 2637
Fragments arranged by size:
2637 1332 389
BsrFI r'CCGG_y
Cuts at: 319 476 973 2356 2537 2746 319
Size: 157 497 1383 181 209 1931
Fragments arranged by size:
1931 1383 497 209 181 157
BssHII G'CGCG_C
Cuts at: 1018 1020 1018
Size: 2 4356
BssSI C'ACGA_G
Cuts at: 1117 1304 2629 2987 1117
Size: 187 1325 358 2488
Fragments arranged by size:
2488 1325 358 187
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1735
Size: 4358
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstEII G'GTnAC_C
Cuts at: 174 396 174
Size: 222 4136
BstYI r'GATC_y
Cuts at: 1 807 1383 1850 1873 2208 2454 3466 3552
Size: 806 576 467 23 335 246 1012 86
Cuts at: 3552 3564 3910 1
Size: 12 346 449
Fragments arranged by size:
1012 806 576 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 323 2308 2489 2652 323
Size: 1985 181 163 2029
Fragments arranged by size:
2029 1985 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 1601 1163
Size: 438 3920
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DrdI GACnn_nn'nnGTC
Cuts at: 2064 2922 2064
Size: 858 3500
EaeI y'GGCC_r
Cuts at: 1943 2117 2508 2535 3880 1943
Size: 174 391 27 1345 2421
Fragments arranged by size:
2421 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4358
EarI CTCTTCn'nnn_
Cuts at: 131 2381 2591 131
Size: 2250 210 1898
Fragments arranged by size:
2250 1898 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 2566 2876 3022 4168 439
Size: 2127 310 146 1146 629
Fragments arranged by size:
2127 1146 629 310 146
EcoICRI GAG'CTC
Cuts at: 1370 1370
Size: 4358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 922 1075 2183 2615 3362 3630 595
Size: 327 153 1108 432 747 268 1323
Fragments arranged by size:
1323 1108 747 432 327 268 153
EcoNI CCTnn'n_nnAGG
Cuts at: 184 1228 184
Size: 1044 3314
EcoO109I rG'GnC_Cy
Cuts at: 227 1319 1497 227
Size: 1092 178 3088
Fragments arranged by size:
3088 1092 178
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 1042 2139 1042
Size: 1097 3261
HaeII r_GCGC'y
Cuts at: 1116 1792 2040 3062 4041 1116
Size: 676 248 1022 979 1433
Fragments arranged by size:
1433 1022 979 676 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 664 696 874 906 2200 2230 2232 2262 664
Size: 32 178 32 1294 30 2 30 2760
Fragments arranged by size:
2760 1294 178 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1397 1397
Size: 4358
KasI G'GCGC_C
Cuts at: 1788 2036 1788
Size: 248 4110
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4358
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3029 3213 3029
Size: 184 4174
MscI TGG'CCA
Cuts at: 2119 2119
Size: 4358
MslI CAynn'nnrTG
Cuts at: 2347 2474 2347
Size: 127 4231
MspA1I CmG'CkG
Cuts at: 304 586 712 935 1107 1282 1469 1783 2143
Size: 282 126 223 172 175 187 314 360
Cuts at: 2143 3156 3401 304
Size: 1013 245 1261
Fragments arranged by size:
1261 1013 360 314 282 245 223 187
175 172 126
NaeI GCC'GGC
Cuts at: 2539 2539
Size: 4358
NarI GG'CG_CC
Cuts at: 1789 2037 1789
Size: 248 4110
NgoMIV G'CCGG_C
Cuts at: 2537 2537
Size: 4358
NspI r_CATG'y
Cuts at: 444 1411 1911 2818 444
Size: 967 500 907 1984
Fragments arranged by size:
1984 967 907 500
PciI A'CATG_T
Cuts at: 2814 2814
Size: 4358
PfoI T'CCnGG_A
Cuts at: 836 1088 836
Size: 252 4106
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 4326
PspOMI G'GGCC_C
Cuts at: 227 1318 227
Size: 1091 3267
PstI C_TGCA'G
Cuts at: 185 422 1112 1405 2090 185
Size: 237 690 293 685 2453
Fragments arranged by size:
2453 690 685 293 237
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 304 712 935 1107 1282 1783 2143 304
Size: 408 223 172 175 501 360 2519
Fragments arranged by size:
2519 501 408 360 223 175 172
RsrII CG'GwC_CG
Cuts at: 581 2553 581
Size: 1972 2386
SacI G_AGCT'C
Cuts at: 1372 1372
Size: 4358
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 2381 2591 2381
Size: 210 4148
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 235 1138 3875 235
Size: 903 2737 718
Fragments arranged by size:
2737 903 718
SfcI C'TryA_G
Cuts at: 33 181 418 1108 1401 2086 3079 3270 33
Size: 148 237 690 293 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 293 237 191 148
SfoI GGC'GCC
Cuts at: 1790 2038 1790
Size: 248 4110
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SgrAI Cr'CCGG_yG
Cuts at: 319 319
Size: 4358
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4358
SmlI C'TyrA_G
Cuts at: 1513 2428 2920 3182 1513
Size: 915 492 262 2689
Fragments arranged by size:
2689 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
StyI C'CwwG_G
Cuts at: 284 945 1322 1492 1846 284
Size: 661 377 170 354 2796
Fragments arranged by size:
2796 661 377 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1941 2074 2533 2742 1941
Size: 133 459 209 3557
Fragments arranged by size:
3557 459 209 133
TatI w'GTAC_w
Cuts at: 233 1136 3873 233
Size: 903 2737 718
Fragments arranged by size:
2737 903 718
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4326
Tth111I GACn'n_nGTC
Cuts at: 2155 2155
Size: 4358
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1853 1853
Size: 4358
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4358
ZraI GAC'GTC
Cuts at: 1176 1176
Size: 4358
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AscI BaeI BbvCI
BcgI BfrBI BglI BmtI BsgI BsiWI BsmBI BsrGI
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI