Restriction map for
pF1KB5326
(Circular) (Six-base) MAPSORT of: pF1KB5326.seq Check: 4990 from: 1 to: 4463
pF1KB5326 4463 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 242 1501 242
Size: 1259 3204
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 2288 2720 3467 3735 721
Size: 1567 432 747 268 1449
Fragments arranged by size:
1567 1449 747 432 268
AfeI AGC'GCT
Cuts at: 725 725
Size: 4463
AflII C'TTAA_G
Cuts at: 775 775
Size: 4463
AflIII A'CryG_T
Cuts at: 1815 2919 3896 1815
Size: 1104 977 2382
Fragments arranged by size:
2382 1104 977
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AleI CACnn'nnGTG
Cuts at: 785 785
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 181 1768 3335 181
Size: 1587 1567 1309
Fragments arranged by size:
1587 1567 1309
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 433 511 1300 1415 1467 3816 4072 433
Size: 78 789 115 52 2349 256 824
Fragments arranged by size:
2349 824 789 256 115 78 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 130 1483 130
Size: 1353 3110
BamHI G'GATC_C
Cuts at: 546 1488 546
Size: 942 3521
BanI G'GyrC_C
Cuts at: 117 150 1479 1893 2141 2176 117
Size: 33 1329 414 248 35 2404
Fragments arranged by size:
2404 1329 414 248 35 33
BanII G_rGCy'C
Cuts at: 172 235 1477 2507 172
Size: 63 1242 1030 2128
Fragments arranged by size:
2128 1242 1030 63
BbeI G_GCGC'C
Cuts at: 154 1897 2145 154
Size: 1743 248 2472
Fragments arranged by size:
2472 1743 248
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BfrBI ATG'CAT
Cuts at: 967 967
Size: 4463
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 2088 2181 3237 2088
Size: 93 1056 3314
Fragments arranged by size:
3314 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 698 1157 1675 1756 2091 4235 698
Size: 459 518 81 335 2144 926
Fragments arranged by size:
2144 926 518 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 696 1193 127
Size: 569 497 3397
Fragments arranged by size:
3397 569 497
Bpu10I CC'TnA_GC
Cuts at: 1103 1436 1103
Size: 333 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 1639 2518 3010 3308 615
Size: 1024 879 492 298 1770
Fragments arranged by size:
1770 1024 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4167 4167
Size: 4463
BsaAI yAC'GTr
Cuts at: 1816 1816
Size: 4463
BsaBI GATnn'nnATC
Cuts at: 6 283 1977 4014 6
Size: 277 1694 2037 455
Fragments arranged by size:
2037 1694 455 277
BsaHI Gr'CG_yC
Cuts at: 151 1894 2142 2844 4340 151
Size: 1743 248 702 1496 274
Fragments arranged by size:
1743 1496 702 274 248
BsaWI w'CCGG_w
Cuts at: 123 1652 2173 2851 3125 3272 3683 123
Size: 1529 521 678 274 147 411 903
Fragments arranged by size:
1529 903 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 165 181 186 1888 2646 3223 3719 165
Size: 16 5 1702 758 577 496 909
Fragments arranged by size:
1702 909 758 577 496 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1080 1080
Size: 4463
BsiEI CG_ry'CG
Cuts at: 104 200 2005 2051 3259 104
Size: 96 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 96 46
BsiHKAI G_wGCw'C
Cuts at: 235 1477 2255 2445 3237 235
Size: 1242 778 190 792 1461
Fragments arranged by size:
1461 1242 792 778 190
BsmI GAATG_Cn'
Cuts at: 1447 1447
Size: 4463
Bsp1286I G_dGCh'C
Cuts at: 172 235 1477 2088 2181 2255 2445 2507 3237
Size: 63 1242 611 93 74 190 62 730
Cuts at: 3237 172
Size: 1398
Fragments arranged by size:
1398 1242 730 611 190 93 74 63
62
BspEI T'CCGG_A
Cuts at: 123 3683 123
Size: 3560 903
BspHI T'CATG_A
Cuts at: 3639 3639
Size: 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1513 1727 2029 2410 1513
Size: 214 302 381 3566
Fragments arranged by size:
3566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 135 2755 2809 4293 4458 50
Size: 85 2620 54 1484 165 55
Fragments arranged by size:
2620 1484 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 1045 2375 1045
Size: 1330 3133
BsrFI r'CCGG_y
Cuts at: 147 2461 2642 2851 147
Size: 2314 181 209 1759
Fragments arranged by size:
2314 1759 209 181
BssSI C'ACGA_G
Cuts at: 2734 3092 2734
Size: 358 4105
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstYI r'GATC_y
Cuts at: 1 546 1488 1955 1978 2313 2559 3571 3657
Size: 545 942 467 23 335 246 1012 86
Cuts at: 3657 3669 4015 1
Size: 12 346 449
Fragments arranged by size:
1012 942 545 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 1135 1706 1135
Size: 571 3892
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 2048 2222 2613 2640 3985 2048
Size: 174 391 27 1345 2526
Fragments arranged by size:
2526 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 221 2486 2696 221
Size: 2265 210 1988
Fragments arranged by size:
2265 1988 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2671 2981 3127 4273 2671
Size: 310 146 1146 2861
Fragments arranged by size:
2861 1146 310 146
EcoICRI GAG'CTC
Cuts at: 233 1475 233
Size: 1242 3221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 696 721 1193 2288 2720 3467 3735 127
Size: 569 25 472 1095 432 747 268 855
Fragments arranged by size:
1095 855 747 569 472 432 268 25
EcoO109I rG'GnC_Cy
Cuts at: 1368 1602 1368
Size: 234 4229
EcoRI G'AATT_C
Cuts at: 1415 1467 1415
Size: 52 4411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1045 1461 2482 1045
Size: 416 1021 3026
Fragments arranged by size:
3026 1021 416
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 154 727 1897 2145 3167 4146 154
Size: 573 1170 248 1022 979 471
Fragments arranged by size:
1170 1022 979 573 471 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 617 649 930 962 1086 1099 1118 1131 2305
Size: 32 281 32 124 13 19 13 1174
Cuts at: 2305 2335 2337 2367 617
Size: 30 2 30 2713
Fragments arranged by size:
2713 1174 281 124 32 32 30 30
19 13 13 2
HincII GTy'rAC
Cuts at: 243 1502 243
Size: 1259 3204
KasI G'GCGC_C
Cuts at: 150 1893 2141 150
Size: 1743 248 2472
Fragments arranged by size:
2472 1743 248
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3134 3318 3134
Size: 184 4279
MscI TGG'CCA
Cuts at: 2224 2224
Size: 4463
MslI CAynn'nnrTG
Cuts at: 331 386 785 956 2452 2579 331
Size: 55 399 171 1496 127 2215
Fragments arranged by size:
2215 1496 399 171 127 55
MspA1I CmG'CkG
Cuts at: 165 699 1574 1888 2248 3261 3506 165
Size: 534 875 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 875 534 360 314 245
NaeI GCC'GGC
Cuts at: 149 2644 149
Size: 2495 1968
NarI GG'CG_CC
Cuts at: 151 1894 2142 151
Size: 1743 248 2472
Fragments arranged by size:
2472 1743 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4463
NdeI CA'TA_TG
Cuts at: 953 953
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 147 2642 147
Size: 2495 1968
NsiI A_TGCA'T
Cuts at: 969 969
Size: 4463
NspI r_CATG'y
Cuts at: 336 1516 2016 2923 336
Size: 1180 500 907 1876
Fragments arranged by size:
1876 1180 907 500
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PflMI CCAn_nnn'nTGG
Cuts at: 181 181
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PstI C_TGCA'G
Cuts at: 800 938 1510 2195 800
Size: 138 572 685 3068
Fragments arranged by size:
3068 685 572 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 699 1888 2248 699
Size: 1189 360 2914
Fragments arranged by size:
2914 1189 360
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 235 1477 235
Size: 1242 3221
SalI G'TCGA_C
Cuts at: 241 1500 241
Size: 1259 3204
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SfcI C'TryA_G
Cuts at: 33 783 796 934 1506 2191 3184 3375 33
Size: 750 13 138 572 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 572 191 138 13
SfoI GGC'GCC
Cuts at: 152 1895 2143 152
Size: 1743 248 2472
Fragments arranged by size:
2472 1743 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 594 775 1618 2533 3025 3287 594
Size: 181 843 915 492 262 1770
Fragments arranged by size:
1770 915 843 492 262 181
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
StyI C'CwwG_G
Cuts at: 107 155 1597 1951 107
Size: 48 1442 354 2619
Fragments arranged by size:
2619 1442 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 913 2046 2179 2638 2847 913
Size: 1133 133 459 209 2529
Fragments arranged by size:
2529 1133 459 209 133
TatI w'GTAC_w
Cuts at: 867 3978 867
Size: 3111 1352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 3980 4012 599
Size: 32 3349 32 1050
Fragments arranged by size:
3349 1050 32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1958 1958
Size: 4463
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
XmnI GAAnn'nnTTC
Cuts at: 587 587
Size: 4463
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI