Restriction map for
pF1KB5316
(Circular) (Six-base) MAPSORT of: pF1KB5316.seq Check: 7735 from: 1 to: 4463
pF1KB5316 4463 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 2288 2720 3073 3735 191
Size: 2097 432 353 662 919
Fragments arranged by size:
2097 919 662 432 353
AflIII A'CryG_T
Cuts at: 1815 3615 3896 1815
Size: 1800 281 2382
Fragments arranged by size:
2382 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1768 3206 1768
Size: 1438 3025
ApaLI G'TGCA_C
Cuts at: 1382 3301 1382
Size: 1919 2544
ApoI r'AATT_y
Cuts at: 480 517 638 731 877 1049 1065 1102 1422
Size: 37 121 93 146 172 16 37 320
Cuts at: 1422 1467 3816 4072 480
Size: 45 2349 256 871
Fragments arranged by size:
2349 871 320 256 172 146 121 93
45 37 37 16
AseI AT'TA_AT
Cuts at: 20 443 1213 20
Size: 423 770 3270
Fragments arranged by size:
3270 770 423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 1483 1483
Size: 4463
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 1479 1893 2141 2176 1479
Size: 414 248 35 3766
Fragments arranged by size:
3766 414 248 35
BanII G_rGCy'C
Cuts at: 1477 2507 1477
Size: 1030 3433
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 935 969 935
Size: 34 4429
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3417 4463 2361
Size: 1056 1046 2361
Fragments arranged by size:
2361 1056 1046
BclI T'GATC_A
Cuts at: 1335 1983 1335
Size: 648 3815
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 1386 2088 2181 3305 1386
Size: 702 93 1124 2544
Fragments arranged by size:
2544 1124 702 93
BmrI ACTGGGnnnn_n'
Cuts at: 1675 1756 2091 4235 1675
Size: 81 335 2144 1903
Fragments arranged by size:
2144 1903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 885 1639 2518 3232 3530 885
Size: 754 879 714 298 1818
Fragments arranged by size:
1818 879 754 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4167 4167
Size: 4463
BsaAI yAC'GTr
Cuts at: 1816 1816
Size: 4463
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 1894 2142 2844 4340 1894
Size: 248 702 1496 2017
Fragments arranged by size:
2017 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1652 2173 2851 3262 3409 3683 1652
Size: 521 678 411 147 274 2432
Fragments arranged by size:
2432 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 160
Size: 4463
BseYI C'CCAG_C
Cuts at: 1888 2646 3311 3719 1888
Size: 758 665 408 2632
Fragments arranged by size:
2632 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1318 1379 431
Size: 887 61 3515
Fragments arranged by size:
3515 887 61
BsiEI CG_ry'CG
Cuts at: 104 2005 2051 3281 104
Size: 1901 46 1230 1286
Fragments arranged by size:
1901 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1386 1477 2255 2445 3305 1386
Size: 91 778 190 860 2544
Fragments arranged by size:
2544 860 778 190 91
Bsp1286I G_dGCh'C
Cuts at: 1386 1477 2088 2181 2255 2445 2507 3305 1386
Size: 91 611 93 74 190 62 798 2544
Fragments arranged by size:
2544 798 611 190 93 91 74 62
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1513 1727 2029 2410 1513
Size: 214 302 381 3566
Fragments arranged by size:
3566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2755 2809 4293 4458 50
Size: 2705 54 1484 165 55
Fragments arranged by size:
2705 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1064 2375 1064
Size: 1311 3152
BsrFI r'CCGG_y
Cuts at: 2461 2642 2461
Size: 181 4282
BsrGI T'GTAC_A
Cuts at: 740 740
Size: 4463
BssSI C'ACGA_G
Cuts at: 2734 3442 2734
Size: 708 3755
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstYI r'GATC_y
Cuts at: 1 1488 1955 1978 2313 2559 2865 2877 2963
Size: 1487 467 23 335 246 306 12 86
Cuts at: 2963 4015 1
Size: 1052 449
Fragments arranged by size:
1487 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 152 2413 2594 2757 152
Size: 2261 181 163 1858
Fragments arranged by size:
2261 1858 181 163
BtsI GCAGTG_nn'
Cuts at: 1306 1706 1306
Size: 400 4063
DraI TTT'AAA
Cuts at: 315 1462 315
Size: 1147 3316
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3513 2169
Size: 1344 3119
EaeI y'GGCC_r
Cuts at: 1326 2048 2222 2613 2640 3985 1326
Size: 722 174 391 27 1345 1804
Fragments arranged by size:
1804 1345 722 391 174 27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 1166 1366 2486 2696 1166
Size: 200 1120 210 2933
Fragments arranged by size:
2933 1120 210 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2671 3413 3559 4273 2671
Size: 742 146 714 2861
Fragments arranged by size:
2861 742 714 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 2288 2720 3073 3735 191
Size: 2097 432 353 662 919
Fragments arranged by size:
2097 919 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1602 1602
Size: 4463
EcoRI G'AATT_C
Cuts at: 480 517 1467 480
Size: 37 950 3476
Fragments arranged by size:
3476 950 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 360 2482 360
Size: 2122 2341
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 1897 2145 3375 4146 1897
Size: 248 1230 771 2214
Fragments arranged by size:
2214 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 448 480 2305 2335 2337 2367 166
Size: 32 250 32 1825 30 2 30 2262
Fragments arranged by size:
2262 1825 250 32 32 30 30 2
HincII GTy'rAC
Cuts at: 353 1344 1502 353
Size: 991 158 3314
Fragments arranged by size:
3314 991 158
HindIII A'AGCT_T
Cuts at: 539 589 628 539
Size: 50 39 4374
Fragments arranged by size:
4374 50 39
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3222 3406 3222
Size: 184 4279
MscI TGG'CCA
Cuts at: 2224 2224
Size: 4463
MslI CAynn'nnrTG
Cuts at: 2452 2579 2452
Size: 127 4336
MspA1I CmG'CkG
Cuts at: 1574 1888 2248 3032 3277 1574
Size: 314 360 784 245 2760
Fragments arranged by size:
2760 784 360 314 245
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NspI r_CATG'y
Cuts at: 1516 2016 3619 1516
Size: 500 1603 2360
Fragments arranged by size:
2360 1603 500
PacI TTA_AT'TAA
Cuts at: 1217 1217
Size: 4463
PciI A'CATG_T
Cuts at: 3615 3615
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 4431
PsiI TTA'TAA
Cuts at: 1207 1207
Size: 4463
PstI C_TGCA'G
Cuts at: 515 1510 2195 515
Size: 995 685 2783
Fragments arranged by size:
2783 995 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 1888 2248 1888
Size: 360 4103
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 1166 2486 2696 1166
Size: 1320 210 2933
Fragments arranged by size:
2933 1320 210
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 214 3980 214
Size: 3766 697
SfcI C'TryA_G
Cuts at: 33 511 1439 1506 2191 3159 3350 33
Size: 478 928 67 685 968 191 1146
Fragments arranged by size:
1146 968 928 685 478 191 67
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 864 1618 2533 3247 3509 864
Size: 754 915 714 262 1818
Fragments arranged by size:
1818 915 754 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
SspI AAT'ATT
Cuts at: 964 964
Size: 4463
StyI C'CwwG_G
Cuts at: 1597 1951 1597
Size: 354 4109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2046 2179 2638 2847 2046
Size: 133 459 209 3662
Fragments arranged by size:
3662 459 209 133
TatI w'GTAC_w
Cuts at: 212 740 3978 212
Size: 528 3238 697
Fragments arranged by size:
3238 697 528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3980 4012 3980
Size: 32 4431
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 673 1958 114
Size: 559 1285 2619
Fragments arranged by size:
2619 1285 559
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
XmnI GAAnn'nnTTC
Cuts at: 426 1112 426
Size: 686 3777
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PacI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI
BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI