Restriction map for
pF1KB5309
(Circular) (Six-base) MAPSORT of: pF1KB5309.seq Check: 9986 from: 1 to: 4382
pF1KB5309 4382 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 279 798 279
Size: 519 3863
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 2207 2639 2992 3654 913
Size: 1294 432 353 662 1641
Fragments arranged by size:
1641 1294 662 432 353
AflIII A'CryG_T
Cuts at: 1734 3534 3815 1734
Size: 1800 281 2301
Fragments arranged by size:
2301 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 276 394 499 547 276
Size: 118 105 48 4111
Fragments arranged by size:
4111 118 105 48
AlwNI CAG_nnn'CTG
Cuts at: 1052 1209 1308 1687 3125 1052
Size: 157 99 379 1438 2309
Fragments arranged by size:
2309 1438 379 157 99
ApaLI G'TGCA_C
Cuts at: 3220 3220
Size: 4382
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AscI GG'CGCG_CC
Cuts at: 993 993
Size: 4382
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 277 499 975 1294 1402 277
Size: 222 476 319 108 3257
Fragments arranged by size:
3257 476 319 222 108
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 564 648 1213 1321 1398 1812 2060 2095 564
Size: 84 565 108 77 414 248 35 2851
Fragments arranged by size:
2851 565 414 248 108 84 77 35
BanII G_rGCy'C
Cuts at: 1396 2426 1396
Size: 1030 3352
BbeI G_GCGC'C
Cuts at: 1325 1816 2064 1325
Size: 491 248 3643
Fragments arranged by size:
3643 491 248
BbsI GAAGACnn'nnnn_
Cuts at: 358 358
Size: 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 432 466 475 509 90
Size: 34 308 34 9 34 3963
Fragments arranged by size:
3963 308 34 34 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3336 4382 2280
Size: 1056 1046 2280
Fragments arranged by size:
2280 1056 1046
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglI GCCn_nnn'nGGC
Cuts at: 229 229
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 212 653 1216 2007 2100 3224 212
Size: 441 563 791 93 1124 1370
Fragments arranged by size:
1370 1124 791 563 441 93
BmgBI CAC'GTC
Cuts at: 924 924
Size: 4382
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 937 847
Size: 90 4292
Bpu10I CC'TnA_GC
Cuts at: 146 146
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1225 1558 2437 3151 3449 403
Size: 822 333 879 714 298 1336
Fragments arranged by size:
1336 879 822 714 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 659 1735 659
Size: 1076 3306
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1166 1322 1813 2061 2763 4259 1166
Size: 156 491 248 702 1496 1289
Fragments arranged by size:
1496 1289 702 491 248 156
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3181 3328 3602 1571
Size: 521 678 411 147 274 2351
Fragments arranged by size:
2351 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 159 796 908 1209 1807 2565 3230 3638 159
Size: 637 112 301 598 758 665 408 903
Fragments arranged by size:
903 758 665 637 598 408 301 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 505 832 257
Size: 248 327 3807
Fragments arranged by size:
3807 327 248
BsiEI CG_ry'CG
Cuts at: 104 441 674 1924 1970 3200 104
Size: 337 233 1250 46 1230 1286
Fragments arranged by size:
1286 1250 1230 337 233 46
BsiHKAI G_wGCw'C
Cuts at: 596 821 857 929 1396 2174 2364 3224 596
Size: 225 36 72 467 778 190 860 1754
Fragments arranged by size:
1754 860 778 467 225 190 72 36
Bsp1286I G_dGCh'C
Cuts at: 212 596 653 821 857 929 1216 1396 2007
Size: 384 57 168 36 72 287 180 611
Cuts at: 2007 2100 2174 2364 2426 3224 212
Size: 93 74 190 62 798 1370
Fragments arranged by size:
1370 798 611 384 287 190 180 168
93 74 72 62 57 36
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 279 764 798 908 1432 1646 1948 2329 279
Size: 485 34 110 524 214 302 381 2332
Fragments arranged by size:
2332 524 485 381 302 214 110 34
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 450 2294 450
Size: 1844 2538
BsrFI r'CCGG_y
Cuts at: 223 413 642 673 1277 2380 2561 223
Size: 190 229 31 604 1103 181 2044
Fragments arranged by size:
2044 1103 604 229 190 181 31
BssHII G'CGCG_C
Cuts at: 164 993 164
Size: 829 3553
BssSI C'ACGA_G
Cuts at: 2653 3361 2653
Size: 708 3674
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstEII G'GTnAC_C
Cuts at: 1335 1335
Size: 4382
BstYI r'GATC_y
Cuts at: 1 1298 1407 1874 1897 2232 2478 2784 2796
Size: 1297 109 467 23 335 246 306 12
Cuts at: 2796 2882 3934 1
Size: 86 1052 449
Fragments arranged by size:
1297 1052 467 449 335 306 246 109
86 23 12
BtgI C'CryG_G
Cuts at: 140 328 967 1160 140
Size: 188 639 193 3362
Fragments arranged by size:
3362 639 193 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 296 1625 296
Size: 1329 3053
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DraIII CAC_nnn'GTG
Cuts at: 852 1161 852
Size: 309 4073
DrdI GACnn_nn'nnGTC
Cuts at: 2088 3432 2088
Size: 1344 3038
EaeI y'GGCC_r
Cuts at: 365 402 438 671 1100 1967 2141 2532 2559
Size: 37 36 233 429 867 174 391 27
Cuts at: 2559 3904 365
Size: 1345 843
Fragments arranged by size:
1345 867 843 429 391 233 174 37
36 27
EagI C'GGCC_G
Cuts at: 438 671 1967 438
Size: 233 1296 2853
Fragments arranged by size:
2853 1296 233
EarI CTCTTCn'nnn_
Cuts at: 175 766 1240 2405 2615 175
Size: 591 474 1165 210 1942
Fragments arranged by size:
1942 1165 591 474 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 3332 3478 4192 2590
Size: 742 146 714 2780
Fragments arranged by size:
2780 742 714 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 913 937 2207 2639 2992 3654 847
Size: 66 24 1270 432 353 662 1575
Fragments arranged by size:
1575 1270 662 432 353 66 24
EcoNI CCTnn'n_nnAGG
Cuts at: 484 484
Size: 4382
EcoO109I rG'GnC_Cy
Cuts at: 503 897 1521 503
Size: 394 624 3364
Fragments arranged by size:
3364 624 394
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 444 2401 444
Size: 1957 2425
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1325 1816 2064 3294 4065 1325
Size: 491 248 1230 771 1642
Fragments arranged by size:
1642 1230 771 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2224 2254 2256 2286 2224
Size: 30 2 30 4320
Fragments arranged by size:
4320 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
KasI G'GCGC_C
Cuts at: 1321 1812 2060 1321
Size: 491 248 3643
Fragments arranged by size:
3643 491 248
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3141 3325 3141
Size: 184 4198
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 589 2371 2498 589
Size: 1782 127 2473
Fragments arranged by size:
2473 1782 127
MspA1I CmG'CkG
Cuts at: 216 330 969 1209 1260 1493 1807 2167 2951
Size: 114 639 240 51 233 314 360 784
Cuts at: 2951 3196 216
Size: 245 1402
Fragments arranged by size:
1402 784 639 360 314 245 240 233
114 51
NaeI GCC'GGC
Cuts at: 225 675 1279 2563 225
Size: 450 604 1284 2044
Fragments arranged by size:
2044 1284 604 450
NarI GG'CG_CC
Cuts at: 1322 1813 2061 1322
Size: 491 248 3643
Fragments arranged by size:
3643 491 248
NcoI C'CATG_G
Cuts at: 140 140
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 223 673 1277 2561 223
Size: 450 604 1284 2044
Fragments arranged by size:
2044 1284 604 450
NruI TCG'CGA
Cuts at: 1361 1361
Size: 4382
NspI r_CATG'y
Cuts at: 223 534 1014 1131 1287 1435 1935 3538 223
Size: 311 480 117 156 148 500 1603 1067
Fragments arranged by size:
1603 1067 500 480 311 156 148 117
PasI CC'CwG_GG
Cuts at: 131 131
Size: 4382
PciI A'CATG_T
Cuts at: 3534 3534
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 1004 1052 1161 1331 1346 1004
Size: 48 109 170 15 4040
Fragments arranged by size:
4040 170 109 48 15
PfoI T'CCnGG_A
Cuts at: 824 1178 824
Size: 354 4028
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PmlI CAC'GTG
Cuts at: 659 659
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 4350
PpuMI rG'GwC_Cy
Cuts at: 897 897
Size: 4382
PspXI vC'TCGA_Gb
Cuts at: 277 277
Size: 4382
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 1807 2167 1807
Size: 360 4022
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SacII CC_GC'GG
Cuts at: 331 970 331
Size: 639 3743
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 175 766 1240 2405 2615 175
Size: 591 474 1165 210 1942
Fragments arranged by size:
1942 1165 591 474 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 868 3899 868
Size: 3031 1351
SexAI A'CCwGG_T
Cuts at: 848 848
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3078 3269 33
Size: 1392 685 968 191 1146
Fragments arranged by size:
1392 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1323 1814 2062 1323
Size: 491 248 3643
Fragments arranged by size:
3643 491 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 501 1404 501
Size: 903 3479
SmlI C'TyrA_G
Cuts at: 277 418 1240 1537 2452 3166 3428 277
Size: 141 822 297 915 714 262 1231
Fragments arranged by size:
1231 915 822 714 297 262 141
SpeI A'CTAG_T
Cuts at: 67 175 67
Size: 108 4274
SphI G_CATG'C
Cuts at: 223 1131 1287 1435 1935 223
Size: 908 156 148 500 2670
Fragments arranged by size:
2670 908 500 156 148
StuI AGG'CCT
Cuts at: 1069 1069
Size: 4382
StyI C'CwwG_G
Cuts at: 140 1516 1870 140
Size: 1376 354 2652
Fragments arranged by size:
2652 1376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 1965 2098 2557 2766 703
Size: 1262 133 459 209 2319
Fragments arranged by size:
2319 1262 459 209 133
TatI w'GTAC_w
Cuts at: 194 866 3897 194
Size: 672 3031 679
Fragments arranged by size:
3031 679 672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 889 921 3899 3931 889
Size: 32 2978 32 1340
Fragments arranged by size:
2978 1340 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1224 1877 1224
Size: 653 3729
XhoI C'TCGA_G
Cuts at: 277 277
Size: 4382
XmaI C'CCGG_G
Cuts at: 499 1402 499
Size: 903 3479
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AvrII BaeI BbvCI BfrBI BmtI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BsrGI BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PshAI PsiI PspOMI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI