Restriction map for
pF1KB5298
(Circular) (Six-base) MAPSORT of: pF1KB5298.seq Check: 6670 from: 1 to: 5015
pF1KB5298 5015 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 774 1468 774
Size: 694 4321
AccI GT'mk_AC
Cuts at: 572 695 1160 2053 572
Size: 123 465 893 3534
Fragments arranged by size:
3534 893 465 123
Acc65I G'GTAC_C
Cuts at: 2031 2031
Size: 5015
AclI AA'CG_TT
Cuts at: 4855 4855
Size: 5015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 481 2840 3272 3625 4287 315
Size: 166 2359 432 353 662 1043
Fragments arranged by size:
2359 1043 662 432 353 166
AfeI AGC'GCT
Cuts at: 1360 1360
Size: 5015
AflIII A'CryG_T
Cuts at: 2367 4167 4448 2367
Size: 1800 281 2934
Fragments arranged by size:
2934 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1118 1159 1744 1118
Size: 41 585 4389
Fragments arranged by size:
4389 585 41
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 109 141 109
Size: 32 4983
AlwNI CAG_nnn'CTG
Cuts at: 213 876 1260 2320 3758 213
Size: 663 384 1060 1438 1470
Fragments arranged by size:
1470 1438 1060 663 384
ApaI G_GGCC'C
Cuts at: 759 1838 759
Size: 1079 3936
ApaLI G'TGCA_C
Cuts at: 1639 1795 3853 1639
Size: 156 2058 2801
Fragments arranged by size:
2801 2058 156
ApoI r'AATT_y
Cuts at: 2019 4368 4624 2019
Size: 2349 256 2410
Fragments arranged by size:
2410 2349 256
AscI GG'CGCG_CC
Cuts at: 1554 1554
Size: 5015
AseI AT'TA_AT
Cuts at: 20 20
Size: 5015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
AvaI C'yCGr_G
Cuts at: 2035 2035
Size: 5015
BamHI G'GATC_C
Cuts at: 2040 2040
Size: 5015
BanI G'GyrC_C
Cuts at: 2031 2445 2693 2728 2031
Size: 414 248 35 4318
Fragments arranged by size:
4318 414 248 35
BanII G_rGCy'C
Cuts at: 452 759 818 973 1838 2029 3059 452
Size: 307 59 155 865 191 1030 2408
Fragments arranged by size:
2408 1030 865 307 191 155 59
BbeI G_GCGC'C
Cuts at: 2449 2697 2449
Size: 248 4767
BbvCI CC'TCA_GC
Cuts at: 706 860 1048 706
Size: 154 188 4673
Fragments arranged by size:
4673 188 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 270 304 270
Size: 34 4981
BciVI GTATCCnnnnn_n'
Cuts at: 1618 2913 3969 5015 1618
Size: 1295 1056 1046 1618
Fragments arranged by size:
1618 1295 1056 1046
BclI T'GATC_A
Cuts at: 2535 2535
Size: 5015
BglI GCCn_nnn'nGGC
Cuts at: 1111 1551 1787 1111
Size: 440 236 4339
Fragments arranged by size:
4339 440 236
BglII A'GATC_T
Cuts at: 800 2530 4567 800
Size: 1730 2037 1248
Fragments arranged by size:
2037 1730 1248
BlpI GC'TnA_GC
Cuts at: 451 2127 451
Size: 1676 3339
Bme1580I G_kGCm'C
Cuts at: 759 1643 1709 1799 1838 2640 2733 3857 759
Size: 884 66 90 39 802 93 1124 1917
Fragments arranged by size:
1917 1124 884 802 93 90 66 39
BmgBI CAC'GTC
Cuts at: 1638 1667 1638
Size: 29 4986
BmrI ACTGGGnnnn_n'
Cuts at: 1090 1989 2227 2308 2643 4787 1090
Size: 899 238 81 335 2144 1318
Fragments arranged by size:
2144 1318 899 335 238 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3382 3382
Size: 5015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 742 799 1236 1459 1983 466
Size: 276 57 437 223 524 3498
Fragments arranged by size:
3498 524 437 276 223 57
Bpu10I CC'TnA_GC
Cuts at: 457 706 860 1048 457
Size: 249 154 188 4424
Fragments arranged by size:
4424 249 188 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 618 850 1387 1924 2191 3070 3784 4082 618
Size: 232 537 537 267 879 714 298 1551
Fragments arranged by size:
1551 879 714 537 537 298 267 232
BsaI GGTCTCn'nnnn_
Cuts at: 730 732 4719 730
Size: 2 3987 1026
Fragments arranged by size:
3987 1026 2
BsaAI yAC'GTr
Cuts at: 1392 1794 2368 1392
Size: 402 574 4039
Fragments arranged by size:
4039 574 402
BsaBI GATnn'nnATC
Cuts at: 6 1453 2529 4566 6
Size: 1447 1076 2037 455
Fragments arranged by size:
2037 1447 1076 455
BsaHI Gr'CG_yC
Cuts at: 2446 2694 3396 4892 2446
Size: 248 702 1496 2569
Fragments arranged by size:
2569 1496 702 248
BsaWI w'CCGG_w
Cuts at: 938 2204 2725 3403 3814 3961 4235 938
Size: 1266 521 678 411 147 274 1718
Fragments arranged by size:
1718 1266 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 973 1003 109
Size: 30 834 30 4121
Fragments arranged by size:
4121 834 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 694 1570 1999 130
Size: 564 876 429 3146
Fragments arranged by size:
3146 876 564 429
BseYI C'CCAG_C
Cuts at: 131 346 895 958 1702 1823 1838 2440 3198
Size: 215 549 63 744 121 15 602 758
Cuts at: 3198 3863 4271 131
Size: 665 408 875
Fragments arranged by size:
875 758 744 665 602 549 408 215
121 63 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 793 808 1647 134
Size: 659 15 839 3502
Fragments arranged by size:
3502 839 659 15
BsiEI CG_ry'CG
Cuts at: 104 991 1467 2557 2603 3833 104
Size: 887 476 1090 46 1230 1286
Fragments arranged by size:
1286 1230 1090 887 476 46
BsiHKAI G_wGCw'C
Cuts at: 452 1148 1274 1643 1799 1880 2029 2807 2997
Size: 696 126 369 156 81 149 778 190
Cuts at: 2997 3857 452
Size: 860 1610
Fragments arranged by size:
1610 860 778 696 369 190 156 149
126 81
BsmI GAATG_Cn'
Cuts at: 1022 1353 1022
Size: 331 4684
Bsp1286I G_dGCh'C
Cuts at: 452 759 818 973 1148 1274 1643 1709 1799
Size: 307 59 155 175 126 369 66 90
Cuts at: 1799 1838 1880 2029 2640 2733 2807 2997 3059
Size: 39 42 149 611 93 74 190 62
Cuts at: 3059 3857 452
Size: 798 1610
Fragments arranged by size:
1610 798 611 369 307 190 175 155
149 126 93 90 74 66 62 59
42 39
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 774 1292 1468 2065 2279 2581 2962 774
Size: 518 176 597 214 302 381 2827
Fragments arranged by size:
2827 597 518 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 1763 1892 3307 3361 4845 5010 50
Size: 1713 129 1415 54 1484 165 55
Fragments arranged by size:
1713 1484 1415 165 129 55 54
BsrDI GCAATG_nn'
Cuts at: 468 1721 2927 468
Size: 1253 1206 2556
Fragments arranged by size:
2556 1253 1206
BsrFI r'CCGG_y
Cuts at: 575 3013 3194 575
Size: 2438 181 2396
Fragments arranged by size:
2438 2396 181
BsrGI T'GTAC_A
Cuts at: 465 465
Size: 5015
BssHII G'CGCG_C
Cuts at: 1554 1554
Size: 5015
BssSI C'ACGA_G
Cuts at: 811 3286 3994 811
Size: 2475 708 1832
Fragments arranged by size:
2475 1832 708
BstAPI GCAn_nnn'nTGC
Cuts at: 876 2392 876
Size: 1516 3499
BstBI TT'CG_AA
Cuts at: 3376 3376
Size: 5015
BstYI r'GATC_y
Cuts at: 1 500 800 1250 1742 2040 2507 2530 2865
Size: 499 300 450 492 298 467 23 335
Cuts at: 2865 3111 3417 3429 3515 4567 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 499 492 467 450 449 335 306
300 298 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1161 1161
Size: 5015
BtgI C'CryG_G
Cuts at: 107 387 1228 1773 1914 107
Size: 280 841 545 141 3208
Fragments arranged by size:
3208 841 545 280 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 283 1122 2965 3146 3309 283
Size: 839 1843 181 163 1989
Fragments arranged by size:
1989 1843 839 181 163
BtsI GCAGTG_nn'
Cuts at: 168 1100 1497 2258 168
Size: 932 397 761 2925
Fragments arranged by size:
2925 932 761 397
DraI TTT'AAA
Cuts at: 2014 2014
Size: 5015
DraIII CAC_nnn'GTG
Cuts at: 174 393 1647 174
Size: 219 1254 3542
Fragments arranged by size:
3542 1254 219
DrdI GACnn_nn'nnGTC
Cuts at: 1183 2721 4065 1183
Size: 1538 1344 2133
Fragments arranged by size:
2133 1538 1344
EaeI y'GGCC_r
Cuts at: 917 1464 1758 2600 2774 3165 3192 4537 917
Size: 547 294 842 174 391 27 1345 1395
Fragments arranged by size:
1395 1345 842 547 391 294 174 27
EagI C'GGCC_G
Cuts at: 1464 2600 1464
Size: 1136 3879
EarI CTCTTCn'nnn_
Cuts at: 259 368 548 3038 3248 259
Size: 109 180 2490 210 2026
Fragments arranged by size:
2490 2026 210 180 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3223 3965 4111 4825 3223
Size: 742 146 714 3413
Fragments arranged by size:
3413 742 714 146
EcoICRI GAG'CTC
Cuts at: 450 2027 450
Size: 1577 3438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 466 481 742 799 1236 1459 1983 2840
Size: 151 15 261 57 437 223 524 857
Cuts at: 2840 3272 3625 4287 315
Size: 432 353 662 1043
Fragments arranged by size:
1043 857 662 524 437 432 353 261
223 151 57 15
EcoNI CCTnn'n_nnAGG
Cuts at: 781 1264 781
Size: 483 4532
EcoO109I rG'GnC_Cy
Cuts at: 378 755 756 864 1834 1835 1919 2154 378
Size: 377 1 108 970 1 84 235 3239
Fragments arranged by size:
3239 970 377 235 108 84 1 1
EcoRI G'AATT_C
Cuts at: 2019 2019
Size: 5015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 5015
FspI TGC'GCA
Cuts at: 2796 2796
Size: 5015
HaeII r_GCGC'y
Cuts at: 926 1362 2449 2697 3927 4698 926
Size: 436 1087 248 1230 771 1243
Fragments arranged by size:
1243 1230 1087 771 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 973 1005 1939 1971 2857 2887 2889
Size: 32 154 32 934 32 886 30 2
Cuts at: 2889 2919 787
Size: 30 2883
Fragments arranged by size:
2883 934 886 154 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2054 2054
Size: 5015
KasI G'GCGC_C
Cuts at: 2445 2693 2445
Size: 248 4767
KpnI G_GTAC'C
Cuts at: 2035 2035
Size: 5015
MluI A'CGCG_T
Cuts at: 4448 4448
Size: 5015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 1151 3774 3958 971
Size: 180 2623 184 2028
Fragments arranged by size:
2623 2028 184 180
MscI TGG'CCA
Cuts at: 919 2776 919
Size: 1857 3158
MslI CAynn'nnrTG
Cuts at: 1141 1726 3004 3131 1141
Size: 585 1278 127 3025
Fragments arranged by size:
3025 1278 585 127
MspA1I CmG'CkG
Cuts at: 245 849 873 1241 1661 1775 1842 1916 2126
Size: 604 24 368 420 114 67 74 210
Cuts at: 2126 2440 2800 3584 3829 245
Size: 314 360 784 245 1431
Fragments arranged by size:
1431 784 604 420 368 360 314 245
210 114 74 67 24
NaeI GCC'GGC
Cuts at: 3196 3196
Size: 5015
NarI GG'CG_CC
Cuts at: 2446 2694 2446
Size: 248 4767
NcoI C'CATG_G
Cuts at: 107 107
Size: 5015
NgoMIV G'CCGG_C
Cuts at: 3194 3194
Size: 5015
NotI GC'GGCC_GC
Cuts at: 1464 1464
Size: 5015
NspI r_CATG'y
Cuts at: 1125 2068 2568 4171 1125
Size: 943 500 1603 1969
Fragments arranged by size:
1969 1603 943 500
PciI A'CATG_T
Cuts at: 4167 4167
Size: 5015
PflMI CCAn_nnn'nTGG
Cuts at: 1382 1382
Size: 5015
PfoI T'CCnGG_A
Cuts at: 236 236
Size: 5015
PmeI GTTT'AAAC
Cuts at: 2014 2014
Size: 5015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 109 141 3461 3493 109
Size: 32 3320 32 1631
Fragments arranged by size:
3320 1631 32 32
PpuMI rG'GwC_Cy
Cuts at: 378 864 1919 378
Size: 486 1055 3474
Fragments arranged by size:
3474 1055 486
PshAI GACnn'nnGTC
Cuts at: 1677 1677
Size: 5015
PspOMI G'GGCC_C
Cuts at: 755 1834 755
Size: 1079 3936
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 4983
PstI C_TGCA'G
Cuts at: 872 968 1265 2062 2747 872
Size: 96 297 797 685 3140
Fragments arranged by size:
3140 797 685 297 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5015
PvuII CAG'CTG
Cuts at: 1661 1842 2440 2800 1661
Size: 181 598 360 3876
Fragments arranged by size:
3876 598 360 181
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5015
SacI G_AGCT'C
Cuts at: 452 2029 452
Size: 1577 3438
SacII CC_GC'GG
Cuts at: 1776 1917 1776
Size: 141 4874
SalI G'TCGA_C
Cuts at: 2052 2052
Size: 5015
SanDI GG'GwC_CC
Cuts at: 1919 1919
Size: 5015
SapI GCTCTTCn'nnn_
Cuts at: 3038 3248 3038
Size: 210 4805
SbfI CC_TGCA'GG
Cuts at: 2062 2062
Size: 5015
ScaI AGT'ACT
Cuts at: 4532 4532
Size: 5015
SexAI A'CCwGG_T
Cuts at: 1643 1643
Size: 5015
SfcI C'TryA_G
Cuts at: 33 868 952 964 1261 1656 2058 2743 3711
Size: 835 84 12 297 395 402 685 968
Cuts at: 3711 3902 33
Size: 191 1146
Fragments arranged by size:
1146 968 835 685 402 395 297 191
84 12
SfoI GGC'GCC
Cuts at: 2447 2695 2447
Size: 248 4767
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
SmaI CCC'GGG
Cuts at: 2037 2037
Size: 5015
SmlI C'TyrA_G
Cuts at: 597 829 1402 1903 2170 3085 3799 4061 597
Size: 232 573 501 267 915 714 262 1551
Fragments arranged by size:
1551 915 714 573 501 267 262 232
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5015
SphI G_CATG'C
Cuts at: 2068 2568 2068
Size: 500 4515
StuI AGG'CCT
Cuts at: 1862 1862
Size: 5015
StyI C'CwwG_G
Cuts at: 107 1188 2149 2503 107
Size: 1081 961 354 2619
Fragments arranged by size:
2619 1081 961 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 145 179 534 754 2598 2731 3190 3399 145
Size: 34 355 220 1844 133 459 209 1761
Fragments arranged by size:
1844 1761 459 355 220 209 133 34
TatI w'GTAC_w
Cuts at: 465 4530 465
Size: 4065 950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 985 1017 4532 4564 379
Size: 32 574 32 3515 32 830
Fragments arranged by size:
3515 830 574 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2812 2812
Size: 5015
XbaI T'CTAG_A
Cuts at: 2046 2046
Size: 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1860 2510 1860
Size: 650 4365
XmaI C'CCGG_G
Cuts at: 2035 2035
Size: 5015
XmnI GAAnn'nnTTC
Cuts at: 1096 1096
Size: 5015
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AvrII BaeI BbsI
BfrBI BmtI BsiWI BsmBI BspEI BstEII BstXI Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PmlI PsiI
PspXI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI