Restriction map for
pF1KB5297
(Circular) (Six-base) MAPSORT of: pF1KB5297.seq Check: 7434 from: 1 to: 4013
pF1KB5297 4013 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 383 1051 383
Size: 668 3345
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1838 2270 3017 3285 1838
Size: 432 747 268 2566
Fragments arranged by size:
2566 747 432 268
AflIII A'CryG_T
Cuts at: 633 748 1365 2469 3446 633
Size: 115 617 1104 977 1200
Fragments arranged by size:
1200 1104 977 617 115
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 238 1318 2885 238
Size: 1080 1567 1366
Fragments arranged by size:
1567 1366 1080
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 112 271 1017 3366 3622 112
Size: 159 746 2349 256 503
Fragments arranged by size:
2349 746 503 256 159
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 1033 1033
Size: 4013
BamHI G'GATC_C
Cuts at: 920 1038 920
Size: 118 3895
BanI G'GyrC_C
Cuts at: 358 1029 1443 1691 1726 358
Size: 671 414 248 35 2645
Fragments arranged by size:
2645 671 414 248 35
BanII G_rGCy'C
Cuts at: 1027 2057 1027
Size: 1030 2983
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4013
BfrBI ATG'CAT
Cuts at: 849 849
Size: 4013
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 1638 1731 2787 1638
Size: 93 1056 2864
Fragments arranged by size:
2864 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 364 1225 1306 1641 3785 364
Size: 861 81 335 2144 592
Fragments arranged by size:
2144 861 592 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 755 346
Size: 409 3604
Bpu10I CC'TnA_GC
Cuts at: 929 929
Size: 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3717 3717
Size: 4013
BsaAI yAC'GTr
Cuts at: 1366 1366
Size: 4013
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2401 2675 2822 3233 1202
Size: 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 210
Size: 4013
BseYI C'CCAG_C
Cuts at: 238 1438 2196 2773 3269 238
Size: 1200 758 577 496 982
Fragments arranged by size:
1200 982 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2787 1027
Size: 778 190 792 2253
Fragments arranged by size:
2253 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1027 1638 1731 1805 1995 2057 2787 1027
Size: 611 93 74 190 62 730 2253
Fragments arranged by size:
2253 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1063 1277 1579 1960 1063
Size: 214 302 381 3116
Fragments arranged by size:
3116 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3843 4008 50
Size: 2255 54 1484 165 55
Fragments arranged by size:
2255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 587 1925 587
Size: 1338 2675
BsrFI r'CCGG_y
Cuts at: 211 2011 2192 2401 211
Size: 1800 181 209 1823
Fragments arranged by size:
1823 1800 209 181
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstYI r'GATC_y
Cuts at: 1 507 920 1038 1505 1528 1863 2109 3121
Size: 506 413 118 467 23 335 246 1012
Cuts at: 3121 3207 3219 3565 1
Size: 86 12 346 449
Fragments arranged by size:
1012 506 467 449 413 346 335 246
118 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 824 824
Size: 4013
BtgI C'CryG_G
Cuts at: 597 597
Size: 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 1256 1256
Size: 4013
DraI TTT'AAA
Cuts at: 950 1012 950
Size: 62 3951
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 600 1598 1772 2163 2190 3535 600
Size: 998 174 391 27 1345 1078
Fragments arranged by size:
1345 1078 998 391 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 755 1838 2270 3017 3285 346
Size: 409 1083 432 747 268 1074
Fragments arranged by size:
1083 1074 747 432 409 268
EcoO109I rG'GnC_Cy
Cuts at: 808 1152 808
Size: 344 3669
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1855 1885 1887 1917 1855
Size: 30 2 30 3951
Fragments arranged by size:
3951 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
HindIII A'AGCT_T
Cuts at: 178 178
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 666 2684 2868 666
Size: 2018 184 1811
Fragments arranged by size:
2018 1811 184
MscI TGG'CCA
Cuts at: 602 1774 602
Size: 1172 2841
MslI CAynn'nnrTG
Cuts at: 753 2002 2129 753
Size: 1249 127 2637
Fragments arranged by size:
2637 1249 127
MspA1I CmG'CkG
Cuts at: 337 628 1124 1438 1798 2811 3056 337
Size: 291 496 314 360 1013 245 1294
Fragments arranged by size:
1294 1013 496 360 314 291 245
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4013
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NcoI C'CATG_G
Cuts at: 597 597
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4013
NsiI A_TGCA'T
Cuts at: 851 851
Size: 4013
NspI r_CATG'y
Cuts at: 637 752 1066 1566 2473 637
Size: 115 314 500 907 2177
Fragments arranged by size:
2177 907 500 314 115
PciI A'CATG_T
Cuts at: 633 748 2469 633
Size: 115 1721 2177
Fragments arranged by size:
2177 1721 115
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PpuMI rG'GwC_Cy
Cuts at: 808 808
Size: 4013
PstI C_TGCA'G
Cuts at: 391 1060 1745 391
Size: 669 685 2659
Fragments arranged by size:
2659 685 669
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 337 628 1438 1798 337
Size: 291 810 360 2552
Fragments arranged by size:
2552 810 360 291
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SanDI GG'GwC_CC
Cuts at: 808 808
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 387 893 1056 1741 2734 2925 33
Size: 354 506 163 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 354 191 163
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SgrAI Cr'CCGG_yG
Cuts at: 211 211
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 1168 2083 2575 2837 1168
Size: 915 492 262 2344
Fragments arranged by size:
2344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 362 597 1147 1501 362
Size: 235 550 354 2874
Fragments arranged by size:
2874 550 354 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 630 1596 1729 2188 2397 346
Size: 284 966 133 459 209 1962
Fragments arranged by size:
1962 966 459 284 209 133
TatI w'GTAC_w
Cuts at: 540 796 3528 540
Size: 256 2732 1025
Fragments arranged by size:
2732 1025 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 3981
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 1508
Size: 4013
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI