Restriction map for
pF1KB5294
(Circular) (Six-base) MAPSORT of: pF1KB5294.seq Check: 1055 from: 1 to: 4142
pF1KB5294 4142 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 585 585
Size: 4142
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 553 3982 553
Size: 3429 713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1967 2399 3146 3414 520
Size: 1447 432 747 268 1248
Fragments arranged by size:
1447 1248 747 432 268
AflIII A'CryG_T
Cuts at: 989 1494 2598 3575 989
Size: 505 1104 977 1556
Fragments arranged by size:
1556 1104 977 505
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 553 585 553
Size: 32 4110
AlwNI CAG_nnn'CTG
Cuts at: 1447 3014 1447
Size: 1567 2575
ApaLI G'TGCA_C
Cuts at: 2912 2912
Size: 4142
ApoI r'AATT_y
Cuts at: 1146 3495 3751 1146
Size: 2349 256 1537
Fragments arranged by size:
2349 1537 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 492 1162 492
Size: 670 3472
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 1158 1572 1820 1855 1158
Size: 414 248 35 3445
Fragments arranged by size:
3445 414 248 35
BanII G_rGCy'C
Cuts at: 821 1156 2186 821
Size: 335 1030 2777
Fragments arranged by size:
2777 1030 335
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 813 847 90
Size: 34 689 34 3385
Fragments arranged by size:
3385 689 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglI GCCn_nnn'nGGC
Cuts at: 112 253 715 112
Size: 141 462 3539
Fragments arranged by size:
3539 462 141
BglII A'GATC_T
Cuts at: 1657 3694 1657
Size: 2037 2105
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 622 1767 1860 2916 622
Size: 1145 93 1056 1848
Fragments arranged by size:
1848 1145 1056 93
BmgBI CAC'GTC
Cuts at: 992 992
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 1354 1435 1770 3914 1354
Size: 81 335 2144 1582
Fragments arranged by size:
2144 1582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 2509 777
Size: 1732 2410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 601 1127 111
Size: 490 526 3126
Fragments arranged by size:
3126 526 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2197 2689 2987 1318
Size: 879 492 298 2473
Fragments arranged by size:
2473 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 577 955 3846 577
Size: 378 2891 873
Fragments arranged by size:
2891 873 378
BsaAI yAC'GTr
Cuts at: 1495 1495
Size: 4142
BsaBI GATnn'nnATC
Cuts at: 6 1129 1656 3693 6
Size: 1123 527 2037 455
Fragments arranged by size:
2037 1123 527 455
BsaHI Gr'CG_yC
Cuts at: 192 1573 1821 2523 4019 192
Size: 1381 248 702 1496 315
Fragments arranged by size:
1496 1381 702 315 248
BsaWI w'CCGG_w
Cuts at: 1331 1852 2530 2804 2951 3362 1331
Size: 521 678 274 147 411 2111
Fragments arranged by size:
2111 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 553
Size: 30 4112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 907 907
Size: 4142
BseYI C'CCAG_C
Cuts at: 696 1567 2325 2902 3398 696
Size: 871 758 577 496 1440
Fragments arranged by size:
1440 871 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2938 104
Size: 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1156 1934 2124 2916 1156
Size: 778 190 792 2382
Fragments arranged by size:
2382 792 778 190
BsmI GAATG_Cn'
Cuts at: 333 863 1088 333
Size: 530 225 3387
Fragments arranged by size:
3387 530 225
Bsp1286I G_dGCh'C
Cuts at: 622 821 1156 1767 1860 1934 2124 2186 2916
Size: 199 335 611 93 74 190 62 730
Cuts at: 2916 622
Size: 1848
Fragments arranged by size:
1848 730 611 335 199 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 1091 3318 1091
Size: 2227 1915
BspMI ACCTGCnnnn'nnnn_
Cuts at: 585 1192 1406 1708 2089 585
Size: 607 214 302 381 2638
Fragments arranged by size:
2638 607 381 302 214
BsrBI CCG'CTC
Cuts at: 50 491 722 2434 2488 3972 4137 50
Size: 441 231 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 441 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 261 541 2140 2321 2530 261
Size: 280 1599 181 209 1873
Fragments arranged by size:
1873 1599 280 209 181
BssSI C'ACGA_G
Cuts at: 2413 2771 2413
Size: 358 3784
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1519
Size: 4142
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstXI CCAn_nnnn'nTGG
Cuts at: 1105 1105
Size: 4142
BstYI r'GATC_y
Cuts at: 1 1167 1634 1657 1992 2238 3250 3336 3348
Size: 1166 467 23 335 246 1012 86 12
Cuts at: 3348 3694 1
Size: 346 449
Fragments arranged by size:
1166 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 902 902
Size: 4142
BtgI C'CryG_G
Cuts at: 1058 1058
Size: 4142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 816 858 2092 2273 2436 816
Size: 42 1234 181 163 2522
Fragments arranged by size:
2522 1234 181 163 42
BtsI GCAGTG_nn'
Cuts at: 981 1385 981
Size: 404 3738
ClaI AT'CG_AT
Cuts at: 1004 1004
Size: 4142
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 113 231 320 707 1727 1901 2292 2319 3664
Size: 118 89 387 1020 174 391 27 1345
Cuts at: 3664 113
Size: 591
Fragments arranged by size:
1345 1020 591 391 387 174 118 89
27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 455 2165 2375 455
Size: 1710 210 2222
Fragments arranged by size:
2222 1710 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 271 2350 2660 2806 3952 271
Size: 2079 310 146 1146 461
Fragments arranged by size:
2079 1146 461 310 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 520 601 1127 1967 2399 3146 3414 111
Size: 409 81 526 840 432 747 268 839
Fragments arranged by size:
840 839 747 526 432 409 268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 512 512
Size: 4142
EcoO109I rG'GnC_Cy
Cuts at: 504 525 1281 504
Size: 21 756 3365
Fragments arranged by size:
3365 756 21
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 364 628 951 2161 364
Size: 264 323 1210 2345
Fragments arranged by size:
2345 1210 323 264
FspI TGC'GCA
Cuts at: 913 1923 913
Size: 1010 3132
HaeII r_GCGC'y
Cuts at: 1576 1824 2846 3825 1576
Size: 248 1022 979 1893
Fragments arranged by size:
1893 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 219 251 1984 2014 2016 2046 219
Size: 32 1733 30 2 30 2315
Fragments arranged by size:
2315 1733 32 30 30 2
HincII GTy'rAC
Cuts at: 534 1181 534
Size: 647 3495
HpaI GTT'AAC
Cuts at: 534 534
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 217 1142 2813 2997 217
Size: 925 1671 184 1362
Fragments arranged by size:
1671 1362 925 184
MscI TGG'CCA
Cuts at: 115 322 709 1903 115
Size: 207 387 1194 2354
Fragments arranged by size:
2354 1194 387 207
MslI CAynn'nnrTG
Cuts at: 2131 2258 2131
Size: 127 4015
MspA1I CmG'CkG
Cuts at: 1253 1567 1927 2940 3185 1253
Size: 314 360 1013 245 2210
Fragments arranged by size:
2210 1013 360 314 245
NaeI GCC'GGC
Cuts at: 543 2323 543
Size: 1780 2362
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NcoI C'CATG_G
Cuts at: 1058 1058
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 541 2321 541
Size: 1780 2362
NspI r_CATG'y
Cuts at: 522 696 1195 1695 2602 522
Size: 174 499 500 907 2062
Fragments arranged by size:
2062 907 500 499 174
PasI CC'CwG_GG
Cuts at: 792 792
Size: 4142
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PflMI CCAn_nnn'nTGG
Cuts at: 1059 1059
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 553 585 3281 3313 553
Size: 32 2696 32 1382
Fragments arranged by size:
2696 1382 32 32
PpuMI rG'GwC_Cy
Cuts at: 504 504
Size: 4142
PshAI GACnn'nnGTC
Cuts at: 135 135
Size: 4142
PspXI vC'TCGA_Gb
Cuts at: 492 492
Size: 4142
PstI C_TGCA'G
Cuts at: 454 973 1189 1874 454
Size: 519 216 685 2722
Fragments arranged by size:
2722 685 519 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 1567 1927 1567
Size: 360 3782
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 2165 2375 2165
Size: 210 3932
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 934 3659 934
Size: 2725 1417
SfcI C'TryA_G
Cuts at: 33 450 969 1185 1870 2863 3054 33
Size: 417 519 216 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 417 216 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 715 715
Size: 4142
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 492 1297 2212 2704 2966 492
Size: 805 915 492 262 1668
Fragments arranged by size:
1668 915 805 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 696 1195 1695 696
Size: 499 500 3143
Fragments arranged by size:
3143 500 499
StyI C'CwwG_G
Cuts at: 1058 1276 1630 1058
Size: 218 354 3570
Fragments arranged by size:
3570 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 437 1725 1858 2317 2526 437
Size: 1288 133 459 209 2053
Fragments arranged by size:
2053 1288 459 209 133
TatI w'GTAC_w
Cuts at: 932 3657 932
Size: 2725 1417
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4110
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1637 1637
Size: 4142
XhoI C'TCGA_G
Cuts at: 492 492
Size: 4142
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I CspCI DraIII
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PsiI PspOMI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI