Restriction map for
pF1KB5291
(Circular) (Six-base) MAPSORT of: pF1KB5291.seq Check: 5373 from: 1 to: 3620
pF1KB5291 3620 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 386 658 386
Size: 272 3348
Acc65I G'GTAC_C
Cuts at: 636 636
Size: 3620
AclI AA'CG_TT
Cuts at: 3460 3460
Size: 3620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1445 1877 2624 2892 619
Size: 826 432 747 268 1347
Fragments arranged by size:
1347 826 747 432 268
AflIII A'CryG_T
Cuts at: 972 2076 3053 972
Size: 1104 977 1539
Fragments arranged by size:
1539 1104 977
AgeI A'CCGG_T
Cuts at: 2008 2008
Size: 3620
AlwNI CAG_nnn'CTG
Cuts at: 122 925 2492 122
Size: 803 1567 1250
Fragments arranged by size:
1567 1250 803
ApaI G_GGCC'C
Cuts at: 314 314
Size: 3620
ApaLI G'TGCA_C
Cuts at: 2390 2390
Size: 3620
ApoI r'AATT_y
Cuts at: 137 624 2973 3229 137
Size: 487 2349 256 528
Fragments arranged by size:
2349 528 487 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
AvaI C'yCGr_G
Cuts at: 283 640 283
Size: 357 3263
BamHI G'GATC_C
Cuts at: 645 645
Size: 3620
BanI G'GyrC_C
Cuts at: 111 125 636 1050 1298 1333 111
Size: 14 511 414 248 35 2398
Fragments arranged by size:
2398 511 414 248 35 14
BanII G_rGCy'C
Cuts at: 314 634 1664 314
Size: 320 1030 2270
Fragments arranged by size:
2270 1030 320
BbeI G_GCGC'C
Cuts at: 1054 1302 1054
Size: 248 3372
BciVI GTATCCnnnnn_n'
Cuts at: 1518 2279 3620 1518
Size: 761 1341 1518
Fragments arranged by size:
1518 1341 761
BclI T'GATC_A
Cuts at: 1140 1140
Size: 3620
BglII A'GATC_T
Cuts at: 1135 3172 1135
Size: 2037 1583
BlpI GC'TnA_GC
Cuts at: 732 732
Size: 3620
Bme1580I G_kGCm'C
Cuts at: 130 314 1245 1338 2394 130
Size: 184 931 93 1056 1356
Fragments arranged by size:
1356 1056 931 184 93
BmrI ACTGGGnnnn_n'
Cuts at: 832 913 1248 3392 832
Size: 81 335 2144 1060
Fragments arranged by size:
2144 1060 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1675 2167 2465 796
Size: 879 492 298 1951
Fragments arranged by size:
1951 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3324 3324
Size: 3620
BsaAI yAC'GTr
Cuts at: 973 973
Size: 3620
BsaBI GATnn'nnATC
Cuts at: 6 1134 3171 6
Size: 1128 2037 455
Fragments arranged by size:
2037 1128 455
BsaHI Gr'CG_yC
Cuts at: 1051 1299 2001 3497 1051
Size: 248 702 1496 1174
Fragments arranged by size:
1496 1174 702 248
BsaWI w'CCGG_w
Cuts at: 809 1330 2008 2282 2429 2840 809
Size: 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 310
Size: 3620
BseYI C'CCAG_C
Cuts at: 129 1045 1803 2380 2876 129
Size: 916 758 577 496 873
Fragments arranged by size:
916 873 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 3620
BsiEI CG_ry'CG
Cuts at: 104 1162 1208 2416 104
Size: 1058 46 1208 1308
Fragments arranged by size:
1308 1208 1058 46
BsiHKAI G_wGCw'C
Cuts at: 634 1412 1602 2394 634
Size: 778 190 792 1860
Fragments arranged by size:
1860 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 130 314 634 1245 1338 1412 1602 1664 2394
Size: 184 320 611 93 74 190 62 730
Cuts at: 2394 130
Size: 1356
Fragments arranged by size:
1356 730 611 320 190 184 93 74
62
BspEI T'CCGG_A
Cuts at: 2840 2840
Size: 3620
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 361 670 884 1186 1567 361
Size: 309 214 302 381 2414
Fragments arranged by size:
2414 381 309 302 214
BsrBI CCG'CTC
Cuts at: 50 1912 1966 3450 3615 50
Size: 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 185 1532 185
Size: 1347 2273
BsrFI r'CCGG_y
Cuts at: 1618 1799 2008 1618
Size: 181 209 3230
Fragments arranged by size:
3230 209 181
BssSI C'ACGA_G
Cuts at: 1891 2249 1891
Size: 358 3262
BstAPI GCAn_nnn'nTGC
Cuts at: 997 997
Size: 3620
BstBI TT'CG_AA
Cuts at: 1981 1981
Size: 3620
BstXI CCAn_nnnn'nTGG
Cuts at: 321 360 321
Size: 39 3581
BstYI r'GATC_y
Cuts at: 1 253 645 1112 1135 1470 1716 2728 2814
Size: 252 392 467 23 335 246 1012 86
Cuts at: 2814 2826 3172 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 392 346 335 252 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 314 107
Size: 207 3413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1570 1751 1914 1570
Size: 181 163 3276
Fragments arranged by size:
3276 181 163
BtsI GCAGTG_nn'
Cuts at: 863 863
Size: 3620
DraI TTT'AAA
Cuts at: 619 619
Size: 3620
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2184 1326
Size: 858 2762
EaeI y'GGCC_r
Cuts at: 1205 1379 1770 1797 3142 1205
Size: 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1205 1205
Size: 3620
EarI CTCTTCn'nnn_
Cuts at: 180 1643 1853 180
Size: 1463 210 1947
Fragments arranged by size:
1947 1463 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1828 2138 2284 3430 1828
Size: 310 146 1146 2018
Fragments arranged by size:
2018 1146 310 146
EcoICRI GAG'CTC
Cuts at: 632 632
Size: 3620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 619 1445 1877 2624 2892 412
Size: 207 826 432 747 268 1140
Fragments arranged by size:
1140 826 747 432 268 207
EcoO109I rG'GnC_Cy
Cuts at: 310 759 310
Size: 449 3171
EcoRI G'AATT_C
Cuts at: 624 624
Size: 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 1639
Size: 3620
FspI TGC'GCA
Cuts at: 1401 1401
Size: 3620
HaeII r_GCGC'y
Cuts at: 1054 1302 2324 3303 1054
Size: 248 1022 979 1371
Fragments arranged by size:
1371 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 447 479 535 567 1462 1492 1494 1524 447
Size: 32 56 32 895 30 2 30 2543
Fragments arranged by size:
2543 895 56 32 32 30 30 2
HincII GTy'rAC
Cuts at: 387 659 387
Size: 272 3348
KasI G'GCGC_C
Cuts at: 1050 1298 1050
Size: 248 3372
KpnI G_GTAC'C
Cuts at: 640 640
Size: 3620
MluI A'CGCG_T
Cuts at: 3053 3053
Size: 3620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2475 2291
Size: 184 3436
MscI TGG'CCA
Cuts at: 1381 1381
Size: 3620
MslI CAynn'nnrTG
Cuts at: 217 319 1609 1736 217
Size: 102 1290 127 2101
Fragments arranged by size:
2101 1290 127 102
MspA1I CmG'CkG
Cuts at: 731 1045 1405 2418 2663 731
Size: 314 360 1013 245 1688
Fragments arranged by size:
1688 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1801 1801
Size: 3620
NarI GG'CG_CC
Cuts at: 1051 1299 1051
Size: 248 3372
NcoI C'CATG_G
Cuts at: 107 314 107
Size: 207 3413
NgoMIV G'CCGG_C
Cuts at: 1799 1799
Size: 3620
NspI r_CATG'y
Cuts at: 511 673 1173 2080 511
Size: 162 500 907 2051
Fragments arranged by size:
2051 907 500 162
PciI A'CATG_T
Cuts at: 2076 2076
Size: 3620
PflMI CCAn_nnn'nTGG
Cuts at: 122 122
Size: 3620
PfoI T'CCnGG_A
Cuts at: 546 546
Size: 3620
PmeI GTTT'AAAC
Cuts at: 619 619
Size: 3620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 3588
PsiI TTA'TAA
Cuts at: 577 577
Size: 3620
PspOMI G'GGCC_C
Cuts at: 310 310
Size: 3620
PstI C_TGCA'G
Cuts at: 667 1352 667
Size: 685 2935
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3620
PvuII CAG'CTG
Cuts at: 1045 1405 1045
Size: 360 3260
RsrII CG'GwC_CG
Cuts at: 1815 1815
Size: 3620
SacI G_AGCT'C
Cuts at: 634 634
Size: 3620
SalI G'TCGA_C
Cuts at: 385 657 385
Size: 272 3348
SapI GCTCTTCn'nnn_
Cuts at: 180 1643 1853 180
Size: 1463 210 1947
Fragments arranged by size:
1947 1463 210
SbfI CC_TGCA'GG
Cuts at: 667 667
Size: 3620
ScaI AGT'ACT
Cuts at: 3137 3137
Size: 3620
SexAI A'CCwGG_T
Cuts at: 121 121
Size: 3620
SfcI C'TryA_G
Cuts at: 33 663 1348 2341 2532 33
Size: 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191
SfoI GGC'GCC
Cuts at: 1052 1300 1052
Size: 248 3372
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
SmaI CCC'GGG
Cuts at: 642 642
Size: 3620
SmlI C'TyrA_G
Cuts at: 775 1690 2182 2444 775
Size: 915 492 262 1951
Fragments arranged by size:
1951 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3620
SphI G_CATG'C
Cuts at: 673 1173 673
Size: 500 3120
StyI C'CwwG_G
Cuts at: 107 314 754 1108 107
Size: 207 440 354 2619
Fragments arranged by size:
2619 440 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 1203 1336 1795 2004 129
Size: 1074 133 459 209 1745
Fragments arranged by size:
1745 1074 459 209 133
TatI w'GTAC_w
Cuts at: 3135 3135
Size: 3620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3588
Tth111I GACn'n_nGTC
Cuts at: 1417 1417
Size: 3620
XbaI T'CTAG_A
Cuts at: 336 651 336
Size: 315 3305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1115 1115
Size: 3620
XmaI C'CCGG_G
Cuts at: 640 640
Size: 3620
XmnI GAAnn'nnTTC
Cuts at: 537 537
Size: 3620
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI