Restriction map for
pF1KB5282
(Circular) (Six-base) MAPSORT of: pF1KB5282.seq Check: 1489 from: 1 to: 3788
pF1KB5282 3788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 1613 2045 2792 3060 708
Size: 905 432 747 268 1436
Fragments arranged by size:
1436 905 747 432 268
AflII C'TTAA_G
Cuts at: 373 373
Size: 3788
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 500
Size: 32 3756
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 186 346 390 792 3141 3397 186
Size: 160 44 402 2349 256 577
Fragments arranged by size:
2349 577 402 256 160 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 129 804 1218 1466 1501 129
Size: 675 414 248 35 2416
Fragments arranged by size:
2416 675 414 248 35
BanII G_rGCy'C
Cuts at: 802 1832 802
Size: 1030 2758
BbeI G_GCGC'C
Cuts at: 133 1222 1470 133
Size: 1089 248 2451
Fragments arranged by size:
2451 1089 248
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglII A'GATC_T
Cuts at: 729 1303 3340 729
Size: 574 2037 1177
Fragments arranged by size:
2037 1177 574
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 231 554 1000 1081 1416 3560 231
Size: 323 446 81 335 2144 459
Fragments arranged by size:
2144 459 446 335 323 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1843 2335 2633 964
Size: 879 492 298 2119
Fragments arranged by size:
2119 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 197 1141 197
Size: 944 2844
BsaBI GATnn'nnATC
Cuts at: 6 734 1302 3339 6
Size: 728 568 2037 455
Fragments arranged by size:
2037 728 568 455
BsaHI Gr'CG_yC
Cuts at: 130 149 1219 1467 2169 3665 130
Size: 19 1070 248 702 1496 253
Fragments arranged by size:
1496 1070 702 253 248 19
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 139
Size: 3788
BseYI C'CCAG_C
Cuts at: 1213 1971 2548 3044 1213
Size: 758 577 496 1957
Fragments arranged by size:
1957 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 761 802 1580 1770 2562 761
Size: 41 778 190 792 1987
Fragments arranged by size:
1987 792 778 190 41
Bsp1286I G_dGCh'C
Cuts at: 761 802 1413 1506 1580 1770 1832 2562 761
Size: 41 611 93 74 190 62 730 1987
Fragments arranged by size:
1987 730 611 190 93 74 62 41
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 270 2080 2134 3618 3783 50
Size: 220 1810 54 1484 165 55
Fragments arranged by size:
1810 1484 220 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 1786 1967 2176 1786
Size: 181 209 3398
Fragments arranged by size:
3398 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 3788
BstYI r'GATC_y
Cuts at: 1 729 813 1280 1303 1638 1884 2896 2982
Size: 728 84 467 23 335 246 1012 86
Cuts at: 2982 2994 3340 1
Size: 12 346 449
Fragments arranged by size:
1012 728 467 449 346 335 246 86
84 23 12
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 3437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 500 1738 1919 2082 500
Size: 1238 181 163 2206
Fragments arranged by size:
2206 1238 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
ClaI AT'CG_AT
Cuts at: 399 399
Size: 3788
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 316 1494 2352 316
Size: 1178 858 1752
Fragments arranged by size:
1752 1178 858
EaeI y'GGCC_r
Cuts at: 110 169 275 1373 1547 1938 1965 3310 110
Size: 59 106 1098 174 391 27 1345 588
Fragments arranged by size:
1345 1098 588 391 174 106 59 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 619 1811 2021 619
Size: 1192 210 2386
Fragments arranged by size:
2386 1192 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 1613 2045 2792 3060 708
Size: 905 432 747 268 1436
Fragments arranged by size:
1436 905 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 927 927
Size: 3788
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 133 1222 1470 2492 3471 133
Size: 1089 248 1022 979 450
Fragments arranged by size:
1089 1022 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1660 1662 1692 1630
Size: 30 2 30 3726
Fragments arranged by size:
3726 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 129 1218 1466 129
Size: 1089 248 2451
Fragments arranged by size:
2451 1089 248
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 519 2459 2643 519
Size: 1940 184 1664
Fragments arranged by size:
1940 1664 184
MscI TGG'CCA
Cuts at: 171 277 1549 171
Size: 106 1272 2410
Fragments arranged by size:
2410 1272 106
MslI CAynn'nnrTG
Cuts at: 212 1777 1904 212
Size: 1565 127 2096
Fragments arranged by size:
2096 1565 127
MspA1I CmG'CkG
Cuts at: 899 1213 1573 2586 2831 899
Size: 314 360 1013 245 1856
Fragments arranged by size:
1856 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 130 1219 1467 130
Size: 1089 248 2451
Fragments arranged by size:
2451 1089 248
NcoI C'CATG_G
Cuts at: 107 458 107
Size: 351 3437
NdeI CA'TA_TG
Cuts at: 742 742
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PflMI CCAn_nnn'nTGG
Cuts at: 459 459
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PstI C_TGCA'G
Cuts at: 835 1520 835
Size: 685 3103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 831 1516 2509 2700 33
Size: 798 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 191
SfoI GGC'GCC
Cuts at: 131 1220 1468 131
Size: 1089 248 2451
Fragments arranged by size:
2451 1089 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 373 943 1858 2350 2612 373
Size: 570 915 492 262 1549
Fragments arranged by size:
1549 915 570 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 107 278 458 922 1276 107
Size: 171 180 464 354 2619
Fragments arranged by size:
2619 464 354 180 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 451 1371 1504 1963 2172 451
Size: 920 133 459 209 2067
Fragments arranged by size:
2067 920 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
XmnI GAAnn'nnTTC
Cuts at: 190 190
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI