Restriction map for
pF1KB5281
(Circular) (Six-base) MAPSORT of: pF1KB5281.seq Check: 5415 from: 1 to: 3929
pF1KB5281 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 856 856
Size: 3929
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2186 2539 3201 1754
Size: 432 353 662 2482
Fragments arranged by size:
2482 662 432 353
AflIII A'CryG_T
Cuts at: 1281 3081 3362 1281
Size: 1800 281 1848
Fragments arranged by size:
1848 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 628 628
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 426 471 621 1234 2672 426
Size: 45 150 613 1438 1683
Fragments arranged by size:
1683 1438 613 150 45
ApaI G_GGCC'C
Cuts at: 362 653 362
Size: 291 3638
ApaLI G'TGCA_C
Cuts at: 2767 2767
Size: 3929
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AscI GG'CGCG_CC
Cuts at: 536 536
Size: 3929
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 148 190 355 361 378 949 148
Size: 42 165 6 17 571 3128
Fragments arranged by size:
3128 571 165 42 17 6
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 333 643 720 727 945 1359 1607 1642 333
Size: 310 77 7 218 414 248 35 2620
Fragments arranged by size:
2620 414 310 248 218 77 35 7
BanII G_rGCy'C
Cuts at: 362 653 943 1973 362
Size: 291 290 1030 2318
Fragments arranged by size:
2318 1030 291 290
BbeI G_GCGC'C
Cuts at: 337 647 724 731 1363 1611 337
Size: 310 77 7 632 248 2655
Fragments arranged by size:
2655 632 310 248 77 7
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2883 3929 1827
Size: 1056 1046 1827
Fragments arranged by size:
1827 1056 1046
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 171 907 171
Size: 736 3193
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 362 653 1554 1647 2771 362
Size: 291 901 93 1124 1520
Fragments arranged by size:
1520 1124 901 291 93
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2698 2996 1105
Size: 879 714 298 2038
Fragments arranged by size:
2038 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3633 3633
Size: 3929
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 334 401 488 644 721 728 1360 1608 2310
Size: 67 87 156 77 7 632 248 702
Cuts at: 2310 3806 334
Size: 1496 457
Fragments arranged by size:
1496 702 632 457 248 156 87 77
67 7
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2728 2875 3149 1118
Size: 521 678 411 147 274 1898
Fragments arranged by size:
1898 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 225 228 231 234 237 240 249 258 270
Size: 3 3 3 3 3 9 9 12
Cuts at: 270 273 292 424 225
Size: 3 19 132 3730
Fragments arranged by size:
3730 132 19 12 9 9 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 600 633 1354 2112 2777 3185 600
Size: 33 721 758 665 408 1344
Fragments arranged by size:
1344 758 721 665 408 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 925 828
Size: 97 3832
BsiEI CG_ry'CG
Cuts at: 104 341 397 546 1471 1517 2747 104
Size: 237 56 149 925 46 1230 1286
Fragments arranged by size:
1286 1230 925 237 149 56 46
BsiHKAI G_wGCw'C
Cuts at: 943 1721 1911 2771 943
Size: 778 190 860 2101
Fragments arranged by size:
2101 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 362 653 943 1554 1647 1721 1911 1973 2771
Size: 291 290 611 93 74 190 62 798
Cuts at: 2771 362
Size: 1520
Fragments arranged by size:
1520 798 611 291 290 190 93 74
62
BspHI T'CATG_A
Cuts at: 2361 2361
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 856 979 1193 1495 1876 856
Size: 123 214 302 381 2909
Fragments arranged by size:
2909 381 302 214 123
BsrBI CCG'CTC
Cuts at: 50 453 2221 2275 3759 3924 50
Size: 403 1768 54 1484 165 55
Fragments arranged by size:
1768 1484 403 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 336 1927 2108 336
Size: 1591 181 2157
Fragments arranged by size:
2157 1591 181
BssHII G'CGCG_C
Cuts at: 130 316 371 536 629 130
Size: 186 55 165 93 3430
Fragments arranged by size:
3430 186 165 93 55
BssSI C'ACGA_G
Cuts at: 2200 2908 2200
Size: 708 3221
BstAPI GCAn_nnn'nTGC
Cuts at: 426 621 1306 426
Size: 195 685 3049
Fragments arranged by size:
3049 685 195
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstYI r'GATC_y
Cuts at: 1 954 1421 1444 1779 2025 2331 2343 2429
Size: 953 467 23 335 246 306 12 86
Cuts at: 2429 3481 1
Size: 1052 449
Fragments arranged by size:
1052 953 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 482 482
Size: 3929
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 525 1172 525
Size: 647 3282
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2979 1635
Size: 1344 2585
EaeI y'GGCC_r
Cuts at: 163 338 394 479 543 674 798 881 896
Size: 175 56 85 64 131 124 83 15
Cuts at: 896 1514 1688 2079 2106 3451 163
Size: 618 174 391 27 1345 641
Fragments arranged by size:
1345 641 618 391 175 174 131 124
85 83 64 56 27 15
EagI C'GGCC_G
Cuts at: 338 394 543 1514 338
Size: 56 149 971 2753
Fragments arranged by size:
2753 971 149 56
EarI CTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2879 3025 3739 2137
Size: 742 146 714 2327
Fragments arranged by size:
2327 742 714 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1754 2186 2539 3201 332
Size: 1422 432 353 662 1060
Fragments arranged by size:
1422 1060 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 307 496 307
Size: 189 3740
EcoO109I rG'GnC_Cy
Cuts at: 194 352 468 736 1068 194
Size: 158 116 268 332 3055
Fragments arranged by size:
3055 332 268 158 116
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 337 392 647 724 731 781 1363 1611 2841
Size: 55 255 77 7 50 582 248 1230
Cuts at: 2841 3612 337
Size: 771 654
Fragments arranged by size:
1230 771 654 582 255 248 77 55
50 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1771 1801 1803 1833 1771
Size: 30 2 30 3867
Fragments arranged by size:
3867 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 333 643 720 727 1359 1607 333
Size: 310 77 7 632 248 2655
Fragments arranged by size:
2655 632 310 248 77 7
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2688 2872 2688
Size: 184 3745
MscI TGG'CCA
Cuts at: 481 1690 481
Size: 1209 2720
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 171 426 520 1040 1354 1714 2498 2743 171
Size: 255 94 520 314 360 784 245 1357
Fragments arranged by size:
1357 784 520 360 314 255 245 94
NaeI GCC'GGC
Cuts at: 338 2110 338
Size: 1772 2157
NarI GG'CG_CC
Cuts at: 334 644 721 728 1360 1608 334
Size: 310 77 7 632 248 2655
Fragments arranged by size:
2655 632 310 248 77 7
NcoI C'CATG_G
Cuts at: 482 482
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 336 2108 336
Size: 1772 2157
NspI r_CATG'y
Cuts at: 465 982 1482 3085 465
Size: 517 500 1603 1309
Fragments arranged by size:
1603 1309 517 500
PciI A'CATG_T
Cuts at: 3081 3081
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2375 2407 2375
Size: 32 3897
PpuMI rG'GwC_Cy
Cuts at: 468 736 468
Size: 268 3661
PspOMI G'GGCC_C
Cuts at: 358 649 358
Size: 291 3638
PstI C_TGCA'G
Cuts at: 617 976 1661 617
Size: 359 685 2885
Fragments arranged by size:
2885 685 359
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 426 1354 1714 426
Size: 928 360 2641
Fragments arranged by size:
2641 928 360
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SexAI A'CCwGG_T
Cuts at: 872 872
Size: 3929
SfcI C'TryA_G
Cuts at: 33 219 613 972 1657 2625 2816 33
Size: 186 394 359 685 968 191 1146
Fragments arranged by size:
1146 968 685 394 359 191 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 171 171
Size: 3929
SfoI GGC'GCC
Cuts at: 335 645 722 729 1361 1609 335
Size: 310 77 7 632 248 2655
Fragments arranged by size:
2655 632 310 248 77 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 150 357 363 380 951 150
Size: 207 6 17 571 3128
Fragments arranged by size:
3128 571 207 17 6
SmlI C'TyrA_G
Cuts at: 1084 1999 2713 2975 1084
Size: 915 714 262 2038
Fragments arranged by size:
2038 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 465 982 1482 465
Size: 517 500 2912
Fragments arranged by size:
2912 517 500
StyI C'CwwG_G
Cuts at: 308 404 482 920 1063 1417 308
Size: 96 78 438 143 354 2820
Fragments arranged by size:
2820 438 354 143 96 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 110 3444 110
Size: 3334 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 1424 891
Size: 533 3396
XmaI C'CCGG_G
Cuts at: 148 355 361 378 949 148
Size: 207 6 17 571 3128
Fragments arranged by size:
3128 571 207 17 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BspEI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI