Restriction map for
pF1KB5280
(Circular) (Six-base) MAPSORT of: pF1KB5280.seq Check: 7238 from: 1 to: 3851
pF1KB5280 3851 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 845 3691 845
Size: 2846 1005
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 1676 2108 2461 3123 578
Size: 1098 432 353 662 1306
Fragments arranged by size:
1306 1098 662 432 353
AfeI AGC'GCT
Cuts at: 163 163
Size: 3851
AflIII A'CryG_T
Cuts at: 1203 3003 3284 1203
Size: 1800 281 1770
Fragments arranged by size:
1800 1770 281
AlwNI CAG_nnn'CTG
Cuts at: 129 783 1156 2594 129
Size: 654 373 1438 1386
Fragments arranged by size:
1438 1386 654 373
ApaLI G'TGCA_C
Cuts at: 2689 2689
Size: 3851
ApoI r'AATT_y
Cuts at: 855 3204 3460 855
Size: 2349 256 1246
Fragments arranged by size:
2349 1246 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 871 871
Size: 3851
BamHI G'GATC_C
Cuts at: 876 876
Size: 3851
BanI G'GyrC_C
Cuts at: 268 867 1281 1529 1564 268
Size: 599 414 248 35 2555
Fragments arranged by size:
2555 599 414 248 35
BanII G_rGCy'C
Cuts at: 865 1895 865
Size: 1030 2821
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2805 3851 1749
Size: 1056 1046 1749
Fragments arranged by size:
1749 1056 1046
BclI T'GATC_A
Cuts at: 431 770 1371 431
Size: 339 601 2911
Fragments arranged by size:
2911 601 339
BglI GCCn_nnn'nGGC
Cuts at: 154 154
Size: 3851
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 725 963 725
Size: 238 3613
Bme1580I G_kGCm'C
Cuts at: 1476 1569 2693 1476
Size: 93 1124 2634
Fragments arranged by size:
2634 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 811 2218 811
Size: 1407 2444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 406 306
Size: 100 3751
Bpu10I CC'TnA_GC
Cuts at: 677 677
Size: 3851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2620 2918 1027
Size: 879 714 298 1960
Fragments arranged by size:
1960 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 233 1365 3402 6
Size: 227 1132 2037 455
Fragments arranged by size:
2037 1132 455 227
BsaHI Gr'CG_yC
Cuts at: 1282 1530 2232 3728 1282
Size: 248 702 1496 1405
Fragments arranged by size:
1496 1405 702 248
BsaWI w'CCGG_w
Cuts at: 1040 1561 2239 2650 2797 3071 1040
Size: 521 678 411 147 274 1820
Fragments arranged by size:
1820 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 163
Size: 30 3821
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 3851
BseYI C'CCAG_C
Cuts at: 624 1276 2034 2699 3107 624
Size: 652 758 665 408 1368
Fragments arranged by size:
1368 758 665 652 408
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2669 104
Size: 1289 46 1230 1286
Fragments arranged by size:
1289 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 865 1643 1833 2693 865
Size: 778 190 860 2023
Fragments arranged by size:
2023 860 778 190
BsmI GAATG_Cn'
Cuts at: 675 675
Size: 3851
Bsp1286I G_dGCh'C
Cuts at: 865 1476 1569 1643 1833 1895 2693 865
Size: 611 93 74 190 62 798 2023
Fragments arranged by size:
2023 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2283 2283
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1115 1417 1798 901
Size: 214 302 381 2954
Fragments arranged by size:
2954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 405 450 1763 405
Size: 45 1313 2493
Fragments arranged by size:
2493 1313 45
BsrFI r'CCGG_y
Cuts at: 1849 2030 1849
Size: 181 3670
BssSI C'ACGA_G
Cuts at: 2122 2830 2122
Size: 708 3143
BstAPI GCAn_nnn'nTGC
Cuts at: 129 783 1228 129
Size: 654 445 2752
Fragments arranged by size:
2752 654 445
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstXI CCAn_nnnn'nTGG
Cuts at: 624 624
Size: 3851
BstYI r'GATC_y
Cuts at: 1 662 876 1343 1366 1701 1947 2253 2265
Size: 661 214 467 23 335 246 306 12
Cuts at: 2265 2351 3403 1
Size: 86 1052 449
Fragments arranged by size:
1052 661 467 449 335 306 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 107 317 107
Size: 210 3641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 1094 1094
Size: 3851
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2901 1557
Size: 1344 2507
EaeI y'GGCC_r
Cuts at: 146 1436 1610 2001 2028 3373 146
Size: 1290 174 391 27 1345 624
Fragments arranged by size:
1345 1290 624 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 2059 2801 2947 3661 172
Size: 1887 742 146 714 362
Fragments arranged by size:
1887 742 714 362 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 406 578 1676 2108 2461 3123 306
Size: 100 172 1098 432 353 662 1034
Fragments arranged by size:
1098 1034 662 432 353 172 100
EcoO109I rG'GnC_Cy
Cuts at: 990 990
Size: 3851
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 594 1870 594
Size: 1276 2575
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 165 1285 1533 2763 3534 165
Size: 1120 248 1230 771 482
Fragments arranged by size:
1230 1120 771 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 394 426 1693 1723 1725 1755 163
Size: 32 199 32 1267 30 2 30 2259
Fragments arranged by size:
2259 1267 199 32 32 30 30 2
HincII GTy'rAC
Cuts at: 890 890
Size: 3851
HindIII A'AGCT_T
Cuts at: 356 551 356
Size: 195 3656
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2610 2794 2610
Size: 184 3667
MscI TGG'CCA
Cuts at: 1612 1612
Size: 3851
MslI CAynn'nnrTG
Cuts at: 1840 1967 1840
Size: 127 3724
MspA1I CmG'CkG
Cuts at: 126 135 962 1276 1636 2420 2665 126
Size: 9 827 314 360 784 245 1312
Fragments arranged by size:
1312 827 784 360 314 245 9
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NcoI C'CATG_G
Cuts at: 107 317 107
Size: 210 3641
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 904 1404 3007 904
Size: 500 1603 1748
Fragments arranged by size:
1748 1603 500
PciI A'CATG_T
Cuts at: 3003 3003
Size: 3851
PfoI T'CCnGG_A
Cuts at: 283 283
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2297 2329 2297
Size: 32 3819
PstI C_TGCA'G
Cuts at: 134 898 1583 134
Size: 764 685 2402
Fragments arranged by size:
2402 764 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 126 1276 1636 126
Size: 1150 360 2341
Fragments arranged by size:
2341 1150 360
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SfcI C'TryA_G
Cuts at: 33 130 894 1579 2547 2738 33
Size: 97 764 685 968 191 1146
Fragments arranged by size:
1146 968 764 685 191 97
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 873 873
Size: 3851
SmlI C'TyrA_G
Cuts at: 1006 1921 2635 2897 1006
Size: 915 714 262 1960
Fragments arranged by size:
1960 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StyI C'CwwG_G
Cuts at: 107 317 985 1339 107
Size: 210 668 354 2619
Fragments arranged by size:
2619 668 354 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1434 1567 2026 2235 1434
Size: 133 459 209 3050
Fragments arranged by size:
3050 459 209 133
TatI w'GTAC_w
Cuts at: 3366 3366
Size: 3851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 3819
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 726 1346 726
Size: 620 3231
XmaI C'CCGG_G
Cuts at: 871 871
Size: 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI