Restriction map for
pF1KB5278
(Circular) (Six-base) MAPSORT of: pF1KB5278.seq Check: 4569 from: 1 to: 4238
pF1KB5278 4238 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 836 1276 836
Size: 440 3798
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 2063 2495 3242 3510 365
Size: 1698 432 747 268 1093
Fragments arranged by size:
1698 1093 747 432 268
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AleI CACnn'nnGTG
Cuts at: 889 1046 889
Size: 157 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 154 154
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 1543 3110 1543
Size: 1567 2671
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 669 1242 3591 3847 669
Size: 573 2349 256 1060
Fragments arranged by size:
2349 1060 573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 736 887 1258 736
Size: 151 371 3716
Fragments arranged by size:
3716 371 151
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 742 1254 1668 1916 1951 742
Size: 512 414 248 35 3029
Fragments arranged by size:
3029 512 414 248 35
BanII G_rGCy'C
Cuts at: 905 1252 2282 905
Size: 347 1030 2861
Fragments arranged by size:
2861 1030 347
BbeI G_GCGC'C
Cuts at: 746 1672 1920 746
Size: 926 248 3064
Fragments arranged by size:
3064 926 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1212 1246 1212
Size: 34 4204
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 512 1758 512
Size: 1246 2992
BglI GCCn_nnn'nGGC
Cuts at: 989 989
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 896 1863 1956 3012 896
Size: 967 93 1056 2122
Fragments arranged by size:
2122 1056 967 93
BmgBI CAC'GTC
Cuts at: 702 702
Size: 4238
BmrI ACTGGGnnnn_n'
Cuts at: 175 1450 1531 1866 4010 175
Size: 1275 81 335 2144 403
Fragments arranged by size:
2144 1275 403 335 81
BmtI G_CTAG'C
Cuts at: 145 145
Size: 4238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 2605 237
Size: 2368 1870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4238
Bpu10I CC'TnA_GC
Cuts at: 758 758
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1111 3942 1111
Size: 2831 1407
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 916 1752 3789 6
Size: 910 836 2037 455
Fragments arranged by size:
2037 910 836 455
BsaHI Gr'CG_yC
Cuts at: 743 1669 1917 2619 4115 743
Size: 926 248 702 1496 866
Fragments arranged by size:
1496 926 866 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 188 218 188
Size: 30 4208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 4238
BseYI C'CCAG_C
Cuts at: 1663 2421 2998 3494 1663
Size: 758 577 496 2407
Fragments arranged by size:
2407 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1090
Size: 4238
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1090 1252 2030 2220 3012 1090
Size: 162 778 190 792 2316
Fragments arranged by size:
2316 792 778 190 162
Bsp1286I G_dGCh'C
Cuts at: 896 905 1090 1252 1863 1956 2030 2220 2282
Size: 9 185 162 611 93 74 190 62
Cuts at: 2282 3012 896
Size: 730 2122
Fragments arranged by size:
2122 730 611 190 185 162 93 74
62 9
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1288 1502 1804 2185 1288
Size: 214 302 381 3341
Fragments arranged by size:
3341 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 606 2150 606
Size: 1544 2694
BsrFI r'CCGG_y
Cuts at: 793 2236 2417 2626 793
Size: 1443 181 209 2405
Fragments arranged by size:
2405 1443 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 1615 1615
Size: 4238
BstBI TT'CG_AA
Cuts at: 915 2599 915
Size: 1684 2554
BstXI CCAn_nnnn'nTGG
Cuts at: 231 435 231
Size: 204 4034
BstYI r'GATC_y
Cuts at: 1 1263 1730 1753 2088 2334 3346 3432 3444
Size: 1262 467 23 335 246 1012 86 12
Cuts at: 3444 3790 1
Size: 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1031 107
Size: 924 3314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 718 1023 1031 1230 1481 718
Size: 305 8 199 251 3475
Fragments arranged by size:
3475 305 251 199 8
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 395 881 1823 1997 2388 2415 3760 395
Size: 486 942 174 391 27 1345 873
Fragments arranged by size:
1345 942 873 486 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 365 2063 2495 3242 3510 190
Size: 175 1698 432 747 268 918
Fragments arranged by size:
1698 918 747 432 268 175
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 669 1242 669
Size: 573 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 216 2257 216
Size: 2041 2197
FspI TGC'GCA
Cuts at: 130 724 2019 130
Size: 594 1295 2349
Fragments arranged by size:
2349 1295 594
HaeII r_GCGC'y
Cuts at: 746 1672 1920 2942 3921 746
Size: 926 248 1022 979 1063
Fragments arranged by size:
1063 1022 979 926 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 483 515 2080 2110 2112 2142 483
Size: 32 1565 30 2 30 2579
Fragments arranged by size:
2579 1565 32 30 30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
KasI G'GCGC_C
Cuts at: 742 1668 1916 742
Size: 926 248 3064
Fragments arranged by size:
3064 926 248
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 883 1999 883
Size: 1116 3122
MslI CAynn'nnrTG
Cuts at: 592 658 889 1046 1112 2227 2354 592
Size: 66 231 157 66 1115 127 2476
Fragments arranged by size:
2476 1115 231 157 127 66 66
MspA1I CmG'CkG
Cuts at: 253 575 584 753 1349 1663 2023 3036 3281
Size: 322 9 169 596 314 360 1013 245
Cuts at: 3281 253
Size: 1210
Fragments arranged by size:
1210 1013 596 360 322 314 245 169
9
NaeI GCC'GGC
Cuts at: 795 2419 795
Size: 1624 2614
NarI GG'CG_CC
Cuts at: 743 1669 1917 743
Size: 926 248 3064
Fragments arranged by size:
3064 926 248
NcoI C'CATG_G
Cuts at: 107 1031 107
Size: 924 3314
NgoMIV G'CCGG_C
Cuts at: 793 2417 793
Size: 1624 2614
NheI G'CTAG_C
Cuts at: 141 141
Size: 4238
NspI r_CATG'y
Cuts at: 1291 1791 2698 1291
Size: 500 907 2831
Fragments arranged by size:
2831 907 500
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PfoI T'CCnGG_A
Cuts at: 434 467 938 1147 434
Size: 33 471 209 3525
Fragments arranged by size:
3525 471 209 33
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PspXI vC'TCGA_Gb
Cuts at: 887 887
Size: 4238
PstI C_TGCA'G
Cuts at: 1285 1970 1285
Size: 685 3553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 1281 1966 2959 3150 33
Size: 1248 685 993 191 1121
Fragments arranged by size:
1248 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 989 989
Size: 4238
SfoI GGC'GCC
Cuts at: 744 1670 1918 744
Size: 926 248 3064
Fragments arranged by size:
3064 926 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 887 1393 2308 2800 3062 887
Size: 506 915 492 262 2063
Fragments arranged by size:
2063 915 506 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StyI C'CwwG_G
Cuts at: 107 560 896 1011 1031 1372 1726 107
Size: 453 336 115 20 341 354 2619
Fragments arranged by size:
2619 453 354 341 336 115 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 1107 1821 1954 2413 2622 441
Size: 666 714 133 459 209 2057
Fragments arranged by size:
2057 714 666 459 209 133
TatI w'GTAC_w
Cuts at: 3753 3753
Size: 4238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 416 448 802 834 3755 3787 416
Size: 32 354 32 2921 32 867
Fragments arranged by size:
2921 867 354 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 624 1733 114
Size: 510 1109 2619
Fragments arranged by size:
2619 1109 510
XhoI C'TCGA_G
Cuts at: 887 887
Size: 4238
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI