Restriction map for
pF1KB5275
(Circular) (Six-base) MAPSORT of: pF1KB5275.seq Check: 4158 from: 1 to: 4520
pF1KB5275 4520 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1310 1558 1310
Size: 248 4272
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1383 2345 2777 3130 3792 1383
Size: 962 432 353 662 2111
Fragments arranged by size:
2111 962 662 432 353
AflIII A'CryG_T
Cuts at: 554 1872 3672 3953 554
Size: 1318 1800 281 1121
Fragments arranged by size:
1800 1318 1121 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 772 772
Size: 4520
AlwNI CAG_nnn'CTG
Cuts at: 759 1243 1825 3263 759
Size: 484 582 1438 2016
Fragments arranged by size:
2016 1438 582 484
ApaI G_GGCC'C
Cuts at: 138 315 1513 138
Size: 177 1198 3145
Fragments arranged by size:
3145 1198 177
ApaLI G'TGCA_C
Cuts at: 3358 3358
Size: 4520
ApoI r'AATT_y
Cuts at: 1524 3873 4129 1524
Size: 2349 256 1915
Fragments arranged by size:
2349 1915 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 182 824 1464 1540 182
Size: 642 640 76 3162
Fragments arranged by size:
3162 642 640 76
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 186 544 748 1536 1950 2198 2233 186
Size: 358 204 788 414 248 35 2473
Fragments arranged by size:
2473 788 414 358 248 204 35
BanII G_rGCy'C
Cuts at: 138 315 1513 1534 2564 138
Size: 177 1198 21 1030 2094
Fragments arranged by size:
2094 1198 1030 177 21
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BbvCI CC'TCA_GC
Cuts at: 850 850
Size: 4520
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3474 4520 2418
Size: 1056 1046 2418
Fragments arranged by size:
2418 1056 1046
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglI GCCn_nnn'nGGC
Cuts at: 133 722 746 133
Size: 589 24 3907
Fragments arranged by size:
3907 589 24
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 964 1632 964
Size: 668 3852
Bme1580I G_kGCm'C
Cuts at: 138 315 394 751 980 1303 1513 2145 2238
Size: 177 79 357 229 323 210 632 93
Cuts at: 2238 3362 138
Size: 1124 1296
Fragments arranged by size:
1296 1124 632 357 323 229 210 177
93 79
BmgBI CAC'GTC
Cuts at: 555 819 987 555
Size: 264 168 4088
Fragments arranged by size:
4088 264 168
BmrI ACTGGGnnnn_n'
Cuts at: 333 1732 1813 2148 4292 333
Size: 1399 81 335 2144 561
Fragments arranged by size:
2144 1399 561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1066 1222 322
Size: 744 156 3620
Fragments arranged by size:
3620 744 156
Bpu10I CC'TnA_GC
Cuts at: 850 850
Size: 4520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1696 2575 3289 3587 1696
Size: 879 714 298 2629
Fragments arranged by size:
2629 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 285 4224 285
Size: 3939 581
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 1951 2199 2901 4397 1951
Size: 248 702 1496 2074
Fragments arranged by size:
2074 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1709 2230 2908 3319 3466 3740 1709
Size: 521 678 411 147 274 2489
Fragments arranged by size:
2489 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 326 356 193
Size: 30 103 30 4357
Fragments arranged by size:
4357 103 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 385 460 461 838 201
Size: 184 75 1 377 3883
Fragments arranged by size:
3883 377 184 75 1
BseYI C'CCAG_C
Cuts at: 314 464 744 752 1028 1330 1506 1945 2703
Size: 150 280 8 276 302 176 439 758
Cuts at: 2703 3368 3776 314
Size: 665 408 1058
Fragments arranged by size:
1058 758 665 439 408 302 280 276
176 150 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 825 825
Size: 4520
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3338 104
Size: 1958 46 1230 1286
Fragments arranged by size:
1958 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 452 1534 2312 2502 3362 452
Size: 1082 778 190 860 1610
Fragments arranged by size:
1610 1082 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 599 825 599
Size: 226 4294
Bsp1286I G_dGCh'C
Cuts at: 138 315 394 452 751 980 1303 1513 1534
Size: 177 79 58 299 229 323 210 21
Cuts at: 1534 2145 2238 2312 2502 2564 3362 138
Size: 611 93 74 190 62 798 1296
Fragments arranged by size:
1296 798 611 323 299 229 210 190
177 93 79 74 62 58 21
BspHI T'CATG_A
Cuts at: 2952 2952
Size: 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 1439 1570 1784 2086 2467 464
Size: 975 131 214 302 381 2517
Fragments arranged by size:
2517 975 381 302 214 131
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2432 2432
Size: 4520
BsrFI r'CCGG_y
Cuts at: 248 2518 2699 248
Size: 2270 181 2069
Fragments arranged by size:
2270 2069 181
BssSI C'ACGA_G
Cuts at: 2791 3499 2791
Size: 708 3812
BstAPI GCAn_nnn'nTGC
Cuts at: 456 1897 456
Size: 1441 3079
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstEII G'GTnAC_C
Cuts at: 1089 1089
Size: 4520
BstYI r'GATC_y
Cuts at: 1 950 1196 1545 2012 2035 2370 2616 2922
Size: 949 246 349 467 23 335 246 306
Cuts at: 2922 2934 3020 4072 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 949 467 449 349 335 306 246
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 368 1361 368
Size: 993 3527
BtgI C'CryG_G
Cuts at: 980 980
Size: 4520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 2651 2814 2470
Size: 181 163 4176
Fragments arranged by size:
4176 181 163
BtsI GCAGTG_nn'
Cuts at: 336 808 1064 1763 336
Size: 472 256 699 3093
Fragments arranged by size:
3093 699 472 256
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 505 2226 3570 505
Size: 1721 1344 1455
Fragments arranged by size:
1721 1455 1344
EaeI y'GGCC_r
Cuts at: 1061 2105 2279 2670 2697 4042 1061
Size: 1044 174 391 27 1345 1539
Fragments arranged by size:
1539 1345 1044 391 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3470 3616 4330 2728
Size: 742 146 714 2918
Fragments arranged by size:
2918 742 714 146
EcoICRI GAG'CTC
Cuts at: 1532 1532
Size: 4520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1066 1222 1383 2345 2777 3130 3792 322
Size: 744 156 161 962 432 353 662 1050
Fragments arranged by size:
1050 962 744 662 432 353 161 156
EcoNI CCTnn'n_nnAGG
Cuts at: 433 433
Size: 4520
EcoO109I rG'GnC_Cy
Cuts at: 134 1357 1510 1659 134
Size: 1223 153 149 2995
Fragments arranged by size:
2995 1223 153 149
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4520
FspI TGC'GCA
Cuts at: 1435 2301 1435
Size: 866 3654
HaeII r_GCGC'y
Cuts at: 812 971 1954 2202 3432 4203 812
Size: 159 983 248 1230 771 1129
Fragments arranged by size:
1230 1129 983 771 248 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2362 2392 2394 2424 2362
Size: 30 2 30 4458
Fragments arranged by size:
4458 30 30 2
HincII GTy'rAC
Cuts at: 1559 1559
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3279 3463 3279
Size: 184 4336
MscI TGG'CCA
Cuts at: 1063 2281 1063
Size: 1218 3302
MslI CAynn'nnrTG
Cuts at: 266 286 494 553 2509 2636 266
Size: 20 208 59 1956 127 2150
Fragments arranged by size:
2150 1956 208 127 59 20
MspA1I CmG'CkG
Cuts at: 414 756 963 1059 1086 1631 1945 2305 3089
Size: 342 207 96 27 545 314 360 784
Cuts at: 3089 3334 414
Size: 245 1600
Fragments arranged by size:
1600 784 545 360 342 314 245 207
96 27
NaeI GCC'GGC
Cuts at: 250 2701 250
Size: 2451 2069
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NgoMIV G'CCGG_C
Cuts at: 248 2699 248
Size: 2451 2069
NspI r_CATG'y
Cuts at: 1053 1309 1573 2073 3676 1053
Size: 256 264 500 1603 1897
Fragments arranged by size:
1897 1603 500 264 256
PciI A'CATG_T
Cuts at: 3672 3672
Size: 4520
PflMI CCAn_nnn'nTGG
Cuts at: 759 759
Size: 4520
PfoI T'CCnGG_A
Cuts at: 299 1199 299
Size: 900 3620
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 4488
PpuMI rG'GwC_Cy
Cuts at: 1357 1357
Size: 4520
PspOMI G'GGCC_C
Cuts at: 134 311 1509 134
Size: 177 1198 3145
Fragments arranged by size:
3145 1198 177
PstI C_TGCA'G
Cuts at: 365 419 527 962 1567 2252 365
Size: 54 108 435 605 685 2633
Fragments arranged by size:
2633 685 605 435 108 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 756 963 1086 1945 2305 756
Size: 207 123 859 360 2971
Fragments arranged by size:
2971 859 360 207 123
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 1534 1534
Size: 4520
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 361 415 523 958 1563 2248 3216 3407
Size: 328 54 108 435 605 685 968 191
Cuts at: 3407 33
Size: 1146
Fragments arranged by size:
1146 968 685 605 435 328 191 108
54
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 184 826 1542 184
Size: 642 716 3162
Fragments arranged by size:
3162 716 642
SmlI C'TyrA_G
Cuts at: 1675 2590 3304 3566 1675
Size: 915 714 262 2629
Fragments arranged by size:
2629 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1573 2073 1573
Size: 500 4020
StuI AGG'CCT
Cuts at: 1499 1499
Size: 4520
StyI C'CwwG_G
Cuts at: 1654 2008 1654
Size: 354 4166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 506 709 766 877 2103 2236 2695 2904 506
Size: 203 57 111 1226 133 459 209 2122
Fragments arranged by size:
2122 1226 459 209 203 133 111 57
TatI w'GTAC_w
Cuts at: 4035 4035
Size: 4520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 326 358 1136 1168 4037 4069 206
Size: 32 88 32 778 32 2869 32 657
Fragments arranged by size:
2869 778 657 88 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2317 2317
Size: 4520
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1083 2015 1083
Size: 932 3588
XmaI C'CCGG_G
Cuts at: 182 824 1540 182
Size: 642 716 3162
Fragments arranged by size:
3162 716 642
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI
BsmI BspEI BsrGI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI