Restriction map for
pF1KB5274
(Circular) (Six-base) MAPSORT of: pF1KB5274.seq Check: 6293 from: 1 to: 4160
pF1KB5274 4160 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 311 1198 311
Size: 887 3273
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1985 2417 2770 3432 650
Size: 1335 432 353 662 1378
Fragments arranged by size:
1378 1335 662 432 353
AflIII A'CryG_T
Cuts at: 1512 3312 3593 1512
Size: 1800 281 2079
Fragments arranged by size:
2079 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 628 1465 2903 628
Size: 837 1438 1885
Fragments arranged by size:
1885 1438 837
ApaLI G'TGCA_C
Cuts at: 2998 2998
Size: 4160
ApoI r'AATT_y
Cuts at: 947 1164 3513 3769 947
Size: 217 2349 256 1338
Fragments arranged by size:
2349 1338 256 217
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 1180 1180
Size: 4160
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 1082 1174 2204 1082
Size: 92 1030 3038
Fragments arranged by size:
3038 1030 92
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 562 1100 562
Size: 538 3622
BbvCI CC'TCA_GC
Cuts at: 784 784
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 405 2058 3114 4160 405
Size: 1653 1056 1046 405
Fragments arranged by size:
1653 1056 1046 405
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglII A'GATC_T
Cuts at: 861 1675 3712 861
Size: 814 2037 1309
Fragments arranged by size:
2037 1309 814
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 1785 1878 3002 1785
Size: 93 1124 2943
Fragments arranged by size:
2943 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1372 1453 1788 3932 1372
Size: 81 335 2144 1600
Fragments arranged by size:
2144 1600 335 81
BmtI G_CTAG'C
Cuts at: 446 446
Size: 4160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 163 569 695 886 155
Size: 8 406 126 191 3429
Fragments arranged by size:
3429 406 191 126 8
Bpu10I CC'TnA_GC
Cuts at: 784 784
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1033 1336 2215 2929 3227 208
Size: 825 303 879 714 298 1141
Fragments arranged by size:
1141 879 825 714 303 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 982 3864 805
Size: 177 2882 1101
Fragments arranged by size:
2882 1101 177
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 410 416 1674 3711 6
Size: 404 6 1258 2037 455
Fragments arranged by size:
2037 1258 455 404 6
BsaHI Gr'CG_yC
Cuts at: 261 1591 1839 2541 4037 261
Size: 1330 248 702 1496 384
Fragments arranged by size:
1496 1330 702 384 248
BsaWI w'CCGG_w
Cuts at: 551 1349 1870 2548 2959 3106 3380 551
Size: 798 521 678 411 147 274 1331
Fragments arranged by size:
1331 798 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 136
Size: 30 4130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 122 445 1144 122
Size: 323 699 3138
Fragments arranged by size:
3138 699 323
BseYI C'CCAG_C
Cuts at: 815 1585 2343 3008 3416 815
Size: 770 758 665 408 1559
Fragments arranged by size:
1559 770 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2978 104
Size: 1598 46 1230 1286
Fragments arranged by size:
1598 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1082 1174 1952 2142 3002 1082
Size: 92 778 190 860 2240
Fragments arranged by size:
2240 860 778 190 92
Bsp1286I G_dGCh'C
Cuts at: 1082 1174 1785 1878 1952 2142 2204 3002 1082
Size: 92 611 93 74 190 62 798 2240
Fragments arranged by size:
2240 798 611 190 93 92 74 62
BspEI T'CCGG_A
Cuts at: 551 551
Size: 4160
BspHI T'CATG_A
Cuts at: 1025 2592 1025
Size: 1567 2593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 323 1210 1424 1726 2107 323
Size: 887 214 302 381 2376
Fragments arranged by size:
2376 887 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 2158 2339 2158
Size: 181 3979
BssSI C'ACGA_G
Cuts at: 2431 3139 2431
Size: 708 3452
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 936 936
Size: 4160
BstYI r'GATC_y
Cuts at: 1 861 1017 1185 1652 1675 2010 2256 2562
Size: 860 156 168 467 23 335 246 306
Cuts at: 2562 2574 2660 3712 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 860 467 449 335 306 246 168
156 86 23 12
BtgI C'CryG_G
Cuts at: 507 507
Size: 4160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 801 1403 801
Size: 602 3558
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 3210 1866
Size: 1344 2816
EaeI y'GGCC_r
Cuts at: 1745 1919 2310 2337 3682 1745
Size: 174 391 27 1345 2223
Fragments arranged by size:
2223 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 535 711 2183 2393 535
Size: 176 1472 210 2302
Fragments arranged by size:
2302 1472 210 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 3110 3256 3970 2368
Size: 742 146 714 2558
Fragments arranged by size:
2558 742 714 146
EcoICRI GAG'CTC
Cuts at: 1080 1172 1080
Size: 92 4068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 163 569 650 695 886 1985 2417 2770
Size: 8 406 81 45 191 1099 432 353
Cuts at: 2770 3432 155
Size: 662 883
Fragments arranged by size:
1099 883 662 432 406 353 191 81
45 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1150 1150
Size: 4160
EcoO109I rG'GnC_Cy
Cuts at: 1299 1299
Size: 4160
EcoRI G'AATT_C
Cuts at: 947 1164 947
Size: 217 3943
EcoRV GAT'ATC
Cuts at: 378 702 378
Size: 324 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 4160
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 1594 1842 3072 3843 1594
Size: 248 1230 771 1911
Fragments arranged by size:
1911 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 804 836 1123 1155 2002 2032 2034 2064 804
Size: 32 287 32 847 30 2 30 2900
Fragments arranged by size:
2900 847 287 32 32 30 30 2
HincII GTy'rAC
Cuts at: 219 1199 219
Size: 980 3180
HindIII A'AGCT_T
Cuts at: 333 333
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MfeI C'AATT_G
Cuts at: 239 239
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2919 3103 2919
Size: 184 3976
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 254 824 1030 2149 2276 254
Size: 570 206 1119 127 2138
Fragments arranged by size:
2138 1119 570 206 127
MspA1I CmG'CkG
Cuts at: 373 936 1271 1585 1945 2729 2974 373
Size: 563 335 314 360 784 245 1559
Fragments arranged by size:
1559 784 563 360 335 314 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NheI G'CTAG_C
Cuts at: 442 442
Size: 4160
NspI r_CATG'y
Cuts at: 1213 1713 3316 1213
Size: 500 1603 2057
Fragments arranged by size:
2057 1603 500
PciI A'CATG_T
Cuts at: 3312 3312
Size: 4160
PfoI T'CCnGG_A
Cuts at: 711 711
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 4128
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 373 936 1585 1945 373
Size: 563 649 360 2588
Fragments arranged by size:
2588 649 563 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1082 1174 1082
Size: 92 4068
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 642 3677 642
Size: 3035 1125
SfcI C'TryA_G
Cuts at: 33 877 1203 1888 2856 3047 33
Size: 844 326 685 968 191 1146
Fragments arranged by size:
1146 968 844 685 326 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1182 1182
Size: 4160
SmlI C'TyrA_G
Cuts at: 223 1048 1315 2230 2944 3206 223
Size: 825 267 915 714 262 1177
Fragments arranged by size:
1177 915 825 714 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
StuI AGG'CCT
Cuts at: 695 695
Size: 4160
StyI C'CwwG_G
Cuts at: 1294 1648 1294
Size: 354 3806
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1112 1743 1876 2335 2544 1112
Size: 631 133 459 209 2728
Fragments arranged by size:
2728 631 459 209 133
TatI w'GTAC_w
Cuts at: 201 619 640 1068 3675 201
Size: 418 21 428 2607 686
Fragments arranged by size:
2607 686 428 418 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1655 1655
Size: 4160
XmaI C'CCGG_G
Cuts at: 1180 1180
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI
BmgBI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HpaI
NcoI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI