Restriction map for
pF1KB5273
(Circular) (Six-base) MAPSORT of: pF1KB5273.seq Check: 8513 from: 1 to: 3509
pF1KB5273 3509 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 547 547
Size: 3509
Acc65I G'GTAC_C
Cuts at: 525 525
Size: 3509
AclI AA'CG_TT
Cuts at: 3349 3349
Size: 3509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1334 1766 2513 2781 1334
Size: 432 747 268 2062
Fragments arranged by size:
2062 747 432 268
AflIII A'CryG_T
Cuts at: 861 1965 2942 861
Size: 1104 977 1428
Fragments arranged by size:
1428 1104 977
AgeI A'CCGG_T
Cuts at: 1897 1897
Size: 3509
AlwNI CAG_nnn'CTG
Cuts at: 814 2381 814
Size: 1567 1942
ApaLI G'TGCA_C
Cuts at: 480 2279 480
Size: 1799 1710
ApoI r'AATT_y
Cuts at: 118 210 316 513 2862 3118 118
Size: 92 106 197 2349 256 509
Fragments arranged by size:
2349 509 256 197 106 92
AseI AT'TA_AT
Cuts at: 20 20
Size: 3509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3509
AvaI C'yCGr_G
Cuts at: 529 529
Size: 3509
BamHI G'GATC_C
Cuts at: 534 534
Size: 3509
BanI G'GyrC_C
Cuts at: 127 525 939 1187 1222 127
Size: 398 414 248 35 2414
Fragments arranged by size:
2414 414 398 248 35
BanII G_rGCy'C
Cuts at: 523 1553 523
Size: 1030 2479
BbeI G_GCGC'C
Cuts at: 943 1191 943
Size: 248 3261
BciVI GTATCCnnnnn_n'
Cuts at: 1407 2168 3509 1407
Size: 761 1341 1407
Fragments arranged by size:
1407 1341 761
BclI T'GATC_A
Cuts at: 230 1029 230
Size: 799 2710
BglII A'GATC_T
Cuts at: 1024 3061 1024
Size: 2037 1472
BlpI GC'TnA_GC
Cuts at: 621 621
Size: 3509
Bme1580I G_kGCm'C
Cuts at: 130 484 1134 1227 2283 130
Size: 354 650 93 1056 1356
Fragments arranged by size:
1356 1056 650 354 93
BmrI ACTGGGnnnn_n'
Cuts at: 214 721 802 1137 3281 214
Size: 507 81 335 2144 442
Fragments arranged by size:
2144 507 442 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1564 2056 2354 685
Size: 879 492 298 1840
Fragments arranged by size:
1840 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3213 3213
Size: 3509
BsaAI yAC'GTr
Cuts at: 862 862
Size: 3509
BsaBI GATnn'nnATC
Cuts at: 6 1023 3060 6
Size: 1017 2037 455
Fragments arranged by size:
2037 1017 455
BsaHI Gr'CG_yC
Cuts at: 940 1188 1890 3386 940
Size: 248 702 1496 1063
Fragments arranged by size:
1496 1063 702 248
BsaWI w'CCGG_w
Cuts at: 698 1219 1897 2171 2318 2729 698
Size: 521 678 274 147 411 1478
Fragments arranged by size:
1478 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 934 1692 2269 2765 934
Size: 758 577 496 1678
Fragments arranged by size:
1678 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3509
BsiEI CG_ry'CG
Cuts at: 104 1051 1097 2305 104
Size: 947 46 1208 1308
Fragments arranged by size:
1308 1208 947 46
BsiHKAI G_wGCw'C
Cuts at: 484 523 1301 1491 2283 484
Size: 39 778 190 792 1710
Fragments arranged by size:
1710 792 778 190 39
Bsp1286I G_dGCh'C
Cuts at: 130 484 523 1134 1227 1301 1491 1553 2283
Size: 354 39 611 93 74 190 62 730
Cuts at: 2283 130
Size: 1356
Fragments arranged by size:
1356 730 611 354 190 93 74 62
39
BspEI T'CCGG_A
Cuts at: 2729 2729
Size: 3509
BspHI T'CATG_A
Cuts at: 2685 2685
Size: 3509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 559 773 1075 1456 559
Size: 214 302 381 2612
Fragments arranged by size:
2612 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1801 1855 3339 3504 50
Size: 1751 54 1484 165 55
Fragments arranged by size:
1751 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1421 1421
Size: 3509
BsrFI r'CCGG_y
Cuts at: 1507 1688 1897 1507
Size: 181 209 3119
Fragments arranged by size:
3119 209 181
BssSI C'ACGA_G
Cuts at: 1780 2138 1780
Size: 358 3151
BstAPI GCAn_nnn'nTGC
Cuts at: 886 886
Size: 3509
BstBI TT'CG_AA
Cuts at: 1870 1870
Size: 3509
BstXI CCAn_nnnn'nTGG
Cuts at: 204 204
Size: 3509
BstYI r'GATC_y
Cuts at: 1 534 1001 1024 1359 1605 2617 2703 2715
Size: 533 467 23 335 246 1012 86 12
Cuts at: 2715 3061 1
Size: 346 449
Fragments arranged by size:
1012 533 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1459 1640 1803 1459
Size: 181 163 3165
Fragments arranged by size:
3165 181 163
BtsI GCAGTG_nn'
Cuts at: 752 752
Size: 3509
DraI TTT'AAA
Cuts at: 282 508 282
Size: 226 3283
DrdI GACnn_nn'nnGTC
Cuts at: 1215 2073 1215
Size: 858 2651
EaeI y'GGCC_r
Cuts at: 215 1094 1268 1659 1686 3031 215
Size: 879 174 391 27 1345 693
Fragments arranged by size:
1345 879 693 391 174 27
EagI C'GGCC_G
Cuts at: 1094 1094
Size: 3509
EarI CTCTTCn'nnn_
Cuts at: 1532 1742 1532
Size: 210 3299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1717 2027 2173 3319 1717
Size: 310 146 1146 1907
Fragments arranged by size:
1907 1146 310 146
EcoICRI GAG'CTC
Cuts at: 521 521
Size: 3509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1334 1766 2513 2781 1334
Size: 432 747 268 2062
Fragments arranged by size:
2062 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 648 648
Size: 3509
EcoRI G'AATT_C
Cuts at: 513 513
Size: 3509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1528 1528
Size: 3509
FspI TGC'GCA
Cuts at: 1290 1290
Size: 3509
HaeII r_GCGC'y
Cuts at: 943 1191 2213 3192 943
Size: 248 1022 979 1260
Fragments arranged by size:
1260 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1381 1383 1413 1351
Size: 30 2 30 3447
Fragments arranged by size:
3447 30 30 2
HincII GTy'rAC
Cuts at: 548 548
Size: 3509
KasI G'GCGC_C
Cuts at: 939 1187 939
Size: 248 3261
KpnI G_GTAC'C
Cuts at: 529 529
Size: 3509
MluI A'CGCG_T
Cuts at: 2942 2942
Size: 3509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2364 2180
Size: 184 3325
MscI TGG'CCA
Cuts at: 217 1270 217
Size: 1053 2456
MslI CAynn'nnrTG
Cuts at: 1498 1625 1498
Size: 127 3382
MspA1I CmG'CkG
Cuts at: 334 620 934 1294 2307 2552 334
Size: 286 314 360 1013 245 1291
Fragments arranged by size:
1291 1013 360 314 286 245
NaeI GCC'GGC
Cuts at: 1690 1690
Size: 3509
NarI GG'CG_CC
Cuts at: 940 1188 940
Size: 248 3261
NgoMIV G'CCGG_C
Cuts at: 1688 1688
Size: 3509
NspI r_CATG'y
Cuts at: 562 1062 1969 562
Size: 500 907 2102
Fragments arranged by size:
2102 907 500
PciI A'CATG_T
Cuts at: 1965 1965
Size: 3509
PmeI GTTT'AAAC
Cuts at: 508 508
Size: 3509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2648 2680 2648
Size: 32 3477
PsiI TTA'TAA
Cuts at: 256 256
Size: 3509
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 258 290 258
Size: 32 3477
PstI C_TGCA'G
Cuts at: 371 556 1241 371
Size: 185 685 2639
Fragments arranged by size:
2639 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3509
PvuII CAG'CTG
Cuts at: 334 934 1294 334
Size: 600 360 2549
Fragments arranged by size:
2549 600 360
RsrII CG'GwC_CG
Cuts at: 1704 1704
Size: 3509
SacI G_AGCT'C
Cuts at: 523 523
Size: 3509
SalI G'TCGA_C
Cuts at: 546 546
Size: 3509
SapI GCTCTTCn'nnn_
Cuts at: 1532 1742 1532
Size: 210 3299
SbfI CC_TGCA'GG
Cuts at: 556 556
Size: 3509
ScaI AGT'ACT
Cuts at: 3026 3026
Size: 3509
SfcI C'TryA_G
Cuts at: 33 367 552 1237 2230 2421 33
Size: 334 185 685 993 191 1121
Fragments arranged by size:
1121 993 685 334 191 185
SfoI GGC'GCC
Cuts at: 941 1189 941
Size: 248 3261
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3509
SmaI CCC'GGG
Cuts at: 531 531
Size: 3509
SmlI C'TyrA_G
Cuts at: 664 1579 2071 2333 664
Size: 915 492 262 1840
Fragments arranged by size:
1840 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3509
SphI G_CATG'C
Cuts at: 562 1062 562
Size: 500 3009
StyI C'CwwG_G
Cuts at: 643 997 643
Size: 354 3155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 1092 1225 1684 1893 169
Size: 923 133 459 209 1785
Fragments arranged by size:
1785 923 459 209 133
TatI w'GTAC_w
Cuts at: 3024 3024
Size: 3509
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3477
Tth111I GACn'n_nGTC
Cuts at: 1306 1306
Size: 3509
XbaI T'CTAG_A
Cuts at: 540 540
Size: 3509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 1004 201
Size: 803 2706
XmaI C'CCGG_G
Cuts at: 529 529
Size: 3509
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI