Restriction map for
pF1KB5264
(Circular) (Six-base) MAPSORT of: pF1KB5264.seq Check: 7013 from: 1 to: 4049
pF1KB5264 4049 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 1006 1087 1006
Size: 81 3968
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 1043 3889 1043
Size: 2846 1203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1874 2306 3053 3321 1045
Size: 829 432 747 268 1773
Fragments arranged by size:
1773 829 747 432 268
AfeI AGC'GCT
Cuts at: 117 117
Size: 4049
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 397 1053 3402 3658 397
Size: 656 2349 256 788
Fragments arranged by size:
2349 788 656 256
AseI AT'TA_AT
Cuts at: 20 587 20
Size: 567 3482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 246 1069 246
Size: 823 3226
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 157 1065 1479 1727 1762 157
Size: 908 414 248 35 2444
Fragments arranged by size:
2444 908 414 248 35
BanII G_rGCy'C
Cuts at: 254 946 1017 1063 2093 254
Size: 692 71 46 1030 2210
Fragments arranged by size:
2210 1030 692 71 46
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BciVI GTATCCnnnnn_n'
Cuts at: 536 913 1947 2708 4049 536
Size: 377 1034 761 1341 536
Fragments arranged by size:
1341 1034 761 536 377
BclI T'GATC_A
Cuts at: 566 1569 566
Size: 1003 3046
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 160 1674 1767 2823 160
Size: 1514 93 1056 1386
Fragments arranged by size:
1514 1386 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 153 1261 1342 1677 3821 153
Size: 1108 81 335 2144 381
Fragments arranged by size:
2144 1108 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 779 1480 1728 2430 3926 779
Size: 701 248 702 1496 902
Fragments arranged by size:
1496 902 702 701 248
BsaWI w'CCGG_w
Cuts at: 833 861 1238 1759 2437 2711 2858 3269 833
Size: 28 377 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 377 274 147 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 775 775
Size: 4049
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 808
Size: 4049
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 859 859
Size: 4049
Bsp1286I G_dGCh'C
Cuts at: 160 254 946 1017 1063 1674 1767 1841 2031
Size: 94 692 71 46 611 93 74 190
Cuts at: 2031 2093 2823 160
Size: 62 730 1386
Fragments arranged by size:
1386 730 692 611 190 94 93 74
71 62 46
BspEI T'CCGG_A
Cuts at: 833 861 3269 833
Size: 28 2408 1613
Fragments arranged by size:
2408 1613 28
BspHI T'CATG_A
Cuts at: 989 3225 989
Size: 2236 1813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 968 1099 1313 1615 1996 968
Size: 131 214 302 381 3021
Fragments arranged by size:
3021 381 302 214 131
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 419 1961 419
Size: 1542 2507
BsrFI r'CCGG_y
Cuts at: 130 2047 2228 2437 130
Size: 1917 181 209 1742
Fragments arranged by size:
1917 1742 209 181
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 643 1074 1541 1564 1899 2145 3157 3243
Size: 642 431 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 642 467 449 431 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 161 107
Size: 54 3995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 684 1292 684
Size: 608 3441
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 1634 1808 2199 2226 3571 1634
Size: 174 391 27 1345 2112
Fragments arranged by size:
2112 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 202 305 2072 2282 202
Size: 103 1767 210 1969
Fragments arranged by size:
1969 1767 210 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1874 2306 3053 3321 1045
Size: 829 432 747 268 1773
Fragments arranged by size:
1773 829 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 757 757
Size: 4049
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 798 2068 798
Size: 1270 2779
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 119 1483 1731 2753 3732 119
Size: 1364 248 1022 979 436
Fragments arranged by size:
1364 1022 979 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 727 759 841 873 911 918 943
Size: 32 274 32 82 32 38 7 25
Cuts at: 943 950 1891 1921 1923 1953 421
Size: 7 941 30 2 30 2517
Fragments arranged by size:
2517 941 274 82 38 32 32 32
30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 429 1088 429
Size: 659 3390
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MfeI C'AATT_G
Cuts at: 616 616
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 496 994 2038 2165 496
Size: 498 1044 127 2380
Fragments arranged by size:
2380 1044 498 127
MspA1I CmG'CkG
Cuts at: 1160 1474 1834 2847 3092 1160
Size: 314 360 1013 245 2117
Fragments arranged by size:
2117 1013 360 314 245
NaeI GCC'GGC
Cuts at: 132 2230 132
Size: 2098 1951
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 107 107
Size: 4049
NdeI CA'TA_TG
Cuts at: 303 303
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 130 2228 130
Size: 2098 1951
NspI r_CATG'y
Cuts at: 687 1102 1602 2509 687
Size: 415 500 907 2227
Fragments arranged by size:
2227 907 500 415
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 202 2072 2282 202
Size: 1870 210 1969
Fragments arranged by size:
1969 1870 210
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 282 3566 282
Size: 3284 765
SexAI A'CCwGG_T
Cuts at: 371 371
Size: 4049
SfcI C'TryA_G
Cuts at: 33 1092 1777 2770 2961 33
Size: 1059 685 993 191 1121
Fragments arranged by size:
1121 1059 993 685 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 248 1071 248
Size: 823 3226
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 687 1102 1602 687
Size: 415 500 3134
Fragments arranged by size:
3134 500 415
SspI AAT'ATT
Cuts at: 585 591 585
Size: 6 4043
StyI C'CwwG_G
Cuts at: 107 1183 1537 107
Size: 1076 354 2619
Fragments arranged by size:
2619 1076 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 1765 2224 2433 1632
Size: 133 459 209 3248
Fragments arranged by size:
3248 459 209 133
TatI w'GTAC_w
Cuts at: 140 280 3564 140
Size: 140 3284 625
Fragments arranged by size:
3284 625 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 246 1069 246
Size: 823 3226
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI